scholarly journals The influence of habitat on metabolic and digestive parameters in an intertidal crab from a SW Atlantic coastal lagoon

2021 ◽  
Vol 151 ◽  
Author(s):  
Eugenia Méndez ◽  
M. Soledad Michiels ◽  
Alejandra A. López-Mañanes
Keyword(s):  
Lipase Activity ◽  
Coastal Lagoon ◽  
Protein Concentration ◽  
Metabolic Parameters ◽  
Adult Males ◽  
Experimental Diet ◽  
Neohelice Granulata ◽  
Intertidal Crab ◽  
In The Wild ◽  
Sw Atlantic

The hepatopancreas of decapod crustaceans is an organ which can act as indicator for digestive and metabolic parameters under different physiological and / or environmental conditions. However, biochemical studies on digestive and metabolic parameters of the hepatopancreas of euryhaline burrowing crabs such as Neohelice granulata from habitats with different diet compositions are still scarce. In the wild, adult males of N. granulata from Mar Chiquita Coastal Lagoon (Argentina) in mudflat habitat have diets with higher lipid and protein content than crabs from the saltmarsh, suggesting that diets could be an important factor influencing hepatopancreas activities. We tested this hypothesis here by exposing adult male crabs to a similar experimental diet and comparing hepatopancreas parameters for lipid components and protein metabolism between males from these two habitat types at different times (up to three months). At month 3, we noticed a decrease of the triglyceride concentration and lipase activity and an increase of protein concentration in crabs from the mudflat. In contrast, triglycerides and protein concentration did not change in crabs from the saltmarsh, while lipase activity decreased and levamisole insensitive AP increased at month 3. The results indicate that digestive and metabolic parameters in the hepatopancreas of crabs from habitats varying in diet content remain different, even if crabs are subsequently fed by a similar experimental diet. This suggests that specific intrinsic regulations of these hepatopancreas parameters could operate differently in each habitat and could not be changed by recent diet conditions.

scholarly journals Hatching and larval export of the intertidal crab Neohelice granulata in Mar Chiquita coastal lagoon, Argentina

2013 ◽  
Vol 103 (2) ◽  
pp. 124-133 ◽  
Author(s):  
Guillermina Sánchez Vuichard ◽  
Nahuel Farías ◽  
Tomás Luppi
Keyword(s):  
Embryonic Development ◽  
Coastal Lagoon ◽  
Time Scale ◽  
Tidal Range ◽  
Salinity Range ◽  
Dark Cycle ◽  
Endogenous Rhythms ◽  
Neohelice Granulata ◽  
Intertidal Crab ◽  
Larval Hatching

Synchronization in the events of the reproductive cycle in female Neohelice granulata Dana, 1851 were studied from samples taken weekly and biweekly from September to December 2006 in the Laguna Mar Chiquita. The timing and larval hatching and synchronicity were inferred from numbers of ovigerous females and observing the stages of embryonic development. Synchronization in larval hatching also was observed in females in experiments in dark for a period of 48 hours, at three different salinities (10, 23 and 33 ppm). In addition plankton sampling were performed in order to study larval exportation at the field and its link to the tidal and light/dark cycles. We found that ovigerous females of N. granulata have a marked synchronization in embryonic development which results in that most of berried females are close to hatching within a period of maximum tidal range (days). Within this period, there is a synchronization of hatching at a time scale of hours, governed by environmental conditions. The salinity range used in this study (10-32‰) did not affect hatching synchronicity neither time to hatch. Hatching was synchronized according to endogenous rhythms governed mainly by the tidal cycle and secondarily by the breadth of it. It is also conditioned by the light-dark cycle through an exogenous cycle, so that the hatchings would occur mostly at night high tides.

Digestive flexibility in a euryhaline crab from a SW Atlantic coastal lagoon: alkaline phosphatase activity sensitive to salinity in the hepatopancreas

2015 ◽  
Vol 95 (6) ◽  
pp. 1133-1140 ◽  
Author(s):  
Silvina Andrea Pinoni ◽  
Eugenia Méndez ◽  
Alejandra Antonia López Mañanes
Keyword(s):  
Alkaline Phosphatase ◽  
Coastal Lagoon ◽  
Buenos Aires Province ◽  
Neohelice Granulata ◽  
Low Salinity ◽  
Sw Atlantic ◽  
Short And Long Term ◽  
Biochemical Level

We studied biochemical characteristics and the response to low salinity at short and long-term after feeding of alkaline phosphatase (AP) activity in hepatopancreas of the osmoregulator crab Neohelice granulata from Mar Chiquita coastal lagoon (Buenos Aires Province, Argentina) (37°32′–37°45′S 57°19′–57°26′W). The hepatopancreas exhibited a levamisole-insensitive and a levamisole-sensitive AP activity with distinct characteristics. Levamisole-insensitive activity was similar within the range of pH 7.4–9.0 and exhibited a Michaelis–Menten kinetics. Levamisole-sensitive AP activity appeared to be maximal at pH 8.5 and appeared to exhibit an allosteric kinetics. In crabs acclimated to 10 psu (hyper-regulation conditions) levamisole-insensitive and levamisole-sensitive AP activity increased (about 16-fold) over time from short term (2–4 h) to long term (120 h) after feeding while no changes occurred in crabs acclimated to 35 psu (osmoconforming conditions). The changes of AP activity along with the higher values at 120 h after feeding in 10 psu compared with those in 35 psu, and the concomitant changes in proteolytic activity, suggest a role of AP in digestive and metabolic adjustments at the biochemical level upon hyper-regulatory conditions.

Effects of a High Cholesterol–Oil Diet for Rabbits on Concentrations of Heparin and Total Sulfated Mucopolysaccharide in Aorta and Other Tissues Measured by Microelectrophoresis

1974 ◽  
Vol 52 (3) ◽  
pp. 661-668 ◽  
Author(s):  
T. K. Sue ◽  
L. B. Jaques
Keyword(s):  
Lipoprotein Lipase ◽  
Large Intestine ◽  
Serum Cholesterol ◽  
Lipase Activity ◽  
Serum Levels ◽  
Lipoprotein Lipase Activity ◽  
High Cholesterol ◽  
Experimental Diet ◽  
Small And Large Intestine

Rabbits were fed a high cholesterol–oil diet for 13 weeks. The amounts of total sulfated mucopolysaccharides and heparin in aorta, heart, skin, and small and large intestine were determined by microelectrophoresis. Aortic lipoprotein lipase activity, serum cholesterol, and triglycerides were also measured. The results showed that quantities of total sulfated mucopolysaccharides, heparin, and lipoprotein lipase activity increased markedly in aortas but were without change in other tissues in the rabbits on the experimental diet. There was a high correlation between lipoprotein lipase activity and concentrations of both heparin and total sulfated mucopolysaccharides in aorta, and also between concentrations of heparin and total sulfated mucopolysaccharides in aorta and serum levels of triglyceride and cholesterol.

scholarly journals Learning a non-neutral conditioned stimulus: place preference in the crab Neohelice granulata

2020 ◽  
Author(s):  
Martín Klappenbach ◽  
Candela Medina ◽  
Ramiro Freudenthal
Keyword(s):  
Conditioned Stimulus ◽  
Food Pellet ◽  
Place Preference ◽  
Food Sources ◽  
Long Term Memory ◽  
Target Area ◽  
Neohelice Granulata ◽  
Preference Task ◽  
In The Wild ◽  
First Time

AbstractIn the wild, being able to recognize and remember specific locations related to food sources and the associated attributes of landmarks is a cognitive trait important for survival. In the present work we show that the crab Neohelice granulata can be trained to associate a specific environment with an appetitive reward in a conditioned place preference task. After a single training trial, when the crabs were presented with a food pellet in the target quadrant of the training arena, they were able to form a long-term memory related to the event. This memory was evident at least 24 h after training and was protein-synthesis dependent. Importantly, the target area of the arena proved to be a non-neutral environment, given that animals initially avoided the target quadrant. In the present work we introduce for the first time an associative one-trial memory paradigm including a conditioned stimulus with a clear valence performed in a crustacean.

Observations on Activity Patterns, Population and Den Characteristics of The Water Rat Hydromys chrysogaster Along Badger Creek, Victoria.

1995 ◽  
Vol 18 (1) ◽  
pp. 71
Author(s):  
J.L. Gardner ◽  
M. Serena
Keyword(s):  
Population Density ◽  
Activity Patterns ◽  
Home Ranges ◽  
Adult Males ◽  
Fish Farms ◽  
Age Classes ◽  
Irrigation Channels ◽  
In Captivity ◽  
In The Wild ◽  
Hydromys Chrysogaster

The Water Rat Hydromys chrysogaster is Australia's largest amphibious rodent, occupying freshwater rivers, lakes, and coastal and estuarine habitats throughout the continent (Watts and Aslin 1981). Little is known of the species' social organisation or use of space in the wild although Harris (1978) suggested that adults might be intrasexually aggressive. The home ranges of all sex and age classes overlap to some extent but home ranges of adults of the same sex appear to overlap less (Harris 1978). Adult males occupy the largest home ranges which overlap those of one or more females. In captivity individuals kept in groups form hierarchies in which only the dominant females usually breed successfully (Olsen 1982). Fighting occurs primarily among males, with the highest incidence of injuries observed at the beginning of the main September-March breeding season (Olsen 1980, 1982). The results of trapping studies indicate that population density may vary considerably, with the greatest numbers of animals typically occupying man-modified habitats such as irrigation channels or fish farms (McNally 1960, Watts and Aslin 1981, Smales 1984). Aggressive behaviour appears to be related to pelage colour (phenotype) and population density; the higher the density the greater the number of injured individuals (Olsen 1980).

Waterbird Response to Changes in Habitat Area and Diversity Generated by Rainfall in a SW Atlantic Coastal Lagoon

Waterbirds ◽  
2007 ◽  
Vol 30 (4) ◽  
pp. 541-553 ◽  
Author(s):  
Alejandro D. Canepuccia ◽  
Juan P. Isacch ◽  
Domingo A. Gagliardini ◽  
Alicia H. Escalante ◽  
Oscar O. Iribarne
Keyword(s):  
Coastal Lagoon ◽  
Habitat Area ◽  
Sw Atlantic ◽  
Atlantic Coastal

scholarly journals Reproductive Behaviors of Wild Chinese Pangolin (Manis Pentadactyla): A Case Study Based on Long-Term Monitoring

2021 ◽  
Author(s):  
Nick Ching-Min Sun ◽  
Kurtis Jai-Chyi Pei ◽  
Li-Yue Wu
Keyword(s):  
Maternal Separation ◽  
Post Partum ◽  
Activity Patterns ◽  
Camera Traps ◽  
Adult Males ◽  
Reproductive Behaviors ◽  
In The Wild ◽  
Manis Pentadactyla ◽  
History Of ◽  
Almost All

Abstract Observations of Chinese pangolin (Manis pentadactyla) in the wild are extremely rare and challenging because of their nocturnal and cryptic activity patterns and low population density. The present article reported the first field observation in eastern Taiwan, from October 4, 2012 to June 16, 2016, on the reproductive behavior of the Chinese pangolin based on the monitoring of a female (LF28) using radiotelemetry and camera traps. During this period, LF28 aged from 1 to 4.5-years old and gave two single-births, both took place in early December, at 3 and 4 years old, respectively. We recorded the entire 157 days of the first nursing period from parturition to maternal separation. For the second infant, the gestation period was estimated to be around 150 days based on the evidence that the pregnancy started in early Jul. 2015 and the offspring was born on Dec. 9, 2015. During the entire nursing period, LF28 frequently moved the offspring from one nursing burrow to another staying various durations ranging from 1 day to more than 35 days, and almost all (= 15/16) of these burrows were located in the core (MCP75) of LF28’s home range. Started from the month of parturition and lasting throughout the whole nursing period, different adult males constantly visiting the nursing burrows were recorded. Mating behavior was recorded once outside the burrow in March, which provided evidence of the occurrence of post-partum estrus in this species. Delay implantation was proposed based on the observation of a several months lag between copulation and the estimated pregnancy initiation date. The present study demonstrated the advantage of using remote technologies to learn the life history of resting fossorial species.

scholarly journals Determinants of differences in the activity budgets of Rhinopithecus bieti by age/sex class at Xiangguqing in the Baimaxueshan nature reserve, China

2015 ◽  
Vol 67 (2) ◽  
pp. 675-683 ◽  
Author(s):  
Yanhong Li ◽  
Dayong Li ◽  
Baoping Ren ◽  
Jie Hu ◽  
Baoguo Li ◽  
...  
Keyword(s):  
Nature Reserve ◽  
Reproductive Investment ◽  
Ecological Factors ◽  
Developmental Differences ◽  
Adult Males ◽  
Activity Budgets ◽  
Rhinopithecus Bieti ◽  
Adult Females ◽  
Class 1 ◽  
In The Wild

Ecological factors are known to influence the activity budgets of Yunnan snub-nosed monkeys (Rhinopithecus bieti). However, little is known about how activity budgets vary between age/sex classes, because the species is difficult to observe in the wild. This study provides the first detailed activity budgets subdivided by age/sex classes based on observations of the largest habituated group at Xiangguqing in Baimaxueshan Nature Reserve. This study was conducted from June 2008 to May 2009. We found that adult females spent more time feeding (44.8%) than adult males (39.5%), juveniles (39.1%), and infants (14.2%). Adult males allocated more time to miscellaneous activities (12.5%) than did adult females (3.8%). Infants were being groomed 6.9% of the time, which was the highest proportion among all age/sex classes. Adults spent more time feeding, while immature individuals allocated more time to moving and other activities. There are several reasons activity budgets may vary by age/sex class: 1) differential reproductive investment between males and females; 2) developmental differences among the age categories; 3) social relationships between members of different age/sex classes, particularly dominance. In addition, group size and adult sex ratio may also impact activity budgets. These variations in activity budgets among the different age/sex classes may become a selective pressure that shapes the development and growth pattern in this species. <br><br><font color="red"><b> This article has been retracted. Link to the retraction <u><a href="http://dx.doi.org/10.2298/ABS160112009E">10.2298/ABS160112009E</a><u></b></font>

scholarly journals Management of Achatina fulica (Bowdich, 1822) (Pulmonata: Achatinidae) in lettuce (Lactuca sativa L.)

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Lindinalva Santos ◽  
Carla Barbosa Negrisoli ◽  
Maciel Bispo Santos ◽  
Aldomario Negrisoli Junior
Keyword(s):  
Enzymatic Activity ◽  
Lipase Activity ◽  
Protein Concentration ◽  
Lethal Dose ◽  
Alcoholic Extract ◽  
Achatina Fulica ◽  
Before And After ◽  
Important Pest ◽  
Lethal Time

ABSTRACT: The giant African snail Achatina fulica was introduced in Brazil and since then has become an important pest, because of its resistance to abiotic conditions, hermaphroditism, polyphagia, and absence of natural predators. This study aims to evaluate the control of A. fulica in lettuce, in Alagoas, Brazil. Bioassays for the determination of lethal dose and lethal time to adults of A. fulica and the egg mortality were performed in the laboratory by applying commercial synthetic products, commercial and non-commercial alcoholic botanical extracts on mollusk adults. Additionally, the protein concentration, lipase activity and enzyme acetylcholinesterase (AChE), and butyrylcholinesterase (BuChE), in the stomach, intestine, nervous ganglion and liver were determined. The alcoholic extract of Capsicum frutescens caused higher mortality of A. fulica, and the alcoholic extract of C. frutescens and Piper tuberculatum oil can prevent the hatching of A. fulica. The lipase activity was present and in greater quantities in tissues, stomach, intestine, liver and ganglia of A. fulica, before and after exposure of the alcoholic extract of C. frutescens. The enzymatic activity of BuChE was present in the ganglia and liver of A. fulica, prior to exposure of the alcoholic extract of C. frutescens. The enzymatic activity of AChE was present only in the ganglion and absent in liver of A. fulica, prior to exposure of the alcoholic extract of C. frutescens. The concentration of 10% of the alcoholic extract of C. frutescens caused 84% mortality of adult A. fulica in lettuce in field conditions.

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