EFFECT OF ELECTROMAGNETIC FIELDS TREATMENT ON PEROXIDASE ACTIVITY AND POLYPHENOL CONTENT IN CABBAGE DURING STORAGE

Author(s):  
Т.В. ПЕРШАКОВА ◽  
Г.А. КУПИН ◽  
А.А. ТЯГУЩЕВА ◽  
В.Н. АЛЁШИН

Для увеличения масштабов производства и переработки растительного сочного сырья и сокращения использования химических пестицидов применяются различные виды физической обработки, в том числе электромагнитные поля (ЭМП). Представлены результаты изучения влияния ЭМП крайне низкой частоты (ЭМП КНЧ) на активность пероксидазы и содержание полифенольных веществ в белокочанной капусте сорта Белтис и цветной капусте сорта Ардент при хранении. Образцы обрабатывались ЭМП (25 Гц, 10 мТл, 30 мин) на лабораторной экспериментальной установке и хранились в течение 21 сут при t (4±1)°С и относительной влажности воздуха (75±3)%. Установлено, что обработка капусты белокочанной привела к увеличению активности пероксидазы на 70% через 7 сут, а через 21 сут хранения - увеличению пероксидазы на 25% и уменьшению полифенольных веществ на 13,7%. В обработанных образцах цветной капусты увеличилась активность пероксидазы на 54% через 7 сут и на 62 % через 14 сут хранения, а также количество полифенольных веществ на 8% через 21 сут хранения. Изучение закономерностей влияния обработки растительного сочного сырья ЭМП КНЧ на индукцию резистентности позволяет разработать на их основе новые способы хранения. In order to increase the scale of production and processing of vegetable juicy raw materials, it is necessary to timely develop new efficient, economical and environmentally friendly storage technologies. One possible way to reduce the use of chemical pesticides is through the use of various types of physical treatments, including electromagnetic fields. The article presents the results of studying the influence of extremely low frequency electromagnetic fields on the peroxidase activity and the total polyphenolic content in the white cabbage cultivar Beltis and the cauliflower cultivar Ardent during storage. The objects of research were treated with an electromagnetic field (25 Hz, 10mT, 30 min) in a laboratory experimental setup and were stored for 21 days at a temperature of 4±1°С and air relative humidity of (75±3)%. It was found that, in comparison with the control, the treatment of white cabbage leads to an increase in peroxidase activity by 70% after 7 days and by 25% after 21 days of storage, and an increase in peroxidase by 25% and a decrease in polyphenolic substances by 13,7% after 21 days of storage. It was also found that, in comparison with the control, the treatment of cauliflower leads to an increase in peroxidase activity by 54% after 7 days and by 62% after 14 days of storage, and leads to an 8% increase in the content of polyphenolic substances after 21 days of storage. The study of the regularities of the influence of the treatment of vegetable juicy raw materials with electromagnetic fields of extremely low frequencies on the induction of resistance can make it possible to develop new storage methods on their basis.

2019 ◽  
Vol 21 (1) ◽  
pp. 152 ◽  
Author(s):  
Olga García-Minguillán ◽  
Raquel Prous ◽  
Maria del Carmen Ramirez-Castillejo ◽  
Ceferino Maestú

The effects produced by electromagnetic fields (EMFs) on human beings at extremely low frequencies (ELFs) have being investigated in the past years, across in vitro studies, using different cell lines. Nevertheless, the effects produced on cells are not clarified, and the cellular mechanisms and cell-signaling processes involved are still unknown. This situation has resulted in a division among the scientific community about the adequacy of the recommended level of exposure. In this sense, we consider that it is necessary to develop long-term exposure studies and check if the recommended levels of EMFs are under thermal effects. Hence, we exposed CT2A cells to different EMFs at different ELFs at short and long times. Our results showed frequency dependence in CT2A exposed during 24 h to a small EMF of 30 μT equal to those originated by the Earth and frequency dependence after the exposure during seven days to an EMF of 100 µT at different ELFs. Particularly, our results showed a remarkable cell viability decrease of CT2A cells exposed to EMFs of 30 Hz. Nevertheless, after analyzing the thermal effects in terms of HSP90 expression, we did not find thermal damages related to the differences in cell viability, so other crucial cellular mechanism should be involved.


2009 ◽  
Vol 35 (3) ◽  
pp. 207-210
Author(s):  
G. I. Kas’yanov ◽  
A. A. Zaporozhskii ◽  
M. G. Baryshev

2017 ◽  
Vol 284 (1864) ◽  
pp. 20171670 ◽  
Author(s):  
Molly C. Womack ◽  
Jakob Christensen-Dalsgaard ◽  
Luis A. Coloma ◽  
Juan C. Chaparro ◽  
Kim L. Hoke

Sensory losses or reductions are frequently attributed to relaxed selection. However, anuran species have lost tympanic middle ears many times, despite anurans' use of acoustic communication and the benefit of middle ears for hearing airborne sound. Here we determine whether pre-existing alternative sensory pathways enable anurans lacking tympanic middle ears (termed earless anurans) to hear airborne sound as well as eared species or to better sense vibrations in the environment. We used auditory brainstem recordings to compare hearing and vibrational sensitivity among 10 species (six eared, four earless) within the Neotropical true toad family (Bufonidae). We found that species lacking middle ears are less sensitive to high-frequency sounds, however, low-frequency hearing and vibrational sensitivity are equivalent between eared and earless species. Furthermore, extratympanic hearing sensitivity varies among earless species, highlighting potential species differences in extratympanic hearing mechanisms. We argue that ancestral bufonids may have sufficient extratympanic hearing and vibrational sensitivity such that earless lineages tolerated the loss of high frequency hearing sensitivity by adopting species-specific behavioural strategies to detect conspecifics, predators and prey.


Geophysics ◽  
1992 ◽  
Vol 57 (6) ◽  
pp. 854-859 ◽  
Author(s):  
Xiao Ming Tang

A new technique for measuring elastic wave attenuation in the frequency range of 10–150 kHz consists of measuring low‐frequency waveforms using two cylindrical bars of the same material but of different lengths. The attenuation is obtained through two steps. In the first, the waveform measured within the shorter bar is propagated to the length of the longer bar, and the distortion of the waveform due to the dispersion effect of the cylindrical waveguide is compensated. The second step is the inversion for the attenuation or Q of the bar material by minimizing the difference between the waveform propagated from the shorter bar and the waveform measured within the longer bar. The waveform inversion is performed in the time domain, and the waveforms can be appropriately truncated to avoid multiple reflections due to the finite size of the (shorter) sample, allowing attenuation to be measured at long wavelengths or low frequencies. The frequency range in which this technique operates fills the gap between the resonant bar measurement (∼10 kHz) and ultrasonic measurement (∼100–1000 kHz). By using the technique, attenuation values in a PVC (a highly attenuative) material and in Sierra White granite were measured in the frequency range of 40–140 kHz. The obtained attenuation values for the two materials are found to be reliable and consistent.


2019 ◽  
Vol 219 (2) ◽  
pp. 975-994 ◽  
Author(s):  
Gabriel Gribler ◽  
T Dylan Mikesell

SUMMARY Estimating shear wave velocity with depth from Rayleigh-wave dispersion data is limited by the accuracy of fundamental and higher mode identification and characterization. In many cases, the fundamental mode signal propagates exclusively in retrograde motion, while higher modes propagate in prograde motion. It has previously been shown that differences in particle motion can be identified with multicomponent recordings and used to separate prograde from retrograde signals. Here we explore the domain of existence of prograde motion of the fundamental mode, arising from a combination of two conditions: (1) a shallow, high-impedance contrast and (2) a high Poisson ratio material. We present solutions to isolate fundamental and higher mode signals using multicomponent recordings. Previously, a time-domain polarity mute was used with limited success due to the overlap in the time domain of fundamental and higher mode signals at low frequencies. We present several new approaches to overcome this low-frequency obstacle, all of which utilize the different particle motions of retrograde and prograde signals. First, the Hilbert transform is used to phase shift one component by 90° prior to summation or subtraction of the other component. This enhances either retrograde or prograde motion and can increase the mode amplitude. Secondly, we present a new time–frequency domain polarity mute to separate retrograde and prograde signals. We demonstrate these methods with synthetic and field data to highlight the improvements to dispersion images and the resulting dispersion curve extraction.


Life Sciences ◽  
1998 ◽  
Vol 62 (14) ◽  
pp. 1271-1280 ◽  
Author(s):  
L. Bonhomme-Faivre ◽  
A. Macé ◽  
Y. Bezie ◽  
S. Marion ◽  
G. Bindoula ◽  
...  

2008 ◽  
Vol 6 (1) ◽  
pp. 3 ◽  
Author(s):  
Gabriele Gerardi ◽  
Antonella De Ninno ◽  
Marco Prosdocimi ◽  
Vanni Ferrari ◽  
Filippo Barbaro ◽  
...  

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