scholarly journals Comparative evaluation of 5% sodium hypochlorite and Nd:YAG laser in reducing endotoxins level from infected root canals – An in vivo microbiological study

2021 ◽  
Vol 4 ◽  
pp. 2021
Author(s):  
Aseem Prakash Tikku ◽  
Promila Verma ◽  
Ramesh Bharti ◽  
Prashant Gupta ◽  
Brijesh Singh
Keyword(s):  
Calcium Hydroxide ◽  
Sodium Hypochlorite ◽  
Nd:Yag Laser ◽  
Dental Treatment ◽  
Bacterial Species ◽  
Control Group ◽  
Root Canals ◽  
Endodontic Infection ◽  
Infected Root ◽  
Experimental Group

Objectives: Endodontic infection is one of the most challenging aspects of dental treatment which is primarily caused by Gram-negative anaerobic bacterial species. The primary objective in the management of endodontic treatment is eradication of bacteria, as well as elimination of endotoxin. The aim of this study was to compare the effectiveness of 5% sodium hypochlorite, a mixture of calcium hydroxide+2% chlorhexidine (CHX) gel and Nd:YAG lasers for decreasing the level of endotoxin in infected root canals. Material and Methods: Forty four patients in the age group of 18–50 years were carefully chosen for the study and divided into control (Group 1, n = 11) and experimental group (Group 2, n = 33). Experimental group was further split into three subgroups according to the medicaments used. Samples were collected after the access opening (pre-operative specimens), after the biomechanical preparation (intermediate samples), and after the application of intracanal medicament for 15 days. The concentration of endotoxin in each sample was measured using quantitative chromogenic limulus amebocyte lysate assay. The data collected were statistically analyzed using SPSS for Windows (Statistical Package for the Social Sciences, Inc., Chicago, IL,) Version 15.0 Statistical Analysis Software. The level of significance was set at 0.05 for all tests. Results: Pretreatment endotoxins level observed was 6.783EU/ml in Control group and 7.261EU/ml, 6.963EU/ml, 7.247EU/ml in experimental subgroups. After biomechanical preparation and use of medicaments endotoxins level reduced to 3.919EU/ml in Control group and 0.5222EU/ml (sodium hypochlorite) 1.164EU/ml (calcium hydroxide+chlorhexidine), 0.841EU/ml (laser). Conclusion: This study concluded after analyzing the data that use of 5% sodium hypochlorite and Nd:YAG laser was effective in decreasing the level of endotoxins and use of intracanal medicament (2% CHX+ calcium hydroxide) for 15 days further helps to reduce the endotoxin level.

scholarly journals COMPARATIVE EVALUATION OF ROOT CANAL DISINFECTION USING 3% SODIUM HYPOCHLORITE, 940NM DIODE LASER AND PHOTODYNAMIC THERAPY IN SINGLE SITTING PULPECTOMY: AN IN-VIVO STUDY

2020 ◽  
pp. 1-3
Author(s):  
Thomas T Parakkal ◽  
Pulkit Jhingan ◽  
Shivani Mathur ◽  
Vinod Sachdev
Keyword(s):  
Photodynamic Therapy ◽  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Bacterial Species ◽  
Root Canals ◽  
Root Canal Disinfection ◽  
Infected Root ◽  
Colony Forming Units

Introduction: Enterococcus faecalis is a common bacterial species in resistant and recurrent infections. It has an active proton pump which prevents its easy inhibition leading to endodontic treatment failure. LASER and photodynamic therapy have been employed lately in improving antimicrobial chemotherapy of localized infections. Aim: To compare disinfection efficacy of 940nm Diode LASER & Photodynamic therapy(PDT) to Conventional method using 3% Sodium hypochlorite in canals of endodontically treated primary teeth. Method: Single sitting Pulpectomy was performed on 60 mandibular first primary molars, divided into 3 groups, disinfected using mentioned modalities. Disinfection potential was compared collecting samples at 3 stages using paper points, cultured and incubated for 24hrs to check change in Colony Forming Units of the bacteria. Results: Data analysis with SPSS version 20 software using Kruskal-Wallis and Mann Whitney-U test. Conclusion: The three groups showed effective disinfection as revealed by the reduction in the colony forming units of the bacteria at baseline and after intervention. However, diode laser when used in conjunction with sodium hypochlorite as a disinfecting modality was found to be the most effective for the eradication of Enterococcus faecalis from infected root canals.

scholarly journals Intracanal Irrigating Solutions Prior to Calcium Hydroxide Medication and Its Effects on Root Dentin Strength

2017 ◽  
Vol 28 (1) ◽  
pp. 46-50
Author(s):  
Leila Clarisse Hillesheim ◽  
Juliara Bellina Hoffmann ◽  
Daniela Peressoni Vieira Schuldt ◽  
Maybell Tedesco ◽  
Eduardo Antunes Bortoluzzi ◽  
...  
Keyword(s):  
Compressive Strength ◽  
Sodium Chloride ◽  
Calcium Hydroxide ◽  
Sodium Hypochlorite ◽  
Control Group ◽  
Compressive Test ◽  
Crosshead Speed ◽  
Root Canals ◽  
Ethylenediaminetetracetic Acid ◽  
Root Dentin

Abstract The aim of this study was to assess the effects of intracanal irrigation with sodium hypochlorite (NaOCl) and ethylenediaminetetracetic acid (EDTA) prior to calcium hydroxide medication on the compressive strength of root dentin. Forty-eight roots with single and straight canals were instrumented with a crown-down technique using Flexofile files and Gates Glidden burs, under 0.9% sodium chloride (NaCl) irrigation. Root canals were randomly separated into 4 groups (n=12), according to the irrigating solution: Control Group, 0.9% NaCl; GroupNaOCl, 1% NaOCl; GroupEDTA, 17% EDTA; and GroupEDTA+NaOCl, 17% EDTA and 1% NaOCl. Solutions remained for 40 min, renewed every 10 min, except for GroupEDTA+NaOCl, where the solutions were alternated every 10 min. Canals were dried and filled with calcium hydroxide paste, and radiographed. Each canal was sealed and stored at 37 °C for 30 days. After this period, the roots were cut 1 mm below the cementoenamel junction and 6 mm apically from this point in order to obtain 6 mm-long cylinders. The compressive test was conducted with a crosshead speed of 1 mm/min. The means (±SD) (MPa) of compressive strength were: Control Group, 151.23±94.36; GroupNaOCl, 167.50±57.25; GroupEDTA, 108.79±99.88; GroupEDTA+NaOCl, 60.12±92.10. Significant statistical differences among groups were found (ANOVA and Tukey, a=5%, p=0.02). Control Group and GroupNaOCl showed higher means of compressive strength than GroupEDTA+NaOCl. Intracanal irrigation with 17% EDTA and 1% NaOCl produced a significant reduction of dentin compressive strength 30 days after medication with calcium hydroxide.

scholarly journals Prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals and their susceptibility to endodontic treatment procedures: A molecular study

2014 ◽  
Vol 142 (9-10) ◽  
pp. 535-541 ◽  
Author(s):  
Nikola Stojanovic ◽  
Jelena Krunic ◽  
Branka Popovic ◽  
Sonja Stojicic ◽  
Slavoljub Zivkovic
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Porphyromonas Gingivalis ◽  
Molecular Study ◽  
Apical Periodontitis ◽  
Pcr Assay ◽  
Root Canals ◽  
Endodontic Infection ◽  
Infected Root ◽  
Significant Difference

Introduction. Because apical periodontitis is recognizably an infectious disease, elimination or reduction of intracanal bacteria is of utmost importance for optimum treatment outcome. Objective. The prevalence of Enterococcus faecalis and Porphyromonas gingivalis in infected root canals was studied Also, the effect of endodontic therapy by using intracanal medicaments, calcium hydroxide paste (CH) or gutta-percha points containing calcium hydroxide (CH-GP) or chlorhexidine (CHX-GP) on these microorganisms was assessed by polymerase chain reaction (PCR) assay. Methods. Fifty-one patients with chronic apical periodontitis were randomly allocated in one of the following groups according to the intracanal medicament used: CH, CH-GP and CHX-GP group. Bacterial samples were taken upon access (S1), after chemomechanical instrumentation (S2) and after 15-day medication (S3). PCR assay was used to detect the presence of selected bacteria. Results. E. faecalis was detected in 49% (25/51) and P. gingivalis in 17.6% (9/51) of the samples. Samples which showed no bacterial presence at S1 were excluded from further analysis. Overall analysis of all 29 samples revealed significant differences between S1 and S2 (p<0.001), S2 and S3 (p<0.05), and S1 and S3 (p<0.001). When distinction was made between the intracanal medications, there was a significant difference in the number of PCR positive samples between S1 and S2, S1 and S3, but not between S2 and S3 samples. Conclusion. E. faecalis is more prevalent than P. gingivalis in primary endodontic infection. Intracanal medication in conduction with instrumentation and irrigation efficiently eliminates E. faecalis and P. gingivalis from infected root canals.

Antimicrobial Effectiveness of Photodynamic Therapy, 5% Sodium Hypochlorite and 2% Chlorhexidine Gluconate in Root Canal Treated Teeth: A Clinical Study

2021 ◽  
pp. 232020682110189
Author(s):  
Ramesh Bharti ◽  
Aseem Prakash Tikku ◽  
Anil Chandra ◽  
Prashant Gupta
Keyword(s):  
Photodynamic Therapy ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Bacterial Species ◽  
Bacterial Count ◽  
Chlorhexidine Gluconate ◽  
Total Bacterial Count ◽  
Root Canals ◽  
Anterior Teeth ◽  
Infected Root

Objective: To evaluate the effectiveness of photodynamic therapy (PDT), sodium hypochlorite (NaOCl) and chlorhexidine gluconate (CHO) in the endodontic re-treatment in the removing of microorganisms from previously filled infected root canals. Materials and Methods: The study samples consist of 54 root canal treated anterior teeth with apical periodontitis were randomly divided into three groups according to the disinfection protocol. Microbiological samples from the root canals were collected after removing the gutta-percha (S1) and following the endodontic re-treatment (S2) with either 5% NaOCl, 2% CHO or PDT procedure for 1 minute. Microbiological samples were cultivated on selective plates to assess the presence or absence of microbial growth and determine the average reduction of viable microorganisms. Results: Significant reduction in the total bacterial count in all the groups at post treatment ( P < .001). PDT provided statistically higher (99.74%) elimination of Enterococcus faecalis in comparison to NaOCl (95%) and CHO (88%). Conclusions: The use of PDT in endodontic re-treatment led to the significant reduction of the remaining bacterial species. So, PDT can be used for routine endodontic disinfection in failed root canal cases.

scholarly journals Passive ultrasonic irrigation in calcium hydroxide removal from root canals: a sem/eds analysis

2015 ◽  
Vol 3 (3) ◽  
pp. 668
Author(s):  
Fernanda Hoffmann Busanello ◽  
Angela Longo Do Nascimento ◽  
Lilian Tietz ◽  
Mirela Sangoi Barreto ◽  
Ricardo Abreu Da Rosa ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Root Canal ◽  
Control Group ◽  
Passive Ultrasonic Irrigation ◽  
Root Canals ◽  
Eds Analysis ◽  
Ultrasonic Irrigation ◽  
Calcium Hydroxide Removal ◽  
And Control ◽  
Elementary Chemical

AIM: The aim of this study was to evaluate the efficacy of different lengths of time of passive ultrasonic irrigation (PUI) in removing calcium hydroxide (CH) paste from root canal, using scanning electron microscopy and energy dispersive spectrometry (SEM/EDS). MATERIAL AND METHODS: Fifty-four human premolars were used. After coronal access, a size #15 K-file was used to obtain apical patency. Root canal preparation was performed using WaveOne 40.08 instruments. CH paste was placed into the root canal. Specimens were stored in a humid environment, and after seven days, they were divided into five groups (n=12) according to the irrigation protocols: Manual– a size #40 K-file; PUI/1– 1 min; PUI/2– 2 min; PUI/3– 3 min; and Control– without CH paste. An elementary chemical microanalysis (SEM/EDS) was performed to quantify the presence of calcium on the dentinal walls. RESULTS: The percentage of calcium was higher in all experimental groups when compared to the control group (P < 0.05); but no differences among them were found (P > 0.05). CONCLUSION: The amount of calcium hydroxide paste on the dentinal walls was not dependent on length of time of ultrasonic activation. SEM/EDS analysis seems to be a reliable method to assess CH paste removal from the root canals.

scholarly journals Effectiveness of calcium hydroxide in reducing bacterial load comparing with chemo-mechanical preparation- A systematic review

2019 ◽  
Author(s):  
SaiSiva Naga GayathriNaidu ◽  
B Vikram Simha ◽  
Koka Krishna Mohan ◽  
Rammohan Modem ◽  
Kommineni Harish Chowdary
Keyword(s):  
Clinical Trials ◽  
Calcium Hydroxide ◽  
Bacterial Load ◽  
Bacterial Reduction ◽  
Bacterial Populations ◽  
Hand Search ◽  
Root Canals ◽  
Mechanical Preparation ◽  
Infected Root ◽  
Significant Difference

Aim: To determine and compare to what extent the intra-canal medicament like calcium hydroxide along with chemo-mechanical preparation reduces the bacterial load in infected root canals. Methodology: PubMed Cental, Medline, Google Scholar and hand search was carried out using the keywords calcium hydroxide (Ca(OH)2) , intra-canal medicament(ICM) and chemo-mechanical preparation. Selection Criteria: Clinical trials in which bacterial samples were evaluated at 3 intervals (Baseline, after chemo-mechanical preparation, after dressing with intra canal medicament ICM and trials which use Ca(OH)2 with any vehicle as ICM, but not compared with any other ICM were selected for this review. The data collection form was customized. The primary outcome measures were reduction of bacterial populations in infected root canals. Results: Seven clinical trials that met all inclusion criteria involving 117 participants were reviewed. Included studies showed significant difference in bacterial reduction after chemo-mechanical preparation and placement of Ca(OH)2 dressing for a week. In all included trials, Ca(OH)2 reduced bacterial load further compared with chemo-mechanical preparation. Conclusion: Ca(OH)2 as an inter-appointment medication promoted further decrease in the bacterial bioburden to levels significantly below those achieved by the chemo-mechanical procedures alone. Intra-canal medicament such as Ca(OH)2 should be considered for bacterial reduction along with chemo-mechanical preparation for long term success. Key Words: Intra-canal medicament, calcium hydroxide, Root canal treatment, bacterial load, Pulpitis

scholarly journals Mechanism of action of sodium hypochlorite

2002 ◽  
Vol 13 (2) ◽  
pp. 113-117 ◽  
Author(s):  
Carlos Estrela ◽  
Cyntia R.A. Estrela ◽  
Eduardo Luis Barbin ◽  
Júlio César E. Spanó ◽  
Melissa A. Marchesan ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Mechanism Of Action ◽  
Chemical Properties ◽  
Cellular Metabolism ◽  
Previous Knowledge ◽  
Host Defenses ◽  
Root Canals ◽  
Physico Chemical ◽  
Infected Root ◽  
Enzymatic Inactivation

The choice of an irrigating solution for use in infected root canals requires previous knowledge of the microorganisms responsible for the infectious process as well as the properties of different irrigating solutions. Complex internal anatomy, host defenses and microorganism virulence are important factors in the treatment of teeth with asymptomatic apical periodontitis. Irrigating solutions must have expressive antimicrobial action and tissue dissolution capacity. Sodium hypochlorite is the most used irrigating solution in endodontics, because its mechanism of action causes biosynthetic alterations in cellular metabolism and phospholipid destruction, formation of chloramines that interfere in cellular metabolism, oxidative action with irreversible enzymatic inactivation in bacteria, and lipid and fatty acid degradation. The aim of this work is to discuss the mechanism of action of sodium hypochlorite based on its antimicrobial and physico-chemical properties.

scholarly journals Periradicular repair after two-visit endodontic treatment using two different intracanal medications compared to single-visit endodontic treatment

2007 ◽  
Vol 18 (4) ◽  
pp. 299-304 ◽  
Author(s):  
Adriana M. Vieira Silveira ◽  
Hélio P. Lopes ◽  
José F. Siqueira Jr ◽  
Sérgio B. Macedo ◽  
Alberto Consolaro
Keyword(s):  
Success Rate ◽  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Endodontic Treatment ◽  
Controversial Issues ◽  
Single Visit ◽  
Root Canals ◽  
Infected Root

The number of appointments necessary to treat infected root canals is one of the most controversial issues in endodontics. This study evaluated, in dogs, the response of the periradicular tissues to the endodontic treatment of infected root canals performed in a single visit or in two visits, using different interappointment dressings. Periradicular lesions were induced by inoculating Enterococcus faecalis in the root canals. After confirming that a periradicular lesion developed, the root canals were treated within one or two visits, using either ozonized oil or calcium hydroxide in camphorated paramonochlorophenol (CMCP) as an intracanal medication. After 6 months, the animals were sacrificed and the specimens were processed for histological and histobacteriological analysis. The root canals treated in a single visit showed a success rate of 46%. When a calcium hydroxide/CMCP-based interappointment intracanal medication was used, 74% of the cases were categorized as success. In cases where ozonized oil was used as the intracanal medication, a success rate of 77% was observed. These results of the present study demonstrated that the two-visit treatment offered a higher success rate compared to one-visit therapy. In addition, ozonized oil may potentially be used as an intracanal medication.

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