scholarly journals PEMANFAATAN SLUDGE INSTALASI PENGOLAHAN AIR LIMBAH INDUSTRI PULP DAN KERTAS SEBAGAI BAHAN BAKU BIOETANOL

2016 ◽  
Vol 2 (02) ◽  
Author(s):  
Rina S. Soetopo ◽  
Sri Purwati ◽  
Yusup Setiawan ◽  
Susi Sugesty
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sugar Content ◽  
Treatment Plant ◽  
Pulp And Paper ◽  
Raw Material ◽  
Process Efficiency ◽  
Process Conditions ◽  
Primary Sludge ◽  
Hydrolysis Process ◽  
Virgin Pulp

Investigation on utilization of sludge waste from Wastewater Treatment Plant (WWTP) of pulp and paper mill for bioethanol feedstock in a batch system at laboratory scale has been carried out. Experiments were conducted in three phases, namely the hydrolysis process; the fermentation process by Saccharomyces cerevisiae and the simultaneous saccharification-fermentation experiments. Ethanol concentration produced was tested by the HPLC method. Results show that t primary sludge from paper mills made from virgin pulp (sludge A) and primary sludge from pulp and paper made from raw wood (sludge B) at a 2% total solids having potential as raw material for bioethanol. The optimum conditions of the hydrolysis process of sludge A and sludge B is obtained from the sludge solids content of 6%, with the addition of cellulase of 9 FPU/g cellulose and beta-glucosidase of 6.7 IU/FPU for the incubation time of 48 hours producing the reducing sugar content of 31.3% and 36.2% with the hydrolysis efficiency of 64% and 71% respectively. On the SSF process conditions at pH of 4.5, temperature of 28oC for 96 hours, the sludge substrate A can produce ethanol with the concentration of 3.45% and the process efficiency of 72.5% and the sludge substrate B can produce ethanol with the concentration of 2.89% and the process efficiency of 60.8%.Keywords: bioethanol, cellulase, hydrolysis, fermentation, Saccharomyces cerevisiae, sludge  ABSTRAK Penelitian pemanfaatan sludge Instalasi Pengolahan Air Limbah (IPAL) industri pulp dan kertas untuk bahan baku bioetanol dengan sistem batch pada skala laboratorium telah dilakukan. Percobaan dilakukan dalam tiga tahapan yaitu proses hidrolisis dengan enzim; proses fermentasi dengan Saccharomyces cerevisiae dan proses sakarifikasi-fermentasi serentak (SFS) pada satu reaktor. Kadar etanol yang dihasilkan diuji dengan metode HPLC. Hasil percobaan menunjukkan bahwa dari 4 jenis sludge yang diteliti, sludge primer dari pabrik kertas berbahan baku virgin pulp (sludge A) dan dari pabrik pulp dan kertas berbahan baku kayu (sludge B) berpotensi sebagai bahan baku bioetanol. Kondisi optimum percobaan proses hidrolisis sludge A dan sludge B diperoleh dari perlakuan dengan kadar padatan 6%, dosis selulase 9 FPU/g selulosa; dosis beta glukosidase 6,7 IU/FPU dengan lama inkubasi 48 jam, masing-masing menghasilkan kadar gula pereduksi sebesar 31,3% dan 36,2% dengan efisiensi hidrolisis 64% dan 71%. Pada percobaan SFS dengan kondisi proses pH 4,5 pada suhu 28oC selama 96 jam, substrat sludge A dapat menghasilkan etanol sebesar 3,45% dengan efisiensi proses 72,5% dan substrat sludge B dapat menghasilkan etanol sebesar 2,89% dengan efisiensi proses 60,8%.Kata kunci: bioetanol, selulase, hidrolisis, fermentasi, Saccharomyces cerevisiae, sludge

scholarly journals LUMPUR PRIMER IPAL INDUSTRI KERTAS SEBAGAI BAHAN BAKU ALTERNATIF BIOETANOL

2016 ◽  
Vol 2 (01) ◽  
Author(s):  
Saepulloh , ◽  
Rina S. Soetopo ◽  
Prima Besty Asthary
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Yield ◽  
Waste Water Treatment ◽  
Treatment Plant ◽  
Raw Material ◽  
Waste Water Treatment Plant ◽  
Main Stage ◽  
Glucose Fermentation ◽  
Glucose Content ◽  
Primary Sludge

Primary sludge from paper mill waste water treatment plant (WWTP) is a potential raw material for ethanol production because of its high organic content especially cellulose. This study consisted of two main stage: cellulose hydrolisis and glucose fermentation. Primary sludge was hydrolyzed using cellulase with concentration of 10, 20, and 25 FPU/g cellulose under conditions of pH 5.5 and temperature 50oC for 24, 48, 72, and 98 hours. Glucose obtained from hydrolis was fermented in optimum condition (pH 4.5 and temperature 28oC) using 10% of Saccharomyces cerevisiae inoculum for 24, 48, 72, and 98 hours. The results showed that the optimum hydrolysis was achieved using cellulase 25 FPU within 72 hours with glucose content obtained was 1.92% and saccharification degree was 61.05%. Ethanol produced from glucose fermentation was about 0.77% and fermentation degree was 78.5%. Overall conversion yield was 46.61% and ethanol yield was 192.5 g/kg dry weight.Keywords: papermill primary sludge,cellulose, cellulase, Saccharomyces cerevisiae, bioethanol  ABSTRAKLumpur primer dari IPAL industri kertas mempunyai potensi sebagai bahan baku untuk produksi bioetanol, karena kandungan organiknya yang tinggi terutama selulosa. Lumpur primer IPAL diperoleh dari industri kertas berbahan baku virgin pulp. Secara garis besar percobaan dilakukan dengan sistem dua tahap yaitu proses hidrolisis selulosa dan dilanjut proses fermentasi glukosa. Percobaan proses hidrolisis selulosa dilakukan pada pH 5,5 dan suhu 50oC dengan 2 faktor perlakuan yaitu dosis selulase (10, 20, dan 25 FPU/g selulosa) dan lama proses hidrolisis (24, 48, 72, dan 96 jam), sedangkan percobaan proses fermentasi dilakukan terhadap glukosa hasil proses hidrolis pada kondisi optimumnya. Proses fermentasi dilakukan pada pH 4,5 dan suhu 28oC menggunakan ragi Saccharomyces cerevisiae sebanyak 10% dengan perlakuan lama inkubasi (24; 48; 72; 96 jam). Hasil percobaan menunjukkan bahwa kondisi optimum proses hidrolisis diperoleh pada dosis selulase 25 FPU/g selulosa selama 72 jam, dengan kadar glukosa 1,92% dan derajat sakarifikasi 61,05%. Kadar etanol yang diperoleh dari proses fermentasi glukosa hasil hidrolisis adalah 0,77% dengan efisiensi fermentasi 78,5%. Secara keseluruhan efisiensi konversi selulosa pada lumpur primer IPAL industri kertas menjadi etanol adalah 46,61% dengan yield sebesar 192 g/kg berat kering.Kata kunci: lumpur primer industri kertas, selulosa, selulase, Saccharomyces cerevisiae, bioetanol

scholarly journals Pemanfaatan Lumpur Primer Industri Kertas sebagai Absorben

2018 ◽  
Vol 8 (01) ◽  
pp. 9
Author(s):  
Krisna Adhitya Wardhana ◽  
Saepulloh Saepulloh ◽  
Reynaldo Biantoro
Keyword(s):  
Performance Test ◽  
Cellulose Fiber ◽  
Paper Mill ◽  
Absorption Capacity ◽  
Raw Material ◽  
Paper Mills ◽  
Primary Sludge ◽  
Increase Surface Area ◽  
Absorption Performance ◽  
Virgin Pulp

The Utilization of Primary Sludge from Paper Mills as AbsorbentAbstractThe primary sludge from paper mill contains cellulose fiber that has high absorption capacity and potential to be used as an absorbent for hydrophobic compounds. The utilization of primary sludge from paper mill using raw material of waste paper and virgin pulp to be used as an absorbent has been conducted. The stages process of absorbent fabrication was consisted of three stages such as drying, mechanical and chemical processes. Mechanical process conducted were crushing and grinding methods to increase surface area and pore volume of sludge. Meanwhile silanization process as chemical treatment using solution of γ-metacryloxypropyl trimethoxysilane in ethanol to improve the hydrophobic properties of primary sludge was also done. Absorbent products were tested for the maximum sorbency, the sorbent performance, absorption of hydrophobic liquid, absorption of hydrophobic dust, and contact angle test to conduct their performance. The absorbency of absorbent product which is covered by cushion material was also tested. Result indicated that the absorption performance of some absorbent product is higher than commercial absorbent on hydrophobic liquid and hydrophobic dust absorption test. But it was lower than commercial absorbent on maximum sorbency and the sorbent performance test. The use of cushion material as a wrapper could make it easy in the collection and could increase the absorbency.Keywords: primary sludge; mechanical; silanization; absorbent; hydrophobic AbstrakLumpur primer dari industri kertas mengandung serat selulosa yang memiliki daya serap tinggi dan memiliki potensi dimanfaatkan sebagai absorben senyawa hidrofobik. Pemanfaatan lumpur primer industri kertas berbahan baku kertas bekas dan virgin pulp sebagai absorben telah dilakukan. Tahapan proses pembuatan absorben dari lumpur primer meliputi proses pengeringan, proses mekanis, dan proses kimia. Proses mekanis yang dilakukan adalah crushing dan grinding untuk meningkatkan luas permukaan dan volume pori-pori lumpur dilanjutkan dengan proses silanisasi (silanization) sebagai perlakuan kimia menggunakan larutan γ-metacryloxypropyl trimethoxysilane dalam etanol untuk meningkatkan sifat hidrofobik lumpur. Produk absorben diuji daya serap maksimum (maximum sorbency), kinerja absorben, penyerapan cairan hidrofobik, penyerapan debu hidrofobik, dan pengujian sudut kontak. Daya serap produk absorben yang ditutup bahan bahan cushion juga diuji. Hasil menunjukkan kemampuan absorbsi cairan hidrofobik dan debu hidrofobik dari beberapa perlakuan produk absorben lebih tinggi dari absorben komersial, sedangkan hasil maximum sorbency dan uji kinerja absorben menunjukkan hasil lebih rendah dari absorben komersial. Penggunaan bahan cushion sebagai pembungkus dapat memudahkan dalam pengumpulan dan dapat meningkatkan penyerapan.Kata kunci: lumpur primer; mekanis; silanisasi; absorben; hidrofobik 

scholarly journals Design Process of Hydrolysis and Fermentation Bioethanol Production from Seaweed Eucheuma cottonii to Renewable Energy Sovereignity

2016 ◽  
Vol 3 (3) ◽  
pp. 107
Author(s):  
Wagiman . ◽  
Makhmudun Ainuri ◽  
Rinda Gusvita ◽  
Jumeri .
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Process Design ◽  
Sugar Content ◽  
Reducing Sugar ◽  
Bioethanol Production ◽  
Fermentation Efficiency ◽  
Hydrolysis Process ◽  
High Reduction ◽  
Eucheuma Cottonii ◽  
Fermentation Substrate

<p>The aim of this research was study of E. cottonii to produce bioethanol fermentation substrate with a high reduction sugar content and low Hidroxymethilfurfural (HMF). Fermentation done by instant yeast and Saccharomyces cerevisiae culture of FNCC 3012.The best treatment was obtained in the combination of 2% of H2SO4 by time reaction of 120 minutes in 80°C produced 15.61 g/l reducing sugar and 5.03 g/l HMF. In fermented process, the hydrolysate with instant yeast starter delivered much more efficiency in 3.63 ml CO2 volume, 87.53% in fermentation efficiency, and 1.96 g/l reducing sugar on fifth day of fermentation. <br /><strong>Keywords</strong>: bioethanol, Eucheuma cottonii, fermentation, hydrolysis, process design</p>

scholarly journals BIOETHANOL PRODUCTION FROM COCONUT HUSK USING THE WET GAMMA IRADIATION METHOD

2021 ◽  
Vol 14 (2) ◽  
pp. 43
Author(s):  
Putra Oktavianto ◽  
Risdiyana Setiawan ◽  
Ilhami Ariyanti ◽  
Muhammad Fadhil Jamil
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cellulose Hydrolysis ◽  
Raw Material ◽  
Bioethanol Production ◽  
Coconut Husk ◽  
Hydrolysis Process ◽  
Hydrolysis Of Cellulose ◽  
Ethanol Ethanol ◽  
Hydrolysis Of ◽  
Low Dosage

BIOETHANOL PRODUCTION FROM COCONUT HUSK USING the WET GAMMA IRRADIATION METHOD. The use of coconut husk has only been used as a material for making handicrafts such as ropes, brooms, mats, and others or just burned. The combustion of coconut husk can cause air pollution. In fact, coconut husk can be used as a raw material for bioethanol production so that the beneficial value of coconut husk will also increase. One way of bioethanol production from coconut husk is by irradiating the coconut husk. The coconut husk irradiation technique to be carried out in this study is the wet irradiation technique. Wet irradiation is carried out to accelerate the process of bioethanol production because at the time of irradiation, cellulose has been hydrolyzed and glucose has been formed so that it is more efficient in time and use of the material so that the cellulose hydrolysis process is not necessary. The coconut husk samples were wet because they were mixed with 4% NaOH and were irradiated using a gamma irradiator from STTN-BATAN Yogyakarta with a dose of 30 kGy and 50 kGy and 0 kGy (or without irradiation). Then the sample is fermented with the fungus Saccharomyces Cerevisiae from tape yeast to form ethanol. Ethanol is purified and then analyzed for concentrations using pycnometric and refractometric methods. The result is that the highest ethanol content is without irradiation (0 kGy), this is due to the low dosage used. However, the main point in this wet method research is evidence of hydrolysis of cellulose by the formation of gluoxane after irradiated wet coconut husk, and with Fehling A and B analysis, brown deposits are seen proving that glucose has been formed.

Why applying THP on waste activated sludge makes sense: Psyttalia – Athens case study

2019 ◽  
Vol 14 (4) ◽  
pp. 921-930 ◽  
Author(s):  
D. Zikakis ◽  
J. Chauzy ◽  
I. Droubogianni ◽  
A. Georgakopoulos
Keyword(s):  
Activated Sludge ◽  
Energy Use ◽  
Substantial Reduction ◽  
Treatment Plant ◽  
Cost Savings ◽  
Waste Activated Sludge ◽  
Primary Sludge ◽  
Hydrolysis Process ◽  
Sludge Drying

Abstract In order to improve the energy footprint of Psyttalia wastewater treatment plant (WWTP) in Athens, the application of a thermal hydrolysis process (THP) was preferred to the option of constructing additional digesters. Since August 2015, approximately half of the generated waste activated sludge (WAS) has been treated by a Cambi B6-4 system, while the thickened primary sludge (PS) is by-passing the THP and is mixed with the hydrolysed WAS before entering the 4 digesters. The 4 other conventional digesters have treated the remaining sludge. The dewaterability of the mixed digested sludge has significantly been improved from 22% dry solids (DS) before THP installation, up to 31% DS after THP installation. This is providing substantial reduction of energy use and cost savings at the sludge drying plant of Psyttalia. In addition, biogas generation and digester efficiency (VSR) have been increased.

The Effect of Type and Concentration Yeast with Fermentation Time and Liquifaction Variations on the Bioethanol Concentration Resulted by Sorgum Seeds with Hydrolysis and Fermentation Processes

2019 ◽  
Vol 16 (12) ◽  
pp. 5228-5232
Author(s):  
Kiagus A. Roni ◽  
Dorie Kartika ◽  
Hasyirullah Apriyadi ◽  
Netty Herawati
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fermentation Process ◽  
Rhizopus Oryzae ◽  
Process Variations ◽  
Raw Material ◽  
Fermentation Time ◽  
Hydrolysis Method ◽  
Hydrolysis Process ◽  
Starch Composition ◽  
Yeast Concentration

Sorghum is one of the plants that can be used as raw material for making bioethanol. Sorghum has seeds with a starch composition of 73.8%, which is potential as a raw material for making bioethanol. Sorghum starch can be converted into bioethanol through the hydrolysis process (the process of converting carbohydrates into glucose) which consists of liquefaction and saccharification processes and is followed by a fermentation process. The hydrolysis method is carried out enzymatically. In this study alpha amylase and gluco amylase enzymes were used with various types of yeast including Saccharomyces cerevisiae, Rhizopus oryzae, Acetobacter xylinum, Mucor sp, and Aspergilus niger which varied with liquefaction temperatures including 80, 85, 90, 95, and 100 °C. Obtained the most optimal yeast is Saccharomyces cerevisiae with an optimal temperature of 95 °C resulting in a bioethanol concentration of 4.3%. After getting the optimal yeast and temperature, the fermentation step of the two variables is used in the next step. In the fermentation process, variations of yeast concentration and duration of fermentation were used, the optimum yeast concentration was at 2.5% with 48 hours of fermentation resulting in bioethanol concentration of 11%.

scholarly journals Integrated bioconversion of pulp and paper primary sludge to second generation bioethanol using Saccharomyces cerevisiae ATCC 26602

2016 ◽  
Vol 220 ◽  
pp. 161-167 ◽  
Author(s):  
Cátia V.T. Mendes ◽  
Crispin H.G. Cruz ◽  
Diana F.N. Reis ◽  
M. Graça V.S. Carvalho ◽  
Jorge M.S. Rocha
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Second Generation ◽  
Pulp And Paper ◽  
Primary Sludge ◽  
Second Generation Bioethanol

Potensi Sludge dari Industri Kertas Sebagai Bahan Baku Chipboard

2016 ◽  
Vol 9 (1) ◽  
pp. 1-12
Author(s):  
Henggar Hardiani ◽  
Rina Masriani
Keyword(s):  
Paper Industry ◽  
Cellulose Fiber ◽  
Paper Mill ◽  
Pulp And Paper ◽  
Raw Material ◽  
Chemical Components ◽  
Pulp And Paper Mill ◽  
Spectra Analysis ◽  
Primary Sludge ◽  
Ft Ir

From the regulatory perspectives, pulp and paper mill sludge management as a environmental issue is significant. The restricted of imported recycle paper is a problem for the pulp and paper industry. Therefore a research was conducted to determine utilization potential of the WWTP sludge from paper industry in Indonesia as raw material for chipboard based on the characteristics of the sludge. The environmental aspects evaluation also has been cundected TCLP test and toxicology LD50 to determine whether the sludge waste category B3. The test results showed that the levels of alpha cellulose sludge is high (45-84%). Primary Sludge from virgin pulp contains high alpha cellulose (76-84%), ash content (3-14%), and the fines are quite low (30-34%). Potential of primary and final sludge to be used as raw material for chipboard. However, the primary sludge is generally recycled so that the final sludge are more preferable to be utilized. The results of FT-IR spectra analysis showed that the dominant chemical components in the sludge are cellulose. Fiber and fines content in line with the observation using SEM. Based on the results of the TCLP test and LD50, it is known that the sludge contains heavy metals, inorganic and organic are stable, so it is safe for the environment and can be utilized. Thus sludge has potential as a raw material for chipboard.ABSTRAKPengelolaan limbah sebagai isu lingkungan penting untuk dilakukan, terutama dari perspektif regulasi.  Adanya larangan impor kertas daur ulang, merupakan masalah bagi industri pulp dan kertas.  Oleh karena itu dilakukan penelitian untuk meneliti potensi pemanfaatan sludge IPAL dari industri kertas di Indonesia sebagai bahan baku pembuatan chipboard berdasarkan karakteristik sludge. Evaluasi terhadap aspek lingkungan berdasarkan uji karakteristik beracun TCLP dan uji toksikologi LD50 untuk mengetahui apakah sludge termasuk kategori limbah B3 juga dilakukan. Hasil uji menunjukkan bahwa  kadar alfa selulosa sludge relatif cukup tinggi (45-84%). Sludge primer pulp virgin mengandung kadar alfa selulosa yang tinggi (76-84 %), kadar abu (3-14 %), dan kadar fines yang cukup rendah (30-34 %). Sludge primer maupun sludge  final berpotensi untuk dimanfaatkan sebagai bahan baku pembuatan chipboard. Akan tetapi sludge primer pada umumnya didaur ulang sehingga sludge final lebih berpotensi untuk dimanfaatkan. Hasil analisa spektra FT-IR sludge menunjukkan bahwa komponen kimia yang dominan dalam sludge adalah selulosa. Data kandungan serat dan kandungan fines sejalan dengan pengamatan menggunakan SEM. Berdasarkan hasil uji TCLP dan LD50, diketahui bahwa sludge mengandung logam berat, inorganik dan organik yang bersifat stabil, sehingga aman terhadap lingkungan dan dapat dimanfaatkan. Dengan demikian sludge mempunyai potensi sebagai bahan baku pada industri karton chipboard. Kata kunci : Chipboard, industri kertas, LD50, sludge IPAL, TCLP

scholarly journals SAKARIFIKASI LUMPUR PRIMER INDUSTRI KERTAS SECARA FED BATCH MENJADI GLUKOSA UNTUK PEMBUATAN BIOETANOL (FED BATCH SACCHARIFICATION OF PRIMARY PAPER MILL SLUDGE TO PRODUCE FERMENTABLE SUGAR)

2017 ◽  
Vol 7 (02) ◽  
pp. 69
Author(s):  
Mukharomah Nur Aini ◽  
Rina S. Soetopo ◽  
Krisna Septiningrum ◽  
Saepulloh Saepulloh ◽  
Prima Besty Asthary
Keyword(s):  
Paper Mill ◽  
Reducing Sugar ◽  
Raw Material ◽  
Waste Water Treatment Plant ◽  
Fed Batch ◽  
Paper Mill Sludge ◽  
Primary Sludge ◽  
Tissue Paper ◽  
Optimum Dosage ◽  
Virgin Pulp

Research of fed batch saccharification using paper mill primary sludge from Waste Water Treatment Plant (WWTP) has been conducted. The research was conducted in several stages i.e. sludge characterization, determining optimum dosage of cellulase and glucosidase through batch saccharification, and continued with fed batch saccharification. The characterization was performed on 3 primary sludge type from 3 paper mill, which are print paper mill with virgin pulp raw material, paper tissue mill with virgin pulp raw material and core board mill with waste paper raw material. The characterization results showed that the sludge derived from the tissue paper mill with virgin pulp raw material has the highest content of cellulose which is 47.06%, this sludge was used as further research material. The initial trial of batch saccharification at 6% w/w and temperature of 60oC gave optimum dosage of cellulase 9 FPU/g cellulose and β-glucosidase 80 IU/g cellulose with reducing sugar 20.01 g/L and a saccharification efficiency of 63.84%. Fed batch saccharification was performed on tissue paper mill sludge at cellulase and glucosidase enzyme doses respectively 9 FPU/g cellulose and 80 IU/g, temperature 60°C and initial pH of 4 with two variables, which are cumulative solid content (10%, 14%, and 18% w/v) and feed enzyme system (at the beginning and in step). The best results were obtained at fed batch sakarifikasi with gradual enzyme feeding at 14% solids content resulting in reducing sugar 43.899 g/L and efficiency 60.03%. 

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