scholarly journals Ratio spectrum derivative quantitative analysis of propranolol and diazipam in combind pharmaceutical mixtures

2019 ◽  
Vol 10 (4) ◽  
pp. 3657-3664 ◽  
Author(s):  
Suhad Abdel Moneim Yassin ◽  
Khalaf F Al-Samarrai
Keyword(s):  
Quantitative Analysis ◽  
Spectrophotometric Methods ◽  
Ratio Spectrum ◽  
Analytical Results ◽  
Derivative Method

Two new spectrophotometric methods were developed for the simultaneously estimation of DZP and PRO using the first ratio derivative method (DD1) and the second ratio derivative (DD2). The two methods were succeeded to the estimation of both drugs with a range of concentrations from (5-50 µg.ml-1), depending on Peak to baseline, peak area, peak to peak at specific wavelengths for each compound. The analytical results and of the estimate of The DZP and PRO showed that Rec% values ranged from 95.04-104.97% and 96.30-104.69, and RSD% were between 0.00948-4.87736 % and 0.1163-3.37655%, for the first and second ratio derivative respectively. The method has been successfully applied to the estimation of both PRO and DZP in its pharmaceuticals forms.

Quantitative Analysis of Binary Mixtures. I. General Method

1981 ◽  
Vol 35 (4) ◽  
pp. 421-428 ◽  
Author(s):  
Hugh E. Diem ◽  
Samuel Krimm
Keyword(s):  
Quantitative Analysis ◽  
Binary Mixtures ◽  
Spectroscopic Method ◽  
Scaling Factors ◽  
External Calibration ◽  
Original Mixture ◽  
Analytical Results ◽  
Fractionation Procedure ◽  
General Method

A general spectroscopic method for the analysis of binary mixtures which does not require external calibration has been developed. Spectra of the original mixture and of the mixture following some fractionation procedure are obtained. The method is based on the scaling factors required to null the components, one at a time, in successive absorbance subtractions of the two spectra. The accuracy is improved by a factor of approximately ten more than that of an earlier method. The effect of errors in the scaling factors on the analytical results is considered.

Simultaneous Estimation of Ofloxacin, Clotrimazole, and Lignocaine Hydrochloride in Their Combined Ear-Drop Formulation by Two Spectrophotometric Methods

2017 ◽  
Vol 100 (1) ◽  
pp. 38-44
Author(s):  
Kunjan Bodiwala ◽  
Shailesh Shah ◽  
Yogini Patel ◽  
Pintu Prajapati ◽  
Bhavin Marolia ◽  
...  
Keyword(s):  
Dosage Form ◽  
Correlation Coefficients ◽  
The Other ◽  
Simultaneous Estimation ◽  
Standard Mixture ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
Derivative Spectra ◽  
Derivative Method ◽  
Ear Drops

Abstract Two sensitive, accurate, and precise spectrophotometric methods have been developed and validated for the simultaneous estimation of ofloxacin (OFX), clotrimazole (CLZ), and lignocaine hydrochloride (LGN) in their combined dosage form (ear drops) without prior separation. The derivative ratio spectra method (method 1) includes the measurement of OFX and CLZ at zero-crossing points (ZCPs) of each other obtained from the ratio derivative spectra using standard LGN as a divisor, whereas the measurement of LGN at the ZCP of CLZ is obtained from the ratio derivative spectra using standard OFX as a divisor. The double divisor-ratio derivative method (method 2) includes the measurement of each drug at its amplitude in the double divisor-ratio spectra obtained using a standard mixture of the other two drugs as the divisor. Both methods were found to be linear (correlation coefficients of >0.996) over the ranges of 3–15, 10–50, and 20–100 μg/mL for OFX, CLZ, and LGN, respectively; precise (RSD of <2%); and accurate (recovery of >98%) for the estimation of each drug. The developed methods were successfully applied for the estimation of these drugs in a marketed ear-drop formulation. Excipients and other ingredients did not interfere with the estimation of these drugs. Both methods were statistically compared using the t-test.

Different Spectrophotometric and Chromatographic Methods for Determination of Mepivacaine and Its Toxic Impurity

2017 ◽  
Vol 100 (5) ◽  
pp. 1392-1399 ◽  
Author(s):  
Nada S Abdelwahab ◽  
Nehal F Fared ◽  
Mohamed Elagawany ◽  
Esraa H Abdelmomen
Keyword(s):  
Particle Size ◽  
Stationary Phase ◽  
Second Derivative ◽  
Spectrophotometric Measurement ◽  
Spectrophotometric Methods ◽  
Derivative Method ◽  
Tlc Plates ◽  
Second Derivative Method ◽  
Developing System

Abstract Stability-indicating spectrophotometric, TLC-densitometric, and ultra-performance LC (UPLC) methods were developed for the determination of mepivacaine HCl (MEP) in the presence of its toxic impurity, 2,6-dimethylanaline (DMA). Different spectrophotometric methods were developed for the determination of MEP and DMA. In a dual-wavelength method combined with direct spectrophotometric measurement, the absorbancedifference between 221.4 and 240 nm was used for MEPmeasurements, whereas the absorbance at 283 nm was used for measuring DMA in the binary mixture. In the second-derivative method, amplitudes at 272.2 and 232.6 nm were recorded and used for the determination of MEP and DMA, respectively. The developed TLC-densitometric method depended on chromatographic separation using silica gel 60 F254 TLC plates as a stationary phase and methanol–water–acetic acid (9 + 1 + 0.1, v/v/v) as a developing system, with UV scanning at 230 nm. The developed UPLC method depended on separation using a C18 column (250 × 4.6 mm id, 5 μm particle size) as a stationary phase and acetonitrile–water (40 + 60, v/v; pH 4 with phosphoric acid) as a mobilephase at a flow rate of 0.4 mL/min, with UV detection at 215 nm. The chromatographic run time was approximately 1 min. The proposed methods were validated with respect to International Conference on Harmonization guidelines regarding precision, accuracy, ruggedness, robustness, and specificity.

scholarly journals Novel Spectrophotometric Methods for the Quantitative Analysis of Rufinamide in Pharmaceutical Dosage Forms

2012 ◽  
Vol 2 (1) ◽  
pp. 13-18 ◽  
Author(s):  
M. MATHRUSRI ANNAPURNA ◽  
◽  
G.SIVARAMA KRISHNA ◽  
B. PADMAKAR ◽  
B.SATYA KIRAN
Keyword(s):  
Quantitative Analysis ◽  
Dosage Forms ◽  
Pharmaceutical Dosage Forms ◽  
Spectrophotometric Methods

scholarly journals Analysis of Ambroxol Hydrochloride in Flavamed® Tablets by Means of Spectroscopic Absorption Methods

2016 ◽  
Vol 13 (1-2) ◽  
Author(s):  
Dijana Jelić ◽  
Saida Fazlagić ◽  
Vesna Antunović ◽  
Nataša Bubić-Pajić ◽  
Anđelka Račić ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Quantitative Analysis ◽  
Atomic Absorption ◽  
Absorption Spectroscopy ◽  
Atomic Absorption Spectroscopy ◽  
Active Component ◽  
Drug Analysis ◽  
Spectrophotometric Methods ◽  
Synthetic Derivative ◽  
Ambroxol Hydrochloride

Ambroxol hydrochloride (AMB), (1s,4s)-4-((2-amino-3,5-dibromocyclohexyl)methylamino) cyclohexanol hydrochloride, is semi - synthetic derivative of vasicine obtained from Indian shrub Adhatoda vasica. It is a metabolic product of bromhexin and it is used as broncho secretolytic and an expectorant drug. Analysis of Flavamed® tablets, in which ambroxol hydrochloride (AMB) is an active component, was performed. UV/VIS spectrophotometry and atomic absorption spectroscopy (AAS) were used. Direct and indirect UV/VIS spectrophotometric methods were used for quantitative analysis of AMB and the following recovery value results were obtained: 100,16% and 95,23%, successively. Content of heavy metals in Flavamed® tablets was determined by atomic absorption spetroscopy.

scholarly journals Derivative Zero - Crossing Spectrophotometry Of Binary Mixtures Of Acetyl Acetonates Of Micro – Amounts Of Mn (Ii) And Al (Iii).

2013 ◽  
Vol 10 (3) ◽  
pp. 866-882
Author(s):  
Baghdad Science Journal
Keyword(s):  
Binary Mixtures ◽  
Measurement Technique ◽  
Derivative Spectrophotometry ◽  
Molecular Absorption ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
Analytical Results

New derivative molecular absorption spectrophotometric methods have been developed for the determination of Al (III) , Mn (II) , individually and binary mixtures . The aim of this model of study is to obtain analytical results characterized by adequate standard of analytical figures of merits through application of derivative Spectrophotometry (dnA/d?n). The two metals acetyl acetonates are chemically stable and are widely used as catalysts . Where Interferences are probable due to very close or nearby peaks or Summits, the Zero – Crossing derivative measurement technique is used to avoid interfering effects between two metals pairs.

scholarly journals Flow injection analysis of vancomycin

2018 ◽  
Vol 33 (2) ◽  
pp. 67
Author(s):  
Marta Maria Duarte Carvalho Vila ◽  
Acacia Adriana Salomao ◽  
Matthieu Tubino
Keyword(s):  
Quantitative Analysis ◽  
Detection Limit ◽  
Flow Injection ◽  
Flow Injection Analysis ◽  
Copper Ions ◽  
Complete Agreement ◽  
Significance Level ◽  
Statistical Comparison ◽  
Spectrophotometric Methods ◽  
Quantitation Limit

A flow injection method for the quantitative analysis of vancomycin hydrochloride, C66H75Cl2N9O24.HCl (HVCM), based on the reaction with copper (II) ions, is presented. HVCM forms a lilac-blue complex with copper ions at pH≅4.5 in aqueous solutions, with maximum absorption at 555 nm. The detection limit was estimated to be about 8.5×10-5 mol L-1; the quantitation limit is about 2.5×10-4 mol L-1 and about 30 determinations can be performed in an hour. The accuracy of the method was tested through recovery procedures in presence of four different excipients, in the proportion 1:1 w/w. The results were compared with those obtained with the batch spectrophotometric and with the HPLC methods. Statistical comparison was done using the Student’s procedure. Complete agreement was found at a 0.95 significance level between the proposed flow injection and the batch spectrophotometric methods, which present similar precision (RSD: 2.1 % vs. 1.9%).

scholarly journals Two Derivative Spectrophotometric Methods for the Simultaneous Determination of 4-AminoAntipyrine in Presence of Its Acidic Products

2018 ◽  
Vol 31 (1) ◽  
pp. 114
Author(s):  
Ruba Fahmi Abbas
Keyword(s):  
Correlation Coefficient ◽  
Limit Of Detection ◽  
Coefficient Of Determination ◽  
Second Derivative ◽  
Zero Crossing ◽  
Spectrophotometric Methods ◽  
Ratio Spectrum ◽  
First Derivative ◽  
Normal Ratio ◽  
Recovery Percentage

    Simple, economic and sensitive mathematical spectrophotometric methods were developed for the estimation 4-aminoantipyrine in presence of its acidic product. The estimation of binary mixture 4-aminoantipyrine and its acidic product was achieved by first derivative and second derivative spectrophotometric methods by applying zero-crossing at (valley 255.9nm and 234.5nm) for 4-aminoantipyrine and (peak 243.3 nm and 227.3nm) for acidic product. The value of  coefficient of determination  for the liner  graphs were  not less than 0.996 and the recovery percentage were found to be in the range from 96.555 to 102.160. Normal ratio spectrophotometric method 0DD was used 50 mg/l acidic product as a divisor and then measured at 299.9 nm with correlation coefficient 0.998 and limit of detection 0.04098. ratio derivative methods 1DD and 2DD; are based on measuring the first derivative and second derivative for normal ratio spectrum at (peak 290.7 nm and valley 310 nm) for 1DD and(peak 286, valley 301 and peak 316nm) for 2DD the correlation coefficient for linearity graph not less than 0.997 and the recovery percentage were found to be in the range from 99.64 to 100.11.

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