Phenolic Content and Antibacterial Activity of Cupressus sempervirens Leaves Extracts Against Phytopathogenic Bacteria Pectobacterium atrosepticum Causative Agent of Soft Rot on Potato and Clinical Bacteria Strains

AbstractBacterial metal reducers were isolated from water samples collected from harsh condition locations in Egypt. Four selected isolates were identified as Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculata for Copper (Cu), Iron (Fe), Cobalt (Co) and Zinc (Zn) Nanoparticles (NPs) production sequentially. Nitrate reductase enzyme was assayed for bacterial isolates which demonstrated that P. putida, and M. hydrocarbonoclasticus have the maximum enzyme production. The produced NPs were characterized by using XRD, TEM, UV–VIS spectroscopy. Magnetic properties for all selected metals NPs were measured using Vibrating Sample Magnetometer (VSM) and demonstrated that FeNPs recorded the highest magnetization value. The antibacterial activity of selected metals NPs was tested against some phytopathogenic bacteria causing the following diseases: soft rot (Pectobacterium carotovorum, Enterobacter cloacae), blackleg (Pectobacterium atrosepticum and Dickeya solani), brown rot (Ralstonia solanacearum), fire blight (Erwinia amylovora) and crown gall (Agrobacterium tumefaciens). All metals NPs showed an antagonistic effect against the tested isolates, particularly, FeNPs showed the highest antibacterial activity followed by CuNPs, and ZnNPs. Due to the small size, high reactivity, and large surface area of biologically synthesized NPs, they are used as a good disinfector, and can be considered as a new and alternative approach to traditional disease management methods.

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Transformation of Inferior Tomato into Preservative: Fermentation by Multi-Bacteriocin Producing Lactobacillus paracasei WX322

Foods ◽

10.3390/foods10061278 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1278

Author(s):

Rong Zhu ◽

Xiaoqing Liu ◽

Xiaofen Li ◽

Kaifang Zeng ◽

Lanhua Yi

Keyword(s):

Antibacterial Activity ◽

Value Added ◽

Soft Rot ◽

Bacteriocin Production ◽

Lactobacillus Paracasei ◽

Tomato Juice ◽

Vegetable Waste ◽

The Third ◽

Innovative Method ◽

Great Damage

Loss and waste of postharvest vegetables are the main challenges facing the world’s vegetable supply. In this study, an innovative method of value-added transformation was provided: production of bacteriocin from vegetable waste, and then its application to preservation of vegetables. Antibacterial activity to soft rot pathogen Pectobacterium cartovorum (Pcb BZA12) indicated that tomato performed best in the nutrition supply for bacteriocin production among 12 tested vegetables. Moreover, the antibacterial activity was from Lactobacillus paracasei WX322, not components of vegetables. During a fermentation period of 10 days in tomato juice, L. paracasei WX322 grew well and antibacterial activity reached the maximum on the tenth day. Thermostability and proteinase sensitivity of the bacteriocin from tomato juice were the same with that from Man-Rogosa-Sharpe broth. Scanning electron microscope images indicated that the bacteriocin from tomato juice caused great damage to Pcb BZA12. At the same time, the bacteriocin from tomato juice significantly reduced the rotten rate of Chinese cabbage from 100% ± 0% to 20% ± 8.16% on the third day during storage. The rotten rate decrease of cucumber, tomato, and green bean was 100% ± 0% to 0% ± 0%, 70% ± 14.14% to 13.33% ± 9.43%, and 76.67% ± 4.71% to 26.67% ± 4.71%, respectively. Bacteriocin treatment did not reduce the rotten rate of balsam pear, but alleviated its symptoms.

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Isolation, Chemical Composition, Physicochemical Properties, and Antibacterial Activity of Cupressus sempervirens L. Essential Oil

Journal of Essential Oil Bearing Plants ◽

10.1080/0972060x.2021.1924083 ◽

2021 ◽

pp. 1-14

Author(s):

Hayfa Argui ◽

Oumayma Ben Youchret-Zalleza ◽

Salih Can Suner ◽

Çagdas Deniz Periz ◽

Gülen Türker ◽

...

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Physicochemical Properties ◽

Cupressus Sempervirens

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Identification of Secondary Metabolites and Antibacterial Activity of Non Polar Fraction from Heterotrigona itama Propolis

Journal of Fundamental and Applied Pharmaceutical Science ◽

10.18196/jfaps.v2i1.12406 ◽

2021 ◽

Vol 2 (1) ◽

pp. 23-33

Author(s):

Abdul Aziz ◽

Veggy Nadya Yuliawan ◽

Paula Mariana Kustiawan

Keyword(s):

Antibacterial Activity ◽

Methanol Extract ◽

Phenolic Content ◽

Polar Fraction ◽

Hexane Fraction ◽

Diffusion Method ◽

Total Phenolic ◽

Phytochemical Analysis ◽

Phytochemical Content ◽

Color Changes

Propolis is one of the natural products produced by kelulut bees and is still not widely used. The type of stingless bee that is the prima donna in the community is Heterotrigona itama. This study aims to determine the phytochemical content of the n-hexane fraction of Heterotrigona itama bee propolis collected from Kutai Kartanegara, East Kalimantan. The n-hexane fraction was obtained from the methanol extract of H. itama propolis by the liquid-liquid partition method. After obtaining the n-hexane fraction, the research continued with a qualitative phytochemical test to identify the compound and determine total phenolic. Antibacterial activity was determined by the agar well diffusion method with a serial concentration in Escherichia coli bacteria. Qualitative phytochemical analysis in the form of color changes showed that the n-hexane fraction of H. itama propolis contained flavonoids, alkaloids, saponins, and tannins. Based on the results, the total phenolic content of the n-hexane fraction sample was 490 mgGAE/100 g. It caused the n-hexane fraction to have lower phenolic content than the methanol extract (792 mg GAE100 g). Furthermore, this result indicated that the non-polar fraction was not substantial enough to extracted phenolic compounds. It correlated to the antibacterial activity of the n-hexane fraction, which was very weak (2 mm ± 1.5) at 200µg/mL concentration.

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Co-occurrence of latent Dickeya and Pectobacterium species in potato seed tuber samples from northern Finland

Agricultural and Food Science ◽

10.23986/afsci.101446 ◽

2021 ◽

Vol 30 (1) ◽

Author(s):

Yeshitila Degefu

Keyword(s):

Seed Tuber ◽

Single Species ◽

Soft Rot ◽

Potato Seed ◽

Pcr Assay ◽

Diagnostic Pcr ◽

Pectobacterium Atrosepticum ◽

Dickeya Solani ◽

Different Strains ◽

Potato Seed Tuber

Recent methodological developments have uncovered the etiological diversity of the potato blackleg and soft rot Pectobacteriaceae. At least five species in the genera Dickeya and Pectobacterium have been confirmed to cause blackleg on potatoes in Finland. The bacteria are seed borne and remain latent in the tuber until conditions favourable for growth, multiplication and infection prevail. Tubers could be infected by one or more of these species. This short communication is based on the results of molecular detection data collected for more than 14 years from potato seed lots produced in Finland. Diagnostic PCR assay specific to Dickeya solani, Pectobacterium atrosepticum, Pectobacterium carotovorum, P. brasiliense and P. parmentieri revealed that potatoes are infected by one or more of these species; it also revealed that single species infection is more common than multiple colonization. An event of simultaneous occurrences of different strains from the Pectobacterium species appears to be more frequent than that observed between Dickeya and Pectobacterium species. The absence of co-occurrence of Dickeya solani and Pectobacterium atrosepticum is intriguing.

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Effect of physicochemical properties and phenolic compounds of bifloral propolis on antioxidant and antimicrobial capacity

Nova Scientia ◽

10.21640/ns.v12i24.2134 ◽

2020 ◽

Vol 12 (24) ◽

Author(s):

Rey David Vargas Sánchez ◽

Evelin Martínez Benavidez ◽

Javier Hernández ◽

Gastón Ramón Torrescano Urrutia ◽

Armida Sánchez Escalante

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Phenolic Compounds ◽

Physicochemical Properties ◽

Phenolic Content ◽

Biological Activities ◽

Sensory Characteristics ◽

Total Phenolic ◽

Gram Positive ◽

Phenolic Components

In this study the effect of pollen source (mesquite and catclaw) on the sensory characteristics (appearance, color, aroma, taste, consistency and visible impurities), and physicochemical properties of raw propolis, and the phenolic content and biological activities of propolis extracts (PEs) was determined. The phenolic composition of PEs was determined by the total phenolic (TPC), flavone and flavonol (FFC), and flavanone and dihydroflavonol content (FDC). The individual phenolic components were analyzed by HPLC-DAD. The antioxidant activity was determined by the ferric-reducing power (FRAP) and free-radical scavenging activity (FRS). The antibacterial activity was evaluated against Gram-positive (Staphylococcus aureus and Listeria innocua) and Gram-negative (Echerichia coli and Salmonella thyphimurium) bacteria. The results showed that sensory characteristic and physicochemical properties of mesquite and catclaw propolis complied with international quality regulations. Fifteen phenolic compounds were identified, of which pinocembrin, naringenin, galangin, chrysin and quercetin were found a high concentration (> 3 mg/g). Mesquite propolis had the highest phenolic content (TFC and FDC), as well as antioxidant activity (> 2.5 mg Fe (II) equivalent/g; > 40% of DPPH radical inhibition) and antibacterial activity against Gram-positive bacterias in the order S. aureus > L. innocua (> 50% of inhibition for both bacterias at 500 µg/mL). These results indicating that pollen source affect the sensory characteristics and physicochemical properties of propolis, as well as the biological activity of their extracts.

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Effects of Culture Conditions on the Antibacterial Activity of Streptomyces Spp. against Erwinia Spp. Causing Soft Rot Disease on Asparagus Officinalis

Vietnam Journal of Agricultural Sciences ◽

10.31817/vjas.2021.4.2.08 ◽

2021 ◽

Vol 4 (2) ◽

pp. 1077-1086

Author(s):

Nguyen Thanh Trung ◽

Nguyen Thi Van Anh ◽

Tran Thi Dao ◽

Nguyen Thanh Huyen ◽

Pham Le Anh Minh ◽

...

Keyword(s):

Antibacterial Activity ◽

Ornamental Plants ◽

Soft Rot ◽

Antibacterial Activities ◽

Culture Conditions ◽

Asparagus Officinalis ◽

Effective Control ◽

Soft Rot Disease ◽

Streptomyces Spp ◽

Rot Disease

Erwinia is a genus of Enterobacteriacea containing mostly pathogens, which cause soft rot disease in many ornamental plants and crops, including Asparagus officinalis. Chemical treatments to control Erwinia have lost their attractiveness because of the development of resistant strains and the negative impacts on the environment and human health. Therefore, the study of biological controls of soft rot disease has gained great importance. There are several types of microorganisms that show activity against Erwinia spp. such as Pseudomonas fluorescence, Bacillus subtilis, and Streptomyces spp. Among them, Streptomyces spp. are found to be the most effective control agents. In this study, 64 isolates of Streptomyces were screened for their antibacterial activity against Erwinia spp. The results indicated that 18 isolates showed an antagonistic reaction against Erwinia spp. Among them, isolate D5.1 showed the highest inhibition activity. In addition, the morphological and antibacterial activities of isolate D5.1 grown in different conditions were also characterized.

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