scholarly journals A Multiple-Technique Approach to Inferring Bio-electrochemical Reaction Parameters

Author(s):  
Henry Lloyd-Laney ◽  
Nick Yates ◽  
Martin Robinson ◽  
Alice R. Hewson ◽  
Alison Parkin ◽  
...  

Uncovering the secrets of the biological Faradaic reactions, essential to the understanding of complex metalloenzymes, requires an information recovery process that is robust, rapid and replicable. This paper is a description of the workflow we have developed over the course of inferring chemical reaction parameters for a simple protein system, a bacterial cytochrome domain from \textit{Cellvibrio japonicus}. This was a challenging task, as the signal-to-noise ratio in such protein-film voltammetry experiments is significantly lowered relative to the voltammetric data generated by simple chemicals. We have overcome these challenges by using a multiple-technique approach, which compensates for the difficulties inherent to analysis of the individual voltammetry experiments. We have shown that the parameters inferred are robust across multiple experiments performed for different preperations of the protein. This is an important proof-of-concept result for analysis of more complex metalloenzymes, which incorporate catalytic processes and multiple internal electron-transfer sites. <br>

2021 ◽  
Author(s):  
Henry Lloyd-Laney ◽  
Nick Yates ◽  
Martin Robinson ◽  
Alice R. Hewson ◽  
Alison Parkin ◽  
...  

Uncovering the secrets of the biological Faradaic reactions, essential to the understanding of complex metalloenzymes, requires an information recovery process that is robust, rapid and replicable. This paper is a description of the workflow we have developed over the course of inferring chemical reaction parameters for a simple protein system, a bacterial cytochrome domain from \textit{Cellvibrio japonicus}. This was a challenging task, as the signal-to-noise ratio in such protein-film voltammetry experiments is significantly lowered relative to the voltammetric data generated by simple chemicals. We have overcome these challenges by using a multiple-technique approach, which compensates for the difficulties inherent to analysis of the individual voltammetry experiments. We have shown that the parameters inferred are robust across multiple experiments performed for different preperations of the protein. This is an important proof-of-concept result for analysis of more complex metalloenzymes, which incorporate catalytic processes and multiple internal electron-transfer sites. <br>


2003 ◽  
Vol 125 (50) ◽  
pp. 15352-15358 ◽  
Author(s):  
Kondo François Aguey-Zinsou ◽  
Paul V. Bernhardt ◽  
Silke Leimkühler

Energies ◽  
2021 ◽  
Vol 14 (8) ◽  
pp. 2300
Author(s):  
Bronisław Andrzej Kolator

In this paper, the energy diagnostic of tractor performance consists in evaluating the energy (fuel consumption per hectare—dm3 ha−1) for a given agricultural operation and in combining it with working capacity, also called productivity (area productivity—ha h−1). One of the methods of solving this problem is the identification of the functioning process of the machine unit. A model of the process of the machine unit performance was developed, considering the operation of the rear linkage system of the implement with the force control adjustment system. In order to analyze the system, a mathematical model of the system function was built: tractor-implement-soil, defining the physical connections and interdependencies between the individual subsystems of the system. Based on this model, a simulation model was developed and implemented in the Matlab/Simulink environment. The Simulink package was used to test the performance of the machine set. The efficiency indicators according to the adopted criteria were calculated in the evaluation block. To evaluate the process, the technical and operational parameters of the tractor, the type and parameters of the tool, and soil properties were taken into account. The results of simulation studies obtained on a validated model are consistent with experimental data from appropriate soil conditions.


2018 ◽  
Vol 8 (10) ◽  
pp. 1954 ◽  
Author(s):  
Xing-Ting Xiong ◽  
Xing-Hua Qu ◽  
Fu-Min Zhang

In our frequency scanning interferometry-based (FSI-based) absolute distance measurement system, a frequency sampling method is used to eliminate the influence of laser tuning nonlinearity. However, because the external cavity laser (ECL) has been used for five years, factors such as the mode hopping of the ECL and the low signal-to-noise ratio (SNR) in a non-cooperative target measurement bring new problems, including erroneous sampling points, phase jumps, and interfering signals. This article analyzes the impacts of the erroneous sampling points and interfering signals on the accuracy of measurement, and then proposes an adaptive filtering method to eliminate the influence. In addition, a phase-matching mosaic algorithm is used to eliminate the phase jump, and a segmentation mosaic algorithm is used to improve the data processing speed. The result of the simulation proves the efficiency of our method. In experiments, the measured target was located at eight different positions on a precise guide rail, and the incident angle was 12 degrees. The maximum deviation of the measured results between the FSI-based system and the He-Ne interferometer was 9.6 μm, and the maximum mean square error of our method was 2.4 μm, which approached the Cramer-Rao lower bound (CRLB) of 0.8 μm.


2009 ◽  
Vol 20 (3) ◽  
pp. 253-273 ◽  
Author(s):  
Stefan Michel ◽  
David Bowen ◽  
Robert Johnston

PurposeThe keys to effective service recovery are familiar to many throughout industry and academia. Nevertheless, overall customer satisfaction after a failure has not improved, and many managers claim their organizations cannot respond to and fix recurring problems quickly enough. Why does service recovery so often fail and what can managers do about it? This paper aims to address these issues.Design/methodology/approachThe objective is to produce an interdisciplinary summary of the growing literature on service recovery, bringing together what each of the author's domain – management, marketing, and human resources management – has to offer. By contrasting those three perspectives using 141 academic sources, nine tensions between customer, process, and employee recovery are discovered.FindingsIt is argued that service recovery often fails due to the unresolved tensions found between the conflicting perspectives of customer recovery, process recovery, and employee recovery. Therefore, successful service recovery requires the integration of these different perspectives. This is summarized in the following definition: “Service recovery are the integrative actions a company takes to re‐establish customer satisfaction and loyalty after a service failure (customer recovery), to ensure that failure incidents encourage learning and process improvement (process recovery) and to train and reward employees for this purpose (employee recovery).”Practical implicationsManagers are not advised to directly address and solve the nine tensions between customer recovery, process recovery, and employee recovery. Instead, concentrating on the underlying cause of these tensions is recommended. That is, managers should strive to integrate service recovery efforts based upon a “service logic”; a balance of functional subcultures; strategy‐driven resolution of functional differences; data‐based decision making from the seamless collection and sharing of information; recovery metrics and rewards; and development of “T‐shaped” employees with a service, not just functional, mindset.Originality/valueThis paper provides an interdisciplinary view of the difficulties to implement a successful service recovery management. The contribution is twofold. First, specific tensions between customer, process and employee recovery are identified. Second, managers are offered recommendations of how to integrate the diverging perspectives.


Author(s):  
Ada Scupola

This article investigates the competences deemed necessary both at top managerial and individual levels for the successful adoption and assimilation of business-to-business e-services in small and medium size enterprises. To this end, an in-depth case study of a business-to-business e-service system, a Web-based travel reservation system, was conducted. The results show that three main competences, namely vision, value and control, are important at top management level for the primary adoption of e-services. For secondary adoption and assimilation, three categories of competences were identified as being important either to have or to develop at the individual level, namely technical, interpersonal and conceptual skills.


Author(s):  
В. Б. Швайченко ◽  
О. П. Гребінь ◽  
Н. Ф. Левенець

Improving the quality of the restored information in the process of restoration and restoration of phonograms.Synthesis of the system model on the basis of analysis of the processes of restoration and restoration of phonograms from media of various types and computer processing. The characteristics of the conceptual model of the restoration and restoration of the phonogram are determined. The structure of the system model of the information recovery process is developed. A lot of concepts and connections between concepts are defined. The structure of the system modeling restoration and restoration of phonograms is defined. A conceptual model of the restoration and restoration process is proposed. The distribution of artifacts over the playback and processing modes of a phonogram is justified. Details of the type of content with features of the effect on the state of the phonogram.The solutions obtained are the basis of the methodology for carrying out the process of restoration and restoration of phonograms by the criterion of sound quality.


2021 ◽  
Author(s):  
Nguyen Thi Yen Linh ◽  
Tu Ngo Hoang ◽  
Pham Ngoc Son ◽  
Vo Nguyen Quoc Bao

<div>This paper investigates short-packet communications for the dual-hop decode-and-forward relaying system to facilitate ultra-reliable and low-latency communications. In this system, a selected relay having the highest signal-to-noise ratio (SNR) serves as a forwarder to support the unavailable direct link between the source and destination, whereas a maximum ratio combining technique is leveraged at the destination to achieve the highest diversity gain. Approximated expressions of end-to-end (e2e) block error rates (BLERs) are derived over quasi-static Rayleigh fading channels and the finite-blocklength regime. To gain more insights about the performance behavior in the high-SNR regime, we provide the asymptotic analysis with two approaches, from which the qualitative conclusion based on the diversity order is made. Furthermore, the power allocation and relay location optimization problems are also considered to minimize the asymptotic e2e BLER under the configuration constraints. Our analysis is verified through Monte-Carlo simulations, which yield the system parameters' impact on the system performance.</div>


2020 ◽  
Author(s):  
O. Vershinina ◽  
M. Ivanchenko ◽  
M.G. Bacalini ◽  
A. Zaikin ◽  
C. Franceschi

ABSTRACTDNA methylation variability arises due to concurrent genetic and environmental influences. Each of them is a mixture of regular and noisy sources, whose relative contribution has not been satisfactorily understood yet. We conduct a systematic assessment of the age-dependent methylation by the signal-to-noise ratio and identify a wealth of “deterministic” CpG probes (about 90%), whose methylation variability likely originates due to genetic and general environmental factors. The remaining 10% of “stochastic” CpG probes are arguably governed by the biological noise or incidental environmental factors. Investigating the mathematical functional relationship between methylation levels and variability, we find that in about 90% of the age-associated differentially methylated positions, the variability changes as the square of the methylation level, whereas in the most of the remaining cases the dependence is linear. Furthermore, we demonstrate that the methylation level itself in more than 15% cases varies nonlinearly with age (according to the power law), in contrast to the previously assumed linear changes. Our findings present ample evidence of the ubiquity of strong DNA methylation regulation, resulting in the individual age-dependent and nonlinear methylation trajectories, whose divergence explains the cross-sectional variability. It may also serve a basis for constructing novel nonlinear epigenetic clocks.


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