Comparative Evaluation Of Antibacterial And Layer Removal Efficacy Of Two Different Herbal Irrigants – An In Vitro Study

INTRODUCTION: Irrigation during root canal therapy is especially needed for teeth with complex internal anatomy. Biomechanical preparation of root canal produces "smear layer" which gets embedded with debris and microorganisms. The major side effects of these widely used chemical irrigants such as Sodium hypochlorite for antibacterial efficacy and EDTA for smear layer removal has led to the search of a more biocompatible irrigant.MATERIALS AND METHODS: Azadirachta indica(neem) and Ocimum sanctum(tulsi) extract were used in this study as herbal irrigants to evaluate the above mentioned properties by the q PCR method and SEM analysis.RESULTS: In antimicrobial efficacy, the Mean value of NaOcl showed higher antimicrobial efficacy followed by Neem leaf extract and the lower antimicrobial efficacy was recorded with Tulsi extract. Similarly, the mean smear layer removal by different irrigants at different root levels, EDTA showed the maximum smear layer removal, followed by Neem leaf extract and Tulsi extract showed the least smear layer removal. Hence Tulsi has poor smear layer removal efficacy and comparable antimicrobial efficacy when compared with Neem extractSTATISTICAL ANALYSIS: The collected data were analyzed by one-way analysis of variance to compare the mean of the groups. The post- hoc test (Tukey) was performed to find the interrelationship between different groups for significant difference (P < 0.05; confidence interval 95%).CONCLUSION: From this study, these two herbal irrigants are proved to be safe and effective. It can be concluded that neem leaf extract has a significant antimicrobial efficacy against E. faecalis and significant smear layer removal efficacy compared to 17% EDTA.

Download Full-text

In Vitro Evaluation of Different Irrigation Protocols on Intracanal Smear Layer Removal in Teeth with or without Pre-Endodontic Proximal Wall Restoration

Journal of Clinical Medicine ◽

10.3390/jcm9103325 ◽

2020 ◽

Vol 9 (10) ◽

pp. 3325

Author(s):

Naji Kharouf ◽

Eugenio Pedullà ◽

Giusy Rita Maria La Rosa ◽

Frédéric Bukiet ◽

Salvatore Sauro ◽

...

Keyword(s):

Root Canal ◽

Resin Composite ◽

Smear Layer ◽

Canal Irrigation ◽

Layer Removal ◽

Apical Level ◽

Root Canal Irrigation ◽

Significant Difference ◽

Sonic Activation

To investigate the influence of pre-endodontic coronal wall restoration on smear layer removal during different root canal irrigation strategies, single-root premolars were prepared with a mesio-occlusal cavity. Half were left untreated (G1), while the mesial walls of the remaining half were reconstructed using a resin composite (G2). The specimens were divided into control (ctrl) groups, which used the conventional needle irrigation method, and four experimental subgroups according to irrigation strategy: Sonic activation using the Endoactivator, sonic activation using the EQ-S, mechanical activation using the XP-Endo finisher, and ultrasonic activation using the EndoUltra. Smear layer removal was assessed through SEM and the results were statistically analyzed. At the coronal and middle thirds, no significant difference (p > 0.05) was detected for G1 and G2, except for the control subgroups (G1ctrl vs G2 ctrl) at the middle third. At the apical third, the smear layer removal was significantly greater for G2 than G1. In G1, both at the middle and apical level, EQ-S and EndoUltra showed greater smear layer removal (p < 0.05) compared to the others G1 subgroups. In G2, at the apical level, the EQ-S and EndoUltra were the most effective in smear layer removal. Pre-endodontic coronal wall restoration may improve the smear layer removal during root canal irrigation.

Download Full-text

The Effectiveness of Three Different Plant Extracts Used as Irrigant in Removal of Smear Layer: A Scanning Electron Microscope Study

Journal of Oral Health and Community Dentistry ◽

10.5005/johcd-9-1-16 ◽

2015 ◽

Vol 9 (1) ◽

pp. 16-22 ◽

Cited By ~ 1

Author(s):

A Gupta ◽

V Goyal ◽

J Duhan ◽

S Hans ◽

P Sangwan

Keyword(s):

Plant Extracts ◽

Root Canal ◽

Smear Layer ◽

Ocimum Sanctum ◽

Control Group ◽

Syzygium Aromaticum ◽

Distilled Water ◽

Scanning Electron Microscope Study ◽

Layer Removal ◽

Scanning Electron

ABSTRACT Aim In the present study, the role of three plant extracts as irrigant in root canal cleaning after instrumentation was evaluated. The effect of Syzygium aromaticum (S. Aromaticum), Ocimum sanctum (O. Sanctum) and Cinnamomum zeylanicum (C. zeylanicum) plant extracts was evaluated in smear layer removal. Methods The study was divided into different groups having 5 teeth each using various irrigating agents to evaluate smear layer removal. Group A: O. Sanctum extract; group A1: O. Sanctum extract with EDTA, group B: S. Aromaticum extract; group B1 S. Aromaticum extract with EDTA, group C: C. zeylanicum extract; group C1 C. zeylanicum extract with EDTA and two control group of 5 teeth each in group D: 3% NaOCl; group D1 3% NaOCl with EDTA (as positive control) and group E: Distilled water (as negative control); group E1 3% Distilled water with EDTA. Each tooth was split longitudinally and prepared for examination by scanning electron microscopy. Results The herbal extracts were effective in cleaning root canal walls when combine with EDTA with maximum activity of S. Aromaticum extract with EDTA group. Conclusion Under the condition of present study the three herbal plant extracts were ineffective in removal of smear layer when used alone.

Download Full-text

A Comparative Evaluation of Cleaning Efficacy (Debris and Smear Layer Removal) of Hand and Two NiTi Rotary Instrumentation Systems (K3 and ProTaper): A SEM Study

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1445 ◽

2013 ◽

Vol 14 (6) ◽

pp. 1028-1035

Author(s):

Sangeetha Vallikanthan ◽

K Balakoti Reddy ◽

Shreemoy Dash ◽

Sowmya Kallepalli ◽

N Chakrapani ◽

...

Keyword(s):

Root Canal ◽

Comparative Evaluation ◽

Group Iv ◽

Smear Layer ◽

Layer Removal ◽

Group I ◽

Rotary Instruments ◽

Significant Difference ◽

Sem Study ◽

Cleaning Efficacy

ABSTRACT Objectives The present study was conducted to compare the cleaning efficacy (debris and smear layer removal) of hand and two NiTi rotary instrumentation systems (K3 and ProTaper). Materials and methods Sixty single rooted human maxillary anterior teeth decoronated at the cementoenamel junction were used. All the specimens were divided into four groups of 15 teeth each, group I—ProTaper rotary instrumentation done, group II—K3 rotary instrumentation done, group III—Stainless steel K-file instrumentation done, group IV—root canal irrigation without instrumentation. Root canal preparation was done in a crown down manner and 3% sodium hypochlorite was used as irrigant after each file followed by final rinse with 5 ml of 17% EDTA solution, then specimens were scanning electron microscopic (SEM) examination. Results Statistical analysis was done using one-way ANOVA followed by post hoc Tukey's HSD test. Group I showed highly statistical significant difference compared to other groups. There was no statistically significant difference considering smear layer at any levels among the groups with no smear layer formation in group IV. Conclusion ProTaper rotary instrumentation showed the maximum cleaning efficacy followed by K3 rotary instrumentation in the coronal, middle and apical thirds of the root canal. Clinical significance ProTaper rotary instruments are more efficient than hand and K3 rotary instruments during root canal treatment. How to cite this article Reddy KB, Dash S, Kallepalli S, Vallikanthan S, Chakrapani N, Kalepu V. A Comparative Evaluation of Cleaning Efficacy (Debris and Smear Layer Removal) of Hand and Two NiTi Rotary Instrumentation Systems (K3 and ProTaper): A SEM Study. J Contemp Dent Pract 2013;14(6):1028-1035.

Download Full-text

Evaluation of four final irrigation protocols for cleaning root canal walls

International Journal of Oral Science ◽

10.1038/s41368-020-00091-4 ◽

2020 ◽

Vol 12 (1) ◽

Author(s):

Qiang Li ◽

Qian Zhang ◽

Xiaoying Zou ◽

Lin Yue

Keyword(s):

Root Canal ◽

Laser Scanning ◽

Laser Scanning Microscopy ◽

Smear Layer ◽

Confocal Laser ◽

Bacterial Survival ◽

Bacterial Inhibition ◽

Layer Removal ◽

Significant Difference ◽

Canal Systems

Abstract The aim of this study was to compare the efficiency of four final irrigation protocols in smear layer removal and bacterial inhibition in root canal systems. Thirty roots inoculated with Enterococcus faecalis were prepared with ProTaper Universal files. The teeth were disinfected by conventional needle irrigation, sonic agitation using the EndoActivator device, passive ultrasonic irrigation, or an M3 Max file. Teeth with no root canal preparation served as blank controls for the establishment of the infection baseline. Teeth with preparation but no final irrigation served as a post-instrumentation baseline. After the final irrigation, the teeth were sectioned in half. One half of each tooth was examined by scanning electron microscopy (SEM) to assess smear layer removal using a five-point scale. The other half was examined by confocal laser scanning microscopy (CLSM) using the LIVE/DEAD BackLight bacterial viability kit to evaluate the depth of bacterial survival in dentinal tubules. SEM analysis revealed no significant difference in smear layer removal throughout the whole canal among the EA, PUI, and M3 Max groups (P > 0.05). CLSM revealed that PUI achieved the greatest bacterial inhibition depth in the coronal ((174.27 ± 31.63) μm), middle ((160.94 ± 37.77) μm), and apical ((119.53 ± 28.49) μm) thirds of the canal (all P < 0.05 vs. other groups). According to this comprehensive SEM and CLSM evaluation, PUI appears to have the best infection control ability in root canal systems.

Download Full-text

An in-vitro study of smear layer removal and microbial leakage along root-canal fillings

International Endodontic Journal ◽

10.1111/j.1365-2591.1996.tb01169.x ◽

1996 ◽

Vol 29 (2) ◽

pp. 99-107 ◽

Cited By ~ 45

Author(s):

G. D. BEHREND ◽

C. W. CUTLER ◽

J. L. GUTMANN

Keyword(s):

Root Canal ◽

In Vitro Study ◽

Smear Layer ◽

Vitro Study ◽

Layer Removal

Download Full-text

Comparison Between Different Endodontic Irrigating Protocols In Smear Layer Removal From Radicular Dentin

European Scientific Journal ESJ ◽

10.19044/esj.2016.v12n15p38 ◽

2016 ◽

Vol 12 (15) ◽

pp. 38

Author(s):

Ramona Vlad ◽

Monika Kovacs ◽

Dragos Sita ◽

Mihai Pop

Keyword(s):

Root Canal ◽

Endodontic Treatment ◽

Smear Layer ◽

Friedman Test ◽

Cleaning Efficiency ◽

Layer Removal ◽

Significant Difference ◽

System Data ◽

Root Canal Dentin ◽

Level Of Significance

The aim of our study was to measure the cleaning efficiency of irrigating solutions used during endodontic treatment regarding smear layer removal from the root canal dentin walls. Ethylenediaminotetraacetic acid (EDTA) 17%, citric acid (CA) 10% and sodium hypochlorite (NaOCl) 2,5 % solutions were tested as final irrigating solutions. The study was conducted on extracted teeth, divided in four groups according to the irrigation protocol used. The specimens were analyzed by scanning electron microscopy and the amount of smear layer present at apical, middle and coronal level was recorded, based on a scoring system. Data were statistically analyzed using Kruskal-Wallis and Friedman test and the level of significance was set at p<0.05. In the coronal and middle segments of dental roots we noticed no statistically significant difference between EDTA and CA in smear layer removing capacity. Final irrigation with 17% EDTA proved to be more efficient than 10% CA and 2,5% NaOCl in smear layer removal at apical level of the root canal, with p<0.05 (p=0.042), which is an important area for disinfection in endodontic treatment.

Download Full-text