Naringin and 5-fluorouracil suppress inflammatory Cytokines in human skin cancer cell line

2021 ◽  
Vol 12 (1) ◽  
pp. 729-733
Author(s):  
Deepa Suruli ◽  
Fathima Bushra Sheriff Mirza ◽  
Gloria Jemmi Christobel R ◽  
Amuthavalli Kottaiswamy ◽  
Shila Samuel ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Cell Line ◽  
Human Skin ◽  
Cell Lines ◽  
Cancer Cell ◽  
A375 Cell ◽  
Ic50 Values ◽  
Anti Inflammatory ◽  
A375 Cells ◽  
Inflammatory Effect

Naringin is a citrus flavonoid recently studied for anti-inflammatory activity in numerous cancer cells. In this study, the anti-inflammatory properties of naringin along with 5-fluorouracil in human skin cancer cell lines A375 was analyzed. A375 cells were treated with naringin, 5-fluorouracil alone, and combination. MTT assay and cell viability assays was demonstrated to detect the inhibitory effects of naringin or 5-fluorouracil on cell proliferation. mRNA expression of TNFα, IL-6, IL-1β, and NFκB were determined using quantitative RT-PCR.  The effect of naringin and 5-fluorouracil combination significantly inhibited the growth and proliferation of the A375 cells in a concentration dependent manner with the IC50 values of naringin (24.75 μM) 5-fluorouracil (2.5 μM). The combination of naringin+5-fluorouracil on A375 cell lines at a concentration of half IC50 values (12µM+1 μM). Naringin and 5-fluorouracil combination also decreased the level of TNFα, IL-6, IL-1β, and NFκB mRNA in the A375 cell line. Naringin and 5-fluorouracil exerted anti-inflammatory effect through the suppression of NF-kB, IL-1β, TNFα, IL-6 in A375 cells. Taken together, our results suggested that treating A375 with naringin and 5-fluorouracil combination may have future applications in treating skin cancers through its anti-inflammatory effect.

CHEMOTHERAPEUTIC POTENTIAL OF NOVEL XANTHONE SOURCED FROM SWERTIA CHIRATA AGAINST SKIN CARCINOGENESIS

2020 ◽  
pp. 84-88
Author(s):  
ATISH BARUA ◽  
PRITHA CHOUDHURY ◽  
CHINMAY KUMAR PANDA ◽  
PROSENJIT SAHA
Keyword(s):  
Skin Cancer ◽  
Antitumor Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Self Renewal ◽  
Qrt Pcr ◽  
Swertia Chirata

Objective: Swertia chirata forms a rich source of bio-active compounds, among which xanthones form an important part. Among the xanthones present in it, 1,5,8 Tri-hydroxy-3-methoxy xanthone (TMX) was found to be the most active. The present study aims to evaluate the chemotherapeutic potential of it against metastatic skin cancer cell lines. Methods: In this study, the antitumor activity of TMX (the active component of chirata plant) was evaluated in A431, SKMEL-5, and A375 cell line by using in-vitro assays such as cell viability assay, cell cycle analysis, caspase 3 activity assay, intracellular reactive oxygen species (ROS) level determination by dichlorofluorescein diacetate, and quantitative real-time polymerase chain reaction (qRT-PCR). Results: In vitro studies showed that TMX from S. chirata exhibited significant antitumor activity by inducing apoptosis and restricting proliferation in both melanoma and non-melanoma skin cancer cell lines, but no such activity was seen in normal skin cancer cell line WS1. The qRT-PCR analysis revealed that in both the melanoma ad non-melanoma cell lines, TMX could exert its antitumor activity by downregulating c-Myc, cyclin-D1, and β-catenin and up-regulating Wnt antagonist gsk-3β, thereby suppressing wnt self-renewal pathway, but such regulation was absent in normal cell line. Conclusions: TMX from chirata could effectively inhibit the proliferation of metastatic skin cancer (both melanoma and non-melanoma) cell lines while being non-toxic to normal cell lines. The chemotherapeutic potential of TMX against metastatic skin cancer cell lines was achieved by downregulating several key regulatory genes enabling the suppression of the self-renewal pathway, the chief reason behind the invasiveness of cancer cells.

scholarly journals Anti-Cancer Acitivity of Etodolac and Its Derivatives on Prostate and Colorectal Cancer Cell Lines

Proceedings ◽  
2018 ◽  
Vol 2 (25) ◽  
pp. 1573
Author(s):  
Sevgi Koçyiğit Sevinç ◽  
Oya Orun ◽  
Pınar Mega Tiber ◽  
Pelin Çıkla-Süzgün ◽  
Ş. Güniz Küçükgüzel
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Prostate Cancer Cell Line ◽  
Inhibition Of Proliferation ◽  
Analgesic Agents ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Apoptotic Effects

Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used as anti-inflammatory and analgesic agents. This family of drugs suppresses prostaglandin synthesis through inhibition of cyclooxygenase (COX) enzymes. Recent studies showed that anti-carcinogenic effects of these drugs are especially mediated by COX-2 enzyme. Etodolac is a COX-2 inhibitor and though not perfectly selective, it exhibits “preferential selectivity” for COX-2. In this study, the anti-proliferative and apoptotic effects of etodolac and its hydrazone or triazole derivatives (SGK 206 and SGK 242, respectively), were investigated on prostate cancer cell line PC-3 and human colorectal carcinoma cell line HT-29. Our data showed that SGK 206 and SGK 242 were more effective in the inhibition of proliferation and induction of apoptosis compared to etodolac in both cell lines.

Anti-inflammatory and Anti-proliferative Properties ofChromolaena odorataLeaf Extracts in Normal and Skin-Cancer Cell Lines

2014 ◽  
Vol 20 (4) ◽  
pp. 359-371 ◽  
Author(s):  
Varma N. G. Yajarla ◽  
Reddy P. Nimmanapalli ◽  
Sridevi Parikapandla ◽  
Geetika Gupta ◽  
Roy Karnati
Keyword(s):  
Skin Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Anti Inflammatory

Activity of mTOR-Inhibitor Rad001 (everolimus) in differentiated and anaplastic thyroid cancer cell lines

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e14608-e14608
Author(s):  
T. Behlendorf ◽  
W. Voigt ◽  
T. Mueller ◽  
K. Jordan ◽  
D. Arnold ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Drug Interaction ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mtor Inhibitor ◽  
Cancer Cell Lines ◽  
Thyroid Cancer Cell ◽  
Ic50 Values ◽  
Thyroid Cancer Cell Lines

e14608 Background: Recent data suggest that aberrant activation of PIK3/AKT-pathway and mTOR are involved in the development of thyroid cancer, particularly of anaplastic (ATC) and follicular (FTC) subtype. Therefore, mTOR could be a potential treatment target in thyroid cancer. Methods: To asses the potential role of mTOR as target for the treatment of thyroid cancer, two human ATC cell lines SW1736 and 8505C, the papillary thyroid cancer (PTC) cell line BCPAP and the FTC cell line FTC133 were exposed for 96h to increasing concentrations of the mTOR inhibitor RAD001 (Everolimus, kindly provided by Novartis, Switzerland). For combination experiments 10 nM of RAD001 were combined with increasing concentrations of either doxorubicin (DOX) or cisplatin (CDDP) continuously. Cytotoxicity was measured using the sulforhodamine B assay. IC50-values were calculated with Sigma Plot (Jandel Scientific) and drug interaction was determined by the model of Drewinko. Results: The observed IC50-values of RAD001 were 1nM (FTC133), 10 nM (BCPAP), 1000 nM (SW1736) and 9400 nM (8505C). In contrast to the pronounced differences in sensitivity as assessed on the basis of IC50, a growth inhibitory effect ≥ 25 % was seen in all cell lines at a concentration of 1 nM of RAD001. IC50 for CDDP ranged from 1,3–4,8 μM and for DOX from 8–40 nM. Combination of 10 nM RAD001 with either DOX or CDDP resulted in additive drug interaction with the exception in cell line 8505C where significant synergy was found for the combination with CDDP. Conclusion: RAD001 exerted interesting preclinical activity in two differentiated thyroid cancer cell lines. Mainly additive drug interaction in thyroid cancer cell lines was observed for combinations with CDDP and DOX. Mechanistic investigations are underway and will be presented at the meeting. At least for differentiated thyroid cancer mTOR-inhibition appeared promising, further evaluation in thyroid cancer seems warranted. No significant financial relationships to disclose.

scholarly journals In-vitro antioxidant, anti-cancer, and anti-inflammatory activities of selected medicinal plants from western Nepal

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Bishnu Prasad Pandey ◽  
Kapil Adhikari ◽  
Suman Prakash Pradhan ◽  
Hee Jeong Shin ◽  
Eun Kyeong Lee ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Antioxidant Properties ◽  
Melia Azedarach ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Flavonoid Content ◽  
Anti Inflammatory

Abstract Background This study was focused on the measurement of anticancer properties of six medicinal plants from western Nepal in three cell lines; HeLa, Hep3B, and HCT116, and anti-inflammatory properties in RAW 264.7 cell line through NO, PGE2, and TNF-α production. In addition, the phytochemical screening, total phenolic, flavonoid content, and antioxidant properties were evaluated. Results The qualitative phytochemical analysis revealed the presence of different secondary metabolite and range of total phenolic and total flavonoid content. The highest antioxidant activities were observed in Bergenia pacumbis against both DPPH (IC50 = 25.97 ± 0.19 μg/mL) and ABTS (IC50 = 14.49 ± 0.40 μg/mL). Furthermore, the highest antiproliferative effect against cervical, liver, and colon cancer cell lines were observed in Melia azedarach as IC50 values of 10.50, 5.30, and 1.57 μg/mL respectively, while the strongest anti-metastatic potential on liver cancer cell line was found in Pleurospermum benthamii. In addition, P. benthamii showed the most potent anti-inflammatory effect in RAW264.7 murine macrophage cells. Conclusion This study provided the evidence for M. azedarach and P. benthamii to have great anticancer potential and finding builds the enough scientific backgrounds in future to isolate and purify the bioactive compounds for further applications.

scholarly journals The Oncolytic Effect of Respiratory Syncytial Virus (RSV) in Human Skin Cancer Cell Line, A431

2013 ◽  
Vol 15 (1) ◽  
pp. 62-7 ◽  
Author(s):  
Vahid Salimi ◽  
Masoumeh Tavakoli Yaraki ◽  
Mahmood Mahmoodi ◽  
Shahram Shahabi ◽  
Mohammad Javad Gharagozlou ◽  
...  
Keyword(s):  
Respiratory Syncytial Virus ◽  
Skin Cancer ◽  
Cell Line ◽  
Human Skin ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
Syncytial Virus ◽  
Oncolytic Effect

A green approach toward quinoxalines andbis-quinoxalines and their biological evaluation against A431, human skin cancer cell lines

2013 ◽  
Vol 5 (12) ◽  
pp. 1377-1390 ◽  
Author(s):  
Debasish Bandyopadhyay ◽  
Jessica Cruz ◽  
Liza D Morales ◽  
Hadi D Arman ◽  
Erica Cuate ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Human Skin ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Biological Evaluation ◽  
Green Approach

scholarly journals Ethanolic extract of Nymphaea lotus L. (Nymphaeaceae) leaves exhibits in vitro antioxidant, in vivo anti-inflammatory and cytotoxic activities on Jurkat and MCF-7 cancer cell lines

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Benoit Banga N’guessan ◽  
Adwoa Dede Asiamah ◽  
Nana Kwame Arthur ◽  
Samuel Frimpong-Manso ◽  
Patrick Amoateng ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ethanolic Extract ◽  
Cancer Cell Lines ◽  
Paw Oedema ◽  
Anti Inflammatory ◽  
Chang Liver ◽  
Mcf 7

Abstract Background Nymphaea lotus L. (N. lotus) is an aquatic plant with anecdotal reports suggesting its use in the traditional management of cancer. However, there is a paucity of data on the antioxidant, anti-inflammatory and cytotoxic properties of N. lotus in relation to its phytochemical and elemental contents. This study aimed at determining the antioxidant, anti-inflammatory and cytotoxic properties of the hydro-ethanolic extract of N. lotus leaves (NLE), and its phenolic, flavonoid and elemental constituents. Methods The antioxidant property of NLE was determined using total phenolic and flavonoid, DPPH radical scavenging, lipid peroxidation and reducing power assays. The anti-inflammatory activity of NLE (100–250-500 mg/kg), diclofenac and hydrocortisone (positive controls) were determined by paw oedema and skin prick tests in Sprague Dawley rats. Also, the erythrocyte sedimentation rate (ESR) was determined by Westergren method. The macro/micro-elements content was determined by the XRF method. The cytotoxic property of NLE was determined by the MTT assay, on two cancer cell lines (MCF-7 and Jurkat) and compared to a normal cell line (Chang liver). Inhibitory concentrations were determined as IC50 values (±SEM). Results The extract had appreciable levels of phenolic and flavonoids compounds and was two-fold more potent in scavenging DPPH radicals than Butylated hydroxytoluene (BHT). However, NLE was three- and six-fold less potent than ascorbic acid and BHT, respectively, in reducing Fe3+ to Fe2+. The extract was six-fold more potent than gallic acid in inhibiting lipid peroxidation. The extract caused a dose-dependent decrease in rat paw oedema sizes, comparable to diclofenac, and a significant decrease in wheel diameters and ESR. The elemental analysis revealed relevant concentrations of Mg2+, P2+, S2+, K2+, Mn+, Fe+, Cu+, Zn+ and Cd+. The extract exhibited cytotoxic activity on both MCF-7 (IC50 = 155.00 μg/ml) and Jurkat (IC50 = 87.29 μg/ml), with higher selectivity for Jurkat cell line. Interestingly, the extract showed low cytotoxicity to the normal Chang liver cell line (IC50 = 204.20 μg/ml). Conclusion N. lotus leaves extract exhibited high antioxidant, anti-inflammatory and cancer-cell-specific cytotoxic properties. These aforementioned activities could be attributed to its phenolic, flavonoid and elemental constituents. Graphical abstract

scholarly journals Polyacetylenes from the Roots of Swietenia macrophylla King

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1291 ◽  
Author(s):  
Cheng-Neng Mi ◽  
Hao Wang ◽  
Hui-Qin Chen ◽  
Cai-Hong Cai ◽  
Shao-Peng Li ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Cancer Cell ◽  
Carcinoma Cell ◽  
Leukemia Cell ◽  
Cancer Cell Lines ◽  
Carcinoma Cell Line ◽  
Leukemia Cell Line ◽  
Swietenia Macrophylla ◽  
Ic50 Values

A phytochemical investigation of the roots of Swietenia macrophylla led to the isolation of seven polyacetylenes, including five new compounds (15) and two known ones (67). Their structures were elucidated by extensive spectroscopic analysis and detailed comparison with reported data. All the isolates were tested for their cytotoxicity against the human hepatocellular carcinoma cell line BEL-7402, human myeloid leukemia cell line K562, and human gastric carcinoma cell line SGC-7901. Compounds 1 and 6 showed moderate cytotoxicity against the above three human cancer cell lines with IC50 values ranging from 14.3 to 45.4 μM. Compound 4 displayed cytotoxicity against the K562 and SGC-7901 cancer cell lines with IC50 values of 26.2 ± 0.4 and 21.9 ± 0.3 μM, respectively.

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