scholarly journals The number of glandular trichomes on the peduncles of true lavender inflorescences as an additional breeding trait for essential oil

2021 ◽  
Vol 188 (4) ◽  
pp. 41-52
Author(s):  
S.V. Zelentsov ◽  
◽  
E.V. Moshnenko ◽  
T.P. Shuvaeva ◽  
I.V. Gaytotina ◽  
...  
Keyword(s):  
Essential Oil ◽  
Oil Content ◽  
Significant Contribution ◽  
Distribution Density ◽  
Glandular Trichomes ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Average Size ◽  
Selection Of ◽  
Russian Research

The main receptacle of essential oil in true lavender is the peltate glandular trichomes of the calyxes in the whorls of the inflorescences. Their average size is 175 ± 25 µm, in some cases – up to 250 µm. For the extraction of lavender oil, not only the calyxes are used, but the whole inflorescences including the flowering shoots. The surface of the peduncles of lavender inflorescences is also covered with peltate glandular trichomes. However, their contribution to the total volume of essential oil in the inflorescence has almost never been determined. The aim of this research was to study the distribution density of glandular trichomes within the inflorescence and to determine the proportion of the contribution of flowering trichomes to the formation of essential oil in the inflorescence. The research was carried out in 2021 on the basis of the V.S. Pustovoit All-Russian Research Institute of Oil Crops in two ecological and geographical points of the Krasnodar region. The object of the study was the true lavender varieties Voznesenskaya 34, Rannyaya, Yuzhanka and Voznesenskaya Aroma. It was found that the size of glandular trichomes on peduncles of true lavender is 90 ± 15 µm. Their number on peduncles, depending on the variety, varies from 2141 to 3003 pcs. The density of distribution of glandular trichomes on the surface of peduncles is equal to 8.60–14.93 pcs/mm3 . The total volume of essential oil in all glandular trichomes of peduncles is 0.41–0.57 cmm . The total volume of essential oil in the inflorescences varied from 2.28 to 5.15 cmm . The share of essential oil in the glandular trichomes of the peduncles in relation to the entire inflorescence ranged from 9.33 to 19.56%. It is concluded that peltate glandular trichomes on flower-bearing axes make a significant contribution to the essential oil content of lavender inflorescences. For the selection of true lavender to increase the essential oil content and the yield of essential oil, an additional selection trait is proposed – the amount of glandular trichomes on the surface of peduncles, which can be regulated by changing their distribution density on the surface of peduncles, or increasing the length of inflorescences.

scholarly journals The effect of herbicides on winter false flax (Camelina sativa (L.) Crantz.)

2021 ◽  
Vol 32 ◽  
pp. 02011
Author(s):  
Oksana Serdyuk ◽  
Victoria Trubina ◽  
Lyudmila Gorlova
Keyword(s):  
Oil Content ◽  
Plant Density ◽  
Central Zone ◽  
Application Rate ◽  
Oil Crops ◽  
False Flax ◽  
Russian Research Institute ◽  
Different Types ◽  
Flax Seeds ◽  
Russian Research

The purpose of the research was to determine the effect of herbicides on winter false flax and to identify the preparation that does not effect negatively on crop plants. The research was carried out in 20182020 at V.S. Pustovoit All-Russian Research Institute of Oil Crops. The experiment scheme included herbicides with active ingredients (a.i.), which effectively decreased the number of weeds on the plots. The effectiveness of the preparations was more than 70% for different types of weeds. However, the variants with the application of preparations with a.i. clopyralid 300 g/l, S-Metolachlor 960 g/l, ethametsulfuronmethyl 750 g/kg significantly decreased the plant density (by 18-32 pcs/m2) and seed yield (by 0.33-0.52 t/ha) of false flax in comparison with the control. The oil content of seeds was significantly decreased in the variants with the herbicides with a.i. S-Metolachlor 960 g/l with the application rate of 1.6 l/ha and ethametsulfuron-methyl 750 g/kg (by 1.2-1.5 %). In other variants, the oil content of false flax seeds differed from the control insignificantly (by 0.3-0.4 %). It has been established that the preparation with a.i. quinmerac 83 g/l + metazachlor 333 g/l with the application rate of 2.0 or 2.5 l/ha should be applied to decrease the number of weeds in the sowings of winter false flax in the central zone of the Krasnodar region. This preparation, without having a toxic effect, increases the yield by 0.15-0.17 t/ha and does not decrease the plant density and oil content of false flax seeds.

scholarly journals Efficiency of selection of self-fertile sunflower biotypes in production of foundation and breeder seeds

2021 ◽  
Vol 185 (1) ◽  
pp. 10-17
Author(s):  
A.D. Bochkovoy ◽  
◽  
V.I. Khatnyansky ◽  
V.A. Kamardin ◽  
D.A. Nazarov ◽  
...  
Keyword(s):  
Oil Crops ◽  
Rostov Region ◽  
Russian Research Institute ◽  
Economically Valuable Traits ◽  
Breeder Seed ◽  
High Level ◽  
Efficiency Of Selection ◽  
Seed Growing ◽  
Selection Of ◽  
Russian Research

The researches were conducted on fields of OOO NPO “Triumph”, the Matveevo-Kurgansky district of Rostov region and V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar in 2017–2020. The purpose of the research was to develop methodology of selection of self-fertile sunflower genotypes in foundation and breeder seed production. Our work resulted in development of improved population Dobrynya plus. Middle self-fertility of this population at self-pollination was 13.7% vs. 5.2% of the initial variety (increased by 2.6 times), a ratio of the most valuable genotypes with amount of fully formed seeds more than 150 seeds per a plant increased from 13.5 to 26.4% (by 1.9 times). At the open flowering and free insect pollination, population Dobrynya plus does not differ by its economically valuable traits from the initial variety. But at the close flowering, without insects pollination, yield of the population Dobrynya plus was 0.93 t per ha vs. 0.52 t per ha of the initial variety (increase by 1.8 times). At selection of elite seeds from sunflower varietal populations for seed growing, the most perspective are the biotypes with high level of self-fertility which traits are held stable in the different environments. Improvement of sunflower variety by 11 self-fertility does not lead to decreasing of its yield qualities by the main economically valuable traits. The obtained experimental data prove a prospectivity of researches directed on improvement of sunflower variety populations by self-fertility at production of foundation and breeder seeds and possibility to reach successful results.

scholarly journals Search and analysis of nuclear sequences in sunflower genome associated with genes of resistance to bromrape (Orobanche cumana Wallr.) races

2020 ◽  
Vol 183 (3) ◽  
pp. 11-19
Author(s):  
D.L. Savichenko ◽  
◽  
S.Z. Guchetl ◽  
A.V. Golovatskaya ◽  
◽  
...  
Keyword(s):  
Physical Map ◽  
Pcr Analysis ◽  
Sequence Characterized Amplified Region ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Parental Lines ◽  
Sunflower Genome ◽  
Simple Sequence ◽  
Selection Of ◽  
Russian Research

Studying of sunflower lines resistant to broomrape race G, bred in V.S. Pustovoit All-Russian Research Institute of Oil Crops (VNIIMK), Krasnodar showed the genetic control of resistance is monogenic with incomplete dominance. The purpose of the research was to seek and analyze molecular markers for the genes of resistance to broomrape race G using PCRmethods. In our research we used six developed in VNIIMK lines-donors resistant to broomrape race G: RGP1, RGP2, RGB, RGL1, RGL2, RGA, and susceptible lines VK 101, VK 678, VK 680. We extracted DNA from the leaves of young sunflower plants using STAB-buffer. For PCR-analysis we used 17 pairs of primers of two types: SCAR (sequence characterized amplified region) and SSR (simple sequence repeat). Conditions of amplification are as recommended by authors of markers with experimental selection of a temperature for primers hybridization. Within the research we balanced involved into work SSR- and 12 SCAR-loci using BLAST and a set of HanXRQr1.0. Due to these data we composed a physical map of loci location. After assessment of six resistant and three susceptible parental lines, seven markers demonstrated polymorphism by a length of locus DNA and two ones – by presence/absence of amplified fragment. For markers ORS 683 and ORS 1112 we observed a relation between loci polymorphism and susceptibility of parental lines to broomrape. This was certified with a presence of an allele of 364 n.p. length in ORS 683 and an absence of an allele of 375 n.p. length in ORS 1112. These markers were noted as primary ones for hybridological analysis in F1 и F2 with selected pairs for crossing. Additional markers will be selected individually using obtained data on loci polymorphism. Thus, as a result of the research we created a physical map for the further markers selection, found hybrid combinations and markers from the studied ones for hybridological analysis.

scholarly journals The evaluation of collection variety samples of menthol mint (Mentha L.) for disease resistance

2020 ◽  
Vol 4 (184) ◽  
pp. 63-70
Author(s):  
G.M. Saenko ◽  
◽  
T.P. Shuvaeva ◽  
I.V. Gaygotina ◽  
◽  
...  
Keyword(s):  
Disease Resistance ◽  
Powdery Mildew ◽  
Essential Oil ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Parental Material ◽  
Western Ciscaucasia ◽  
Russian Research ◽  
Research Institute

It is necessary to evaluate the variety samples of the crop to obtain the parental material of menthol mints, which will have a high content of essential oil, and be less affected by various diseases. We evaluated the variety samples of mint for disease resistance at the Voznesenskaya branch of V.S. Pustovoit of All-Russian Research Institute of Oil Crops. In the conditions of the branch in the piedmont area of the Western Ciscaucasia, there were identified diseases such as powdery mildew, rust, anthracnose and Fusarium blight. The studied variety samples were characterized by varying degrees of resistance to these diseases. The most harmful disease for mint is rust, the pathogens of which are the Puccinia menthae Pers. and P. angustata Peck. We identified the parental material for development a variety of peppermint Rozovskaya Aroma by analyzing the variety samples of menthol mint for three years of vegetation. Also, we developed and applied the modified scale for evaluation of resistance to the main diseases for the menthol forms of mint.

scholarly journals Highly oleic collection of flax in VNIIMK

2021 ◽  
Vol 186 (2) ◽  
pp. 46-49
Author(s):  
S.V. Sklyarov ◽  
◽  
L.G. Ryabenko ◽  
V.S. Zelentsov ◽  
L.R. Ovcharova ◽  
...  
Keyword(s):  
Oil Content ◽  
Weather Conditions ◽  
Good Combination ◽  
Biological Traits ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Two Samples ◽  
Economically Valuable Traits ◽  
Russian Research ◽  
Research Institute

The researches were conducted in the laboratory of oil flax breeding of the V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar 2019– 2020. We studied the samples from the highly oleic collection of flax of the V.S. Pustovoit All-Russian Research Institute of Oil Crops in order to select the best ones for introduction into a breeding process to improve the existing varieties and develop new ones with a good combination of biological and economically valuable traits. The samples from our collection demonstrated variability by the general economically valuable and biological traits. Thus, difference in duration of a period emergence–maturing was from 10 to 12 days depending on year and weather conditions. Maximal plant height (63.3 сm) was noted in 2020, and minimal (38.0 cm) was similar in all years of the research. Seed yield per a plot varied from 32.5 to 112.5 g/m 2 in 2019 and from 30.0 to 175.0 g/m 2 in 2020. The highest yield (175.0 g/m2) was fixed in 2020, and minimal one (30.0 g/m2) was at the same level in all years of the research. The samples from the collection were differed by their oil content in seeds: from 44.6 to 48.7% in 2019 and from 44.8 to 49.3% in 2020. Variation of this trait by years was 4.1–4.5%, difference between years of the research was equal to 0.4%. The presence of variability in oil contents in seeds among samples from flax collection proves a possibility to conduct selections by this trait in all breeding nurseries of the laboratory. Thus, in 2020 we selected two samples with oil content in seeds more than 49.0%.

scholarly journals Methodic aspects of selection of self-fertile biotypes in production of foundation seeds of sunflower varieties

2020 ◽  
Vol 183 (3) ◽  
pp. 3-10
Author(s):  
A.D. Bochkovoy ◽  
◽  
V.A. Kamardin ◽  
D.A. Nazarov ◽  
◽  
...  
Keyword(s):  
Main Part ◽  
Rating Scale ◽  
The Other ◽  
Fertility Level ◽  
Oil Crops ◽  
Rostov Region ◽  
Russian Research Institute ◽  
Economically Valuable Traits ◽  
Selection Of ◽  
Russian Research

In 2017–2019 we conducted researches in NPO “Triumph”, Matveevo-Kurgansky district, Rostov region, and in fields of V.S. Pustovoit All-Russian Research Institute of Oil Crops, Krasnodar. The purpose of the research was to develop methodic questions of selection of self-fertile biotypes during production of foundation seeds of confectionary sunflower varieties. We stated self-sterile and semisterile consist the main part of the studied seven OPvarieties and are equal 68.5% in average. They vary from 60.3% in the Enisey variety to 75.0% in Dobrynya. Basing on our researches we proposed a rating scale for self-fertility level in sunflower varieties and a scheme of foundation seeds growing oriented for selection of biotypes with increased selffertility. We developed a promising varietypopulation Dobrynya+, its average self-fertility of plants is equal to 12.2% comparing to 6.5% of the initial variety. A share of the less valuable self-sterile biotypes in it decreased from 22.2 to 12.0%, and a portion of the most valuable highly self-fertile, contrariwise, increased from 13.9 to 18.0%. By a complex of the other economically valuable traits a population Dobrynya+ does not differ of the initial variety.

scholarly journals THE STUDY OF BREEDING CHARACTERISTICS OF SULFONYLUREA-RESISTANT POPULATION FAMILIES OF DZHINN-SUR IN SUNFLOWER

2021 ◽  
Author(s):  
N.V. Ryabovol ◽  
◽  
A.A. Pikhtyaryova ◽  
Keyword(s):  
Field Experiment ◽  
Herbicide Resistance ◽  
Seed Weight ◽  
Oil Content ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Resistant Population ◽  
Field And Laboratory Conditions ◽  
Thousand Seed Weight ◽  
Russian Research

The article is dedicated to the study of sulfonylurea-resistant population families of Dzhinnsur for their further use as a new breeding material. We carried out the experiments in field and laboratory conditions at V.S. Pustotovoit All-Russian Research Institute of Oil Crops (Krasnodar) in 2020. In the field experiment we treated the plants with the herbicide Express and we evaluated the plants by a scale of phytotoxicity in all population families of Dzhinn-sur. We the studied population families by the following characteristics: thousand-seed weight, huskness, oil content and linear size. As a result of this work, we selected four population families of Dzhinn-sur, which are constant in their herbicide resistance; they will be used in further breeding work.

scholarly journals Broomrape-resistant sunflower hybrid Taizar

2020 ◽  
Vol 184 (4) ◽  
pp. 87-90
Author(s):  
Ya.N. Demurin ◽  
◽  
V.D. Savchenko ◽  
O.M. Borisenko ◽  
A.N. Levutskaya ◽  
...  
Keyword(s):  
Target Gene ◽  
High Yield ◽  
Early Group ◽  
Genetic Purity ◽  
Morphological Marker ◽  
Oil Crops ◽  
Russian Research Institute ◽  
Resistant Genotypes ◽  
Selection Of ◽  
Russian Research

A simple sunflower hybrid Taizar was developed at V.S. Pustovoit All-Russian Research Institute of Oil Crops as part of a breeding and genetic program for developing highly productive hybrids resistant to the new virulent races of broomrape by using the method of backcrossing in combination with an evaluation of resistance in each generation for the target gene Or7. The hybrid Taizar is of mid-early group of ripeness, it has a high yield, it is resistant to Orobanche cumana Wallr. (races A-G) and downy mildew (races 330, 710, 730, 334, 734). The line-restorer of pollen fertility VK 305 is the paternal form of the hybrid Taizar. The line was developed as a result of the introduction of the Or7 gene from the RG line by two backcrosses into the line VK 303, subsequent inbreeding and selection of broomrape-resistant genotypes. The line VK 305 has narrow widely spaced ray flowers strongly curved towards the back of the head, which can serve as a morphological marker of genetic purity.

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