scholarly journals Detection of insulin-like protein and some active compounds in Bauhinia variegata Linn. leaf ethanolic extracts and the effect in reducing blood glucose levels in mice

2014 ◽  
Vol 8 (2) ◽  
pp. 76-83
Author(s):  
Khaleel Ibrahim Rashid
Keyword(s):  
Blood Glucose ◽  
High Performance ◽  
Ethanolic Extract ◽  
Diabetes In Animals ◽  
Active Compounds ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Ethanolic Extracts ◽  
High Reduction ◽  
Bauhinia Variegata

The study aims to detect the insulin-like protein and determine the active compounds in Bauhinia variegata L. leaves ethanolic extracts that help in reducing the blood glucose levels for white albino mice. The chemical detection of leaves ethanolic extract revealed the presence of tannins, terpenes, steroids, and flavonoids as active compounds. High performance liquid chromatography (HPLC) analysis method, using C18 column resulted in presence of insulin-like protein in the leaves extracts. Results showed significant reduction of blood glucose levels after 3 days of the treatment, and high reduction obtained after 6, 9, 12 days as compared with the human swine insulin used as a control. It was concluded that the crude ethanolic extracts and the partial purified insulin of B. variegata was used to examine the existence of insulin-like protein in this plant. In this study, an attempt has been made to report plant insulin that may be useful to the health professionals and scholars working in the field of pharmacology and therapeutics to develop alternative medicine to cure diabetes in animals and man.

scholarly journals Comparative evaluation of anti-diabetic action and pancreatic histopathology of rats treated with two alkaloidal plant extracts

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1841-1846
Author(s):  
Bonagiri Sreedevi ◽  
Vijaya Kuchana ◽  
Shobharani S
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Bark Extract ◽  
Albino Rats ◽  
Histopathological Study ◽  
Standard Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Soxhlet Apparatus ◽  
Ethanolic Extracts

This study aimed to understand Strychnosnuxvomica and Holarrhena pubescens Stem bark extract action towards M3 receptor in controlling blood glucose levels. Strychnos nux vomica  and Holarrhena pubescens are both alkaloidal drugs can help in controlling Hyperglycemic level. This will be useful in the formulation of a new herbal drug molecule for treating diabetes. Chloroform and ethanolic extracts of selected alkaloidal plants were extracted using the soxhlet apparatus and obtained quotes were tested for acute toxicity studies and carried out anti-diabetic action on Wister albino rats for 21 days. Results obtained from Blood glucose levels and histopathological study of test groups are compared with blood glucose levels of standard group, and highly significant action was identified by the chloroform extract of Strychnos nux vomica and Holarrhena pubescens group. Moderate anti-diabetic action was observed remaining two groups of ethanolic extracts. Strychnos nux vomica and Holarrhena pubescens ethanolic extract groups are acting on M3 receptors and controlling Hyperglycemic levels.

PSXV-16 Blood glucose dynamics after feeding in the Orlov trotter horses in the conditions of the training center in preparation for trotter-racing

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 356-357
Author(s):  
Olga Sharaskina
Keyword(s):  
Blood Glucose ◽  
High Performance ◽  
Free Access ◽  
Training Center ◽  
Factors Affecting ◽  
Intensive Training ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Significant Difference ◽  
Glucose Dynamics

Abstract It is necessary to consider the factors affecting the dynamics and blood glucose (BG) level to maintain a horse’s high performance and health during intensive training. The study aimed to research the influence of the feeding regime on the change in BG level in the Orlov trotter horses during the period of intensive training (summer) in the conditions of the stud farm’s training center in the Kaluga region (Russia). Horses aged 2 to 4 years, stallions (n = 7), and mares (n = 5) received commercial mixed feed three times a day and grass hay in free access. Four times a week after lunch, horses are released into the paddock with cut grass. Blood was collected from the jugular vein. Blood was collected before morning feeding and then every hour until four h after morning and afternoon feeding. The dynamics of BG changes depending on the presence or absence of the grass paddock after feeding were evaluated. If horses remained in the stall after feeding, the BG level gradually increased, reaching a maximum (4.95 ± 0.21 mmol/L) 3 hours after feeding and was significantly higher (P ≤ 0.05) than when they were immediately moved to the paddock with grass. The maximum BG concentration in horses in the paddock was observed 1 hour after feeding (4.55 ± 0.21 mmol/L); it didn’t have significant differences with the BG level after 1 hour in “stall horses” (4.5 ± 0.14 mmol/L). Then the BG level of the “paddock horses” gradually decreased, and in the “stall horses” increased. No significant difference in BG levels was observed 4 hours after feeding. When horses can walk in a paddock after feeding concentrates and eat grass, blood glucose levels do not rise and tend to decrease.

Gynura divaricata Effect on Blood Glucose Levels in Alloxan-Induced Diabetes Mice

2019 ◽  
Vol 13 (01) ◽  
pp. 27-33
Author(s):  
Evi Sovia ◽  
Dian Anggraeny ◽  
Ris Kristiana ◽  
Firhan Hamdi Maulida ◽  
Mutia Susparini
Keyword(s):  
Blood Glucose ◽  
Histological Examination ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Diabetes Mice ◽  
Before And After

Gynura divaricata (GD) also known as Dewa leaf is one of the Indonesian medicinal plants which are also native to India, Thailand and China. GD is widely used as antidiabetic, antihypertensive and other diseases including several tumors. However, research on the effects of GD as antidiabetic is still not widely practiced, especially in Indonesia. The aim of this research is to investigate the effect of GD ethanolic extract on blood glucose levels of alloxan-induced diabetes mice. This research is an experimental research with pre- and post-test design using 24 male DDY strain mice. Animals experiment is divided into four groups, that are one normal group and three alloxan-induced diabetes mice group that give 1% CMC (control), 400[Formula: see text]mg/kg GD ethanolic extract and 0.65[Formula: see text]mg/kg glibenclamide, respectively. Treatment was given for 14 days. Blood glucose levels were measured before and after inducing alloxan, and after 14 days treatment. At the end of study, all of animals experiment were sacrificed for histological examination. Phytochemical analysis revealed that polyphenols, flavonoids, steroids, triterpenoids, terpenoids and and quinones were present in ethanol extract of GD. The results of this study showed significant ([Formula: see text]) decrease of blood glucose levels after GD ethanolic extract and glibenclamide treatment. The results of histological examination showed improvement in pancreas damage and significant increase in the number of beta cells in GD and glibenclamide groups. The results indicate that dewa leaf ethanolic extract has antidiabetic activity and the effectiveness is the same as glibenclamide. GD also could repair damage of pancreas caused by alloxan induction.

scholarly journals Taxithelium napalense acts against free radicals and diabetes mellitus

2017 ◽  
Vol 12 (2) ◽  
pp. 16 ◽  
Author(s):  
Vinay Bharadwaj Tatipamula ◽  
Kishore Naidu Killari ◽  
Vedula Girija Sastry ◽  
Alekhya Ketha
Keyword(s):  
Free Radicals ◽  
Blood Glucose ◽  
Video Clip ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antidiabetic Activity ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Radical Scavenging Properties ◽  
Histopathological Studies

<p class="Abstract">In this work, <em>Taxithelium nepalense</em> ethanolic extract and its fractions were evaluated for the antidiabetic activity in rat based on phytochemical and free radical scavenging properties. The ethanolic extract, fraction IV and V significantly attenuated the blood glucose levels at 600, 200 and 200 mg/kg with 50.0, 33.5 and 42.0% inhibition respectively. The histopathological studies were manifesting the recuperation of damaged cells in liver and pancreas tissues. The outcomes of the present work affirm that the <em>T. nepalense</em> has a potency to plummet the overproduction of free radicals and blood glucose levels in the diabetic-induced rat.</p><p><strong>Video Clip of Methodology</strong>:</p><p>1 min 50 sec   <a href="https://youtube.com/v/GUZp4QY9kCs">Full Screen</a>   <a href="https://youtube.com/watch?v=GUZp4QY9kCs">Alternate</a></p>

In vitro and in vivo anti-diabetic activity of Citrullus colocynthis pulpy flesh with seeds hydro-ethanolic extract

2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Aymen Owais Ghauri ◽  
Saeed Ahmad ◽  
Tayyeba Rehman
Keyword(s):  
Blood Glucose ◽  
Normal Control ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Antioxidant Potential ◽  
Blood Glucose Monitoring ◽  
Glucose Levels ◽  
Blood Glucose Levels

AbstractBackgroundDiabetes is the one of the leading cause of morbidity and mortality. Traditionally phytotherapy is widely being used for diabetes treatment and highly valued. Citrus colocynthis has known anti-diabetic potential. However, anti-diabetic potential of hydro-ethanolic extract of C. colocynthis pulpy flesh with seeds is not reported yet.MethodsThe extract of C. colocynthis pulpy flesh with seeds was done by maceration method using 70% ethanol. To evaluate anti-diabetic and antioxidant potential of the seeded fruit in vitro, α-glucosidase and DPPH inhibition assays was done, respectively. In vivo study used streptozotocin (STZ) induced diabetes model of rats. Rats were randomized in five groups i. e. normal control, negative control, standard control, C. colocynthis 150 and 300 mg/kg. STZ was administered to all groups except normal control. After wards, plant extract and glibenclamide is continued for 14 days. Blood samples were collected from rat tail vein daily and from Cardiac puncture at the end of study. The blood glucose levels were monitored daily by using one-touch blood glucose monitoring system. The blood glucose level was monitored on 0, 1st, 5th, 8th, 11th, and 14th day of induction.ResultsHydro-ethanolic extract of C. colocynthis pulpy flesh with seeds was able to decolorize DPPH and therefore possess antioxidant potential, continuous administration for 14 days showed a marked decrease in serum glucose levels (p 0.01) it is found to be somewhat less effective as glibenclamide (standard control) (p 0.001). A time-dependent decrease in blood glucose levels was observed (351.3 ± 4 to 258 m/kg).ConclusionHydro-ethanolic extract of C. colocynthis pulpy flesh with seeds lowered the serum triglyceride and cholesterol levels in diabetic rats significantly as compared to negative control. The hypoglycemic effect of hydro-ethanolic extract of C. colocynthis pulpy flesh with seeds is may be due to α-glucosidase inhibition potential.

scholarly journals PRELIMINARY PHYTOCHEMISTRY AND ANTIDIABETIC ACTIVITY OF PORTULACA GRANDIFLORA HOOK PLANT EXTRACT ON STREPTOZOTOCIN-INDUCED DIABETES IN RATS

2019 ◽  
pp. 87-90
Author(s):  
DEVI M ◽  
KOMAL S ◽  
LOGESHWARI B
Keyword(s):  
Blood Glucose ◽  
Ethanolic Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Portulaca Grandiflora ◽  
Streptozotocin Induced Diabetes

Objective: The present study was aimed to evaluate the antidiabetic activity of ethanolic extract of the whole aerial plant of Portulaca grandiflora Hook on streptozotocin (STZ)-induced diabetic rats. Methods: Experimental diabetes was induced by a single dose of intraperitoneal injection of STZ (150 mg/kg). Adult male Wister albino rats were divided into five groups; normal control, diabetic control, diabetic glibenclamide (5 mg/kg), diabetic P. grandiflora H. extract (200 mg/kg), and diabetic P. grandiflora H. extract (400 mg/kg) for 21 days and analyzed for body weight (BW) and blood glucose. Results: The STZ-treated diabetic control rats showed a significant increase in blood glucose with a concomitant decrease in BW. Oral administration of P. grandiflora H. extract (200 and 400 mg/kg) and glibenclamide (5 mg/kg) for 21 days showed a significant reduction in blood glucose levels and elevation in the bodyweight studies as compared to control and glibenclamide-treated rats. Conclusion: The results of the present study showed that a potent antidiabetic activity was present in the aerial part of plant P. grandiflora H. extract.

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