Effects of Punica Granatum Seed Hydromethanol Extract on Contractility of Isolated Aorta in Female Albino Rats

Antidiabetic action of ethanolic extracts of seed and rind of Punica granatum L. was evaluated on alloxan-induced diabetic albino rats. Both the extracts, each at a dose of 200mg/kg/d, were administered orally for two weeks to alloxan-induced diabetic rats. Blood glucose was estimated every week for two consecutive weeks. For evaluation of probable mechanism of action of the extracts, glycogen estimation was carried out in liver, heart and skeletal muscle and effect on adrenaline-induced hyperglycemia was observed. Both the extracts significantly (p<0.05) reduced the rise in blood glucose induced by alloxan, with the rind extract exhibiting significantly (p<0.05) better activity than seed extract. Both the extracts also produced significant (p<0.05) increase in liver glycogen and significantly (p<0.05) reduced adrenaline-induced hyperglycemia. These results support strong antidiabetic action in favor of P. granatum seed and rind extractsKey words: Punica granatum L.; Ethanolic extracts; Antidiabetic; Alloxan. DOI: 10.3329/sjps.v2i1.5810Stamford Journal of Pharmaceutical Sciences Vol.2(1) 2009: 14-21

Download Full-text

Chemopreventive Efficacy of Punica granatum and Silybum marianum Extracts on Chemically-induced Hepatocellular Carcinoma in Rats

Asian Journal of Applied Sciences ◽

10.24203/ajas.v7i2.5745 ◽

2019 ◽

Vol 7 (2) ◽

Author(s):

Hend Maarof Tag ◽

Ahlem Bargougui ◽

Sara Gamal Alshayyal ◽

Amany Kamal ◽

Hekmat M. Tantawy ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Hepg2 Cells ◽

Punica Granatum ◽

In Vivo Model ◽

Control Group ◽

Albino Rats ◽

Silybum Marianum ◽

Chemically Induced

Punica granatum (POM) and Silybum marianum (MT) receiving attention as potential potent anti-oxidant and anti-mutant agents. In this context, the present study was designed to highlight their effects either in vitro as well as in vivo model of induced Hepatocellular carcinoma (HCC). Human hepatoma (HepG2 cells) were treated with MT and POM to explore their antitumor activity then in vivo were carried out on thirty-six male albino rats divided into six groups (n=6). Two weeks after induction of HCC, rats were co-treated with either MT or POM ethanolic extract (500 mg/kg, orally) daily for 8 weeks. The results displayed marked reduction in the viability of HepG2 cells with IC50 equal to 48.4 and 8.6 Î¼g/mL of POM and MT treatment respectively. Considering, in vivo experiment HCC group displayed significant elevation liver function indices (p<0.05). It also elicited depletion of liver reduced glutathione (GSH), and increased content of liver malondialdehyde (MDA) compared to control group. HCC was proved after a significantly elevated alpha-fetoprotein (AFP) level (p<0.05). All of these measurements were diminished significantly after POM and MT treatments, except the GSH level that was increased significantly. Supplementation of pomegranate and milk thistle extracts had a protective effect against chemically induced HCC.Â

Download Full-text

Evaluation of the Effect of Pomegranate Seed Oil on Healing in a Rat Wound Model With Antioxidant, Vascular, and Histopathological Parameters

The International Journal of Lower Extremity Wounds ◽

10.1177/15347346211040593 ◽

2021 ◽

pp. 153473462110405

Author(s):

A. Nilhan Atsü, MD ◽

Zeynep Tosuner, MD ◽

Tayfun Bilgiç, MD

Keyword(s):

Seed Oil ◽

Blood Perfusion ◽

Punica Granatum ◽

Tissue Level ◽

Punch Biopsy ◽

Enzyme Linked Immunosorbent Assay ◽

Albino Rats ◽

Pomegranate Seed Oil ◽

Significant Difference ◽

Pomegranate Seed

The study aimed to evaluate the efficiency of pomegranate ( Punica granatum) seed oil in wound healing in excised rats. Sixteen female young Wistar Albino Rats weighing approximately 300 to 320 g were randomly divided into 2 groups as the treatment (=pomegranate seed oil group) and control groups in this experiment. Six different wounds 1 cm apart from the midline and each other were formed with a 6 mm punch biopsy instrument. Three wounds were left open (open wound group) whereas 3 wounds were sutured with 4/0 vicryl (closed wound group). Punica granatum seed oil treatment was administered topically to the treatment group, both to open and closed wounds, once a day for 14 days. Parameters for healing were evaluated. Histopathologic examination was performed for the investigation of inflammation, neovascularization, granulation, and fibroblast generation in addition to serologic (enzyme-linked immunosorbent assay) evaluation of rat malondialchehyche, rat glutathione peroxidase, and rat superoxide dismutase. PeriScan PIM 3 System Laser Doppler Blood Perfusion Imager was used for the calculation of blood perfusion. There was a statistically significant difference between inflammation and neovascularization levels and group type on the 14th day in open wounds( P < .05). On the 21st day, the granulation tissue level in the closed wound group was found to be higher in the pomegranate group ( P = 0.000).The results showed that PSE oil is partially effective, although it is not effective in every parameter examined, in the treatment of excised wounds in rats and may be suitable for clinical treatment in humans but large controlled studies are needed.

Download Full-text