scholarly journals Supplementation of Jengkol Peel on VFA Molar Proportion, Methane Production, and Hydrogen Balance in Vitro

Author(s):  
N. Hidayah ◽  
Nurhaita Nurhaita ◽  
R. Zurina

This research was designed to study about effects of supplementation jengkol (Archidendron jiringa) peel powder on VFA molar proportion, production of methane, and hydrogen balance in vitro. The experiment used randomized block design with 4 treatments (0%, 2%, 4%, 6%) and 4 replications. The research observed of VFA molar proportion, production of methane, and hydrogen balance. Data were analyzed using Analysis of Variance (ANOVA), and the Duncan Multiple Range Test examined the differences among treatment means. The results showed that the supplementation of jengkol (A. jiringa) peel powder until 6% did not affect (P>0.05) VFA molar proportion but the treatment without supplementation of jengkol (A. jiringa) peel powder had the highest (P<0.05) methane and H2 production. It was concluded that the supplementation of jengkol (A. jiringa) peel powder until 6% decreased methane and hydrogen production but did not affect on VFA molar proportion.

2021 ◽  
Vol 19 (1) ◽  
pp. 13
Author(s):  
Nurhaita Nurhaita ◽  
Nur Hidayah ◽  
Neli Definiati

<p class="MDPI17abstract"><strong>Objective: </strong>This research was aimed to evaluation the effect of supplementation jengkol peel powder in fermented oil palm fronds on rumen microbes profile and methane production on <em>in vitro</em>.</p><p class="MDPI17abstract"><strong>Methods: </strong>The used design was factorial randomized block design with four treatments supplementation of jengkol peel powder at 0, 2, 4, and 6% and four groups as replications. The observed variables were rumen microbes profile (population of total bacteria, cellulolytic, and protozoa), methane production, partial <em>volatile fatty acid</em> (VFA) proportion and acetate/propionate (A/P) ratio. The data were analyzed using ANOVA and different among treatments means examined by Duncan Multiple Range Test (DMRT).<strong></strong></p><p class="MDPI17abstract"><strong>Results: </strong>The result showed that supplementation of jengkol peel powder at 6% substrate increased (P&lt;0.05) total and cellulolytic bacteria population. Jengkol peel powder supplementation as much as 4 and 6% decreased (P&lt;0.05) protozoa population, methane production, acetate proportion and A/P ratio. Supplementation of jengkol peel powder at 2% increased (P&lt;0.05) propionate production.<strong></strong></p><p class="MDPI17abstract"><strong>Conclusions: </strong>In conclusion, fermented oil palm fronds can be substituted with jengkol peel powder at 2% substrate that did not disturb rumen microbes profile, decreased methane production, acetate proportion, A/P ratio and increased propionate production on <em>in vitro</em>.<strong></strong></p>


2017 ◽  
Vol 1 (2) ◽  
pp. 33
Author(s):  
Anggun Anggun ◽  
Supriyono Supriyono ◽  
Jauhari Syamsiyah

<p>Arrowroot  able  to  be  alternative  food  because  it  has  high  enough  carbohydrate  content.  The  increase  of productivity of arrowroot can be reached by using plant spacing and fertilization. This study aims to determine the effect of plant spacing and fertilization, also their interaction on growth and yield of arrowroot (Maranta arundinacea L.). This research was held from March to November 2016 in the experimental land, Faculty of Agriculture, Sebelas Maret University. This research arranged in Completely Randomized Block Design (CRBD) consisted of 2 factors; dosage of N,P,K fertilizer (with 3 levels) and plant spacing (with 2 levels). Each treatment was repeated 4 times. The data obtained analyzed with analysis of variance and followed with Duncan Multiple Range Test at 5% level if any significant influences. The results showed that the interaction between plant spacing of 30x40 cm and urea, Sp36 and KCl 300Kg-1ha significantly increased the number of tillers and diameter of arrowroot respectively. The use of plant spacing 30x40 cm tends to increase growth and better yield. The use of 300 Kg-1ha Urea, SP36 and KCl fertilizer significantly increased plant height, while 150  Kg/ha urea,  SP36 and  KCl fertilizer significantly increased the number of tuber crops.</p>


2021 ◽  
Vol 4 (2) ◽  
pp. 122-129
Author(s):  
Enda Sabda Gentri Sembiring ◽  
Julaili Irni ◽  
Rama Riana Sitinjak ◽  
Bayu Pratomo

Introduction: This study aims to determine the growth response of Mucuna bracteata cuttings to the concentration and duration of soaking shallot extract. Materials and Methods: This study used a factorial Randomized Block Design (RBD) with two factors, namely: shallot extract concentration (P) consists of 4 levels, include P0= untreated, P1= 10 ml, P2= 20 ml, P3= 30 ml, meanwhile the soaking time (Q) consists of 4 levels, such as Q0= momentarily dipping, Q1= 15 minutes, Q2= 30 minutes, Q3= 45 minutes. The data was processed by ANOVA and if significant, then further tested with Duncan Multiple Range Test (DMRT) at the rate of 5%. Results: The concentration of shallot extract, soaking time and their interaction had a very significant effect on the survival percentage of Mucuna bracteata D.C. cuttings. The highest survival percentage of cuttings of Mucuna bracteata on the effect of shallot extract concentration and soaking time was obtained in the P0Q0, P0Q1, and P3Q2 treatments after the lid was opened at 4 weeks after planting. In the number of leaves also had significant effect in the interaction of two factors


2021 ◽  
Vol 46 (3) ◽  
pp. 278
Author(s):  
Arnandha Desya Refaldi ◽  
Prasodjo Soedomo ◽  
Muharam Muharam ◽  
Yayu Sri Rahayu

Long bean production in Indonesia continues to decline every year, this is due to land conversion and the lack of application of agricultural technology. One of the efforts to increase long bean production is by multiplying quality seeds using organic materials as nutrients needed by plants. This study aims to determine the response of the growth and production of long bean seeds to the interaction of strains, varieties with a concentration of liquid organic fertilizer. The research was conducted at the Vegetable Crops Research Institute, Lembang, West Bandung Regency from July 2020 to January 2021. The research method used was an experimental method with a Randomized Block Design (RBD) with a split-plot design consisting of 2 factors: the first factor (main plot) (A) was the variety and strain with 2 levels, a1 (Tavi canton variety) and a2 (pras-1 strain).The second factor (subplot) (B) is concentration of biopras-1 with 5 levels, b1 (0 cc/l-1 water), b2 (1 cc/l-1 water), b3 (2 cc/l-1 water), b4 (3 cc/l-1 water) and b5 (4 cc/l-1 water). The effect of the treatment was analyzed by means of variance and if the f test at 5% level was significantly different, then to find out the best treatment was continued with the DMRT (Duncan Multiple Range Test) at the 5% level. The results showed that there was an interaction between varieties, lines with various concentrations of biopras-1 liquid organic fertilizer on dry seed weight per plant, dry seed weight per plot, and 1000 seeds weight. Treatment of pras-1 lines with the concentration of liquid organic fertilizer biopras-1 4 cc / l of water was able to give the best results on dry seed weight per plant (35.31 gr), dry seed weight per plot (1.298.20 gr or 2.16 Ton/ha-1) and the weight of 1000 seeds (153.10 gr).


2018 ◽  
Vol 6 (2) ◽  
pp. 39-45
Author(s):  
Esther T. Akinyode ◽  
Justina B.O. Porbeni ◽  
David K Ojo ◽  
Olufemi O. O. Pitan ◽  
Adenike O. Olufolaji ◽  
...  

African eggplant is an important fruit and leafy vegetable in Africa. Heterosis over mid and better parents was estimated in eight crosses of eggplant involving eight pure lines in a field experiment in 2012 and 2013 cropping seasons. The experiment was laid out using a randomized complete block design with three replications. Collected data was subjected to analysis of variance and significant differences were further subjected to Duncan Multiple Range Test. The aim of this study was to identify superior hybrids that can be advanced in the eggplant breeding program for improved yield and related traits. Significant variation was observed among all traits measured for the parents and hybrids. The highest fruit number was observed in NHS10-40 and in NHS 10-71 x NHS 10-40 among the parents and hybrids respectively. The highest heterosis was recorded in the cross between NHS10-71 x NHS10-40.


2020 ◽  
Vol 9 (2) ◽  
pp. 108
Author(s):  
Wulandari Setiadarma ◽  
Dewa Gede Mayun Permana ◽  
Komang Ayu Nocianitri

This study aims to determine the effect of incubation time on milk clotting enzyme (MCE) activity produced by L. rhamnosus SKG 34 and determine the optimum incubation time to produce the highest its activity. This study used a randomized block design (RBD) with incubation time as a treatment consisting of 8 levels, that were 6 hours, 12 hours, 18 hours, 24 hours, 30 hours, 36 hours, 42 hours, and 48 hours. Data were analyzed with Variance Analysis (ANOVA) than followed by Duncan Multiple Range Test (DMRT). The analyzed were repeated 3 times resulting in 24 experimental units. The results showed that the incubation time significantly affected protease activity, MCE activity, specific protease activity and ratio of MCE to protease but did not affect the total LAB. The optimum incubation time of L. rhamnosus SKG 34 is 12 hours with total LAB 1.83 x 109 CFU/ml and protease activity 180.67 U/ml, MCE activity 595.06 SU, protease spesific activity 73.149 U/mg and ratio of MCE to protease 3.29 SU/U. Keywords : incubation time, Enzyme protease, Lactobacillus rhamnosus SKG 34, Milk clotting enzyme.


2020 ◽  
Vol 4 (2) ◽  
pp. 99-108
Author(s):  
Sakiah Sakiah ◽  
Guntoro Guntoro ◽  
Adri Moses Manullang

Paraquat is an active herbicide used to control weeds chemically. This research aim is to determine the effect of herbicide applications with active paraquat matters on the percentage of weed mortality and the number of soil microorganisms. This research used a non-factorial randomized block design with five levels of treatment, that is P0: control; P1: 5 ml of paraquat in 1 litre of water, frequency of application is once a week; P2: 10 ml of paraquat in 1 litre of water, frequency of application is once a week; P3: 10 ml of paraquat in 1 litre of water, frequency of application is once in two weeks; and P4: 5 ml of paraquat in 1 litre of water, frequency of application is once in two weeks. The results of the observations were compiled in a Variety Checklist and continued with the Duncan Multiple Range Test. The results showed that the dominant weeds in the research plot were Ageratum conyzoides, Mimosa pudica, and Paspalum commersonii.The application of 5 ml/l paraquat herbicide, the frequency of application once a week was effective in reducing weed mortality. However, the use of paraquat did not significantly affect the number of soil microorganisms


2020 ◽  
Vol 26 (1) ◽  
pp. 23
Author(s):  
Nur Eko Prasetyo ◽  
Budi Setyawan ◽  
Samijan Samijan ◽  
Nofitri Dewi Rinojati ◽  
Sumarmadji Sumarmadji

<p>The physical and chemical properties of the growing medium for <em>root trainer</em> of rubber are critical to support plant growth. This study aimed to obtain the best medium for growing rootstock rubber seedlings in the <em>root trainer</em>.  Rubber clone PR 300 seeds were germinated for 21 days, then raised in <em>root trainer</em> pots for five months. The treatments evaluated were a mixture of <em>cocopeat</em>, Rawapening peat, and manure at different ratios.  The experiment was designed in a Randomized Block Design, three replications, 30 plants per replicate, totaling 720 plants. The observed variables were N, P, K, Mg, C-organic content, pH, and cation exchange capacity of <em>cocopeat</em>, peat, and manure, as well as plant growth (height, stem diameter, wet weight, and dry weight). ANOVA used for analyzing data, followed with the <em>Duncan Multiple Range Test</em> at 5% level. The results showed that there were three best <em>root trainer</em> media for PR 300 rubber rootstock seedlings were a mixture of <em>cocopeat</em> + peat (1:1), <em>cocopeat</em> + peat + manure (1:2:1), and <em>cocopeat</em> + peat + manure (2:1:1). The growth and compactness of the roots are outstanding, so the seedlings are easily removed from the <em>root </em><em>trainer</em> pots without significant damage. Based on the initiation and cost, the best <em>root trainer</em> medium is a mixture of <em>cocopeat</em> + peat + manure (1: 2:1). It is necessary to study the use of other types of peat soils apart from Rawapening. Also, it needs to be observed further plant growth after inoculation with the stem and after planting in the field.</p><p><strong>Keywords </strong>: Planting medium, Rawapening peat, rubber clone PR 300.<em></em></p><p><em><br /></em></p><p><strong>Abstrak<br /></strong></p><p>Sifat fisik dan kimia media tanam di dalam wadah <em>root trainer</em> bibit karet sangat penting untuk mendukung pertumbuhan tanaman. Penelitian ini bertujuan mendapatkan media terbaik untuk pertumbuhan bibit batang bawah karet di dalam <em>root trainer</em>.  Benih klon karet PR 300 dikecambahkan selama 21 hari pada bedeng pengecambahan kemudian dipindah-tanam dan dibesarkan selama 5 bulan dalam pot <em>root trainer</em>. Perlakuannya yaitu campuran <em>cocopeat</em>, gambut Rawapening, dan pupuk kandang (pukan) pada berbagai ratio, dan kontrol. Penelitian dirancang secara Acak Kelompok, tiga ulangan, masing-masing 30 tanaman, sehingga totalnya 720 tanaman. Variabel yang diamati yaitu kandungan N, P, K, Mg, C-organik, pH, dan kapasitas tukar kation dari <em>cocopeat</em>, gambut, dan pupuk kandang, serta pertumbuhan tanaman (tinggi, diameter batang, bobot segar, dan bobot kering). Data dianalisis dengan ANOVA, kemudian <em>Duncan Multiple Range Test</em> pada taraf 5%. Hasil penelitian menunjukkan bahwa ada tiga macam media <em>root trainer</em> terbaik untuk bibit batang bawah karet klon PR 300 yaitu campuran <em>cocopeat </em>+ gambut (1:1), <em>cocopeat </em>+ gambut + pukan (1:2:1), dan <em>cocopeat </em>+ gambut + pukan (2:1:1). Pertumbuhan dan kekompakan akar sangat baik sehingga bibit mudah dilepas dari <em>root tainer</em> tanpa kerusakan berarti. Berdasarkan kepraktisan dan biaya maka media <em>root trainer</em> terbaik adalah campuran media <em>cocopeat </em>+ gambut + pukan (1:2:1). Perlu dilakukan kajian mengenai pemanfaatan tanah gambut jenis lainnya selain dari Rawapening. Selain itu, juga perlu diamati lebih lanjut pertumbuhan tanaman setelah diokulasi dengan batang atas maupun setelah ditanam di lapangan.</p><p><strong>Kata kunci </strong>: Gambut Rawapening, klon karet PR 300, media tanam.</p>


2015 ◽  
Vol 6 (1) ◽  
pp. 54 ◽  
Author(s):  
Lefin Kafindra ◽  
Nurul Khumaida ◽  
Sintho Wahyuning Ardie

<p>ABSTRACT</p><p>Kaempferia  parviflora  is a medicinal plant  that contains  secondary metabolites  and  effective in alleviating some  diseases.  Rapid multiplication of K.  parviflora  through  its rhizome is hampered by the long period needed to produce the rhizome. The objectives of this research were to obtain the best sucrose  concentration  and  to  determine  the  best  BAP  concentration  in the in vitro  microrhizome induction of  K.  parviflora.  The experiment was arranged in a randomized complete block design with two factors and three replications.  The first factor was BAP concentration, consisted of three levels i.e. 0, 2, and 4 mg L1. The second factor was sucrose concentration, consisted of four levels  i.e.  0,  30,  60,  and  90  g  L1.  Data  obtained  were  analyzed by  F-test,  followed  by  DMRT (Duncan  Multiple Range Test) at 5% significant level.  Sucrose  concentration  significantly affected the  number of shoots  and plantlets  fresh  weight.  The results showed that  K.  parviflora  could form micro  rhizome  in  vitro,  as  indicated  by  similar  anatomical  structure compared  to  the  rhizome produced in vivo. K. parviflora cultured in MS medium containing 90 g L1sucrose without BAP had the highest number of micro rhizome at 8 weeks after culture.</p><p>Keywords: health, black galingale, traditional medicine</p><p> </p><p>ABSTRAK</p><p>Kaempferia  parviflora  merupakan  tanaman  obat  yang  mengandung metabolit  sekunder  dan berkhasiat  untuk  mengobati  berbagai  macam penyakit.  Perbanyakan  K.  parviflora  menggunakan rimpang terkendala oleh lamanya waktu untuk memproduksi rimpang di lapang, yaitu mencapai 8 bulan setelah  tanam.  Tujuan  penelitian  ini  ialah  memperoleh  konsentrasi sukrosa terbaik  dan konsentrasi  BAP  terbaik  dalam  induksi  rimpang  mikro  K. parviflora.  Percobaan  ini  disusun berdasarkan  rancangan  kelompok  lengkap teracak  dengan  dua  faktor  dan  tiga  ulangan.  Faktor pertama  adalah konsentrasi  BAP  dengan  3  taraf,  yaitu  0,  2,  dan  4  mg  L1.  Faktor kedua adalah konsentrasi  sukrosa  dengan  4  taraf,  yaitu  0,  30,  60,  dan  90  g L1.  Data  yang  diperoleh  dianalisis menggunakan  uji  F  dan  apabila berpengaruh  nyata,  maka  dilanjutkan  dengan  uji  lanjut  DMRT (Duncan Multiple Range Test) pada taraf α= 5 %. Hasil penelitian menunjukkan bahwa K. parviflora dapat membentuk rimpang mikro  in vitro. Hal tersebut ditunjukkan oleh struktur anatomi rimpang mikro  yang  serupa  dengan rimpang  in  vivo.  K. parviflora  yang  dikulturkan  pada  media  MS yang mengandung 90 g L1sukrosa tanpa BAP memiliki jumlah rimpang mikro tertinggi pada 8 MSP.</p><p>Kata kunci: kesehatan, kencur hitam, obat tradisional</p>


Jurnal Agro ◽  
10.15575/438 ◽  
2015 ◽  
Vol 2 (2) ◽  
pp. 50-57 ◽  
Author(s):  
Muhamad Fajar Ramadhan ◽  
Cecep Hidayat ◽  
Sofiya Hasani

Penelitian yang bertujuan untuk mengetahui pengaruh bahan organik (kompos gamal, dan asam humat) serta FMA (Glomus sp. + Gigaspora sp.+ Aclauspora sp) terhadap pertumbuhan serta hasil tanaman cabai pada tanah pasca galian C telah dilaksanakan di Gapoktan Simpay Tampomas, Sumedang sejak bulan Februari sampai dengan Juli 2014. Metode yang digunakan merupakan metode eksperimental berupa Rancangan Acak Kelompok (RAK),  dengan 8 perlakuan dan 4 kali ulang: a0 = kontrol, a1 = kompos gamal 5 t ha-1, a2 = kompos gamal 10 t ha-1, a3 = aplikasi FMA, a4 = asam humat, a5 = kompos gamal 5 t ha-1 + FMA , a6 =  kompos gamal 10 t ha-1 + FMA, dan a7 = aplikasi asam humat + FMA. Pengujian lanjut dilakukan dengan Duncan Multiple Range Test (DMRT). Hasil penelitian menunjukkan bahwa aplikasi kompos gamal, asam humat, dan FMA tidak berpengaruh nyata terhadap diameter batang dan bobot segar buah, tetapi berbeda nyata terhadap tinggi tanaman. Pengaplikasian bahan organik hingga 10 t ha-1, asam humat, dan FMA belum berpengaruh nyata terhadap perbaikan kesuburan tanah pasca galian C (tambang pasir), karena kondisi tanah pasca galian C yang didominasi 75% pasir dan 50% batuan. The study which aimed to determine the effect of organic matter (gliricidia compost, and humic acid) also AMF (Glomus sp. + Gigaspora sp. + Aclauspora sp.) on growth and yield of chili plants on post-excavation soil C had been held in Gapoktan Simpay Tampomas, Sumedang from February to July 2014. The method was an experimental method a randomized block design (RBD), with 8 treatments and repeated 4 times: a0 = control, a1 = gliricidia compost 5 t ha-1, a2 = gliricidia compost 10 t ha-1, a3 = AMF, a4 = humic acid, a5 = gliricidia compost 5 tons ha-1 + AMF, a6 = gliricidia compost 10 tons ha-1 + AMF, and a7 = humic acid + AMF. Further testing was done by Duncan Multiple Range Test (DMRT). The results showed that the application of gliricidia compost, humic acid, and AMF did not significantly affect stem diameter, and fresh weight of fruit, but significant on stem height. Application of organic matter up to 10 tons ha-1, humic acid, and AMF had not significantly affect fertility improvement of post-excavation soil C (sand mining), because the soil C dominated by 75% sand and 50% rock.


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