Chronic nandrolone decanoate administration effects on antioxidant parameters in Wistar rats

2021 ◽  
Vol 26 (2) ◽  
pp. 2483-2488
Author(s):  
A.E. ROŞCA ◽  
◽  
L. IOSIF ◽  
I.C. TIVIG ◽  
R. MIRICA ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Wistar Rats ◽  
Treated Group ◽  
Total Antioxidant Activity ◽  
Nandrolone Decanoate ◽  
Advanced Oxidation Protein Products ◽  
Paraoxonase Activity ◽  
Total Antioxidant ◽  
High Doses

Chronic nandrolone decanoate exposure is associated with a modified redox status in the favor of oxidants. Paraoxonase and myeloperoxidase activities are considered sensitive biomarkers of oxidative stress and inflammation, being also predictors for coronary artery disease. The aim of our study was to investigate the impact of chronic nandrolone administration on paraoxonase and myeloperoxidase activities in Wistar rats. 16 Wistar rats were divided into two groups (n=8 for each of them): nandrolone decanoate (A) treated group and control group (C). After 12 weeks of nandrolone decanoate administration we analyzed several plasma oxidative stress parameters: TROLOX Equivalent Antioxidant Activity, paraoxonase and myeloperoxidase activities, total thiols, and advanced oxidation protein products. Our results showed a significant decreased total antioxidant activity in nandrolone treated group compared with controls, while paraoxonase activity and total thiols levels were significantly increased. Myeloperoxidase activity and advanced oxidation protein products were not significantly different between the two studied groups. In conclusion decreased plasma total antioxidant activity suggested increased oxidative stress induced by chronic high doses of nandrolone. Despite oxidative stress presence high doses of nandrolone induced also increased plasma paraoxonase activity.

scholarly journals Oxidative Products of Proteins and Antioxidant Potential of Thiols in Gastric Carcinoma Patients

2010 ◽  
Vol 29 (2) ◽  
pp. 102-106 ◽  
Author(s):  
Anita Mukthapura ◽  
Avinash Shimogga ◽  
Vinodchandran K ◽  
Beena Shetty ◽  
Gayathri Rao
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Gastric Carcinoma ◽  
Advanced Oxidation ◽  
Antioxidant Potential ◽  
Total Antioxidant Activity ◽  
Advanced Oxidation Protein Products ◽  
Total Antioxidant ◽  
Oxidative Products ◽  
Primary Gastric Carcinoma

Oxidative Products of Proteins and Antioxidant Potential of Thiols in Gastric Carcinoma PatientsIt has been suggested that oxidative stress defined as a shift in antioxidant/oxidant balance towards oxidants is associated with the pathogenesis of many diseases, including carcinogenesis. Reactive oxygen species can induce carcinogenesis via injury to macromolecules such as DNA, carbohydrates and proteins. Forty primary gastric carcinoma patients and 40 healthy controls were included in the study. Advanced oxidation protein products, total thiols, total protein, albumin in plasma, % hemolysis in RBC suspension and glutathione in both whole blood and plasma were estimated. Our studies demonstrated a significant increase in advanced oxidation protein products, % hemolysis (p=0.033), A:G ratio (p=0.003) and a highly significant decrease in blood glutathione (p=0.036), total thiols (p=0.001), plasma thiols other than glutathione and total antioxidant activity. The findings suggest that gastric carcinoma is associated with oxygen derived free radicals accumulation, and depletion of total antioxidant activity has lead to oxidative stress and advancement of oxidative-antioxidative disorders followed by progression of gastric cancer.

The state of the blood antioxidant system in the patients presenting with acromegaly

2015 ◽  
Vol 61 (2) ◽  
pp. 8-11
Author(s):  
M V Faassen ◽  
M S Pankratova ◽  
N N Molitvoslovova ◽  
A A Baizhumanov ◽  
S S Kovalenko ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Blood Plasma ◽  
Antioxidant System ◽  
The State ◽  
Total Antioxidant Activity ◽  
Sod Activity ◽  
Protein Thiols ◽  
Total Antioxidant

The state of the blood antioxidant system in the patients presenting with acromegaly. The objective of the present study was to evaluate the total antioxidant activity (TAA) of blood plasma, the levels of non-protein thiols and 2-thyobarbituric acid-active products (TBA-AP), superoxide dismutase (SOD) and catalase (CAT) activities as well as ceruloplasmin (CP) level in the patients presenting with acromegaly. It was shown that plasma TAA and SOD activity in this patients was on the average 20 and 30% lower respectively than in the control subjects. At the same time, the TBA-AP and CP levels increased by 50 and 40% respectively. These data suggest the development of oxidative stress in the acromegalic patients.

scholarly journals Antioxidant Potential and Peroxidative State of `Golden Smoothee' Apples Treated with 1-Methylcyclopropene

2006 ◽  
Vol 131 (1) ◽  
pp. 104-109 ◽  
Author(s):  
Rosa Vilaplana ◽  
M. Carme Valentines ◽  
Peter Toivonen ◽  
Christian Larrigaudière
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Storage Period ◽  
Antioxidant Potential ◽  
Total Antioxidant Activity ◽  
Pulp Tissue ◽  
Antioxidant Metabolism ◽  
Beneficial Effects ◽  
Total Antioxidant

In order to determine the effects that 1-methylcyclopropene (1-MCP) may have on antioxidant metabolism during cold storage, apples (Malus ×domestica Borkh. cv. Golden Smoothee) were treated with 625 nL·L−1 1-MCP immediately after harvest and stored in air for 3 months. Differences in total antioxidant activity and ascorbate levels were determined during storage and related to the activity of the antioxidant enzymes superoxide dismutase [SOD (EC 1.15.1.1)], catalase [CAT (EC 1.11.1.6)], and peroxidase [POX (EC 1.11.1.7)] in pulp. The level of oxidative stress in the pulp tissue was also established by determining changes in levels of hydrogen peroxide and in the content of peroxidative markers during storage. Controls and 1-MCP-treated fruit exhibited similar changes in total antioxidant activity and ascorbate levels. However, significant differences in oxidative stress levels were found between treated and untreated fruit. 1-MCP-treated fruit exhibited lower levels of hydrogen peroxide and significantly lower levels in peroxidative markers, especially at the end of the storage period. In line with this last result, 1-MCP-treated fruit also exhibited greater enzymatic antioxidant potential and, more specifically, a higher level of POX activity. Collectively, these results showed that 1-MCP did not detrimentally affect the antioxidant potential of the fruit and provided evidence to support the hypothesis that the beneficial effects of 1-MCP on ripening are not exclusively limited to its effect on ethylene, but also include direct effects on peroxidation and POX enzyme activity.

scholarly journals Parameters of Lipid Peroxidation and Antioxidant Protection in Men with Pathological Spermogram Variants

2019 ◽  
Vol 4 (1) ◽  
pp. 14-18
Author(s):  
Nadezhda A. Kurashova ◽  
Ekaterina A. Kudeyarova ◽  
Ekaterina O. Kuznetsova
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Total Antioxidant Activity ◽  
Antioxidant Protection ◽  
Defense System ◽  
Total Antioxidant ◽  
Peroxidation Processes

Background.Today infertile marriage is not only a serious medical, but also a socio-demographic and economic problem. Male factor contributes averagely to half of the cases of the disease in couples. Such factors as high levels of reactive oxygen species and oxidative stress have been reported to compromise the process of spermatogenesis and sperm function in men. Oxidative stress is a significant risk factor for male infertility. A pro-oxidant testicular environment may alter the expression profile of functional sperm proteins and result in poor sperm quality.Aims.To study the characteristics of the intensity of the processes of lipoperoxidation and antioxidant status in the ejaculate of men with different variants of spermograms.Materials and methods.We examined 69 men with primary infertility and 155 fertile men. The content of lipid peroxidation components and antioxidant protection was determined by spectrophotometric method.Results.The results of the study in men with infertility and asthenozoospermia showed decreased total antioxidant activity of sperm by 50 % and α-tocopherol by 52 %, and in men with infertility and oligozoospermia, decreased total antioxidant activity of sperm by 47 % and α-tocopherol by 41 %.Conclusions.The analysis indicates a change in the parameters of the system of lipid peroxidation – antioxidant defense system and confirms the development of oxidative stress in them. Depending on the pathological state of the ejaculate in men of reproductive age, lipid peroxidation processes have their own characteristics. In men with oligozoospermia, peroxidation processes occur more intensively. Activation of lipid peroxidation – antioxidant defense system processes can be both a consequence and a cause of various metabolic changes in the human body.

scholarly journals Effect of dietary supplementation with rocket salad (Eruca sativa) seeds powder on certain seminal plasma traits of Hy – line laying breeder roosters subjected to oxidative stress induced by hydrogen peroxide

2012 ◽  
Vol 36 (0A) ◽  
pp. 62-69
Author(s):  
Hazim J. Al – Daraji
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Antioxidant Activity ◽  
Seminal Plasma ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Control Group ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

This study was conducted to evaluate the effect of adding different levels of rocket salad seeds powder to the diet on seminal plasma traits of roosters subjected to oxidative stress induced by hydrogen peroxide. A total of 60 Hy – line laying breeder roosters 57 weeks old were used in this study. Roosters were randomly distributed into 5 treatments with 3 replicates each. Each replicate constituted of 4 roosters (12 roosters for each treatment). Experimental treatments were as following: T1: Males fed control diet and normal water, T2: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 0.25 ml hydrogen peroxide (0.5%) / litter of water, T3: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 0.5 ml hydrogen peroxide (0.5%) / litter of water, T4: Males fed diet supplemented with 3 gm rocket salad powder / kg of diet + 1 ml hydrogen peroxide (0.5%) / litter of water, and T5: Males fed control diet and drink tap water supplemented with 1 ml hydrogen peroxide (0.5%) / litter of water. Males were treated with hydrogen peroxide (6%) and rocket salad for 12 weeks starting from 59 week of male ages. Results revealed that treated the roosters with hydrogen peroxide without adding rocket salad powder to the diet of these roosters (T5) resulted in highly significant (p< 0.01) decrease as regards concentrations of phospholipids, cholesterol, glutathione, the activity of superoxide desmutase and catalase, and total antioxidant activity in seminal plasma and highly significant (p< 0.01) increase concerning concentrations of tyrosine and malondialdehyde as compared with control group (T1) and rocket salad powder treatments (T2, T3, T4) after 12 weeks of experiment. However, supplementing diet of roosters with rocket salad powder (T2, T3, T4) resulted in highly significant (p< 0.01) increase with relation to concentrations of phospholipids, cholesterol, glutathione, the activity of superoxide desmutase and catalase, and total antioxidant activity in seminal plasma and highly significant (p< 0.01) decrease respecting concentrations of tyrosine and malondialdehyde as compared with (T5) In conclusion adding rocket salad powder to the diet of roosters had important role in limiting the negative effect of oxidative stress induced by hydrogen peroxide on seminal plasma quality of roosters. Therefore, dietary supplementation with rocket salad powder could be used as one of important tools for improving semen quality of roosters.

Effects of Aerobic Exercise on Uric Acid, Total Antioxidant Activity, Oxidative Stress, and Nitric Oxide in Human Saliva

2008 ◽  
Vol 16 (2) ◽  
pp. 128-137 ◽  
Author(s):  
David González ◽  
Ramón Marquina ◽  
Norelis Rondón ◽  
Antonio J. Rodríguez-Malaver ◽  
Rafael Reyes
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Uric Acid ◽  
Aerobic Exercise ◽  
Human Saliva ◽  
Total Antioxidant Activity ◽  
Total Antioxidant

scholarly journals Assessment of oxidative stress indicators of West African Dwarf sheep under semi intensive management system

2021 ◽  
Vol 43 (2) ◽  
pp. 78-83
Author(s):  
A. A Jack ◽  
O. A. Jimoh ◽  
U. G. Ihejirika ◽  
U. C. Uwaeziozi ◽  
O. O. Adenekan
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Serum Biochemistry ◽  
West African ◽  
Total Antioxidant Activity ◽  
West African Dwarf ◽  
Total Antioxidant ◽  
Serum Biochemical

Oxidative stress results from increased production of free radicals and reactive oxygen species, and a decrease in antioxidant defense. This study was conducted to assess sexual dimorphism in serum biochemistry and oxidative status of West African dwarf lambs. Sixteen each of rams and ewes 8months old were randomly selected from a herd purchase from livestock market, Oyo. The average body weights of the lambs were 12.08±3.26 and 12.76kg ewes and rams, respectively. Fastened blood samples were collected from the sheep and assayed for serum biochemical, lipid peroxidation, total antioxidant activity, catalase and superoxide dismutase using standard procedures, data obtained were subjected to T-test. The result indicated that both sex had similar serum biochemical indices except total protein. Ewes had significantly higher serum protein than ram. Rams had apparently higher total antioxidant activity and significantly higher serum catalase activity than ewes, while serum lipid peroxidation and superoxide dismutase were statistically similar in both sexes. This indicates that rams have better antioxidant activity than ewes. During oxidative stress prone conditions and environment, measures to boost antioxidant activity of ewes should be emphasized.

scholarly journals Evaluation of oxidative stress and antioxidant activity in pre and post hemodialysis in chronic renal failure patients from Western region of Nepal

2013 ◽  
Vol 13 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Takkella Nagamma ◽  
S Ahmed ◽  
Akshatha Pai ◽  
Sheethal Mohan ◽  
Abhishek Chathuvedi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Renal Failure ◽  
Chronic Renal Failure ◽  
Vitamin C ◽  
Oxidative Stress Marker ◽  
Cellulose Membrane ◽  
Total Antioxidant Activity ◽  
Plasma Urea ◽  
Total Antioxidant

Objective: To evaluate the oxidative stress and antioxidant activity in pre and post hemodialysis in chronic renal failure patients using a diacetate cellulose membrane. Background: Chronic Renal Failure is a gradual, progressive and irreversible loss of normal functioning of kidneys. This is associated with increased formation of reactive oxygen species and increased oxidative stress. No studies have been undertaken in this area in Nepal. Method: The study groups were devided into chronic renal failure patients undergoing hemodialysis (n=52) and healthy controls (n=52). Diacetate cellulose was used as dialyzer membrane for chronic renal failure patients. Blood samples were collected in EDTAbottles and plasma was used for the estimation of oxidative stress marker, total antioxidant activity, vitamin C, ?-tocopherol, urea and creatinine. Results: There was significant reduction in the levels of vitamin C,? -tocopherol and total antioxidant activity in post dialysis patients compared to predialysis and control groups (p<0.001). Plasma urea and creatinine levels were high in predialysis patients compared to controls and was significantly reduced after dialysis. Significantly elevated malondialdehyde levels were found in chronic renal failure patients before and after dialysis when compared with controls. Conclusion: The results of our study indicate that there are reduced levels antioxidants in chronic renal patients in pre and post dialysis condition and also more loss of antioxidants after dialysis. All these condition leads to increased oxidative stress in these patients. DOI: http://dx.doi.org/10.3329/bjms.v13i1.14741 Bangladesh Journal of Medical Science Vol. 13 No. 01 January2014: 40-44

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