scholarly journals Impact of amphiphilic compounds on post-hypertonic shock of human erythrocytes

2019 ◽  
Keyword(s):  
Protective Effect ◽  
Hemolytic Activity ◽  
Hypertonic Solution ◽  
Ice Melting ◽  
Minimum Level ◽  
Experimental Conditions ◽  
Hypertonic Medium ◽  
Amphiphilic Compounds ◽  
Sodium Decyl Sulfate ◽  
Hypertonic Shock

When the cryopreserved erythrocytes are thawed, with the ice melting the extracellular hypertonic medium changes to isotonic one, resulting in post-hypertonic cell lysis development. Under experimental conditions, the post-hypertonic shock of erythrocytes simulates the influence of cryodamage factors, acting at the erythrocyte thawing stage, as well as when the cells, cryopreserved under protection of penetrating cryoprotectant are transferred into bloodstream. Post-hypertonic shock of erythrocytes was carried out by transferring the cells from a hypertonic solution contained 1.65 mol/l NaCl (dehydration medium) into an isotonic one with 0.15 mol/l NaCl (rehydration medium) at 0ºС. The effect of specimens of various classes of amphiphilic compounds (anionic sodium decyl sulfate, non-ionic decyl-β,D-glucopyranoside, and cationic chlorpromazine) on the human erythrocyte sensitivity to post-hypertonic shock, was studied. Amphiphilic substances were supplemented into rehydration medium prior to cell introduction into it. It was shown that under post-hypertonic shock of erythrocytes, all the studied amphiphilic substances, when used in efficient concentrations, manifested a high anti-hemolytic activity (at the level of 70%). A comparative study of the efficiency of amphiphilic substances under post-hypertonic shock of erythrocytes showed differences in size of the plateau (the concentration range of amphiphilic compound, within the limits of which the minimum level of erythrocyte hemolysis was observed). Thus, it was found that for non-ionic decyl-β,D-glucopyranoside the plateau was 3 times more than for anionic sodium decyl sulfate and cationic chlorpromazine. The minimum efficient concentration for sodium decyl sulfate and the maximum one for decyl-β,D-glucopyranoside under post-hypertonic shock of erythrocytes were revealed. It is assumed that the revealed protective effect of amphiphilic compounds under post-hypertonic shock of erythrocytes is associated with their capability to integrate into membrane. This entails an increase in the surface area of the membrane and, therefore, the critical hemolytic volume of cell, which allows it to swell to a larger volume.

scholarly journals GPR91 Receptor Mediates Protection against Doxorubicin-Induced Cardiotoxicity without Altering Its Anticancer Efficacy. An In Vitro Study on H9C2 Cardiomyoblasts and Breast Cancer-Derived MCF-7 Cells

Cells ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 2177
Author(s):  
Matthieu Dallons ◽  
Esma Alpan ◽  
Corentin Schepkens ◽  
Vanessa Tagliatti ◽  
Jean-Marie Colet
Keyword(s):  
Breast Cancer ◽  
Oxidative Stress ◽  
Protective Effect ◽  
Cancer Cells ◽  
1H Nmr ◽  
Breast Cancer Cells ◽  
Extracellular Fluid ◽  
H9c2 Cells ◽  
Experimental Conditions ◽  
Mcf 7

Doxorubicin (DOX) is an anticancer drug widely used in oncology, especially for breast cancer. The main limitation of DOX treatment is its cardiotoxicity due to the cumulative dose. Clinically, DOX-induced cardiomyopathy develops as a progressive heart failure caused by a progressive cardiomyocyte’s death. For long, the oxidative stress induced by DOX was considered as the main toxic mechanism responsible for heart damage, but it is now controverted, and other processes are investigated to develop cardioprotective strategies. Previously, we studied DOX-induced cardiotoxicity and dexrazoxane (DEX), the only cardioprotective compound authorized by the FDA, by 1H-NMR metabonomics in H9C2 cells. We observed an increased succinate secretion in the extracellular fluid of DEX-exposed cardiomyocytes, a finding that led us to the hypothesis of a possible protective role of this agonist of the GPR91 receptor. The objective of the present work was to study the effect of succinate (SUC) and cis-epoxysuccinate (cis-ES), two agonists of the GPR91 receptor, on DOX-induced cardiotoxicity to H9C2 cells. To this purpose, several toxicity parameters, including cell viability, oxidative stress and apoptosis, as well as the GPR91 expression, were measured to assess the effects of DEX, SUC and cis-ES either alone or in combination with DOX in H9C2 cells. A 1H-NMR-based metabonomic study was carried out on cellular fluids collected after 24 h to highlight the metabolic changes induced by those protective compounds. Moreover, the effects of each agonist given either alone or in combination with DOX were evaluated on MCF-7 breast cancer cells. GPR91 expression was confirmed in H9C2 cells, while no expression was found in MCF-7 cells. Under such experimental conditions, both SUC and cis-ES decreased partially the cellular mortality, the oxidative stress and the apoptosis induced by DOX. The SUC protective effect was similar to the DEX effect, but the protective effect of cis-ES was higher on oxidative stress and apoptosis. In addition, the metabonomics findings pointed out several metabolic pathways involved in the cardioprotective effects of both GPR91 agonists: the stimulation of aerobic metabolism with glucose as the main fuel, redox balance and phospholipids synthesis. Finally, none of the GPR91 agonists jeopardized the pharmacological effects of DOX on MCF-7 breast cancer cells.

scholarly journals Impact of membranetropic compounds on the sensitivity of mammalian erythrocytes to the posthypertonic shock action

2020 ◽  
pp. 31-38
Author(s):  
O Chabanenko ◽  
◽  
N Yershova ◽  
N Orlova ◽  
N Shpakova ◽  
...  
Keyword(s):  
Critical Micelle Concentration ◽  
Defect Formation ◽  
Phospholipid Composition ◽  
Erythrocyte Membranes ◽  
Minimum Level ◽  
Rat Erythrocytes ◽  
Amphiphilic Compounds ◽  
Antihemolytic Activity ◽  
Mammalian Erythrocyte ◽  
Shock Action

The effect of cationic trifluoperazine (TFP) and nonionic decyl-β,D-glucopyranoside (DGP) on the sensitivity of human, rabbit and rat erythrocytes to the action of posthypertonic shock (PHS) at 0 °C was studied in this research. Trifluoperazine shows a high antihemolytic activity under conditions of PHS of human and animal erythrocytes at slight differences of values of effective concentrations. The value of antihemolytic activity of TFP for human and rabbit erythrocytes is ~ 60 %, and for rat cells the efficiency of this compound is approximately 1.4 times higher. The values of antihemolytic activity of DGP under PHS conditions of human and rat erythrocytes are comparable and amounts to 62 and 66 %, respectively. Significant differen­ces of this parameter (72 %) were found for rabbit cells compared with human erythrocytes. It was found that the size of plateau (the range of concentrations of amphiphilic compounds within the minimum level of erythrocyte hemolysis was observed) cationic TFP and nonionic DGP are significantly different. Thus, TFP has a narrow plateau (100–200 μmol/L), while DGP has a rather wide one (400–1600 μmol/L). In addition, a shift of the plateau concentrations of DGP to the region of higher values compared with TFP is observed, which is probably due to the fact that the value of the critical micelle concentration DGP is higher than TFP. Moreover, a shift of plateau concentrations of DGP to the region of higher values compared with TFP is observed, that is probably due to the fact that the value of the critical micelle concentration DGP is higher than TFP one. It was established that under PHS conditions of human erythrocyte, both compounds (TFP and DGP) show a commensurate antihemolytic activity. At the same time, for rabbit cells, DGP is more effective compared with TFP, and for rat erythrocytes, on the contrary, the efficiency of TFP is higher than DGP. This may be due to differences in the phospholipid composition of mammalian erythrocyte membranes. The results suggest that under PHS conditions the efficacy of membrane-tropic compounds is most likely due to their ability to incorporate into membrane to the defect formation areas, and thus significantly increase the critical hemolytic volume of cells, as a result, prevent their destruction.

scholarly journals Investigation of Inhibitor Polyphosphate Properties for Rotating Steel Disk Electrode in Potassium Nitrate Solution

2016 ◽  
Vol 2 (2) ◽  
pp. 167
Author(s):  
E.A. Dorokhova ◽  
B.D. Burkitbaeva ◽  
I.B. Melsitova ◽  
T.Z. Ahkmetov ◽  
V.I. Kapralova
Keyword(s):  
Experimental Data ◽  
Protective Effect ◽  
Steel Surface ◽  
Nitrate Solution ◽  
Potassium Nitrate ◽  
Disk Electrode ◽  
Experimental Conditions ◽  
Inhibiting Action ◽  
Steel Disk ◽  
Potassium Nitrate Solution

<p>Inhibition properties of a number of glass-like polyphosphates on 65J steel were investigated using the gravimetry and methods potassium nitrate solution polarization curves in a 0,1M (pH=6,05). The possibilities<br />of optimum experimental conditions were considered as well. Basing on the experimental data the inhibition coefficient and protective effect were found. The inhibiting action of polyphosphates, apparently, is due to the formation of inhomogeneous protecting film on the steel surface.</p>

scholarly journals Protective effect of guarana-loaded liposomes on hemolytic activity

2020 ◽  
Vol 187 ◽  
pp. 110636 ◽  
Author(s):  
Isabel Roggia ◽  
Ana Júlia F. Dalcin ◽  
Aline F. Ourique ◽  
Ivana B.M. da Cruz ◽  
Euler E. Ribeiro ◽  
...  
Keyword(s):  
Protective Effect ◽  
Hemolytic Activity

171 Protective Effect of Sodium Pyruvate Against H2O2-Induced Oxidative Stress in Bovine and Swine Oocytes

2018 ◽  
Vol 30 (1) ◽  
pp. 225
Author(s):  
I. Daverio ◽  
M. Barandalla ◽  
C. Galli ◽  
G. Lazzari
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Embryonic Stem ◽  
Control Group ◽  
Minor Effect ◽  
Sodium Pyruvate ◽  
Experimental Conditions ◽  
H2o2 Treatment ◽  
Maturation Rate ◽  
The Difference

A previous study from our laboratory (Ramos-Ibeas et al. 2017 Mol. Cell. Biochem. 429, 137-150, 10.1007/s11010-017-2942-z) described the protective effect of sodium pyruvate (SP) against H2O2-induced oxidative stress in somatic and embryonic stem cells. In this study, we tested the same experimental conditions on bovine and swine oocytes during in vitro maturation (IVM). To this aim, cumulus-enclosed oocytes (COC) recovered from bovine and swine ovaries were treated for 1 h with different concentrations of H2O2 and then matured in serum-free TCM-199 with and without SP supplementation. All experiments were done in 3 replicates using 12 to 20 oocytes per experimental group; data were analysed by two-way ANOVA. As a first step, we determined the dose-response curve of H2O2 treatment during IVM, using the reaching of metaphase 2 as endpoint. We found that swine COC were more sensitive to H2O2 damage than bovine COC: indeed, they tolerated a range of concentrations from 128 to 512 μM, whereas the range for bovine COC was between 1024 and 4096 μM. The COC from both species were treated for 1 h with 128 μM (swine) or 2048 μM (bovine) H2O2 and then matured with or without different concentrations of SP. Control COC, unexposed to H2O2, were also matured with and without SP supplementation. We found that the addition of 25 mM SP to swine COC reduced the maturation rate both in control and H2O2-treated groups. Instead, reducing the addition of SP to 10 mM we observed a beneficial effect because the metaphase II level in treated oocytes increased from 38% (15/40) to 67% (24/36) and the difference was statistically significant. In bovine COC, the addition of 25 mM SP did not affect control oocytes during IVM but decreased maturation rate in treated oocytes, whereas 10 mM SP had no effect on the control group and showed a tendency to increase maturation rate in treated oocytes. In a second set of experiments, to determine if cumulus cells acted as a barrier for SP to reach the oocyte, the COC were denuded after H2O2 treatment and matured with or without SP. In swine denuded oocytes (DO) matured with SP, the rate of metaphase II increased significantly both in control [from 71% (34/48) to 92% (44/48)] and in treated groups [from 48% (23/48) to 72% (36/50)]. In bovine DO, the supplementation of SP did not increase the maturation rate significantly (25/41 v. 29/41 in control groups, 12/36 v. 18/40 in treated groups). In conclusion, we found that toxic H2O2 concentrations were approximately 10-fold higher for bovine than for swine, indicating that bovine COC were more resistant to oxidative stress. This could explain why SP supplementation had a significant protective effect in swine but only a minor effect in bovine. Moreover, the difference in SP effect between bovine and swine COC could be due to their morphology because bovine cumulus is often larger and more compact than swine cumulus and this difference could influence how SP reaches the inner oocyte and how it is metabolized. This work was funded by projects EpiHealth FP7 n. 278418, EpiHealthNet FP7-PEOPLE-2012-ITN n. 317146 and Fecund FP7 n. 312097.

Differential effects of furosemide on porcine bronchial arterial and airway smooth muscle

2000 ◽  
Vol 89 (4) ◽  
pp. 1360-1364 ◽  
Author(s):  
Michel R. Corboz ◽  
Stephen T. Ballard ◽  
Hong Gao ◽  
Joseph N. Benoit ◽  
Sarah K. Inglis ◽  
...  
Keyword(s):  
Blood Flow ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Protective Effect ◽  
Airway Obstruction ◽  
Airway Smooth Muscle ◽  
Bronchial Smooth Muscle ◽  
Experimental Conditions ◽  
Bronchial Arteries ◽  
Young Pigs

Furosemide attenuates airway obstruction in asthmatic subjects when administered as an aerosol pretreatment. This protective effect of furosemide could be related to relaxation of bronchial smooth muscle or to increased bronchial blood flow. To determine whether furosemide dilates bronchial smooth muscle, isometric contractile responses in distal bronchi from young pigs were studied. In bronchial smooth muscle rings that were precontracted with 10−5 M acetylcholine, significant relaxation occurred with 10−8 to 3 × 10−6 M isoproterenol but not with 10−8 to 10−3 M furosemide. In contrast, bronchial arteries that were precontracted with either 10−4 M norepinephrine or 10−8 M vasopressin significantly relaxed in response to 10−4 to 3 × 10−3 M and 10−3 to 3 × 10−3 M furosemide, respectively. We conclude that furosemide, under the described experimental conditions, relaxes airway vascular smooth muscle but not bronchial smooth muscle. These results are consistent with previous suggestions that inhaled furosemide increases blood flow to airway tissues (Gilbert IA, Lenner KA, Nelson JA, Wolin AD, and Fouke JM. J Appl Physiol 76: 409–415, 1994).

Accroissement par Staphylococcus aureus de la résistance de la souris envers l'infection par Pseudomonas aeruginosa

1976 ◽  
Vol 22 (7) ◽  
pp. 1024-1033
Author(s):  
J. deRepentigny ◽  
L. G. Mathieu ◽  
J. Coallier-Ascah
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Protective Effect ◽  
Peritoneal Cavity ◽  
Opportunistic Pathogens ◽  
Experimental Conditions ◽  
Sublethal Doses ◽  
Resistance To Infection ◽  
Lethal Doses

In our study of opportunistic pathogens, we have some indication that Staphylococcus aureus can increase resistance in mice against Pseudomonas aeruginosa. Intraperitoneal injections of sublethal doses of S. aureus had a protective effect in mice against lethal doses of P. aeruginosa, more so if living and coagulase-positive S. aureus strains were injected. This protective effect was obtained both with laboratory and freshly isolated hospital strains. The interval between these infections can be extended from 2 h up to 1 week and it is still possible to observe the resistance phenomenon. The increased resistance was accompanied by a decrease in viable units of P. aeruginosa in the peritoneal cavity of mice 6 h after the injection of this species. There was no protection by S. aureus against Candida albicans in similar experimental conditions. These observations indicate that intermicrobial ecology, understood here as the previous presence of another species in a host, may be a significant factor in the resistance to infection with opportunistic pathogens such as P. aeruginosa.

Protective effect of energy metabolism regulators in alteration of gravitation load under experimental conditions

2006 ◽  
Vol 142 (4) ◽  
pp. 441-446 ◽  
Author(s):  
N. K. Mazina ◽  
I. M. Larina ◽  
V. A. Khazanov ◽  
B. S. Shenkman ◽  
P. I. Tsapok ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Protective Effect ◽  
Experimental Conditions

scholarly journals Regulation of Na+,K+-ATPase and the Na+/K+/Cl+ co-transporter in the renal epithelial cell line NBL-1 under osmotic stress

1996 ◽  
Vol 319 (2) ◽  
pp. 337-342 ◽  
Author(s):  
Andreu FERRER-MARTINEZ ◽  
F. Javier CASADO ◽  
Antonio FELIPE ◽  
Marçal PASTOR-ANGLADA
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Renal Epithelial Cell ◽  
Intact Cells ◽  
Absolute Increase ◽  
Hypertonic Medium ◽  
Renal Epithelial Cell Line ◽  
Hypertonic Shock ◽  
Α1 Subunit

The long-term adaptation of the Na+,K+-ATPase to hypertonicity was studied using the bovine renal epithelial cell line NBL-1. Na+,K+-ATPase activity measured in intact cells as the ouabain-sensitive fraction of Rb+ uptake was stimulated (40% above controls) after incubating the cells in hypertonic medium. This stimulation was not correlated with significant changes in the amount of Na+,K+-ATPase α1 subunit protein. Nevertheless, the amount of α1 but not β1 subunit mRNA progressively increased after hypertonic shock (3–4-fold above basal values). These results suggest that the α1 subunit gene is modulated by medium osmolarity, although this does not necessarily involve enhanced translation of the mRNA into active α1 protein. Indeed, the increase in the biological activity of the Na+,K+-ATPase is abolished when the electrochemical Na+ transmembrane gradient is depleted by monensin, which is consistent with a post-translational effect on the activity of the sodium pump. A furosemide-sensitive component of Rb+ uptake, attributable to Na+/K+/Cl- co-transporter activity, was very low when cells were cultured in a regular medium, but was greatly induced after hypertonic shock. This induction could not be blocked by cycloheximide. Colcemide addition slightly reduced the absolute increase in Na+/K+/Cl- co-transporter activity, while cytochalasin B significantly potentiated the effect triggered by hypertonic shock. It is concluded: (i) that in NBL-1 cells the α1 but not the β1 subunit of the Na+,K+-ATPase is encoded by an osmotically sensitive gene, and (ii) that the Na+/K+/Cl- co-transporter, although an osmotically sensitive carrier, is induced by a mechanism that is independent of protein synthesis but may rely, in an undetermined manner, on the structure of the cytoskeletal network.

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