scholarly journals EFEKTIVITAS SERBUK DAUN TANAMAN KAYU MANIS (Cinnamomum burmanii) TERHADAP DIFERENSIAL LEUKOSIT DAN AKTIVITAS FAGOSITOSIS IKAN NILA (Oreochoromis niloticus) YANG DIINFEKSI Streptococcus agalactiae

2018 ◽  
Vol 1 (1) ◽  
pp. 40
Author(s):  
Sri Wulandari ◽  
Rahmad Jumadi ◽  
Firma Fika Rahmawati
Keyword(s):  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Phagocytic Activity ◽  
Challenge Test ◽  
Bacterial Disease ◽  
Leaf Powder ◽  
Alternative Measures ◽  
Monocyte Cell ◽  
And Control ◽  
Phagocytosis Activity

The main problem in the cultivation of tilapia is the attack of bacterial disease Streptococcosiscaused by Streptococcus agalactiae bacteria. Alternative measures to prevent the disease ofStreptocococis can be through the use of phytopharmaca materials. One of the ingredients ofphytopharmaca can be used is cinnamon plant. Cinnamon leaves contain several types of activesubstances such as tannins, eugenol, safrole, calcium oxalate, resin, saponins, tanners, andsinamaldehid. The objective of the study of effectiveness of leaf powder of cinnamon plant toleukocyte differential and phagocytic activity in tilapia (Oreochromis niloticus) infected byStreptococcus agalactiae is to know the influence and dosage of cinnamon leaf powder in feedtoLeukocyte Differentiation and Phagocytosis Activity Postcainfection Streptococcus agalactiaein tilapia (Oreochromis niloticus). This research used 3treatment 4 replication and control withdose K- = challenge test, K+ = without test challenge, A = dose 0,25%, B = dose 0,5%, C = 1%.The parameters observed are Leukocyte Differential and Phagocytosis Activity. Dosage 0.5%addition of leaf powder cinnamon plant on feed is the best dose. Giving of cinnamon leaf powderin feed influenced to increase of leukocyte difference especially on monocyte cell and neutrophilcell was significantly different (P>0,05) than K+ without addition of cinnamon leaf powderwhile phagocytic activity had an effect on Streptococcus agacatiae.

scholarly journals Efikasi Vaksinasi pada Benih Ikan Nila (Oreochromis niloticus) dengan Metode Infiltrasi Hiperosmotik untuk Mencegah Infeksi Streptococcus agalactiae (THE EFFICACY OF VACCINATION ON TILAPIA SEEDS OF (Oreochromis niloticus) USING HYPEROSMOTIC INFILTRATION

2018 ◽  
Vol 18 (4) ◽  
pp. 634 ◽  
Author(s):  
Amalia Putri Firdausi ◽  
Sukenda Sukenda ◽  
Sri Nuryati
Keyword(s):  
Immune System ◽  
Antibody Level ◽  
Specific Antibody ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Challenge Test ◽  
Salinity Treatment ◽  
Post Vaccination ◽  
Randomized Design ◽  
And Control

The objective of this study was to evaluate the effectiveness of vaccination on tilapia seedlings resulted from the vaccinated parent by hyperosmotic infiltration method at four different salinity to prevent Streptococcus agalactiae bacterial infection. A total of 100 seeds aged 20 days from the vaccinated mother were immersed in four different salinity: 0 ppt (control), 10 ppt, 20 ppt, and 30 ppt for five minutes, then removed and transferred into vaccine-containing containers for 30 minutes. Further maintenance was performed in freshwater aquariums and at days 10, 20, and 30 post vaccination, the immune system performance: Relative Percent Survival (RPS) after the challenge test, specific antibody level, and lysozyme was observed. The study was conducted using a complete randomized design with three replications. The results showed that the 10 ppt salinity treatment gave the best results compared to the others and control. The final RPS (10 ppt) value was 84.72%, 66.49%, and 47.06%, on the 10th, 20th, and 30thh days of vaccination, respectively. Specific antibody levels (10 ppt) were 0.077, 0.078, and 0.077 and lysozyme 0.092, 0.084, and 0.032 at days 10, 20, and 30 post vaccination, respectively. The value of RPS and specific antibody level at 10 ppt salinity was significantly different (P <0.05) compared to the other treatments, while the lysozyme treatment activity of 10 ppt was not significantly different compared to the 20 ppt treatment. Vaccination using 10 ppt saline hyperosmotic infiltration can improve the performance of the immune system by improving maternal immune protection against Streptococcus agalactiae infection.

scholarly journals PENGARUH PENAMBAHAN SERBUK DAUN TANAMAN KAYU MANIS (Cinnamomum burmannii) PADA PAKAN TERHADAP PROFIL DARAH (KADAR HEMATOKRIT, KADAR HEMOGLOBIN, TOTAL LEUKOSIT DAN TOTAL ERITROSIT) IKAN NILA (Oreochromis niloticus) YANG DIINFEKSI Streptococcus agalactiae

2018 ◽  
Vol 1 (1) ◽  
pp. 24
Author(s):  
Sri Lestari ◽  
Firma Fika Rahmawati ◽  
Rahmad Jumadi
Keyword(s):  
Water Quality ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Chemical Compounds ◽  
Hemoglobin Level ◽  
Bacterial Disease ◽  
Hematocrit Level ◽  
Blood Profile ◽  
Leaf Powder ◽  
The Given

Bacterial disease like Streptococcosis which is caused by Streptoccoccusagalactiae becomesthe most important problem that often arise in the aquaculture business. The disease can be100% cause of death. One of the prevention efforts is using phytopharmaca in cinnamon leaf(Cinnamomunburmanni). This traditional herb is eco-friendly, safe for consumers and it does nothave any residue. Chemical compounds contained in cinnamon leaves have function asantioxidants, to increase metabolism, growth and non-specific immune response in fish. Thisstudy is to determine the effect and find the best dose of the addition of cinnamon leaf powder infood given to Tilapia that infected by Streptococcus agalactaiae. The tested dose is 0%, 0.25%,0.5%, 1%. Observed parameters were blood profile (hematocrit level, hemoglobin level, totalerythrocytes and total leukocytes) and water quality. The results were analyzed by ANOVA usingRAL with 95% credibility level. The results obtained in the study by adding cinnamon leafpowder to the given food to tilapia that was infected by Streptococcus agalactiae affect the bloodprofile (hematocrit level, hemoglobin level, total erythrocytes and total leukocytes). A dose of0.25% is the best dose for hematocrit level, hemogobline level and erythrocytes total. While thedose of 1% is the best dose for the total value of leukocytes. Doses of 0.25% and 1% can beapplied to tilapia culture to prevent Streptococcosis disease caused by Streptococcus agalactiaebacteria.

scholarly journals Bayesian genomic models boost prediction accuracy for survival to Streptococcus agalactiae infection in Nile tilapia (Oreochromus nilioticus)

2021 ◽  
Vol 53 (1) ◽  
Author(s):  
Rajesh Joshi ◽  
Anders Skaarud ◽  
Alejandro Tola Alvarez ◽  
Thomas Moen ◽  
Jørgen Ødegård
Keyword(s):  
Genomic Selection ◽  
Streptococcus Agalactiae ◽  
Nile Tilapia ◽  
Prediction Accuracy ◽  
Predictive Accuracy ◽  
Challenge Test ◽  
Bacterial Disease ◽  
Nucleotide Polymorphisms ◽  
Genomic Selection Model ◽  
Selection For

Abstract Background Streptococcosis is a major bacterial disease in Nile tilapia that is caused by Streptococcus agalactiae infection, and development of resistant strains of Nile tilapia represents a sustainable approach towards combating this disease. In this study, we performed a controlled disease trial on 120 full-sib families to (i) quantify and characterize the potential of genomic selection for survival to S. agalactiae infection in Nile tilapia, and (ii) identify the best genomic model and the optimal density of single nucleotide polymorphisms (SNPs) for this trait. Methods In total, 40 fish per family (15 fish intraperitoneally injected and 25 fish as cohabitants) were used in the challenge test. Mortalities were recorded every 3 h for 35 days. After quality control, genotypes (50,690 SNPs) and phenotypes (0 for dead and 1 for alive) for 2472 cohabitant fish were available. Genetic parameters were obtained using various genomic selection models (genomic best linear unbiased prediction (GBLUP), BayesB, BayesC, BayesR and BayesS) and a traditional pedigree-based model (PBLUP). The pedigree-based analysis used a deep 17-generation pedigree. Prediction accuracy and bias were evaluated using five replicates of tenfold cross-validation. The genomic models were further analyzed using 10 subsets of SNPs at different densities to explore the effect of pruning and SNP density on predictive accuracy. Results Moderate estimates of heritabilities ranging from 0.15 ± 0.03 to 0.26 ± 0.05 were obtained with the different models. Compared to a pedigree-based model, GBLUP (using all the SNPs) increased prediction accuracy by 15.4%. Furthermore, use of the most appropriate Bayesian genomic selection model and SNP density increased the prediction accuracy up to 71%. The 40 to 50 SNPs with non-zero effects were consistent for all BayesB, BayesC and BayesS models with respect to marker id and/or marker locations. Conclusions These results demonstrate the potential of genomic selection for survival to S. agalactiae infection in Nile tilapia. Compared to the PBLUP and GBLUP models, Bayesian genomic models were found to boost the prediction accuracy significantly.

scholarly journals Efficacy of inactive bivalent and trivalent Streptococcus agalactiae bacteria (biotype 1 & 2) vaccines on tilapia, Oreochromis niloticus

2021 ◽  
Vol 322 ◽  
pp. 02014
Author(s):  
Taukhid ◽  
Angela M. Lusiastuti ◽  
Septyan Andriyanto ◽  
Desy Sugiani ◽  
Tuti Sumiati ◽  
...  
Keyword(s):  
Streptococcus Agalactiae ◽  
Vaccine Efficacy ◽  
Oreochromis Niloticus ◽  
Challenge Test ◽  
Fish Disease ◽  
Post Vaccination ◽  
Relative Percentage ◽  
Bivalent Vaccine ◽  
Main Indicator ◽  
Trivalent Vaccine

Streptococcosis is a significant fish disease impacting tilapia culture in Indonesia, causing losses estimated up to IDR 15.0 billion annually. This study aims to assess the efficacy of bivalent and trivalent vaccines containing Streptococcus agalactiae bacteria on tilapia. The formula of the bivalent vaccine contains 75% of S01-196-16 and 25% of N14G isolates (v/v). Trivalent vaccine contains 30%, 35%, and 35% of N14G, NP1050, and SG01-16 isolates (v/v), respectively. A challenge test assessed the efficacy of the vaccines, and it was carried out at 30, 90, and 150 days post-vaccination by artificially infection at LD60. Selected bacteria isolate to be appointed in the challenge test are N14G (biotype 2) and S01-196-16 (biotype 1). Relative Percentage of Survival (RPS) was used as the main indicator of vaccine efficacy. The results revealed that the highest RPS of a bivalent vaccine against S. agalactiae (S01-196-16) was achieved at the first challenge (61.84%), and trivalent vaccine against S. agalactiae (N14G) and S. agalactiae (S01-196-16) was achieved at the first challenge (61.53% and 76.20%, respectively). Bivalent and trivalent S. agalactiae bacteria vaccines are promising “tools” to control streptococcosis on tilapia.

scholarly journals Penggunaan bakteri probiotik untuk pencegahan infeksi bakteri Streptococcus agalactiae pada ikan Nila, Oreochromis niloticus

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Aisin Umasugi ◽  
Reiny A. Tumbol ◽  
Reni L. Kreckhoff ◽  
Henky Manoppo ◽  
Novie P.L. Pangemanan ◽  
...  
Keyword(s):  
Statistical Analysis ◽  
Survival Rate ◽  
Streptococcus Agalactiae ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Challenge Test ◽  
Probiotic Bacteria ◽  
Growth And Survival ◽  
Significant Difference ◽  
Materials Used

This study aimed to evaluate the effect of probiotic on growth and survival of tilapia (Oreochromis niloticus) against Streptococcus agalactiae infection. The test materials used were commercial probiotic bacteria and Streptococcus agalactiae. The probiotic bacteria were administered by mixing into the feed with a dose of 0 mL (without probiotics), 10 mL / kg of feed, 15 mL / kg of feed and 20 mL / kg of feed.  Feed was given 3 times a day at 08.00, 12.00 and 16.00 for 21 days. After that, the challenge test was done with Streptococcus  agalactiae bacteria by cohabitation. Bacteria with a density of 107 cells / mL were mixed into the water and allowed for 2 hours. The result showed that B treatment (10 mL / kg of feed) gave the best result with 93,33 percent of  survival rate. Statistical analysis showed that the survival of fish in treatments B, C and D was significantly different from treatment A (P <0.1). However, there was no significant difference between treatments B, C, and D. It was also found that the addition of probiotic in feed did not affect the growth of Nile tilapia (Orechromis niloticus). Keywords : Probiotic bacteria,  nile tilapia,  Streptococcus  agalactiae, growth,  infection

scholarly journals Specific Immune Response Kinetics and Mortality Patterns of Tilapia Oreochromis niloticus on Post-Cocktail Vaccination Period against the Infection of Aeromonas hydrophila and Streptococcus agalactiae

Omni-Akuatika ◽  
2017 ◽  
Vol 13 (2) ◽  
Author(s):  
Sukenda Sukenda ◽  
Tuti Sumiati ◽  
Sri Nuryati ◽  
Angela Mariana Lusiastuti ◽  
Dendi Hidayatullah
Keyword(s):  
Immune Response ◽  
Streptococcus Agalactiae ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Challenge Test ◽  
Specific Immune Response ◽  
Single Infection ◽  
Control Group ◽  
Antibody Levels ◽  
Kinetics Of

ABSTRACTFish vaccination aims to induce a specific immune response indicated by an increase of antibodies in vaccinated fish. However, in accordance with time the presence of antibodies will continue to decline. The purpose of this study was to determine the kinetics of specifik immune response and trend mortality against Aeromonas hydrophila and Streptococcus agalactiae on tilapia following vaccination with cocktail vaccine. Fish vaccinated through immersion for 30 minutes in a solution of diluted vaccine. Challenge test was performed for three periods, on day 22, 50, and 78 post-vaccination, fish were challenged with single infection of A. hydrophila 108 cfu. mL-1 and S. agalactiae 104 cfu. mL-1 and co-infection of both bacteria by intraperitoneal. During rearing, the blood fish were taken for determining of serum antibodies, and its  measured by ELISA. The results showed that the concentration of specific antibodies vaccinated fish were significantly higher than the control. The basal antibody levels of A. hydrophila before vaccination were higher than S. agalactiae with OD of 0.104 and 0.069 respectively. The maximum  antibody  response  was  reached  within  70  days  of  the  A. hydrophila OD= 0.264 and 56 days against S. agalactiae OD= 0.188. The mortality rate in the control group was significantly higher than vaccinated on all types and each challenge test period. The trend of mortality due to a single infection of A. hydrophila and co-infections occur more quickly than by S. agalactiae. Lowest mortality occurred in the vaccinated group at 50 day tested challenge.Keywords: kinetics antibody, Aeromnas hydrophila, Streptococcus agalactiae, Oreochromis niloticus

scholarly journals Resistance of Fry from Vaccinated Mother of Gift Tilapia (Oreochromis niloticus Linn.) to Artificial Infection of Streptococcus iniae

2007 ◽  
Vol 3 (1) ◽  
pp. 37 ◽  
Author(s):  
I. Nur ◽  
. Sukenda ◽  
D. Dana
Keyword(s):  
Body Weight ◽  
Oreochromis Niloticus ◽  
Body Fluid ◽  
Challenge Test ◽  
The Body ◽  
Streptococcus Iniae ◽  
Soluble Extract ◽  
Before And After ◽  
Antibody Titers ◽  
And Control

<p>An investigation was made to study the efficacy of dosage and time of administration in maternal vaccination. Mothers of nile tilapia were vaccinated by intraperitoneal injection with adjuvant-heat killed <em>Streptococcus iniae </em>at one and two weeks after spawning at the dosages of 0,2 ml/kg and 0,4 ml/kg body weight. Unvaccinated mothers were used as control. Agglutinating antibody titers in the blood plasma of mothers before and after spawning, eggs soluble extract, the body fluid of fry at 5, 10 and 15 days post hatching (DPH) were examined. The protective immunity of fry was tested by challenge test, the survival rate (SR) and the relative percent survival (RPS) offish within 7 days was observed. There was no difference in the antibody level of mothers, eggs soluble extract, and the body fluid of fry at 5, 10 and 15 DPH from vaccinated mothers at one or two weeks after spawning. However, antibody of mothers, eggs and fry from vaccinated mothers at the dosage of 0,4 ml/kg body weight were higher than the dosage of 0,2 ml/kg body weight and control. Antibody titre. of fry of vaccinated mothers at 5, 10 and 15 DPH were (-log2=2,88), (-log2=2,53) and (-log2=2,07) respectively, while SR were 89%, 94% and 92% respectively. SR of control fry were lower 7%, 10% and 12% respectively than fry from vaccinated mother; meanwhile RPS were 47%. 76% and 77% respectively.</p> <p>Key words: Maternal immunity. <em>Streptococcus iniae, Oreochromis niloticus, </em>vaccination, antibody</p> <p> </p> <p>ABSTRAK</p> <p>Suatu studi tentang efikasi dari dosis dan waktu pemberian vaksin pada vaksinasi lewat induk dilakukan. Induk ikan nila <em>(Oreochromis niloticus) </em>diberi vaksin melalui injeksi intraperitoneal dengan "adjuvant-heat killed <em>Streptococcus iniae" </em>satu minggu sesudah memijah dan dua minggu sesudah memijah dengan dosis 0,2 ml/kg dan 0,4 ml/kg bobot tubuh. Aglutinasi titer antibodi dari plasma darah induk sebelum dan sesudah memijah, ekstrak terlarut telur, dan cairan tubuh pada 5, 10 dan 15 hari setelah menetas (DPH) diperiksa. Imunitas dari larva diuji dengan uji tantang, kelangsungan hidup (SR) dalam 7 hari setelah uji tantang diamati. Hasil menunjukkan bahwa tidak ada perbedaan pada level antibodi dari induk, ekstrak terlarut telur dan cairan tubuh dari larva pada 5, 10 dan 15 DPH dari induk yang diberi vaksin pada satu atau dua minggu setelah memijah. Akan tetapi, level antibodi induk, ekstrak terlarut telur dan cairan tubuh larva dari induk-induk yang diberi vaksin dengan dosis 0.4 ml/kg bobot tubuh lebih tinggi dari 0.2 ml/kg bobot tubuh dan kontrol. Level antibodi dari larva yang berasal dari induk yang diberi vaksin dengan dosis 0.2 ml/kg, 0.4 ml/kg dan kontrol adalah (-log2=2.5), (-log2=2.9) dan (-log2=2). Sedangkan kelangsungan hidupnya masing-masing 95%, 94% dan 84%.</p> Kata kunci: Imunitas induk. <em>Streptococcus iniae, Oreochromis niloticus, </em>vaksinasi, antibodi

scholarly journals Bayesian genomic models boost prediction accuracy for resistance against Streptococcus agalactiae in Nile tilapia (Oreochromus nilioticus)

2020 ◽  
Author(s):  
Rajesh Joshi ◽  
Anders Skaaurd ◽  
Alejandro Tola Alvarez ◽  
Thomas Moen ◽  
Jørgen Ødegård
Keyword(s):  
Genomic Selection ◽  
Streptococcus Agalactiae ◽  
Nile Tilapia ◽  
Prediction Accuracy ◽  
Challenge Test ◽  
Practical Implementation ◽  
Bacterial Disease ◽  
Genetic Strains ◽  
Genomic Selection Model ◽  
Selection For

AbstractStreptococcosis due to Streptococcus agalactiae is a major bacterial disease in Nile tilapia, and development of the resistant genetic strains can be a sustainable approach towards combating this problematic disease. Thus, a controlled disease trial was performed on 120 full-sib families to i) quantify and characterize the potential of genomic selection for S. agalactiae resistance in Nile tilapia and to ii) select the best genomic model and optimal SNP-chip for this trait.In total, 40 fish per family (15 fish intraperitoneally injected and 25 fish as cohabitants) were selected for the challenge test and mortalities recorded every 3 hours, until no mortalities occurred for a period of 3 consecutive days. Genotypes (50,690 SNPs) and phenotypes (0 for dead and 1 for alive) for 2472 cohabitant fish were available. The pedigree-based analysis utilized a deep pedigree, going 17 generations back in time. Genetic parameters were obtained using various genomic selection models (GBLUP, BayesB, BayesC, BayesR and BayesS) and traditional pedigree-based model (PBLUP). The genomic models were further analyzed using 10 different subsets of SNP-densities for optimum marker density selection. Prediction accuracy and bias were evaluated using 5 replicates of 10-fold cross-validation.Using an appropriate Bayesian genomic selection model and optimising it for SNP density increased prediction accuracy up to ∼71%, compared to a pedigree-based model. This result is encouraging for practical implementation of genomic selection for S. agalactiae resistance in Nile tilapia breeding programs.

The effect of vaccination in controling bacterial disease caused by Aeromonas hydrophila in nile tilapia (Oreochromis niloticus)

2014 ◽  
pp. 27
Author(s):  
Arne A Ratulangi ◽  
Reiny Tumbol ◽  
Hengky Manoppo ◽  
Henneke Pangkey
Keyword(s):  
Survival Rate ◽  
Aeromonas Hydrophila ◽  
Oreochromis Niloticus ◽  
Nile Tilapia ◽  
Bacterial Disease ◽  
Immersion Method ◽  
Nile Tilapia Oreochromis Niloticus ◽  
Different Ages ◽  
Motile Aeromonas Septicaemia

This study aims to apply vaccination against bacterial disease. The purpose of vaccination is to trigger the immune respone both non-specific and specific of fish against bacteria Motile Aeromonas Septicaemia (MAS) caused by Aeromonas hydrophila. The vaccination for fish with different ages: 2-3 weeks and 5 weeks were done using immersion method. The fish were re-vaccinated (booster) after two weeks of the first vaccination. The survival rate was < 50 % for juveniles 2-3 weeks and > 50% for juveniles 5 weeks. Survival rate for juveniles 5 weeks was higher than juveniles of 2-3 weeks. This shows that organs of juveniles of 5 weeks were more complete than the 2-3 weeks juveniles. The age of fish is one of the important factors for successfully vaccination. Penelitian ini bertujuan untuk melakukan penerapan vaksinasi terhadap penyakit bakterial. Vaksinasi ditujukan untuk merangsang respon kekebalan non- spesifik dan spesifik pada tubuh ikan terhadap penyakit Motile Aeromonas Septicaemia (MAS) yang disebabkan oleh bakteri Aeromonas hydrophila. Vaksin diberikan pada benih ikan nila yang berbeda umur yaitu 2-3 minggu dan 5 minggu dengan menggunakan metode perendaman. Ikan divaksinasi ulang (booster) setelah 2 (dua) minggu dari vaksinasi yang pertama. Prosentase kelangsungan hidup < 50 % untuk benih umur 2-3 minggu dan > 50% untuk benih umur 5 minggu. Jumlah kematian benih umur 2-3 minggu lebih tinggi dari 5 minggu. Hal ini menunjukkan bahwa fungsi organ benih umur 5 minggu telah lebih lengkap dari pada benih umur 2-3 minggu. Umur ikan merupakan salah satu faktor penting penentu keberhasilan suatu kegiatan vaksinasi.

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