Evaluation of the Effect of Pomegranate Seed Oil on Healing in a Rat Wound Model With Antioxidant, Vascular, and Histopathological Parameters

2021 ◽  
pp. 153473462110405
Author(s):  
A. Nilhan Atsü, MD ◽  
Zeynep Tosuner, MD ◽  
Tayfun Bilgiç, MD
Keyword(s):  
Seed Oil ◽  
Blood Perfusion ◽  
Punica Granatum ◽  
Tissue Level ◽  
Punch Biopsy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Albino Rats ◽  
Pomegranate Seed Oil ◽  
Significant Difference ◽  
Pomegranate Seed

The study aimed to evaluate the efficiency of pomegranate ( Punica granatum) seed oil in wound healing in excised rats. Sixteen female young Wistar Albino Rats weighing approximately 300 to 320 g were randomly divided into 2 groups as the treatment (=pomegranate seed oil group) and control groups in this experiment. Six different wounds 1 cm apart from the midline and each other were formed with a 6 mm punch biopsy instrument. Three wounds were left open (open wound group) whereas 3 wounds were sutured with 4/0 vicryl (closed wound group). Punica granatum seed oil treatment was administered topically to the treatment group, both to open and closed wounds, once a day for 14 days. Parameters for healing were evaluated. Histopathologic examination was performed for the investigation of inflammation, neovascularization, granulation, and fibroblast generation in addition to serologic (enzyme-linked immunosorbent assay) evaluation of rat malondialchehyche, rat glutathione peroxidase, and rat superoxide dismutase. PeriScan PIM 3 System Laser Doppler Blood Perfusion Imager was used for the calculation of blood perfusion. There was a statistically significant difference between inflammation and neovascularization levels and group type on the 14th day in open wounds( P < .05). On the 21st day, the granulation tissue level in the closed wound group was found to be higher in the pomegranate group ( P = 0.000).The results showed that PSE oil is partially effective, although it is not effective in every parameter examined, in the treatment of excised wounds in rats and may be suitable for clinical treatment in humans but large controlled studies are needed.

scholarly journals Characterization of constituents, quality and stability of pomegranate seed oil (Punica granatum L.)

2016 ◽  
Vol 36 (1) ◽  
pp. 132-139 ◽  
Author(s):  
Illana Louise Pereira de MELO ◽  
Eliane Bonifácio Teixeira de CARVALHO ◽  
Ana Mara de Oliveira e SILVA ◽  
Luciana Tedesco YOSHIME ◽  
José Augusto Gasparotto SATTLER ◽  
...  
Keyword(s):  
Seed Oil ◽  
Punica Granatum ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed ◽  
Punica Granatum L

scholarly journals Fatty Acids Composition of Red and Purple Pomegranate (Punica granatum L) Seed Oil

2010 ◽  
Vol 1 (2) ◽  
pp. 74 ◽  
Author(s):  
Hartati Soetjipto ◽  
Murda Pradipta ◽  
KH Timotius
Keyword(s):  
Fatty Acids ◽  
Neutral Lipid ◽  
Acid Content ◽  
Seed Oil ◽  
Lipid Fraction ◽  
Punica Granatum ◽  
Punicic Acid ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed ◽  
Punica Granatum L

The aim of this investigation was to determine the content and composition of fatty acid in seed oil of red and purple pomegranate (Punica granatum L). The extraction process was performed by Soxhlet extractor with petroleum ether as solvent. The separation and identification of pomegranate seed oil was done by using GCMS. The total oil content of red and purple  pomegranate  were 128 g/kg d.w  and 103 g/kg d.w respectively. Both showed the same major fatty acids  as palmitic, stearic, oleic, linoleic and  punisic acid. Oleic acid (19-21%) and linoleic acid (20-21%) were found as the most  dominant fatty acids in red pomegranate, whereas purple pomegranate seed oil was dominated by oleic acid (41-43%) and punicic acid  (0-25%). Neutral lipid  fraction of  red and purple pomegranate seed oils was more dominant than glycolipid and phospholipid. Neutral  lipid fraction  of red and purple pomegranate seed oil were  89 % and 91% respectively.  Glycolipid fraction  of red and purple pomegranate seed oil were 8 % and 5 %, whereas phosholipid fraction of red and purple pomegranate seed oil were 3 % and 4 %. The punicic acid content of  total lipid of  purple pomegranate seed oil (PPSO) (0-25%) was higher than red pomegranate (RPSO) (9-16%). On the contrary neutral lipid of red pomegranate showed higher punicic acid content (54-75%) than the purple pomegranate (14-55%). Glycolipid of red pomegranate contained  punicic acid  (0-42%). The punicic acid content  of the phospholipid fraction of  red pomegranate was higher (0-22 %) than the one of purple pomegranate (0-2%).Key words : fatty acid, pomegranate, Punica granatum, punicic acid, seed oil

Pomegranate Seed Oil Modulates Functions and Survival of BV-2 Microglial Cells in vitro

2014 ◽  
Vol 84 (5-6) ◽  
pp. 295-309 ◽  
Author(s):  
Lucia Račková ◽  
Volkan Ergin ◽  
Elif Burcu Bali ◽  
Marcela Kuniaková ◽  
Çimen Karasu
Keyword(s):  
Seed Oil ◽  
Therapeutic Potential ◽  
Fold Increase ◽  
Punica Granatum ◽  
Current Evidence ◽  
No Production ◽  
Omega 3 ◽  
Pomegranate Seed Oil ◽  
Pomegranate Seed

Abstract. Current evidence has demonstrated the immunomodulatory efficacy of omega-3 polyunsaturated fatty acids (PUFAs) in glial cells, suggesting their therapeutic potential for diseases in the central nervous system (CNS). However, conjugated omega-5 PUFAs have also attracted considerable attention because of their suggested anti-inflammatory effects. In the present study, the effect of pomegranate (Punica granatum L.) seed oil (PSEO) (a rich source of omega-5 PUFAs) on the activation of cultured BV-2 microglia was investigated within a 24-hour incubation period. PSEO (25 μg/ml) showed only a slightly smaller inhibitory effect on LPS-stimulated NO production (243 ± 12.5 % of control, p<0.001 vs. 437 ± 9.2 % in stimulated cells) and TNF-α release (87.1 ± 5.62 pg/ml vs. 229 ± 24.4 pg/ml in stimulated cells), as well as iNOS expression (7.36-fold of control, p < 0.01, vs. 17.5-fold increase in stimulated cells) compared to a standardized omega-3 PUFAs mixture (25 μg/ml) and the flavonoid quercetin (25 μmol/l). Unlike quercetin and stobadine, only the PUFA preparations effectively prevented apoptosis of microglia (as confirmed by the suppression of caspase 3 activation) exposed to the toxic concentration of LPS. The PUFA preparations did not provide a notable suppression of the intracellular oxidant generation and did not influence the intracellular distribution of cholesterol (as confirmed by filipin staining). However, they appeared to affect the morphology of activated cells. In conclusion, our data point to the first evidence of immunomodulation and cytoprotection of BV-2 microglia by the pomegranate seed oil, indicating that it may be (comparably to omega-3 PUFAs) efficient against microglia-mediated neuroinflammation while preventing the premature depletion of these immune effector cells in the brain.

scholarly journals A Review Study on Punica granatum L

2015 ◽  
Vol 21 (3) ◽  
pp. 221-227 ◽  
Author(s):  
Erfaneh Shaygannia ◽  
Mahmoud Bahmani ◽  
Behnam Zamanzad ◽  
Mahmoud Rafieian-Kopaei
Keyword(s):  
Antioxidant Activity ◽  
Seed Oil ◽  
Punica Granatum ◽  
Antibacterial Activities ◽  
Inhibitory Effect ◽  
Breast Cancers ◽  
Pomegranate Seed Oil ◽  
Medicinal Properties ◽  
Pomegranate Seed ◽  
Punica Granatum L

Punica granatum L (pomegranate) is a deciduous shrub, native to Iran. Nowadays, besides its use as a fruit, its medicinal properties have attracted the interest of researchers of many countries. Pomegranate fruit has medicinal properties such as anti-inflammatory and antibacterial activities. The pomegranate seed oil has inhibitory effect on skin and breast cancers. The pomegranate seed oil has phytoestrogenic compounds and the fruit is rich in phenolic compounds with strong antioxidant activity. Ellagic acid is one of the main components of pomegranate with phenolic structure and antioxidant activity. This review article presents the recently published findings on different aspects of this plant focusing on its medicinal properties

In Vitro Estimation of Photo-Protective Potential of Pomegranate Seed Oil and Development of a Nanoformulation

2019 ◽  
Vol 15 (1) ◽  
pp. 87-102 ◽  
Author(s):  
Surbhi Dhawan ◽  
Sanju Nanda
Keyword(s):  
Particle Size ◽  
Zeta Potential ◽  
Seed Oil ◽  
Topical Delivery ◽  
Inflammatory Activity ◽  
Pomegranate Seed Oil ◽  
Protective Potential ◽  
Release Studies ◽  
Pomegranate Seed

Background: Since ancient times, people have been using natural resources for photoprotection purposes. One such highly recognised natural agent is pomegranate seed oil, considered as wonder oil owing to the presence of several beneficial phytoconstituents. </P><P> Objective: The study aimed to establish the photoprotective potential of pomegranate seed oil through various in vitro and biochemical studies along with the formation of nanoemulsion, an efficient topical delivery system for the oil. </P><P> Method: Photo-protective potential of the oil was estimated by determining in vitro antioxidant and anti-inflammatory activity, total phenolic content, anti elastase, antihyaluronidase and anticollagenase activities of the oil. Ultrasonication method was used to formulate nanoemulsions. The optimisation was done following the central composite design. The characterisation was done by particle size analysis, zeta potential, polydispersity index, pH, viscosity, stability testing and transmission electron microscopy. The optimised nanoemulsion was loaded into a gel base for topical application and further release studies were carried out. </P><P> Results: The IC50 values of anti-elastase, anti-collagenase and anti-hyaluronidase were found to be 309 mg/ml, 4 mg/ml and 95 mg/ml respectively. The results of anti-oxidant and anti-inflammatory activity were also significant, which thereby established the photo-protective potential of the oil. The optimum batch 2 had particle size 83.90 nm, 0.237 PDI and -5.37 mV zeta potential. The morphology was confirmed by TEM. Batch 2 was incorporated into a gel base and release studies showed 74.12 % release within 7 hours. </P><P> Conclusion: Pomegranate seed oil possesses a potential photo-protective ability. Nanoemulsions proved to be a promising carrier for the topical delivery of the oil.

Evaluation of green extraction processes, lipid composition and antioxidant activity of pomegranate seed oil

2021 ◽  
Author(s):  
E. Rojo-Gutiérrez ◽  
O. Carrasco-Molinar ◽  
J. M. Tirado-Gallegos ◽  
A. Levario-Gómez ◽  
M. L. Chávez-González ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Lipid Composition ◽  
Seed Oil ◽  
Green Extraction ◽  
Pomegranate Seed Oil ◽  
Extraction Processes ◽  
Pomegranate Seed
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