scholarly journals Efficiency of selection of self-fertile sunflower biotypes in production of foundation and breeder seeds

Author(s):  
A.D. Bochkovoy ◽  
◽  
V.I. Khatnyansky ◽  
V.A. Kamardin ◽  
D.A. Nazarov ◽  
...  

The researches were conducted on fields of OOO NPO “Triumph”, the Matveevo-Kurgansky district of Rostov region and V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar in 2017–2020. The purpose of the research was to develop methodology of selection of self-fertile sunflower genotypes in foundation and breeder seed production. Our work resulted in development of improved population Dobrynya plus. Middle self-fertility of this population at self-pollination was 13.7% vs. 5.2% of the initial variety (increased by 2.6 times), a ratio of the most valuable genotypes with amount of fully formed seeds more than 150 seeds per a plant increased from 13.5 to 26.4% (by 1.9 times). At the open flowering and free insect pollination, population Dobrynya plus does not differ by its economically valuable traits from the initial variety. But at the close flowering, without insects pollination, yield of the population Dobrynya plus was 0.93 t per ha vs. 0.52 t per ha of the initial variety (increase by 1.8 times). At selection of elite seeds from sunflower varietal populations for seed growing, the most perspective are the biotypes with high level of self-fertility which traits are held stable in the different environments. Improvement of sunflower variety by 11 self-fertility does not lead to decreasing of its yield qualities by the main economically valuable traits. The obtained experimental data prove a prospectivity of researches directed on improvement of sunflower variety populations by self-fertility at production of foundation and breeder seeds and possibility to reach successful results.

Author(s):  
A.D. Bochkovoy ◽  
◽  
V.A. Kamardin ◽  
D.A. Nazarov ◽  
◽  
...  

In 2017–2019 we conducted researches in NPO “Triumph”, Matveevo-Kurgansky district, Rostov region, and in fields of V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar. The purpose of the research was to develop methodic questions of selection of self-fertile biotypes during production of foundation seeds of confectionary sunflower varieties. We stated self-sterile and semisterile consist the main part of the studied seven OPvarieties and are equal 68.5% in average. They vary from 60.3% in the Enisey variety to 75.0% in Dobrynya. Basing on our researches we proposed a rating scale for self-fertility level in sunflower varieties and a scheme of foundation seeds growing oriented for selection of biotypes with increased selffertility. We developed a promising varietypopulation Dobrynya+, its average self-fertility of plants is equal to 12.2% comparing to 6.5% of the initial variety. A share of the less valuable self-sterile biotypes in it decreased from 22.2 to 12.0%, and a portion of the most valuable highly self-fertile, contrariwise, increased from 13.9 to 18.0%. By a complex of the other economically valuable traits a population Dobrynya+ does not differ of the initial variety.


Author(s):  
S.V. Zelentsov ◽  
◽  
E.V. Moshnenko ◽  
T.P. Shuvaeva ◽  
I.V. Gaytotina ◽  
...  

The main receptacle of essential oil in true lavender is the peltate glandular trichomes of the calyxes in the whorls of the inflorescences. Their average size is 175 ± 25 µm, in some cases – up to 250 µm. For the extraction of lavender oil, not only the calyxes are used, but the whole inflorescences including the flowering shoots. The surface of the peduncles of lavender inflorescences is also covered with peltate glandular trichomes. However, their contribution to the total volume of essential oil in the inflorescence has almost never been determined. The aim of this research was to study the distribution density of glandular trichomes within the inflorescence and to determine the proportion of the contribution of flowering trichomes to the formation of essential oil in the inflorescence. The research was carried out in 2021 on the basis of the V.S. Pustovoit All-Russian Research Institute of Oil Crops in two ecological and geographical points of the Krasnodar region. The object of the study was the true lavender varieties Voznesenskaya 34, Rannyaya, Yuzhanka and Voznesenskaya Aroma. It was found that the size of glandular trichomes on peduncles of true lavender is 90 ± 15 µm. Their number on peduncles, depending on the variety, varies from 2141 to 3003 pcs. The density of distribution of glandular trichomes on the surface of peduncles is equal to 8.60–14.93 pcs/mm3 . The total volume of essential oil in all glandular trichomes of peduncles is 0.41–0.57 cmm . The total volume of essential oil in the inflorescences varied from 2.28 to 5.15 cmm . The share of essential oil in the glandular trichomes of the peduncles in relation to the entire inflorescence ranged from 9.33 to 19.56%. It is concluded that peltate glandular trichomes on flower-bearing axes make a significant contribution to the essential oil content of lavender inflorescences. For the selection of true lavender to increase the essential oil content and the yield of essential oil, an additional selection trait is proposed – the amount of glandular trichomes on the surface of peduncles, which can be regulated by changing their distribution density on the surface of peduncles, or increasing the length of inflorescences.


Author(s):  
D.L. Savichenko ◽  
◽  
S.Z. Guchetl ◽  
A.V. Golovatskaya ◽  
◽  
...  

Studying of sunflower lines resistant to broomrape race G, bred in V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK), Krasnodar showed the genetic control of resistance is monogenic with incomplete dominance. The purpose of the research was to seek and analyze molecular markers for the genes of resistance to broomrape race G using PCRmethods. In our research we used six developed in VNIIMK lines-donors resistant to broomrape race G: RGP1, RGP2, RGB, RGL1, RGL2, RGA, and susceptible lines VK 101, VK 678, VK 680. We extracted DNA from the leaves of young sunflower plants using STAB-buffer. For PCR-analysis we used 17 pairs of primers of two types: SCAR (sequence characterized amplified region) and SSR (simple sequence repeat). Conditions of amplification are as recommended by authors of markers with experimental selection of a temperature for primers hybridization. Within the research we balanced involved into work SSR- and 12 SCAR-loci using BLAST and a set of HanXRQr1.0. Due to these data we composed a physical map of loci location. After assessment of six resistant and three susceptible parental lines, seven markers demonstrated polymorphism by a length of locus DNA and two ones – by presence/absence of amplified fragment. For markers ORS 683 and ORS 1112 we observed a relation between loci polymorphism and susceptibility of parental lines to broomrape. This was certified with a presence of an allele of 364 n.p. length in ORS 683 and an absence of an allele of 375 n.p. length in ORS 1112. These markers were noted as primary ones for hybridological analysis in F1 и F2 with selected pairs for crossing. Additional markers will be selected individually using obtained data on loci polymorphism. Thus, as a result of the research we created a physical map for the further markers selection, found hybrid combinations and markers from the studied ones for hybridological analysis.


2019 ◽  
pp. 44-47 ◽  
Author(s):  
G. Ya. Krivosheev ◽  
A. S. Ignatiev ◽  
A. G. Gorbacheva ◽  
I. A. Vetoshkina

The purpose of the study is the application of the method of multi-criteria estimation to select the initial material of various direction of maize breeding. The studies were performed in the year of 2017–2018 in two different regions of trials, in the arid area of the ARC “Donskoy” (Zernograd) and in the moisture area of the All-Russian Research Institute of Maize (Pyatigorsk). 10 parental forms of maize hybrids ‘Alpha M’, ‘Almaz M’, ‘Avrora S’, ‘Milena M’, ‘Istok S’, ‘Mirt M’, ‘Malvina S’, Raduga S’, Maya M’, ‘Prestizh M’ used as testers in breeding programs were taken as initial material. The studied forms were characterized by a diversity in the value of their main economically valuable traits. Significant variability was identified in infertility of plants (V = 56.0%), smut infection (V = 46.4%) and fragility of a stem below a cob (V = 43.4%). Average variability was identified in grain productivity (V = 14.8–18.2%), drought resistance index (V = 10.5%), harvesting moisture of grain (V = 18.6%). The use of the complex of main economically valuable traits for the integrated assessment (SD), taking into account the specified contributions (Rk) and weight coefficients (Wi), made it possible to rank the initial material according to its value for various areas of selection. There have been identified the testers ‘Mirt M’, Maya M, ‘Istok S’ with the best integral values (SD = 2.63–5.65) for breeding on drought tolerance. ‘Istok S’, ‘Milena M’, ‘Avrora S’ (SD = 2.14–4.07) have been recommended as the testers in breeding programs to breed the varieties with low harvesting moisture of grain. There has been identified a universal tester ‘Istok S’ which is suitable for various directions of maize breeding. Thus, the multi-criteria estimation can be used in the breeding process to select initial material that meets the requirements of the planned breeding directions.


Author(s):  
S.V. Sklyarov ◽  
◽  
L.G. Ryabenko ◽  
V.S. Zelentsov ◽  
L.R. Ovcharova ◽  
...  

The researches were conducted in the laboratory of oil flax breeding of the V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar 2019– 2020. We studied the samples from the highly oleic collection of flax of the V.S. Pustovoit All-Russian Research Institute of Oil Crops in order to select the best ones for introduction into a breeding process to improve the existing varieties and develop new ones with a good combination of biological and economically valuable traits. The samples from our collection demonstrated variability by the general economically valuable and biological traits. Thus, difference in duration of a period emergence–maturing was from 10 to 12 days depending on year and weather conditions. Maximal plant height (63.3 сm) was noted in 2020, and minimal (38.0 cm) was similar in all years of the research. Seed yield per a plot varied from 32.5 to 112.5 g/m 2 in 2019 and from 30.0 to 175.0 g/m 2 in 2020. The highest yield (175.0 g/m2) was fixed in 2020, and minimal one (30.0 g/m2) was at the same level in all years of the research. The samples from the collection were differed by their oil content in seeds: from 44.6 to 48.7% in 2019 and from 44.8 to 49.3% in 2020. Variation of this trait by years was 4.1–4.5%, difference between years of the research was equal to 0.4%. The presence of variability in oil contents in seeds among samples from flax collection proves a possibility to conduct selections by this trait in all breeding nurseries of the laboratory. Thus, in 2020 we selected two samples with oil content in seeds more than 49.0%.


Russian vine ◽  
2021 ◽  
Vol 15 ◽  
pp. 11-18
Author(s):  
S.I. Krasokhina ◽  

The most promising way to reduce the need for fungicides in viticulture is the use of re-sistant cultivars. In this regard, an urgent task is the selection of table grape varieties with increased resistance to diseases and frost in combination with high marketability and taste. The article discusses the results of a fif-teen-year study of the promising table variety Aladdin. The variety is interspecific hybrid obtained from a saturating crossing Vostorg krasnyy × Vostorg muskatniy at All-Russian Research Institute for Viticulture and Wine-making– Branch of Federal State Budget Sci-entific Institution «FRARC». The Aladdin variety was compared with the regionalized table variety Vostorg, which is also the paren-tal form in the second generation. The viticul-ture is not irrigated, grafted, not covered, the training is fan type on trunk, the planting scheme is 3 × 1.5 m. In the variety study, the methods generally accepted in viticulture and the standard technology of cultivation of vineyards were used. The Aladdin variety is distinguished by an early ripening period, large berries, medium-sized bunches, firm pulp, attractive berry color, light muscat aro-ma. The variety has a proven genetic high re-sistance to powdery mildew and mildew, high frost resistance is confirmed by the data of agrobiological records. According to the total-ity of economically valuable traits, resistance to unfavorable biotic and abiotic environmen-tal factors, the Aladdin variety can be recom-mended for transfer to the State variety test-ing of the Russian Federation.


Author(s):  
Ya.N. Demurin ◽  
◽  
V.D. Savchenko ◽  
O.M. Borisenko ◽  
A.N. Levutskaya ◽  
...  

A simple sunflower hybrid Taizar was developed at V.S. Pustovoit All-Russian Research Institute of Oil Crops as part of a breeding and genetic program for developing highly productive hybrids resistant to the new virulent races of broomrape by using the method of backcrossing in combination with an evaluation of resistance in each generation for the target gene Or7. The hybrid Taizar is of mid-early group of ripeness, it has a high yield, it is resistant to Orobanche cumana Wallr. (races A-G) and downy mildew (races 330, 710, 730, 334, 734). The line-restorer of pollen fertility VK 305 is the paternal form of the hybrid Taizar. The line was developed as a result of the introduction of the Or7 gene from the RG line by two backcrosses into the line VK 303, subsequent inbreeding and selection of broomrape-resistant genotypes. The line VK 305 has narrow widely spaced ray flowers strongly curved towards the back of the head, which can serve as a morphological marker of genetic purity.


2021 ◽  
Author(s):  
D.V. Starikova ◽  

One of the methods to improve initial germplasm by genetic way for development of lines which are stable by their economically valuable traits us inbreeding. Inbreeding allows disclosing a huge potential of inheritance of traits in variety. Impact of inbreeding on formation of yield, structural elements, oil quality and content has been noted by a range of scientists for many years. Using the inbreeding, researchers of the V.S. Pustovoit All-Russian Research Institute of Oil Crops developed rapeseed cultivars characterized with high yield, oil content and other valuable biochemical traits, requiring to the demands of agrarian and possessing industries.


Sign in / Sign up

Export Citation Format

Share Document