scholarly journals The Chitosan-Sodium Alginate Submicro Particles Loading Herbal of Ethanolic Exract of Leaves Senna Alata. L for Curing of Bacterial Infection on Skin

2020 ◽  
Vol 5 (3) ◽  
pp. 85
Author(s):  
Mardiyanto Mardiyanto ◽  
Indah Sholihah ◽  
Thio Gunawan Jaya
Keyword(s):  
Sodium Alginate ◽  
Leaf Extract ◽  
Test Group ◽  
Ethanol Extract ◽  
X Ray Diffraction ◽  
X Ray ◽  
Acne Lesion ◽  
Optimum Formula ◽  
Senna Alata

Abstract Mardiyanto 1*, Indah Solihah 1, and Thio Gunawan Jaya 1 1Department of Pharmacy Faculty of Science Sriwijaya University *Corresponding Author : [email protected] This research was performed to detect the activity of the optimum formula of chitosan and sodium alginate submicro particles loading of the ethanol extract of leaves ketepeng cina (Senna alata L.) to P. acne growth. Submicro manufactured particles was used three variations of the formula from three of volume used of calcium chloride (CaCl2): 20, 40, and 100 mL based on ionic gelation method. The results obtained of %EE in formulas 1, 2 and 3 were 78.56%, 81.71%, and 77.48%. Formula 2 with a value of % EE of 81.71% was used as an optimum formula which indicates that the particles are well protected by chitosan and sodium alginate polymers so as to prevent particle damage during the homogenization process. The results of the diameter measurements showed that the optimal formula enters in the submicro particle range with the value obtained is 525,9455 nm. The submicro formula of chitosan alginate particles loading the ketepeng cina leaf ethanol extract was barely homogeneous which based on the results obtained by the PSA was 0.433 of PDI. The zeta potential value was +3.5 mV. The results of X-ray diffraction analysis or X ̶ Ray Diffraction produce a pattern shaped amorph with the resulting peak does not have a wide distance and pattern. In vivo testing using ketepeng cina leaf extract as submicro was decreased the P. acne lesion faster than using only ketepeng cina leaf extract because the submicro particle preparation has a small particle that is below to 600 nm making it easier to penetrate the skin pores to reach the target. The results of the analysis of the curing of many lesions on the skin of mice have a significance value of homogeneity. The one-way ANOVA test of < 0.05 was a significant effect of the test group on the decreasing in the number of lesions on the skin of the mouse.  

Preliminary Study of Alginates Extracted from Brown Algae (Sargassum sp.) Available in Madura Island as Composite Based Hydrogel Materials

2019 ◽  
Vol 964 ◽  
pp. 240-245 ◽  
Author(s):  
Amaliya Rasyida ◽  
Thalyta Rizkha Pradipta ◽  
Sigit Tri Wicaksono ◽  
Vania Mitha Pratiwi ◽  
Yeny Widya Rakhmawati
Keyword(s):  
Sodium Alginate ◽  
Brown Algae ◽  
Extraction Process ◽  
Composition Analysis ◽  
3D Printer ◽  
X Ray Diffraction ◽  
X Ray ◽  
3D Printed ◽  
Preliminary Study

Utilization of brown algae especially in Madura, where it’s close to Surabaya, only limited for food. This become a reference for developing and increasing the potential of this algae by extracting one of the ingredients, namely alginate. This paper deals with the characterization of sodium alginate extracted from sargassum sp. using modified-purified calcium routes. The extracted sodium alginate will be further used as composite hydrogel materials and compared with commercial sodium alginate. Hereafter, the synthesized composite is expected to be bio-ink for 3d printer. Chemical composition analysis were analyzed using X-Ray Fluorosense (XRF) followed by Fourier-transform infrared spectroscopy (FTIR) analysis to identify the functional group of composite and X-Ray Diffraction (XRD). Furthermore, viscosity bath is performed to compare the viscosity of extracted and commercial one. The result shows that modified-purified calcium routes in the extraction process of sodium alginate is desirable for improving their properties. Interestingly enough, with the goal of using it as bio-ink in 3d printed fabrication, the synthesized composite shows viscosity, 300 cSt, which meets the criteria for bio-ink in 3d printer.

Effects of dietary alteration of bicarbonate and magnesium on rat bone

1986 ◽  
Vol 250 (2) ◽  
pp. F302-F307 ◽  
Author(s):  
J. M. Burnell ◽  
C. Liu ◽  
A. G. Miller ◽  
E. Teubner
Keyword(s):  
Crystal Structure ◽  
Bone Formation ◽  
X Ray Diffraction ◽  
X Ray ◽  
Growing Rats ◽  
Final Composition ◽  
Rat Bone

To study the effects of bicarbonate and magnesium on bone, mild acidosis and/or hypermagnesemia were produced in growing rats by feeding ammonium chloride and/or magnesium sulfate. Bone composition, quantitative histomorphometry, and mineral x-ray diffraction (XRD) characteristics were measured after 6 wk of treatment. The results demonstrated that both acidosis (decreased HCO3) and hypermagnesemia inhibited periosteal bone formation, and, when combined, results were summative; and the previously observed in vitro role of HCO3- and Mg2+ as inhibitors of crystal growth were confirmed in vivo. XRD measurements demonstrated that decreased plasma HCO3 resulted in larger crystals and increased Mg resulted in smaller crystals. However, the combined XRD effects of acidosis and hypermagnesemia resembled acidosis alone. It is postulated that the final composition and crystal structure of bone are strongly influenced by HCO3- and Mg2+, and the effects are mediated by the combined influence on both osteoblastic bone formation and the growth of hydroxyapatite.

scholarly journals Bio Synthesis and Characterization of Silver Nanoparticles Using Lagenaria siceraria Leaf Extract and their Antibacterial Activity

2014 ◽  
Vol 38 ◽  
pp. 35-45 ◽  
Author(s):  
B. Anandh ◽  
A. Muthuvel ◽  
M. Emayavaramban
Keyword(s):  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Face Centered Cubic ◽  
Lagenaria Siceraria ◽  
Human Pathogens ◽  
X Ray Diffraction ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Ft Ir ◽  
Uv Visible

The present investigation demonstrates the formation of silver nanoparticles by the reduction of the aqueous silver metal ions during exposure to the Lagenaria siceraria leaf extract. The synthesized AgNPs have characterized by UV-visible spectroscopy, X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) techniques. AgNPs formation has screened by UV-visible spectroscopy through colour conversion due to surface plasma resonance band at 427 nm. X-ray diffraction (XRD) confirmed that the resulting AgNPs are highly crystalline and the structure is face centered cubic (fcc). FT-IR spectrum indicates the presence of different functional groups present in the biomolecules capping the nanoparticles. Further, inhibitory activity of AgNPs and leaf extract were tested against human pathogens like gram-pastive (Staphylococcus aureus, Bacillus subtilis), gram-negative (Escherichia coli and Pseudomonas aeruginosa). The results indicated that the AgNPs showed moderate inhibitory actions against human pathogens than Lagenaria siceraria leaf extract, demonstrating its antimicrobial value against pathogenic diseases

POTENTIAL OF ETHANOL EXTRACT OF MAHKOTA DEWA LEAVES (PHALERIA MACROCARPA (SCHECFF.) BOERL.) TO INHIBIT INFLAMMATION IN MOUSE DISTAL COLON INDUCED BY DEXTRAN SODIUM SULFATE (DSS) AND AZOXYMETHANE (AOM)

2020 ◽  
pp. 101-105
Author(s):  
MUHAMMAD ILHAM DHIYA RAKASIWI ◽  
KUSMARDI KUSMARDI ◽  
ARI ESTUNINGTYAS ◽  
ARYO TEDJO
Keyword(s):  
Leaf Extract ◽  
Histopathological Examination ◽  
Ethanol Extract ◽  
Distal Colon ◽  
Goblet Cells ◽  
Sodium Sulphate ◽  
Dextran Sodium Sulphate ◽  
Phaleria Macrocarpa ◽  
Hematoxylin Eosin

Objective: To demonstrates the ability of P. macrocarpa leaf extract to reduce inflammation of the distal colon in DSS/AOM-induced mice. Methods: In vivo experimental research using Balb/c mice induced by 0.2 ml azoxymethane (AOM) 0.1% once and 1% dextran sodium sulphate (DSS) for one week; additionally, ethanol extract of P. macrocarpa leaves, 25 mg and 50 mg, and 0.84 mg acetosal were given orally. The mice were sacrificed after 20 w. Histopathological examination (hematoxylin-eosin staining) was conducted by counting the average number of goblet cells per crypt, inflammatory focus and angiogenesis. Results: Ethanol extract of P. macrocarpa leaves was able to prevent the decrease in the number of goblet cells (p<0.05). However, the administration of ethanol P. macrocarpa leaf extract could not reduce focal inflammation and angiogenesis in inflammation of the distal colon. Conclusion: Ethanol extract of the Mahkota Dewa leaves is able to prevent inflammation of the distal colon by preventing the decrease in the number of goblet cells.

Thiosemicarbazonate complexes with affinity for amyloid-β fibers: synthesis, characterization and biological studies

2019 ◽  
Vol 11 (19) ◽  
pp. 2527-2546 ◽  
Author(s):  
Arantxa Pino-Cuevas ◽  
Paula D Raposinho ◽  
Célia Fernandes ◽  
António Paulo ◽  
Ulrich Abram ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Blood Brain Barrier ◽  
Free Ligand ◽  
Amyloid Β ◽  
Molecular Structures ◽  
X Ray Diffraction ◽  
X Ray ◽  
Biological Studies ◽  
Similar Affinity

Aim: Obtain radioimages of amyloid-β fibers using 99mTc-complexes. Methodology: Tridentate thiosemicarbazone and thiocarbonohydrazone ligands containing fragments (stilbene, azobenzene, benzothiazole or benzoxazole) with affinity for amyloid-ß fibers and its Re(I) complexes have been prepared. The molecular structures of several ligands and complexes were determined by x-ray diffraction. Binding affinity studies toward Aß1-42 fibers were performed for the ligands and Re(I) complexes. The ability of formation of some 99mTc(I) complexes, their biodistribution and in vivo stability have been established. Results & conclusion: Complexes of stilbene and benzothiazole thiosemicarbazonates show similar affinity for amyloid-β fibers to the free ligand. These 99mTc complexes present a reasonable in vivo stability and a low capability to cross the blood–brain barrier although not sufficient to brain amyloid imaging.

In Vivo Biological Behavior of Calcium Phosphate Cements with Different Ca/P Ratio

2008 ◽  
Vol 396-398 ◽  
pp. 237-240
Author(s):  
Sybele Saska ◽  
N.S. Nunes ◽  
Emelly Aveiro ◽  
Cristina A.C. Pavan ◽  
S.H. Santagneli ◽  
...  
Keyword(s):  
Thermogravimetric Analysis ◽  
31P Nmr ◽  
Phosphate Buffer Solution ◽  
Biological Behavior ◽  
X Ray Diffraction ◽  
Buffer Solution ◽  
X Ray ◽  
Calcium Phosphate Cements ◽  
Subcutaneous Tissues

Bioceramics with different Ca/P ratio were prepared from a mechanical mixture of NaPO3, CaCO3, Ca(OH)2 and phosphate buffer solution and implanted in rats subcutaneous tissues. The cements were characterized by Thermogravimetric analysis (TG-TDA), X-ray diffraction and 31P-NMR. The implant sites were excised after 1, 4 and 16 weeks, fixed, dehydrated, included in paraffin wax for serial cutting and examined under the light transmitted microscope. They were biocompatible and biodegradable when implanted in rat subcutaneous. None of the materials induced ectopic osteogenesis. According to the results, the studied materials seem to be able for manufacturing reabsorbable bone implants.

Strategy of an in vivo Study of the Evolution of Drugs via X-Ray Diffraction with Synchrotron Radiation

2018 ◽  
Vol 82 (7) ◽  
pp. 895-897
Author(s):  
M. R. Sharafutdinov ◽  
B. P. Tolochko ◽  
K. E. Kuper ◽  
A. I. Ancharov
Keyword(s):  
Synchrotron Radiation ◽  
In Vivo Study ◽  
X Ray Diffraction ◽  
X Ray
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