scholarly journals Penetapan Kadar Fenolik dan Flavonoid Hasil Ultrasonic Assisted Extraction Daun Binahong (Anredera cordifolia [Ten] Steenis)

2020 ◽  
Vol 11 (1) ◽  
pp. 16-23
Author(s):  
Landyyun Rahmawan Sjahid ◽  
Amelia Aqshari ◽  
Sediarso Sediarso

Anredera cordifolia [Ten] Steenis has been proven to have pharmacological activity that is thought to be influenced by the presence of phenolic compounds and flavonoids. However, both compounds are found in small concentrations through conventional extraction (maceration, decoction, soxhlet, and reflux). This study aims to prove that the ultrasonic assisted extraction method can obtain higher concentrations of phenolic and flavonoid compounds. Dried binahong leaves were extracted using ultrasonic assisted extraction with 70% ethanol. Phenolic levels were determined using Folin-Ciocalteu, while flavonoid levels were determined using the colorimetric aluminum method. The results showed binahong leaves ethanolic extract had phenolic levels of 10.16 % GAE and flavonoid levels of 10.58 % QE. These results are much higher than the results obtained using conventional extraction so that ultrasonic assisted extraction can be recommended to maximize the extraction of phenolic and flavonoid levels from the leaves of Binahong (Anredera cordifolia [Ten] Steenis). 

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1759 ◽  
Author(s):  
Francisco J. Barba ◽  
Cristina Alcántara ◽  
Radhia Abdelkebir ◽  
Christine Bäuerl ◽  
Gaspar Pérez-Martínez ◽  
...  

The paper presents experimental results concerning the ultrasonically-assisted extraction of bioactive compounds from Erodium glaucophyllum roots. A comparison with conventional methodology is presented, and thereby the phytochemical composition and the antioxidant and anti-inflammatory activities of extracts are evaluated. The phenolic profile of Erodium extracts was analyzed by TOF–LC–MS–MS. The identification of phenolic compounds revealed that the major component was (+)-gallocatechin in the aqueous extracts obtained for the different extraction methodologies. The highest quantity of phenolic compounds and antioxidant capacity was found in the hydroethanolic extract obtained by conventional extraction (29.22–25.50 mg GAE/g DM; 21.174 mM Trolox equivalent). The highest content of carotenoids, varying from 0.035 to 0.114 mg/g dry matter, was reached by ultrasonic-assisted extraction. Furthermore, Erodium extracts showed a potent inhibition of the inflammatory reaction by means of the inhibition of tumor necrosis factor-alpha (TNF-α). The extracts obtained when ultrasound extraction was combined with ethanol:water (50:50, v/v) presented the greatest inhibition (92%).


2018 ◽  
Vol 48 (6) ◽  
Author(s):  
Caroline Pagnossim Boeira ◽  
Natiéli Piovesan ◽  
Marcela Bromberger Soquetta ◽  
Déborah Cristina Barcelos Flores ◽  
Bruna Nichelle Lucas ◽  
...  

ABSTRACT: The aim of this study was to evaluate the effect of extraction conditions on bioactive compounds, as well as on antioxidant activity, and the antimicrobial activity of the extracts with the highest antioxidant characteristics. The extracts were obtained by conventional method and ultrasound-assisted extraction at various temperatures (20°C, 40°C and 60°C). Total phenolics, total flavonoids, antimicrobial activity and antioxidant activity were quantified by the methods of DPPH, FRAP, and ORAC, respectively. The conventional extraction method and ultrasound method influenced the phenolic content at all the tested temperatures. Flavonoids were not influenced by extraction methods. The antioxidant activity (DPPH) was highest in the ultrasonic method at temperatures of 40°C and 60°C; however, in the case of the FRAP method the best results were for the conventional extraction method. The conventional and ultrasonic methods did not influence the IC50 at temperatures of 20°C and 40°C, but using ORAC the antioxidant activity was influenced by the methods at all temperatures. The extract obtained at 60°C by the ultrasound method had high antimicrobial action in relation to the strains of Salmonella sp., Escherichia coli, and Staphylococcus aureus. Extraction ultrasonic-assisted can be considered adequate to obtain extracts of marcela, which are rich in bioactive compounds with high antioxidant activity.


LWT ◽  
2020 ◽  
Vol 134 ◽  
pp. 110243
Author(s):  
Maryam Iftikhar ◽  
Huijuan Zhang ◽  
Asra Iftikhar ◽  
Ali Raza ◽  
Nabila Begum ◽  
...  

Chemosphere ◽  
2006 ◽  
Vol 63 (3) ◽  
pp. 411-420 ◽  
Author(s):  
T.G. Kazi ◽  
M.K. Jamali ◽  
A. Siddiqui ◽  
G.H. Kazi ◽  
M.B. Arain ◽  
...  

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