scholarly journals Surveying the Delivery Methods of CRISPR/Cas9 for ex vivo Mammalian Cell Engineering

2016 ◽  
Vol 70 (6) ◽  
pp. 439-442 ◽  
Author(s):  
William J. Kelton ◽  
Theresa Pesch ◽  
Stefan Matile ◽  
Sai T. Reddy
Keyword(s):  
Mammalian Cell ◽  
Ex Vivo ◽  
Cell Engineering ◽  
Delivery Methods

scholarly journals Bacterial encapsulins as orthogonal compartments for mammalian cell engineering

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Felix Sigmund ◽  
Christoph Massner ◽  
Philipp Erdmann ◽  
Anja Stelzl ◽  
Hannes Rolbieski ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Cell Engineering

CRISPR Toolbox for Mammalian Cell Engineering

2019 ◽  
pp. 185-206
Author(s):  
Daria Sergeeva ◽  
Karen Julie la Cour Karottki ◽  
Jae Seong Lee ◽  
Helene Faustrup Kildegaard
Keyword(s):  
Mammalian Cell ◽  
Cell Engineering

scholarly journals Allogenic Fc Domain-Facilitated Uptake of IgG in Nasal Lamina Propria: Friend or Foe for Intranasal CNS Delivery?

Pharmaceutics ◽  
2018 ◽  
Vol 10 (3) ◽  
pp. 107 ◽  
Author(s):  
Simone Ladel ◽  
Johannes Flamm ◽  
Arghavan Soleimani Zadeh ◽  
Dorothea Filzwieser ◽  
Julia-Christina Walter ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Lamina Propria ◽  
Messenger Rna ◽  
Ex Vivo ◽  
Neurological Diseases ◽  
Olfactory Mucosa ◽  
Basal Cells ◽  
Delivery Methods ◽  
Lymphoid Follicles ◽  
Brain Drug Delivery

Background: The use of therapeutic antibodies for the treatment of neurological diseases is of increasing interest. Nose-to-brain drug delivery is one strategy to bypass the blood brain barrier. The neonatal Fc receptor (FcRn) plays an important role in transepithelial transcytosis of immunoglobulin G (IgG). Recently, the presence of the FcRn was observed in nasal respiratory mucosa. The aim of the present study was to determine the presence of functional FcRn in olfactory mucosa and to evaluate its role in drug delivery. Methods: Immunoreactivity and messenger RNA (mRNA) expression of FcRn was determined in ex vivo porcine olfactory mucosa. Uptake of IgG was performed in a side-by-side cell and analysed by immunofluorescence. Results: FcRn was found in epithelial and basal cells of the olfactory epithelium as well as in glands, cavernous bodies and blood vessels. Allogenic porcine IgGs were found time-dependently in the lamina propria and along axonal bundles, while only small amounts of xenogenic human IgGs were detected. Interestingly, lymphoid follicles were spared from allogenic IgGs. Conclusion: Fc-mediated transport of IgG across the nasal epithelial barrier may have significant potential for intranasal delivery, but the relevance of immune interaction in lymphoid follicles must be clarified to avoid immunogenicity.

Systematic Evaluation of Site-Specific Recombinant Gene Expression for Programmable Mammalian Cell Engineering

2019 ◽  
Vol 8 (4) ◽  
pp. 758-774 ◽  
Author(s):  
Nuša Pristovšek ◽  
Saranya Nallapareddy ◽  
Lise Marie Grav ◽  
Hooman Hefzi ◽  
Nathan E. Lewis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Mammalian Cell ◽  
Cell Engineering ◽  
Systematic Evaluation ◽  
Recombinant Gene Expression ◽  
Site Specific ◽  
Recombinant Gene

Stem cell engineering towards the optimization of the ex-vivo expansion of human hematopoietic stem/progenitor cells for cellular therapies

Cytotherapy ◽  
2014 ◽  
Vol 16 (4) ◽  
pp. S60
Author(s):  
C.L. da Silva ◽  
P.Z. Andrade ◽  
A.M. Soure ◽  
F. Dos Santos ◽  
G. Almeida-Porada ◽  
...  
Keyword(s):  
Stem Cell ◽  
Progenitor Cells ◽  
Ex Vivo ◽  
Ex Vivo Expansion ◽  
Cell Engineering ◽  
Hematopoietic Stem ◽  
Cellular Therapies ◽  
Stem Cell Engineering

scholarly journals Enhancement of Curcumin Anti-Inflammatory Effect via Formulation into Myrrh Oil-Based Nanoemulgel

Polymers ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 577 ◽  
Author(s):  
Wafaa E. Soliman ◽  
Tamer M. Shehata ◽  
Maged E. Mohamed ◽  
Nancy S. Younis ◽  
Heba S. Elsewedy
Keyword(s):  
Ex Vivo ◽  
Skin Permeation ◽  
In Vitro Release ◽  
Aqueous Solubility ◽  
Delivery Methods ◽  
Anti Cancer ◽  
Permeation Studies ◽  
Anti Inflammatory

Background: Curcumin (Cur) possesses a variety of beneficial pharmacological properties including antioxidant, antimicrobial, anti-cancer and anti-inflammatory activities. Nevertheless, the low aqueous solubility and subsequent poor bioavailability greatly limits its effectiveness. Besides, the role of myrrh oil as an essential oil in treating inflammatory disorders has been recently demonstrated. The objective of the current investigation is to enhance Cur efficacy via developing Cur nanoemulgel, which helps to improve its solubility and permeability, for transdermal delivery. Methods: The formulated preparations (Cur gel, emulgel and nanoemulgel) were evaluated for their physical appearance, spreadability, viscosity, particle size, in vitro release and ex vivo drug permeation studies. The in vivo anti-inflammatory activity was estimated using the carrageenan-induced rat hind paw edema method. Results: The formulated Cur-loaded preparations exhibited good physical characteristics that were in the acceptable range of transdermal preparations. The release of Cur from gel, emulgel and nanoemulgel after 12 h was 72.17 ± 3.76, 51.93 ± 3.81 and 62.0 ± 3.9%, respectively. Skin permeation of Cur was significantly (p < 0.05) improved when formulated into nanoemulgel since it showed the best steady state transdermal flux (SSTF) value (108.6 ± 3.8 µg/cm2·h) with the highest enhancement ratio (ER) (7.1 ± 0.2). In vivo anti-inflammatory studies proved that Cur-loaded nanoemulgel displayed the lowest percent of swelling (26.6% after 12 h). Conclusions: The obtained data confirmed the potential of the nanoemulgel dosage form and established the synergism of myrrh oil and Cur as an advanced anti-inflammatory drug.

scholarly journals A Comparison of Drug Delivery into Skin Using Topical Solutions, Needle Injections and Jet Injections

2019 ◽  
Author(s):  
Katharina Cu ◽  
Ruchi Bansal ◽  
Samir Mitragotri ◽  
David Fernandez Rivas
Keyword(s):  
Rhodamine B ◽  
Continuous Wave ◽  
Ex Vivo ◽  
Delivery Methods ◽  
Injection Method ◽  
Dispersion Velocity ◽  
Direct Red 81 ◽  
Red Dye ◽  
Permanent Make Up ◽  
Needle Injection

AbstractDrug diffusion within the skin with a needle-free micro-jet injection (NFI) device was compared with two well-established delivery methods: topical application and solid needle injection. A permanent make-up (PMU) machine, normally used for dermal pigmentation, was utilized as a solid needle injection method. For NFIs a continuous wave (CW) laser diode was used to create a bubble inside a microfluidic device containing a light absorbing solution. Each method delivered two different solutions intoex-vivoporcine skin. The first solution consisted of a red dye (direct red 81) and rhodamine B in water. The second solution was direct red 81 and rhodamine B in water and glycerol. For PMU experiments, the skin samples were kept stationary and the diffusion depth, width and surface area were measured. The NFI has a higher vertical dispersion velocity of 3 × 105μm/s compared to topical (0.1 μm/s) and needle injection (53μm/s). The limitations and advantages of each method are discussed, and we conclude that the micro-jet injector represents a fast and minimally invasive injection method, while the solid needle injector causes notably tissue damage. In contrast, the topical method had the slowest diffusion rate but causes no visible damage to the skin.

scholarly journals Delivery Strategies for Skin: Comparison of Nanoliter Jets, Needles and Topical Solutions

2019 ◽  
Vol 48 (7) ◽  
pp. 2028-2039 ◽  
Author(s):  
Katharina Cu ◽  
Ruchi Bansal ◽  
Samir Mitragotri ◽  
David Fernandez Rivas
Keyword(s):  
Rhodamine B ◽  
Continuous Wave ◽  
Ex Vivo ◽  
Delivery Methods ◽  
Injection Method ◽  
Dispersion Velocity ◽  
Direct Red 81 ◽  
Red Dye ◽  
Permanent Make Up ◽  
Needle Injection

Abstract Drug diffusion within the skin with a needle-free micro-jet injection (NFI) device was compared with two well-established delivery methods: topical application and solid needle injection. A permanent make-up (PMU) machine, normally used for dermal pigmentation, was utilized as a solid needle injection method. For NFIs a continuous wave (CW) laser diode was used to create a bubble inside a microfluidic device containing a light absorbing solution. Each method delivered two different solutions into ex vivo porcine skin. The first solution consisted of a red dye (direct red 81) and rhodamine B in water. The second solution was direct red 81 and rhodamine B in water and glycerol. We measured the diffusion depth, width and surface area of the solutions in all the injected skin samples. The NFI has a higher vertical dispersion velocity of 3 × 105μm/s compared to topical (0.1 μm/s) and needle injection (53 μm/s). The limitations and advantages of each method are discussed, and we conclude that the micro-jet injector represents a fast and minimally invasive injection method, while the solid needle injector causes notable tissue damage. In contrast, the topical method had the slowest diffusion rate but causes no visible damage to the skin.

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