Ultra-Bright Fluorescent Organic Nanoparticles Based on Small-Molecule Ionic Isolation Lattices

Ultra-bright fluorescent nanoparticles hold great promise for demanding bioimaging applications. Recently, extremely bright molecular crystals of cationic fluorophores were obtained by hierarchical co-assembly with cyanostar anion-receptor complexes of associated counterions. These small-molecule ionic isolation lattices (SMILES) ensure spatial and electronic isolation to prohibit dye aggregation quenching. We report a simple, one-step supramolecular approach to formulate SMILES materials into nanoparticles. Rhodamine-based SMILES nanoparticles stabilized by glycol amphiphiles show high fluorescence quantum yield (30%) and brightness per volume (5000 M–1 cm–1 / nm3) with 400 dyes packed into 16-nm particles, corresponding to an absorption coefficient of 4 × 107 M–1 cm–1. UV excitation of the cyanostar component leads to highest brightness (>6000 M–1 cm–1 / nm3) by energy transfer to rhodamine emitters. Coated nanoparticles stain cells and are thus promising for bioimaging.

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Calorimetric Sensor for Ethanol Using Ni2+-nitrilotriacetic Acid (NTA) Resin Immobilized Alcohol Dehydrogenase (ADH)

Current Analytical Chemistry ◽

10.2174/1573411015666190617110233 ◽

2020 ◽

Vol 16 (6) ◽

pp. 795-799

Author(s):

YongJin Li

Keyword(s):

Cost Effective ◽

Nitrilotriacetic Acid ◽

Repeated Measurements ◽

Great Promise ◽

Calorimetric Sensor ◽

Controlled Pore Glass ◽

Pore Glass ◽

One Step ◽

Immobilization Matrix ◽

Enzyme Thermistor

Background: A simple, fast and economic analytical method for the determination of ethanol is important for clinical, biological, forensic and physico-legal purposes. Methods: Ni2+-NTA resin was used as an immobilization matrix for the simple one-step purification/ immobilization of his6-tagged ADH. Different alcohols with a concentration range of 0.5-50% V/V, namely methanol, ethanol and propanol were measured using prepared ADH enzyme thermistor. The ethanol content of Tsingtao beer was tested as a real sample containing alcohol. Reproducibility and stability of prepared ADH enzyme thermistor were also investigated by repeated measurements. Results: In comparison to the controlled pore glass (a common used support for the immobilization of enzyme) used in thermal biosensor, the use of Ni2+-NTA resin not only led to simple one-step purification/ immobilization by his6-tagged ADH binding to Ni2+-NTA resin, but also made the immobilizing supports reusable. The prepared biosensor can be used to determine ethanol and methanol by the calorimetric measurement. A linear range of 1 -32% (V/V) and 2-20% (V/V) was observed for ethanol and methanol, respectively. The detection limits were 0.3% (V/V) and 1% (V/V) for ethanol and methanol, respectively. The tested ethanol concentration of Tsingtao beer was 4.5% V/V, which is comparable with the labeled alcohol by volume (ABV) 4.80%. Conclusion: Ni2+-NTA resin, as an immobilization matrix in ET sensor, provides a simple one-step purification/immobilization for His6-tagged recombinase and a reusable immobilization matrix. The prepared biosensor exhibits good repeatability and stability. Such a new biosensor shows great promise for rapid, simple, and cost-effective analysis of ethanol and methanol, both in qualitative and in quantitative tests.

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One step synthesis of silica-coated nanoparticles at room temperature and their luminescence properties

Materials Letters ◽

10.1016/j.matlet.2008.03.017 ◽

2008 ◽

Vol 62 (19) ◽

pp. 3413-3415 ◽

Cited By ~ 5

Author(s):

Yi-lin Wang ◽

Jian-ping Lu ◽

Xiao-feng Huang ◽

Jian-xin Long ◽

Zhang-fa Tong

Keyword(s):

Room Temperature ◽

Luminescence Properties ◽

Coated Nanoparticles ◽

One Step

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Stable Twisted Conformation Aza-BODIPY NIR-II Fluorescent Nanoparticles with Ultra-large Stokes Shift for Imaging-Guided Phototherapy

Journal of Materials Chemistry B ◽

10.1039/d1tb02066h ◽

2022 ◽

Author(s):

Youliang Tian ◽

Huiting Zhou ◽

Quan Cheng ◽

Huiping Dang ◽

Hongyun Qian ◽

...

Keyword(s):

Fluorescence Imaging ◽

In Vivo Imaging ◽

Near Infrared ◽

Stokes Shift ◽

Great Promise ◽

Deep Penetration ◽

Fluorescent Nanoparticles ◽

Spatiotemporal Resolution ◽

Large Stokes Shift

Fluorescence imaging in the second near-infrared window (NIR-II, 1000–1700 nm) holds great promise for in vivo imaging and imaging-guided phototherapy with deep penetration and high spatiotemporal resolution. It is very...

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Highly efficient near-infrared organic dots based on novel AEE fluorogen for specific cancer cell imaging

RSC Advances ◽

10.1039/c5ra04669f ◽

2015 ◽

Vol 5 (46) ◽

pp. 36837-36844 ◽

Cited By ~ 18

Author(s):

Yan Zhang ◽

Kaiwen Chang ◽

Bin Xu ◽

Jinlong Chen ◽

Lulin Yan ◽

...

Keyword(s):

Quantum Efficiency ◽

Cancer Cell ◽

Small Molecule ◽

Near Infrared ◽

Cell Imaging ◽

Amphiphilic Polymer ◽

Cancer Cell Imaging ◽

Highly Efficient ◽

Specific Cancer ◽

High Fluorescence

Near-infrared emissive organic dots with a high fluorescence quantum efficiency (AEE dots) are prepared by using an amphiphilic polymer PSMA and a novel small molecule fluorogen (DPPBPA).

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Disassembling 2D van der Waals crystals into macroscopic monolayers and reassembling into artificial lattices

Science ◽

10.1126/science.aba1416 ◽

2020 ◽

Vol 367 (6480) ◽

pp. 903-906 ◽

Cited By ~ 36

Author(s):

Fang Liu ◽

Wenjing Wu ◽

Yusong Bai ◽

Sang Hoon Chae ◽

Qiuyang Li ◽

...

Keyword(s):

Mass Production ◽

Van Der Waals ◽

Great Promise ◽

Layer By Layer ◽

Transition Metal Dichalcogenide ◽

High Quality ◽

Quantum Devices ◽

Artificial Materials ◽

Two Dimensional Materials ◽

One Step

Two-dimensional materials from layered van der Waals (vdW) crystals hold great promise for electronic, optoelectronic, and quantum devices, but technological implementation will be hampered by the lack of high-throughput techniques for exfoliating single-crystal monolayers with sufficient size and high quality. Here, we report a facile method to disassemble vdW single crystals layer by layer into monolayers with near-unity yield and with dimensions limited only by bulk crystal sizes. The macroscopic monolayers are comparable in quality to microscopic monolayers from conventional Scotch tape exfoliation. The monolayers can be assembled into macroscopic artificial structures, including transition metal dichalcogenide multilayers with broken inversion symmetry and substantially enhanced nonlinear optical response. This approach takes us one step closer to mass production of macroscopic monolayers and bulk-like artificial materials with controllable properties.

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One-step, low-temperature deposited perovskite solar cell utilizing small molecule additive

Journal of Photonics for Energy ◽

10.1117/1.jpe.5.057405 ◽

2015 ◽

Vol 5 (1) ◽

pp. 057405 ◽

Cited By ~ 39

Author(s):

Chun-Chao Chen ◽

Zirou Hong ◽

Gang Li ◽

Qi Chen ◽

Huanping Zhou ◽

...

Keyword(s):

Solar Cell ◽

Low Temperature ◽

Small Molecule ◽

Perovskite Solar Cell ◽

One Step

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Carbohydrate biomarker recognition using synthetic lectin mimics

Pure and Applied Chemistry ◽

10.1351/pac-con-12-04-17 ◽

2012 ◽

Vol 84 (11) ◽

pp. 2479-2498 ◽

Cited By ~ 13

Author(s):

Chaofeng Dai ◽

Arpana Sagwal ◽

Yunfeng Cheng ◽

Hanjing Peng ◽

Weixuan Chen ◽

...

Keyword(s):

Nucleic Acid ◽

Small Molecule ◽

Targeted Delivery ◽

Boronic Acid ◽

Peptide Nucleic Acid ◽

Therapeutic Agents ◽

Imaging Agents ◽

Great Promise ◽

Wide Range ◽

Tremendous Amount

Carbohydrate biomarkers play very important roles in a wide range of biological and pathological processes. Compounds that can specifically recognize a carbohydrate biomarker are useful for targeted delivery of imaging agents and for development of new diagnostics. Furthermore, such compounds could also be candidates for the development of therapeutic agents. A tremendous amount of active work on synthetic lectin mimics has been reported in recent years. Amongst all the synthetic lectins, boronic-acid-based lectins (boronolectins) have shown great promise. Along this line, four classes of boronolectins including peptide-, nucleic-acid-, polymer-, and small-molecule-based ones are discussed with a focus on the design principles and recent advances. We hope that by presenting the potentials of this field, this review will stimulate more research in this area.

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A Precised Surface Modification of Hydroxyapatite with Poly(methylmethacrylate) for Tissue Engineering & Regenerative Medicine

Asian Journal of Chemistry ◽

10.14233/ajchem.2019.21616 ◽

2019 ◽

Vol 31 (3) ◽

pp. 545-550

Author(s):

Trinh Duy Nguyen ◽

Phu Thuong Nhan Nguyen ◽

Thien Hien Tran ◽

Md. Rafiqul Islam ◽

Kwon Taek Lim ◽

...

Keyword(s):

Tissue Engineering ◽

Regenerative Medicine ◽

Radical Polymerization ◽

Colloidal Stability ◽

Hybrid Nanocomposites ◽

Great Promise ◽

Dsc Studies ◽

One Step ◽

Grafting From ◽

Hydroxyapatite Nanocrystals

The poly(methylmethacrylate) (PMMA) grafted biocompatible hydroxyapatite nanocrystals (HAPs) hybrid nanocomposites (PMMA-g-HAPs) were synthesized by employing surface thiol-lactam initiated radical polymerization (TLIRP) through grafting from strategy. At first, the surface of HAPs was functionalized by 3-mercaptopropyl-trimethoxysilane in one-step process to prepare thiol immobilized HAPs (HAPs-SH). Subsequently, a controlled radical polymerization of MMA by using two component initiating system comprising of HAPs-SH and butyrolactam (BL) successfully afforded PMMA-g-HAPs nanocomposites. The resulting structure and morphological feature of nanocomposites was systematically characterized by FT-IR and XRD analyses. GPC studies of cleaved polymers from nanocomposites of different time revealed that the grafting polymerization from the surface of HAP was well controlled in nature. Moreover, the thermal property of the PMMA was found to be improved by incorporation of inorganic HAP nanoparticles in the polymer matrix as revealed by TGA and DSC studies. The colloidal stability of the synthesized nanocomposites was observed to be exceptionally good in organic solvents as suggested by the time dependent monitoring using UV-visible spectroscopy and captured digital photographs. The synthesized nanocomposites show a great promise for the safe application in tissue engineering and regenerative medicine.

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Fabrication of amphiphilic fluorescent nanoparticles with an AIE feature via a one-pot clickable mercaptoacetic acid locking imine reaction: synthesis, self-assembly and bioimaging

Polymer Chemistry ◽

10.1039/c6py00851h ◽

2016 ◽

Vol 7 (27) ◽

pp. 4559-4566 ◽

Cited By ~ 23

Author(s):

Qing Wan ◽

Meiying Liu ◽

Dazhuang Xu ◽

Liucheng Mao ◽

Hongye Huang ◽

...

Keyword(s):

Self Assembly ◽

Mercaptoacetic Acid ◽

Fluorescent Nanoparticles ◽

Reaction Synthesis ◽

One Pot ◽

Organic Nanoparticles ◽

Water Dispersible ◽

Fluorescent Organic Nanoparticles

Water dispersible and non-toxic AIE active fluorescent organic nanoparticles were fabricatedviaa one-pot clickable mercaptoacetic acid locking imine reaction.

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