The genetic diversity of the Vietnamese H’mong dog breed based on mitochondrial HV1 analysis

The H'mong docked tail is indigenous breed of dogs distributed in mountainous area of northern Vietnam. These dogs possess positive characteristics such as intelligence, agility, good health, good shape, human friendliness, ease of training and fully meet the criteria of a war dog. We had studied the diversity and origin of Vietnamese H’mong dogs based on the molecular data of the mitochondrial HV1 region. A total of seventy individual dogs were sampled from Lao Cai, Ha Giang and Hai Phong provinces of northern Vietnam and were sequenced. Among the targeted dogs, 29 haplotypes were recorded and divided into four main groups: A, B, C and E. In our study, nine new haplotypes were recorded and all of them belong to haplotype group A. Group A was the most common with 24 haplotypes (82.76%) followed by C, E and B with 2 (6.9%), 2 (6.9%) and 1 haplotypes (3.4%) respectively. The genetic variations in the H'mong dogs were high with nucleotide diversity (Pi = 0.03179), haplotype diversity (Hd = 0.95648) and average number of nucleotide differences (Kt = 5.18384) and the p-genetic distances range was from 0.2 to 2.9%. This study has provided a valuable platform for breeding and conservation and management of the species in Vietnam.

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Genetic diversity of H’mong short tail dog based on sequencing of the D-loop hypervariable -1 region (HV1)

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/14858 ◽

2021 ◽

Vol 19 (2) ◽

pp. 245-257

Author(s):

Pham Thanh Hai ◽

Bui Xuan Phuong ◽

Tran Huu Coi ◽

Phung Thanh Tung ◽

Ngo Quang Duc ◽

...

Keyword(s):

Genetic Diversity ◽

Haplotype Diversity ◽

Good Health ◽

Base Substitution ◽

Nucleotide Polymorphisms ◽

Northern Vietnam ◽

Short Tail ◽

Hunting Dogs ◽

Indel Mutation ◽

D Loop

The H'mong short tail dog is breed indigenous dogs, distributed in mountainousareas of northern Vietnam. H'mong short tail dog possesses many valuable properties such as intelligence, agility, good health, good shape, human friendliness, ease of training and it can fully meet the needs of war Dogs intelligence, strength, good parenting, people friendly and more importantly, still keeping wild characteristics of hunting dogs. The total 45 samples (blood) collected from 45 individuals in two provinces of Northern Vietnam (Ha Giang and Lao Cai), were used to assess genetic diversity based on sequencing hypervariable – 1 region (HV1) in D-loop genes. In the current study showed that genetic diversity of H'mong short tail dog was high with nucleotide diversity (Pi = 0.00801), haplotype diversity (Hd = 0.96162) and average number of nucleotide differences (Kt = 5.18384). Furthermore, 25 different haplotypes were recorded and divided into four main groups: A, B, C, and E. Of which, seven new haplotypes in haplogroups A (An1 to An7) and 18 haplotypes have been published in the world. In addition, H'mong short tail dog was found rare haplogroups (B1, C2, E1 and E4). Notably, there is none individuals contain haplotype of haplogroups (D and F). H'mong short tail dog were identified 38 single nucleotide polymorphisms, including 32 nucleotide base substitution/base insertion and 6 nucleotide indel mutation. Almost mutation was transversion (31/32) and only one nucleotide transition mutations. Phylogenetic tree shown that H'mong short tail dog have close relationship with dogs origin from East Asia (China, Japan and Korea).

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A new riparian species of Ramalina (Ramalinaceae) from Brazil, with a key to neotropical saxicolous species

The Lichenologist ◽

10.1017/s0024282918000361 ◽

2018 ◽

Vol 50 (5) ◽

pp. 541-553 ◽

Cited By ~ 2

Author(s):

Emerson Luiz GUMBOSKI ◽

Sionara ELIASARO ◽

Mayara Camila SCUR ◽

Aline Pedroso LORENZ-LEMKE ◽

Rosa Mara BORGES DA SILVEIRA

Keyword(s):

New Species ◽

Ribosomal Dna ◽

Habitat Preference ◽

Molecular Data ◽

Genetic Distances ◽

Integrative Approach ◽

Chemical Variation ◽

Southern Brazil ◽

Riparian Species ◽

High Altitude Grasslands

AbstractThe new species Ramalina fleigiae from Brazil is described growing on rocks in riverbeds in high altitude grasslands of southern Brazil. It grows in areas with constant water flow, sometimes almost immersed, and always in exposed habitats. Through an integrative approach, the detailed description of R. fleigiae includes morphological, anatomical, ecological, chemical and molecular data. Ribosomal DNA-based phylogenies suggest that R. fleigiae is more closely related to a species that shares its habitat preference (R. laevigata) than to the morphologically and chemically similar R. exiguella and R. gracilis. Ramalina fleigiae and R. laevigata can be distinguished by thallus morphology (irregularly flat branches in R. fleigiae vs. flat to canaliculate in R. laevigata) and pattern of chondroid tissue, as genetic distances between them are compatible with the interspecific range. It is possible that many species of Ramalina still remain hidden within the morphological or chemical variation of currently accepted species. Combining ecological, anatomical and molecular data will improve our future understanding of this genus.

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Morphological description and DNA barcoding of Chaetocladius (Chaetocladius) elisabethae sp. nov. (Diptera: Chironomidae: Orthocladiinae) from the Moscow Region

Zootaxa ◽

10.11646/zootaxa.4403.2.9 ◽

2018 ◽

Vol 4403 (2) ◽

pp. 378 ◽

Cited By ~ 1

Author(s):

EUGENYI A. MAKARCHENKO ◽

MARINA A. MAKARCHENKO ◽

ALEXANDER A. SEMENCHENKO ◽

DMITRY M. PALATOV

Keyword(s):

Dna Barcoding ◽

Mitochondrial Gene ◽

Interspecific Variation ◽

Instar Larva ◽

Molecular Data ◽

Genetic Distances ◽

Moscow Region ◽

Morphological Description ◽

Closely Related Species ◽

Male Pupa

Illustrated descriptions of the adult male, pupa and fourth instar larva, as well as DNA barcoding results of Chaetocladius (Chaetocladius) elisabethae sp. nov. in comparison with closely related species of Chaetocladius s. str. from the Moscow Region are provided. A reference 658 bp barcode sequence from a fragment of the mitochondrial gene cytochrome oxidase I (COI) was used as a tool for species delimitation. Comparisons with corresponding regions of COI between C. (s. str.) elisabethae sp. nov. and other species of the subgenus produced K2P genetic distances of 0.11–0.16, values well associated with interspecific variation. The barcodes of the new species were identical to the Chaetocladius sp. 2ES in BOLD systems. Molecular data were also used for the reconstruction of the phylogenetic relationships within the subgenus Chaetocladius s. str.

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Debating phylogenetic relationships of the scleractinian Psammocora: molecular and morphological evidences

Contributions to Zoology ◽

10.1163/18759866-07601004 ◽

2007 ◽

Vol 76 (1) ◽

pp. 35-54 ◽

Cited By ~ 66

Author(s):

Francesca Benzoni ◽

Fabrizio Stefani ◽

Jaroslaw Stolarski ◽

Michel Pichon ◽

Guillaume Mitta ◽

...

Keyword(s):

Molecular Phylogeny ◽

Phylogenetic Relationships ◽

Molecular Data ◽

Genetic Distances ◽

The Other ◽

Congeneric Species ◽

Genetic Affinity ◽

Phylogenetic Structure ◽

Skeletal Structures ◽

The Family

The phylogenetic relationships of the scleractinian genus Psammocora with the other genera traditionally included in the family Siderastreidae and some Fungiidae are assessed based on combined skeletal and molecular data. P. explanulata differs from the other examined congeneric species (P. contigua, P. digitata, P. nierstraszi , P. profundacella, P. superficialis, and P. stellata) in possessing interstomatous septa between adult corallites, costae, and in having continuous buttress-like structures joining septal faces (i.e., fulturae) which typically occur in fungiids. These characters are shared with Coscinaraea wellsi but not with the remainder of the examined siderastreids (the congeneric C. columna, and Anomastraea irregularis, Horastrea indica, Pseudosiderastrea tayamai, Siderastrea savignyana) whose septa are interconnected by typical synapticulae. Most of the examined species form septa with distinct transverse groups of centers of calcification, a biomineralization pattern typical of the Robusta clade. The observations on skeletal structures corroborate the results of the ITS2 and 5.8S molecular phylogeny. C. wellsi and P. explanulata are phylogenetically very close to each other and show closer genetic affinity with the examined Fungiidae (Halomitra pileus, Herpolitha limax, Fungia paumotensis, and Podabacia crustacea) than with the other species in the genera Psammocora and Coscinaraea, or with any other siderastreid. Our results show that neither Psammocora nor Coscinaraea are monophyletic genera. The high genetic distances between the species of Siderastreidae, especially between Pseudosiderastrea tayamai and Siderastrea savignyana on one side and the other genera on the other, suggest a deep divergence in the phylogenetic structure of the family.

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Caridina biyiga sp. nov., a new freshwater shrimp (Crustacea: Decapoda: Atyidae) from Leichhardt Springs, Kakadu National Park, Australia, based on morphological and molecular data, with a preliminary illustrated key to Northern Territory Caridina

Zootaxa ◽

10.11646/zootaxa.4695.1.1 ◽

2019 ◽

Vol 4695 (1) ◽

pp. 1-25

Author(s):

JOHN W. SHORT ◽

TIMOTHY J. PAGE ◽

CHRISTOPHER L. HUMPHREY

Keyword(s):

New Species ◽

16S Rdna ◽

National Park ◽

Strong Support ◽

Molecular Data ◽

Genetic Distances ◽

Northern Territory ◽

Coi Gene ◽

Gene Fragment ◽

Morphological And Molecular Data

Caridina biyiga sp. nov. is described from Leichhardt Springs, Kakadu National Park, Northern Territory, Australia, based on morphological and molecular data. The new species is highly distinctive among its congeners in having the dactylus of pereiopod 5 similar to the dactyli of pereiopods 3–4 and lacking the comb-like row of spiniform setae typical of the genus. The branchial formula is also distinctive for the genus in lacking an arthrobranch at the base of pereiopod 1 and in having a vestigial epipod on maxilliped 1. Despite the distinctive morphology of the new species, the molecular data did not provide strong support for the recognition of a new genus. Phylogenetic analyses of Australian Caridina using the mitochondrial 16S rDNA gene fragment placed the new species in a well-supported clade containing at least one typical species of Caridina. Within this clade, referred to as the ‘thermophila’ group, Kimura 2-parameter (K2P) genetic distances of 16S rDNA between the new species and sister taxa ranged from 5.1–6.0%. Analyses using the mitochondrial 3’ COI gene fragment from species of the ‘thermophila’ group yielded K2P genetic distances between the new species and its sister taxa ranging from 10.4–15.1%. A preliminary illustrated key to Northern Territory Caridina is provided. The conservation significance of Leichhardt Springs and its aquatic fauna are also briefly discussed.

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A new record of the genus Yua (Vitaceae) from Vietnam

Phytotaxa ◽

10.11646/phytotaxa.255.3.9 ◽

2016 ◽

Vol 255 (3) ◽

pp. 274 ◽

Cited By ~ 1

Author(s):

VIET CUONG DANG ◽

LIMIN LU ◽

VAN HIEU NGUYEN ◽

SADAF HABIB ◽

RUSSELL L. BARRETT ◽

...

Keyword(s):

New Record ◽

Molecular Data ◽

Northern Vietnam ◽

Northern India ◽

Diagnostic Characteristics ◽

Lao Cai ◽

Morphological And Molecular Data

Yua C.L. Li (Vitaceae), a genus previously known from China, Nepal and India, is newly recorded from Vietnam. A specimen discovered in the rainforest of Lao Cai province, northern Vietnam was identified as Yua austro-orientalis (F.P. Metcalf) C.L. Li based on both morphological and molecular data. To improve our understanding of relationships between Yua and Parthenocissus Planch., we present diagnostic characteristics for these two genera. We discuss leaf, stem and seed features used to distinguish between Y. austro-orientalis and Y. thomsonii (M.A. Lawson) C.L. Li, a species widely distributed in China, Nepal and northern India. The opportunity is taken to review the typification of all Yua taxa. A lectotype is selected for Vitis thomsonii M.A. Lawson.

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Taxonomic Revision of Cuphea sect. Euandra subsect. Oidemation (Lythraceae)

Phytotaxa ◽

10.11646/phytotaxa.113.1.1 ◽

2013 ◽

Vol 113 (1) ◽

pp. 1 ◽

Cited By ~ 2

Author(s):

SHIRLEY A. GRAHAM ◽

TACIANA B. CAVALCANTI

Keyword(s):

Chromosome Numbers ◽

Taxonomic Revision ◽

Molecular Data ◽

Seed Morphology ◽

New Combination ◽

Phylogenetic Information ◽

Eastern Brazil ◽

Pollen Types ◽

Group A ◽

Eleven Species

Cuphea sect. Euandra subsect. Oidemation comprises a non-monophyletic group of species defined by possession of a fire-resistant xylopodium. The xylopodium is a frequent feature of plants occurring in the seasonally and ecologically dry cerrados and savannas of South America. Subsect. Oidemation has diversified particularly in these habitats in eastern Brazil and adjacent Paraguay. Twenty species and five varieties are recognised: eleven species are endemic to Brazil; one each is endemic to Paraguay and Florida, USA; and seven are distributed in Brazil and adjacent areas of Bolivia, Paraguay, Uruguay and/or Argentina. Their morphology, including pollen and seed morphology, and chromosome numbers are reviewed. The presence of at least three major pollen types and two base chromosome numbers suggests the subsection is para- or polyphyletic; phylogenetic information based on molecular data for species of the subsection is limited. Diverse diploid, tetraploid, and hexaploid chromosome numbers point to polyploidy as an important mechanism of speciation in this group. A key to the species is accompanied by descriptions, illustrations and distribution maps.The subsection is lectotypified by C. retrorsicapilla and lectotypes are designated for C. acicularis, C. aspera, C. confertiflora, C. crulsiana, C. emarginata, C. enneanthera, C. excoriata, C. ferruginea, C. ferruginea var. acuminata, C. hyssopoides, C. remotifolia, C. retrorsicapilla, C. sperguloides, C. spermacoce var. angustata, and C. spermacoce var. elongata. A new combination is made for C. retrorsicapilla var. enneanthera.

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A new species of cat-eyed snake (Serpentes: Dipsadinae: Leptodeirini) from the Andes of southern Ecuador

Zootaxa ◽

10.11646/zootaxa.4895.3.3 ◽

2020 ◽

Vol 4895 (3) ◽

pp. 357-380

Author(s):

OMAR TORRES-CARVAJAL ◽

JUAN C. SÁNCHEZ-NIVICELA ◽

VALENTINA POSSE ◽

ELVIS CELI ◽

CLAUDIA KOCH

Keyword(s):

New Species ◽

Nuclear Dna ◽

Sequence Data ◽

Molecular Data ◽

Genetic Distances ◽

Dna Sequence Data ◽

The Andes ◽

Southern Ecuador ◽

Morphological And Molecular Data ◽

A New Species

Leptodeira is one of the most widespread and taxonomically problematic snake taxa in the Americas. Here we describe a new species of Leptodeira from the Andes of southern Ecuador based on morphological and molecular data. The new species is geographically close and morphologically similar to L. ornata and L. larcorum, from which it can be distinguished by having smaller dorsal body blotches, a longer tail, and shorter spines on the hemipenial body. The shortest genetic distances between the new species and its congeners are 0.02 (16S), 0.05 (cytb), and 0.18 (ND4). The new species is restricted to the Jubones River Basin in southern Ecuador, an area of endemism for other reptile species. Our phylogenetic analysis based on mitochondrial and nuclear DNA sequence data also supports recognition of the names L. larcorum (restricted to Peru) for “L. septentrionalis larcorum”, and L. ornata for populations of “L. s. ornata” from central and eastern Panama, western Colombia, and western Ecuador. However, some samples of “L. s. ornata” from Panama and Costa Rica, as well as the new species described herein, are not included within or more closely related to L. ornata, which is sister to the clade (L. bakeri, L. ashmeadii).

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Morphological and molecular data to determine the origin and taxonomic status of Prosopis chilensis var. riojana (Fabaceae, Mimosoideae)

Canadian Journal of Botany ◽

10.1139/b03-078 ◽

2003 ◽

Vol 81 (9) ◽

pp. 905-917 ◽

Cited By ~ 7

Author(s):

Soledad Vázquez-Garcidueñas ◽

Ramón A Palacios ◽

Jenny Segovia-Quiroz ◽

Juan T Frías-Hernández ◽

Victor Olalde-Portugal ◽

...

Keyword(s):

Random Amplified Polymorphic Dna ◽

Taxonomic Status ◽

Molecular Data ◽

Genetic Distances ◽

Group Method ◽

Sympatric Populations ◽

Arithmetic Means ◽

Genetic Characteristics ◽

Genetic Traits ◽

Prosopis Chilensis

This study analyzes the morphological and genetic characteristics of three sympatric populations of Prosopis from Argentina. Although morphological and geographical data suggest that Prosopis chilensis var. riojana is an interspecific hybrid of Prosopis chilensis var. chilensis and Prosopis flexuosa var. flexuosa, no correlation was found between morphological traits and genetic distances generated by random amplified polymorphic DNA - polymerase chain reaction (RAPD-PCR). Genetic similarity is greater among P. chilensis var. chilensis and P. flexuosa var. flexuosa than between either of these two taxa with P. chilensis var. riojana. Also, P. chilensis var. riojana has unique genetic markers that are absent from its putative parents. Additionally, dendrograms generated by unweighted pair group method with arithmetic means (UPGMA) and Neighbor-Joining clustering criteria group P. chilensis var. chilensis and P. flexuosa var. flexuosa apart from P. chilensis var. riojana. Possibilities for the lack of congruence between morphology and RAPD markers are discussed. The results obtained are insufficient to conclusively establish the origin of P. chilensis var. riojana; however, the morphological and genetic traits observed suggest this taxon is well differentiated from P. chilensis var. chilensis and P. flexuosa var. flexuosa.Key words: genetic distances, hybridization, morphology, Prosopis, RAPD, rare taxon.

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