scholarly journals Effectiveness of Herbal Extract (Piper retrofractum, Curcuma aeruginosa, and Curcuma zanthorrhiza) as Immunomodulator in Non-Specific Immunity System of Tiger Grouper (Epinephelus fuscoguttatus) against Infection from Vibrio alginolyticus and Vibrio parah

2019 ◽  
Vol 4 (4) ◽  
pp. 94
Author(s):  
Wilis Setyati ◽  
Subagiyo Subagiyo ◽  
Rini Pramesti ◽  
Delianis Pringgenies
Keyword(s):  
Challenge Test ◽  
Vibrio Alginolyticus ◽  
Total Leukocyte Count ◽  
Specific Immunity ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Piper Retrofractum ◽  
Phagocytosis Activity ◽  
Herb Extract ◽  
Pathogenic Infection

The success of aquaculture is one of the supporting factors in food sustainability, one of such is the aquaculture of tiger grouper (Epinephelus fuscoguttatus). The main issue of tiger grouper (Epinephelus fuscoguttatus) aquaculture is disease infestation caused by viruses, microbes, and fungi (1). One of the technologies to control such infestation is the immunostimulant of non-specific immunity. Traditional herbs such as Piper retrofractum, Curcuma aeruginosa, and Curcuma zanthorrhiza have been proven to enhance the immunity system. This study aims to determine the optimal dosage of herb extract (Piper retrofractum, Curcuma aeruginosa, and Curcuma zanthorrhiza) to strengthen non-specific immunity of tiger grouper (Epinephelus fuscoguttatus), to record data on total leukocyte count and phagocytosis activity in the subject against pathogenic infection from Vibrio alginolyticus and Vibrio parahaemolyticus, and on the side effects of  the application of the herbal extracts on meat quality (organoleptic) of tiger grouper (Epinephelus fuscoguttatus)(2). The study was carried out by experimental method using random design with 3 repetitions. The experiment was performed in plastic tanks using flow through system with siphon construction. Steps performed include: Herb sample collection, herb samples extraction, herb extract supplementation into artificial food, dose optimization of herb extract application, challenge test, and side effect test (3). The result shows that treatment with 1% Piper retrofractum, 0.5% Curcuma aeruginosa, and 1% Curcuma zanthorrhiza herb extract increased total leukocyte count and phagocytosis activity in tiger grouper (Epinephelus fuscoguttatus). Organoleptic test of all three treatments indicated similar results, with solid texture and bland flavor. Challenge test against pathogenic infection from V. alginolyticus and V. parahaemolyticus found that the treatment reduced the mortality rate to 0%. Based on the results of this study, Piper retrofractum, Curcuma aeruginosa, and Curcuma zanthorrhiza were proven to be viable immunomodulator on non-specific immunity system in tiger grouper (Epinephelus fuscoguttatus) fish against infection from Vibrio alginolyticus and Vibrio parahaemolyticus.

scholarly journals ANALISIS KESESUAIAN PERAIRAN UNTUK BUDIDAYA IKAN KERAPU MACAN (Epinephelus fuscoguttatu) DI PERAIRAN PULAU TEGAL KECAMATAN TELUK PANDAN KABUPATEN PESAWARAN

2018 ◽  
Vol 6 (2) ◽  
pp. 705 ◽  
Author(s):  
Glenn Valentino ◽  
Abdullah Aman Damai ◽  
Herman Yulianto
Keyword(s):  
Water Quality ◽  
Secondary Data ◽  
Fish Farming ◽  
Primary Data ◽  
Marine Aquaculture ◽  
Quality Of Waters ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Physical And Chemical

Tiger grouper is a marine aquaculture commodities that has good prospects to be developed. One of the areas that could potentially be used as a place of tiger grouper fish farming is the Tegal Island, Pesawaran. The purpose of this research was to analyze the level of water quality for fish farming tiger grouper (Epinephelus fuscoguttatus). This research was carried out in March-May 2017 descriptive analytic nature by doing the observations on the quality of waters which include physical and chemical parameters. The data that used in this research sourced from primary data about the suitability waters of the island while the secondary data is form in research location maps. There were 4 sampling stations which determined with global positioning system (GPS). The results showed that the water quality which is owned by the island's waters on station 1, 2, 3 and 4 were in the quite appropriate category for the cultivation of tiger grouper.

scholarly journals EFEKTIVITAS SERBUK DAUN TANAMAN KAYU MANIS (Cinnamomum burmanii) TERHADAP DIFERENSIAL LEUKOSIT DAN AKTIVITAS FAGOSITOSIS IKAN NILA (Oreochoromis niloticus) YANG DIINFEKSI Streptococcus agalactiae

2018 ◽  
Vol 1 (1) ◽  
pp. 40
Author(s):  
Sri Wulandari ◽  
Rahmad Jumadi ◽  
Firma Fika Rahmawati
Keyword(s):  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Phagocytic Activity ◽  
Challenge Test ◽  
Bacterial Disease ◽  
Leaf Powder ◽  
Alternative Measures ◽  
Monocyte Cell ◽  
And Control ◽  
Phagocytosis Activity

The main problem in the cultivation of tilapia is the attack of bacterial disease Streptococcosiscaused by Streptococcus agalactiae bacteria. Alternative measures to prevent the disease ofStreptocococis can be through the use of phytopharmaca materials. One of the ingredients ofphytopharmaca can be used is cinnamon plant. Cinnamon leaves contain several types of activesubstances such as tannins, eugenol, safrole, calcium oxalate, resin, saponins, tanners, andsinamaldehid. The objective of the study of effectiveness of leaf powder of cinnamon plant toleukocyte differential and phagocytic activity in tilapia (Oreochromis niloticus) infected byStreptococcus agalactiae is to know the influence and dosage of cinnamon leaf powder in feedtoLeukocyte Differentiation and Phagocytosis Activity Postcainfection Streptococcus agalactiaein tilapia (Oreochromis niloticus). This research used 3treatment 4 replication and control withdose K- = challenge test, K+ = without test challenge, A = dose 0,25%, B = dose 0,5%, C = 1%.The parameters observed are Leukocyte Differential and Phagocytosis Activity. Dosage 0.5%addition of leaf powder cinnamon plant on feed is the best dose. Giving of cinnamon leaf powderin feed influenced to increase of leukocyte difference especially on monocyte cell and neutrophilcell was significantly different (P>0,05) than K+ without addition of cinnamon leaf powderwhile phagocytic activity had an effect on Streptococcus agacatiae.

Hybridization of tiger grouper (Epinephelus fuscoguttatus ♀) x giant grouper (Epinephelus lanceolatus ♂) using cryopreserved sperm

Cryobiology ◽  
2020 ◽  
Vol 95 ◽  
pp. 84-89
Author(s):  
Bin Fan ◽  
Sen Yang ◽  
Le Wang ◽  
Xinghan Chen ◽  
Xiaochun Liu ◽  
...  
Keyword(s):  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper ◽  
Cryopreserved Sperm

scholarly journals Effects of Ascorbic Acid and β-1,3-Glucan on Survival, Physiological Response and Flesh Quality of Cultured Tiger Grouper (Epinephelus fuscoguttatus) during Simulated Transport in Water

Biology ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 37 ◽  
Author(s):  
Bo Wu ◽  
Qi Wang ◽  
Jie Cao ◽  
Jun Mei ◽  
Jing Xie
Keyword(s):  
Ascorbic Acid ◽  
Survival Rate ◽  
Stress Response ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein 70 ◽  
Flesh Quality ◽  
Epinephelus Fuscoguttatus ◽  
Tiger Grouper

Transport in water is the most common method for achieving high survival rates when transporting cultured fish in China; yet, transport success relies on proper water quality and conditions. This research was designed to explore the effects of ascorbic acid and β-1,3-glucan on survival, physiological responses, and flesh quality of farmed tiger grouper (Epinephelus fuscoguttatus) during simulated transport. The transport water temperature for live tiger grouper was 15 °C, which had the highest survival rate, the lowest stress response, and metabolic rate, and this will reduce the susceptibility to diseases. It is stated that β-1,3-glucan influences the changes of cortisol content, heat shock protein 70, IL-1β, and IgM transcription levels during simulated transport. Rather than using ascorbic acid alone (the A-group), β-1,3-glucan (3.2 mg/L) in the presence of ascorbic acid (25 mg/L) can effectively reduce the increase of transport-induced serum cortisol content, heat shock protein 70, and IL-1β, but stimulated IgM. 25 mg/L ascorbic acid and 3.2 mg/L β-1,3-glucan had no obvious effect on the nutritional indexes and flavor of live tiger grouper; however, these can effectively reduce the stress response, improve the innate immune activity, and ensure a higher survival rate.

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