scholarly journals Induksi Rimpang Mikro Kaempferia parviflora secara In Vitro dengan Penambahan BAP dan Sukrosa

2015 ◽  
Vol 6 (1) ◽  
pp. 54 ◽  
Author(s):  
Lefin Kafindra ◽  
Nurul Khumaida ◽  
Sintho Wahyuning Ardie
Keyword(s):  
Sucrose Concentration ◽  
Block Design ◽  
Duncan Multiple Range Test ◽  
Kaempferia Parviflora ◽  
L1 Data ◽  
Two Factors ◽  
Four Levels ◽  
Range Test

<p>ABSTRACT</p><p>Kaempferia  parviflora  is a medicinal plant  that contains  secondary metabolites  and  effective in alleviating some  diseases.  Rapid multiplication of K.  parviflora  through  its rhizome is hampered by the long period needed to produce the rhizome. The objectives of this research were to obtain the best sucrose  concentration  and  to  determine  the  best  BAP  concentration  in the in vitro  microrhizome induction of  K.  parviflora.  The experiment was arranged in a randomized complete block design with two factors and three replications.  The first factor was BAP concentration, consisted of three levels i.e. 0, 2, and 4 mg L1. The second factor was sucrose concentration, consisted of four levels  i.e.  0,  30,  60,  and  90  g  L1.  Data  obtained  were  analyzed by  F-test,  followed  by  DMRT (Duncan  Multiple Range Test) at 5% significant level.  Sucrose  concentration  significantly affected the  number of shoots  and plantlets  fresh  weight.  The results showed that  K.  parviflora  could form micro  rhizome  in  vitro,  as  indicated  by  similar  anatomical  structure compared  to  the  rhizome produced in vivo. K. parviflora cultured in MS medium containing 90 g L1sucrose without BAP had the highest number of micro rhizome at 8 weeks after culture.</p><p>Keywords: health, black galingale, traditional medicine</p><p> </p><p>ABSTRAK</p><p>Kaempferia  parviflora  merupakan  tanaman  obat  yang  mengandung metabolit  sekunder  dan berkhasiat  untuk  mengobati  berbagai  macam penyakit.  Perbanyakan  K.  parviflora  menggunakan rimpang terkendala oleh lamanya waktu untuk memproduksi rimpang di lapang, yaitu mencapai 8 bulan setelah  tanam.  Tujuan  penelitian  ini  ialah  memperoleh  konsentrasi sukrosa terbaik  dan konsentrasi  BAP  terbaik  dalam  induksi  rimpang  mikro  K. parviflora.  Percobaan  ini  disusun berdasarkan  rancangan  kelompok  lengkap teracak  dengan  dua  faktor  dan  tiga  ulangan.  Faktor pertama  adalah konsentrasi  BAP  dengan  3  taraf,  yaitu  0,  2,  dan  4  mg  L1.  Faktor kedua adalah konsentrasi  sukrosa  dengan  4  taraf,  yaitu  0,  30,  60,  dan  90  g L1.  Data  yang  diperoleh  dianalisis menggunakan  uji  F  dan  apabila berpengaruh  nyata,  maka  dilanjutkan  dengan  uji  lanjut  DMRT (Duncan Multiple Range Test) pada taraf α= 5 %. Hasil penelitian menunjukkan bahwa K. parviflora dapat membentuk rimpang mikro  in vitro. Hal tersebut ditunjukkan oleh struktur anatomi rimpang mikro  yang  serupa  dengan rimpang  in  vivo.  K. parviflora  yang  dikulturkan  pada  media  MS yang mengandung 90 g L1sukrosa tanpa BAP memiliki jumlah rimpang mikro tertinggi pada 8 MSP.</p><p>Kata kunci: kesehatan, kencur hitam, obat tradisional</p>

scholarly journals Growth Response of Mucuna Cuttings to Concentration and Duration of Soaking Shallot Extract

2021 ◽  
Vol 4 (2) ◽  
pp. 122-129
Author(s):  
Enda Sabda Gentri Sembiring ◽  
Julaili Irni ◽  
Rama Riana Sitinjak ◽  
Bayu Pratomo
Keyword(s):  
Growth Response ◽  
Block Design ◽  
Duncan Multiple Range Test ◽  
Soaking Time ◽  
Extract Concentration ◽  
Survival Percentage ◽  
Randomized Block Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Range Test

Introduction: This study aims to determine the growth response of Mucuna bracteata cuttings to the concentration and duration of soaking shallot extract. Materials and Methods: This study used a factorial Randomized Block Design (RBD) with two factors, namely: shallot extract concentration (P) consists of 4 levels, include P0= untreated, P1= 10 ml, P2= 20 ml, P3= 30 ml, meanwhile the soaking time (Q) consists of 4 levels, such as Q0= momentarily dipping, Q1= 15 minutes, Q2= 30 minutes, Q3= 45 minutes. The data was processed by ANOVA and if significant, then further tested with Duncan Multiple Range Test (DMRT) at the rate of 5%. Results: The concentration of shallot extract, soaking time and their interaction had a very significant effect on the survival percentage of Mucuna bracteata D.C. cuttings. The highest survival percentage of cuttings of Mucuna bracteata on the effect of shallot extract concentration and soaking time was obtained in the P0Q0, P0Q1, and P3Q2 treatments after the lid was opened at 4 weeks after planting. In the number of leaves also had significant effect in the interaction of two factors

scholarly journals Supplementation of Jengkol Peel on VFA Molar Proportion, Methane Production, and Hydrogen Balance in Vitro

2020 ◽  
Vol 22 (2) ◽  
pp. 150
Author(s):  
N. Hidayah ◽  
Nurhaita Nurhaita ◽  
R. Zurina
Keyword(s):  
Hydrogen Production ◽  
Analysis Of Variance ◽  
Methane Production ◽  
Block Design ◽  
H2 Production ◽  
Duncan Multiple Range Test ◽  
Molar Proportion ◽  
Randomized Block Design ◽  
Range Test

This research was designed to study about effects of supplementation jengkol (Archidendron jiringa) peel powder on VFA molar proportion, production of methane, and hydrogen balance in vitro. The experiment used randomized block design with 4 treatments (0%, 2%, 4%, 6%) and 4 replications. The research observed of VFA molar proportion, production of methane, and hydrogen balance. Data were analyzed using Analysis of Variance (ANOVA), and the Duncan Multiple Range Test examined the differences among treatment means. The results showed that the supplementation of jengkol (A. jiringa) peel powder until 6% did not affect (P>0.05) VFA molar proportion but the treatment without supplementation of jengkol (A. jiringa) peel powder had the highest (P<0.05) methane and H2 production. It was concluded that the supplementation of jengkol (A. jiringa) peel powder until 6% decreased methane and hydrogen production but did not affect on VFA molar proportion.

scholarly journals Pengaruh Abu Vulkanik Kelud dan Pupuk Kandang terhadap Ketersediaan dan Serapan Kalium pada Jagung di Tanah Alfisol

2016 ◽  
Vol 18 (1) ◽  
pp. 18
Author(s):  
Suntoro Suntoro ◽  
Sudadi Sudadi ◽  
Hery Widijanto ◽  
Galuh Novikah Widy Utami
Keyword(s):  
Crop Production ◽  
Volcanic Ash ◽  
Chemistry Laboratory ◽  
Duncan Multiple Range Test ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Four Levels ◽  
Total Potassium ◽  
Range Test

<p>Kelud volcanic ash has high source of minerals, which is potassium. This study aims to determine the effect and find the best composition of Kelud volcanic ash and manure to the availability and corn uptake of potassium in the Alfisols.This research is expected to provide information on the composition of volcanic ash Kelud and proper manure for agriculture. This research was conducted in the greenhouse, in the Laboratory of Ecology and Management of Crop Production, and soil chemistry laboratory of the Faculty of Agriculture, Sebelas Maret University in June 2014 - January 2015. Research using completely randomized design which consists of two factors, there are the thickness of Kelud volcanic ash which consists of four levels A0 (0 cm), A1 (2 cm), A2 (4 cm), and A3 (6 cm) and the dose of manure were P0 (0 ton/ha), P1 (2,5 ton/ha), and P2 (5 ton/ha). Each combination treatment was repeated 3 times. The data were then analyzed using the F test and continued with Duncan Multiple Range Test Test (DMRT) level of 95%. The results showed that the interaction between the two treatments were no significant different. Availability Potassium gradually decreased with an increase in the dose of volcanic ash Kelud, allegedly because of the low value of the total potassium Kelud volcanic ash. The results of measurements of plant potassium uptake increased with increasing dose Kelud volcanic ash and manure.</p>

scholarly journals IN VITRO SHOOT INDUCTION OF GARUT (Maranta arundinacea) WITH THE ADDITION OF 2,4-D BENZYL ADENIN

Agrivet ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 43
Author(s):  
Endah Wahyurini
Keyword(s):  
Design Method ◽  
Shoot Induction ◽  
Food Crop ◽  
Duncan Multiple Range Test ◽  
Benzyl Adenine ◽  
In Vitro Techniques ◽  
Two Factors ◽  
Treatment Concentration ◽  
Range Test

Arrowroot (Maranta arundinacea L.) is a type of forest tuber plant that potentially developed as a local food crop. Arrowroot tubers can be processed as chips and starches. The difficulty of getting superior varieties and seeds in relatively large quantities and uniform can be overcome by in vitro techniques. The success of tissue culture depends on the planting media, PGR, vitamins and plant genetics. The purpose of this study was to determine the effect of giving 2.4 D and BA on the growth of arrowroot explants, as well as getting the proper concentrations of 2.4 D and BA to stimulate the growth of arrowroot tubers in vitro. The study wa conducted in a laboratory with two factors Complete Random Design Method. The first factor is the 2.4 D concentration which consists of three levels, namely: 0.5 mg / L (D1), 1 mg / L (D2) and 1.5 mg / L (D3). The second factor is the concentration of BA consisting of three levels, namely: 1 mg / L (B1), 2 mg / L (B2) and 3 mg / L (B3). Variance analysis were done at 5% level. To find out there is a real difference between treatments, Duncan Multiple Range Test (DMRT) at 5% level were conducted. The results showed that the 2.4 D treatment concentration of 1 mg / L produced a greater percentage of shoot life than other treatments. Giving 2.4 D concentrations of 1 mg / L and BA 2 mg / L stimulated quicker emergence of shoots and higher shoot lengths compared to other treatments.Keyword: 2,4 D, Benzyl adenine, Maranta arundinacea, in vitro

In Vitro Germination of Eucalyptus Pollen: Response to Variation in Boric Acid and Sucrose

1989 ◽  
Vol 37 (5) ◽  
pp. 429 ◽  
Author(s):  
BM Potts ◽  
JB Marsden-Smedley
Keyword(s):  
Pollen Tube ◽  
Boric Acid ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Sucrose Concentration ◽  
Response Surfaces ◽  
Tube Growth ◽  
Pollen Competition

The effect of boric acid (0-450 ppm) and sucrose (0-40%) on pollen germination and pollen tube growth in Eucalyptus globulus, E. morrisbyi, E. ovata and E. tirnigera was examined in vitro. Over the con- centrations tested, sucrose had by far the largest effect upon both pollen germination and tube lengths. The optimum sucrose concentration for pollen germination (30%) and pollen tube growth (20%) differed markedly with very little (<lo%) germination occurring in the absence of sucrose. The interaction of sucrose and boric acid was significant. However, in general both pollen germination and pollen tube growth were increased by the addition of up to 100 ppm boric acid, but above this level the response plateauxed. The four species differed significantly in their pattern of response to both boric acid and sucrose and the predicted optima derived from analysis of response surfaces differed between species. The predicted sucrose concentration for optimal germination and growth of E. urnigera pollen was consistently less than the other species and in terms of the optimal level of boric acid for pollen tube growth species can be ranked in the order E. globulus > E. ovata > E. morrisbyi = E. urnigera. Pollen germination and tube growth of all four species on a medium comprising 20% sucrose and 200 ppm boric acid would not differ significantly from the observed maximum response of each species and this could suffice as a generalised medium. However, if only percentage germination is to be assessed 30% sucrose would be preferable. It is argued that subtle interspecific differences in optimal in vitro con- ditions for pollen germination and pollen tube growth are likely to reflect differences in pollen physiology which in vivo may have important implications for the success of hybridisation where pollen competition occurs.

scholarly journals Response of growth and yield of two soybean varieties to seed pre-treatment applications

2021 ◽  
Vol 883 (1) ◽  
pp. 012052
Author(s):  
N Herawati ◽  
A R Aisah ◽  
I Mardian ◽  
B N Hidayah ◽  
B T R Erawati
Keyword(s):  
Plant Height ◽  
Seed Treatment ◽  
Seed Quality ◽  
Block Design ◽  
Growth And Yield ◽  
Agronomic Crops ◽  
Randomized Block Design ◽  
Two Factors ◽  
Pre Treatment ◽  
Four Levels

Abstract Plant growth is influenced by seed quality. Seeds need to be treated to prevent pest and disease disorders or to increase seed germination. Soybean planting was carried out in this study by the treatment of varieties and seed pre-treatment application before planting. The study aimed to measure the growth and yield of soybeans by varieties and seed treatment before planting, carried out in the Village of Nggembe, District of Bolo, Bima Regency. The study used factorial randomized block design with two factors. The first factor was soybean varieties consisting of two levels, namely Devon and Dena varieties, and the second factor was the application of pre-planting seed treatments consisting of four levels namely Cruiser, Marshall, Agrisoy, and without seed treatment, each treatment was repeated three times. Observations were made on the performance of agronomic crops and soybean yields. The results showed that the treatment of soybean varieties and the application of seed treatment had a significant influence on plant height and soybean productivity, and both treatments had interactions on parameters of plant height, number of filled pods, weight of 100 seeds, and soybean productivity with the best results successively produced by a combination of Devon-Control, Devon-Marshall, Devon-Agrisoy, and Dena-Cruiser.

scholarly journals COMPARISON OF YIELD AMONG DIFFERENT HIGH YIELDING VARIETIES OF WETLAND RICE

Agric ◽  
2021 ◽  
Vol 33 (1) ◽  
pp. 57-66
Author(s):  
Kiki Kusyaeri Hamdani ◽  
Yati Haryati
Keyword(s):  
Yield Components ◽  
Block Design ◽  
Yield Component ◽  
Yield Potential ◽  
Duncan Multiple Range Test ◽  
Rice Varieties ◽  
Randomized Complete Block Design ◽  
High Yielding Varieties ◽  
Range Test ◽  
Better Than

New superior varieties (VUB) are a reliable technological innovation to increase rice productivity. This study aims to determine the yield potential of some lowland rice VUB. The assessment was carried out on land owned by a member of the Sumber Rejeki Farmer Group, Cintaratu Village, Lakbok District, Ciamis Regency at Dry Season II in June-September 2020. The study used a randomized complete block design (RCBD) with six varieties of treatment and was repeated ten times. The varieties tested were Inpari 32, Inpari 42, Padjadjaran, Cakrabuana, Inpari IR Nutrizinc, and Siliwangi varieties. The variables observed included the growth component, yield component, and yield component. Data were analyzed using the F test followedby the Duncan Multiple Range Test at the Q=5% level. In addition, a correlation test was conducted between the growth components, yield components, and yields. The results of the study indicated that the new superior rice varieties studied had different performance in growth, number of tillers, yield, and yield components. Inpari 42 variety produced the highest productivity, namely 6.88 ton ha-1 which was supported by the number of grains per panicle, percentage of filled grains per panicle, and percentage of empty grain per panicle which were better than other varieties. Plant height and number of grains per panicle were positively correlated with yield.

scholarly journals PENGARUH KONSENTRASI PUPUK ORGANIK CAIR SERTA VARIETAS DAN GALUR TERHADAP PERTUMBUHAN DAN PRODUKSI BENIH TANAMAN KACANG PANJANG (Vigna sinensis (L.) Savi ex Hassk.)

2021 ◽  
Vol 46 (3) ◽  
pp. 278
Author(s):  
Arnandha Desya Refaldi ◽  
Prasodjo Soedomo ◽  
Muharam Muharam ◽  
Yayu Sri Rahayu
Keyword(s):  
Seed Weight ◽  
Block Design ◽  
Organic Fertilizer ◽  
Agricultural Technology ◽  
Vegetable Crops ◽  
Duncan Multiple Range Test ◽  
Randomized Block Design ◽  
Main Plot ◽  
Range Test ◽  
Plot Design

Long bean production in Indonesia continues to decline every year, this is due to land conversion and the lack of application of agricultural technology. One of the efforts to increase long bean production is by multiplying quality seeds using organic materials as nutrients needed by plants. This study aims to determine the response of the growth and production of long bean seeds to the interaction of strains, varieties with a concentration of liquid organic fertilizer. The research was conducted at the Vegetable Crops Research Institute, Lembang, West Bandung Regency from July 2020 to January 2021. The research method used was an experimental method with a Randomized Block Design (RBD) with a split-plot design consisting of 2 factors: the first factor (main plot) (A) was the variety and strain with 2 levels, a1 (Tavi canton variety) and a2 (pras-1 strain).The second factor (subplot) (B) is concentration of biopras-1 with 5 levels, b1 (0 cc/l-1 water), b2 (1 cc/l-1 water), b3 (2 cc/l-1 water), b4 (3 cc/l-1 water) and b5 (4 cc/l-1 water). The effect of the treatment was analyzed by means of variance and if the f test at 5% level was significantly different, then to find out the best treatment was continued with the DMRT (Duncan Multiple Range Test) at the 5% level. The results showed that there was an interaction between varieties, lines with various concentrations of biopras-1 liquid organic fertilizer on dry seed weight per plant, dry seed weight per plot, and 1000 seeds weight. Treatment of pras-1 lines with the concentration of liquid organic fertilizer biopras-1 4 cc / l of water was able to give the best results on dry seed weight per plant (35.31 gr), dry seed weight per plot (1.298.20 gr or 2.16 Ton/ha-1) and the weight of 1000 seeds (153.10 gr).

scholarly journals THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Agrivet ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 39
Author(s):  
Salma Nabila ◽  
Endah Budi Irawati ◽  
Rina Srilestari
Keyword(s):  
Water Concentration ◽  
Coconut Water ◽  
Fresh Weight ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Range Test ◽  
Growing Shoot

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety  of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on  fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

scholarly journals PRODUCTION AND FUNGICIDAL ACTIVITY ASSESMENT OF WOOD-WASTE LIQUID SMOKE

2020 ◽  
Vol 8 (10) ◽  
pp. 285-291
Author(s):  
Budy Rahmat ◽  
Dedi Natawijaya ◽  
Endang Surahman
Keyword(s):  
Plant Pathogens ◽  
Pathogenic Fungus ◽  
Block Design ◽  
Control Plant ◽  
Wood Waste ◽  
In Vitro Test ◽  
In Vivo Test ◽  
Liquid Smoke

Liquid smoke is known to contain compounds that can control plant disease pathogens. This study aims to produce wood-waste liquid smoke and determine its effectiveness as a fungicide on plant pathogens. This research was conducted in two experimental stages, namely: (i) in vitro test as a preliminary test of the effectiveness of teak waste liquid smoke at concentrations of 0, 0.5, 1, 1.5, 2, and 2.5%; and (ii) in vivo test was arranged in randomized block design consisting of seven levels of liquid smoke concentration, namely 0, 1, 2, 3, 4, 5, and 6%, each of which was repeated four times. The results showed that the pyrolysis of 1 kg of wood waste was produced with the proportions of liquid smoke, charcoal and tar, respectively: 312 mL, 31 g, 367 g and the uncondensed gases. Treatment of liquid smoke in the in vivo test showed that a concentration of 1 to 2.5% liquid smoke was able to suppress the growth of the pathogenic fungus Sclerotium rolfsii 100%. The treatment of liquid smoke in the in vivo test showed an effect on inhibition of the growth diameter of fungal colonies, suppressing the disease occurance, and suppressing the lesion diameter.

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