scholarly journals Evaluation of antibacterial activity of essential oil from Algerian Pistacia lentiscus resin

2017 ◽  
Vol 1 ◽  
pp. 13
Author(s):  
G. Bachir Raho
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Diffusion Method ◽  
Pistacia Lentiscus ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Agar Disc ◽  
In Vitro Antibacterial Activity ◽  
Microbial Growth Inhibition

The purpose of this research was to evaluate the in vitro antibacterial activity of the essential oils from the resin of Pistacia lentiscus against Staphylococcus aureus (Gram–positive bacteria) and Escherichia coli (Gram–negative bacteria). The agar disc diffusion method was used for microbial growth inhibition at various dilutions of the oils. Results showed that the tested essential oils possess antibacterial activity against S. aureus but inactive on E. coli. These results may have significant implications for the future development of resin oils of P. lentiscus as an antimicrobial agent for the treatment of the infections caused by S. aureus. 

scholarly journals Evaluation of the in vitro antibacterial activity of some essential oils and their blends against Staphylococcus spp. isolated from episodes of sheep mastitis

2021 ◽  
Author(s):  
Filippo Fratini ◽  
Margherita Giusti ◽  
Simone Mancini ◽  
Francesca Pisseri ◽  
Basma Najar ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Diffusion Method ◽  
Minimal Bactericidal Concentration ◽  
Coagulase Negative Staphylococci ◽  
Disk Diffusion Method ◽  
In Vitro Antibacterial Activity ◽  
High Concentrations ◽  
Thymus Serpyllum

AbstractStaphylococcus aureus and coagulase-negative staphylococci are among the major causes of mastitis in sheep. The main goal of this research was to determine the in vitro antibacterial activity of several essential oils (EOs, n 30), then five of them were chosen and tested alone and in blends against staphylococci isolates. Five bacteria were isolated from episodes of ovine mastitis (two S. aureus and three S. xylosus). Biochemical and molecular methods were employed to identify the isolates and disk diffusion method was performed to determine their antimicrobial-resistance profile. The relative percentage of the main constituents in the tested essential oils and their blends was detected by GC-EIMS analysis. Antibacterial and bactericidal effectiveness of essential oils and blends were evaluated through minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). All of them showed sensitivity to the used antimicrobials. The EOs with the highest antibacterial activity were those belonging to the Lamiaceae family characterized by high concentrations of thymol, carvacrol and its precursor p-cymene, together with cinnamon EO, rich in cinnamaldehyde. In terms of both MIC and MBC values, the blend composed by Thymus capitatus EO 40%, Cinnamomum zeylanicum EO 20%, Thymus serpyllum EO 20% and Satureja montana EO 20% was found to be the most effective against all the isolates. Some essential oils appear to represent, at least in vitro, a valid tool against ovine mastitis pathogens. Some blends showed a remarkable effectiveness than the single oils, highlighting a synergistic effect in relation to the phytocomplex.

scholarly journals EVALUATION OF ANTIBACTERIAL ACTIVITY OF ESSENTIAL OIL OF CINNAMOMUM ZEYLANICUM, EUGENIA CARYOPHYLLATA, AND ROSMARINUS OFFICINALIS AGAINST STREPTOCOCCUS ORALIS

2017 ◽  
Vol 10 (5) ◽  
pp. 410 ◽  
Author(s):  
Samira Hsaine ◽  
Reda Charof ◽  
Khadija Ounine
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Diffusion Method ◽  
Cinnamomum Zeylanicum ◽  
Streptococcus Oralis ◽  
In Vitro Antibacterial Activity ◽  
Broth Dilution

Objective: Streptococcus oralis plays an important role in the biofilm formation of dental plaque and the occurrence of periodontal disease. Thepresent study was conducted to evaluate in vitro antibacterial activity of three essential oils, namely, Cinnamomum zeylanicum, Eugenia caryophyllata,and Rosmarinus officinalis against S. oralis.Methods: The antibacterial activity of essential oils was investigated by diffusion method using sterile discs (or aromatograms). The minimuminhibitory concentration (MIC) of essential oils showing important antibacterial activity was measured using the broth dilution method.Results: Evaluation of the antibacterial activity of three essential oils as determined by the aromatogram technique showed that the essential oilof R. officinalis had no effect on S. oralis, while the latter was extremely sensitive to the other two essential oils, but with a higher efficiency of theessential oil of C. zeylanicum (42 mm diameter) than E. caryophyllata (20 mm diameter). Similarly, the MIC and minimum bactericidal concentration(MBC) were higher for the essential oil of C. zeylanicum than the essential oil of E. caryophyllata. The MBC/MIC ratio is of the order of 2. The essentialoils studied therefore appear to exert bactericidal activity against S. oralis.Conclusion: The findings suggest that essential oils of C. zeylanicum and E. caryophyllata may be used as an alternative to synthetic antibiotics.Keywords: Essential oil, Cinnamomum zeylanicum, Eugenia caryophyllata, Rosmarinus officinalis, Antimicrobial activity, Streptococcus oralis.

scholarly journals Synthesis and Pharmacological Evaluation of 2-(4-(3 (Substituted Phenyl) Acryloyl) Phenoxy)-N, N Diphenylacetamides

2020 ◽  
Vol 10 (5) ◽  
pp. 274-292
Author(s):  
Rohit Kumar ◽  
Sushil Kumar ◽  
Mohammad Asif Khan
Keyword(s):  
Antibacterial Activity ◽  
Schiff Bases ◽  
Biological Activities ◽  
Diffusion Method ◽  
Gram Positive ◽  
Standard Drug ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Pharmacological Evaluation

Recently a series of Schiff bases of diphenylamine derivatives have been synthesized and evaluated in vitro for their antibacterial activity against pathogenic both Gram-positive bacteria B. subtitles and Gram-negative bacteria E. coli using ciprofloxacin as standard drug at conc. of 50 μg/ml and 100 μg/ml. Literature review revels that chalcones possesses various biological activities like antimicrobial, antiviral, anti-inflammatory, anticancer and sedative etc. Therefore the present study was designed on synthesis and pharmacological evaluation of 2-(4-(3 (Substituted Phenyl) Acryloyl) Phenoxy)-N, N Diphenylacetamides. Target compound was synthesized by reaction of chloroacetylchloride with diphenylamine to afford 2-chloro-N, N-diphenylacetamide which further by reaction with substituted Chalcones and characterized following recrystallization and evaluated for anti-microbial potential through cup-diffusion method. In results, the target compounds were tested for activity against B. Subtilis, E.Coli and C. albicans. The chalcones having the lipophilic 4-chloro group (RKCT2) showed the greatest antimicrobial activity (zone of inhibition 20 & 22 mm against. B. subtilis, E. Coli, C. Albicans respectively. It suggests further researchers to go through anti-microbial evaluations against a more varieties of bacteria and fungi. Keywords: Schiff bases of diphenylamine derivatives, antibacterial activity, Gram-positive bacteria, 2-(4-(3 (Substituted Phenyl) Acryloyl) Phenoxy)-N, N Diphenylacetamides

scholarly journals IN VITRO ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF SEEDPOD AND QUERCETIN OF NELUMBO NUCIFERA GAERTN

2019 ◽  
Vol 12 (1) ◽  
pp. 164
Author(s):  
Ruvanthika Pn ◽  
Manikandan S
Keyword(s):  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Nelumbo Nucifera ◽  
Diffusion Method ◽  
Dependent Manner ◽  
E Coli ◽  
In Vitro Antibacterial Activity

Objective: The objective of the study was to evaluate whether ethanolic extracts of Nelumbo nucifera (EENN) seedpod and quercetin (active component of NN) possess antibacterial proprieties against Gram (-) bacteria such as Escherichia coli and Pseudomonas aeruginosa and Gram (+) bacteria such as Staphylococcus aureus. Methods: Antibacterial activities of EENN seedpod and quercetin were investigated using disc diffusion method, minimum inhibitory concentration against E. coli and P. aeruginosa and Gram (+) bacteria such as S. aureus. Results: The antibacterial activity of both EENN seedpod and quercetin was found to be increased in dose-dependent manner. The maximum zone of inhibition was exhibited by both EENN seedpod and quercetin against E. coli (14 mm and 15 mm) and P. aeruginosa (13 mm and 15 mm). Gram-negative bacteria were more susceptible to the EENN seedpod extract and quercetin than Gram-positive bacteria.Conclusion: The results of the present study suggested that the effect of EENN seedpod and quercetin against the tested bacteria in vitro may contribute to the in vivo activities of the EENN seedpod and quercetin.

scholarly journals In vitro antibacterial activity of several plant extracts against fish bacterial pathogens

2021 ◽  
Vol 13 (Aquaculture) ◽  
pp. 106-112
Author(s):  
Thi My Duyen Tran ◽  
Trong Tuan Nguyen ◽  
Thi Tuyet Hoa Tran
Keyword(s):  
Antibacterial Activity ◽  
Plant Extracts ◽  
Broad Spectrum ◽  
Alternative Therapy ◽  
Diffusion Method ◽  
Dilution Method ◽  
Fish Pathogens ◽  
Agar Disc ◽  
In Vitro Antibacterial Activity

Crude methanol extract of 9 Vietnamese plants were in vitro screened for their antibacterial activity against three common freshwater fish pathogens including Aeromonas hydrophila, Edwardsiella ictaluri, and Streptococcus agalactiae. Agar disc diffusion method was used to evaluate the antibacterial activity, then solvent extract was performed for the extracts which exhibited the strongest and a broad-spectrum antibacterial activity. Minimal inhibitory concentration (MIC) was conducted for effective plant extracts using broth dilution method. The results indicated that most of the plant extracts exhibited antibacterial propeties to at least one tested bacterium. Headache tree (Premna corymbosa), bushwillows (Combretum quadrangulare) and Celandine spider flower (Cleome chelidonii) showed a broad-spectrum antibacterial activity. The largest inhibitory zones of 35 mm and 21 mm were observed  for the extract of Premna corymbosa against E. ictaluri and S. agalactiae, respectively. E. ictaluri was found to be the most susceptible for all of the extracts while A. hydrophila was the most resistant. The MIC of effective plant extracts against tested bacteria ranged between 0.39 mg/mL and 3.125 mg/mL. The result can be considered for further investigation of the development of an alternative therapy against bacterial infection in aquaculture.

scholarly journals In Vitro Anti-Microbial Activity of Essential Oils and other Extracts from Salvia officinalis against Some Bacteria

2019 ◽  
Author(s):  
Amna Yousif Mohamed ◽  
Ahmed Ali Mustafa
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Salvia Officinalis ◽  
Diffusion Test ◽  
Hexane Extraction ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Agar Disc

This study aimed to screen the antibacterial activity of essential oils from different parts (leave and stem) of Salvia officinalis against some Gram positive and Gram negative bacteria using agar disc diffusion test, then the extracts were prepared by hydro distillation to extract the essential oils. Maceration and hexane extraction by Soxhlet were used to obtain crude extracts from the leave and stem. Essential oils from the leaves and the ethyl acetate extract of the leaves showed higher antimicrobial activity, while hexane extract of leaves and stems showed moderate antibacterial activity. In contrast the essential oil from the stems showed very low antibacterial activity. It was observed that the results gram positive bacteria (staphylococcus aureus) was more sensitive than Gram negative (Echerichia coli).

scholarly journals Antibacterial Activity and Chemical Composition of Red Peacock Flower (Caesalpinia pulcherrima L.) Leaf Essential Oil

2019 ◽  
Vol 22 (6) ◽  
pp. 269-274
Author(s):  
Novena Risnalani Rintank Constani ◽  
Hartati Soetjipto ◽  
Sri Hartini
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Leaf Essential Oil

Peacock flower (Caesalpinia pulcherrima L.) leaves contain essential oils which can be used as an ingredient in cosmetics, perfume, aromatherapy, medicine, and supplements. The study was conducted to obtain essential oils from peacock flower leaves and determine the antibacterial activity against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa). Antibacterial activity test was carried out by the agar diffusion method, using paper discs. Measurements were made for the inhibition zone diameter (IZD) that appeared, while the essential oil component was analyzed using GC-MS. The results showed that the peacock flower leaves (C. pulcherrima) had a moderate to strong antibacterial effect at a concentration of 7.5%-20% against gram-positive bacteria (B. subtilis and S. aureus) and gram-negative bacteria (E. coli and P. aeruginosa). Gram-negative E. coli bacteria are relatively more sensitive to peacock flower leaf essential oil compared to other test bacteria. Peacock flower (C. pulcherrima) leaf essential oil is composed of 7 main components namely β-Cubebene 33.87%; Caryophyllene 23.00%; γ-Elemene 13.18%; α-Pinene 10.96%; Cadina-1(10),4-diene 10.20%; Copaene; 7.09%; β-Pinene 1.70%.

scholarly journals Assessment of the antibacterial activity of four essential oils and the biobactericide Neco.

2017 ◽  
Vol 9 (3) ◽  
pp. 443 ◽  
Author(s):  
Jean-Fabrice Yala ◽  
Rolande Mabika Mabika ◽  
Brahima Camara ◽  
Seydou Tuo ◽  
Alain Souza ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Diffusion Method ◽  
Melaleuca Quinquenervia ◽  
Eucalyptus Citriodora ◽  
Cymbopogon Citratus ◽  
Gram Positive Bacteria ◽  
Lippia Multiflora ◽  
Microdilution Method

<p>The purpose of this study was to assess <em>in vitro</em> the activity of four essential oils (<em>Cymbopogon citratus, Eucalyptus citriodora, Lippia multiflora, Melaleuca quinquenervia</em>) and the biobactericide Neco® on Gram-positive bacteria.</p><p><strong>T</strong>he aromatogram and antibiogram were assessed by the agar well diffusion method and the Muller Hinton disk-agar diffusion method, respectively. Also, the minimum inhibitory concentration and the minimum bactericidal concentration were determined by the microdilution method in liquid medium.</p><p>The aromatogram showed that the biobactericide Neco® induced the largest inhibition diameters (34.53 ± 11.82 - 43.92 ± 5.38 mm) of all strains combined, followed by the essential oils of <em>Eucalyptus Citriodora</em> (30.01 ± 3.02 - 41.89 ± 1.77 mm) and <em>Lippia multiflora</em> (20.72 ± 4.72 - 37.61 ± 2.80 mm). However, the essential oils of <em>Melaleuca quinquenervia</em> (19.99 ± 3.93 - 26.20 ± 13.27 mm) and <em>Cymbopogon citratus</em> (13.52 ± 3.59 - 29.08 ± 2.35 mm) had the smallest inhibition diameters. Moreover, the comparison of the activities of the aromatogram and antibiogram revealed generally that activities were higher with essential oils than with antibiotics.</p><p>At the end of this study, the essential oils of <em>Cymbopogon citratus</em>, <em>Eucalyptus citriodora, Lippia multiflora, Melaleuca quinquenervia</em> and the biobactericide Neco® had an antibacterial activity on Gram+ bacteria.</p><p><strong><br /></strong></p>

scholarly journals In vitro Antibacterial Activity Analysis of Leaves of Limonia acidissima

2014 ◽  
Vol 6 (2) ◽  
pp. 155-157
Author(s):  
Gandi Kishore NAIDU ◽  
B. SUJATHA ◽  
K. Chandra Sekhar NAIDU
Keyword(s):  
Antibacterial Activity ◽  
Micrococcus Luteus ◽  
Chloroform Extract ◽  
Diffusion Method ◽  
Moderate Activity ◽  
Phytochemical Screening ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Limonia Acidissima

The present study was carried out the antibacterial activity and phytochemical screening of the hexane, chloroform and methanol extracts of leaves of Limonia acidissima. The antibacterial activity was evaluated against four Gram-negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus vulgaris) and five Gram-positive bacteria (Bacillus subtilis, Enterococcus faecalis, Micrococcus luteus, Staphylococcus aureus, Streptococcus pneumoniae) by agar well diffusion method. Methanol extract showed good antibacterial activity with the high inhibition zones while chloroform extract exhibited mild to moderate activity and hexane extract was found to be less active. Phytochemical screening revealed the presence of various secondary metabolites like steroids, alkaloids, phenols, flavonoids, coumarins, saponins, tannins and triterpenoids. The results of the present study suggest that leaves of Limonia acidissima can be used to treating infectious diseases caused by E. coli, P. vulgarisand S. pneumoniae.

scholarly journals In Vitro Antibacterial Activity of Black Soldier Fly (Hermetia Illucens) Larva Extracts Against Gram-Negative Bacteria

2019 ◽  
Vol 43 (2) ◽  
Author(s):  
Harlystiarini Harlystiarini ◽  
Rita Mutia ◽  
I Wayan Teguh Wibawan ◽  
Dewi Apri Astuti
Keyword(s):  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Test Method ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Black Soldier Fly ◽  
E Coli ◽  
In Vitro Antibacterial Activity ◽  
Zone Growth

The aim of this study was to evaluate the in vitro antibacterial activity of Black soldier fly (BSF) larva extract. The BSF larva was extracted using methanol and then tested for antibacterial activity using agar diffusion method (zone growth inhibition). The antibacterial activity was conducted against Salmonella sp. and Escherichia coli, two important bacterial strains in poultry, using six dilution levels (10 mg/ml, 20 mg/ml, 40 mg/ml, 80 mg/ml, 160 mg/ml and 320 mg/ml). All the results were subjected analyze using t-test method. Based on the diameter of the inhibition zone, the BSF larva extract has a strong (P<0.05) antibacterial activity against Salmonella sp. and E. coli when the concentration used 320 mg/ml. In addition, BSF larva extract also contain high amount of lauric acid (49.18%), a saturated fatty acid that has been proven to proposes as antibacterial agent. Therefore, it could be concluded that the BSF larva extract could be used as a candidate for antibacterial substances. 

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