COMPARATIVE ANALYSIS OF IMPACT OF TUMOUR ANTIGEN PREPARATION METHODS ON HUMAN DENDRITIC CELLS PRIMING AND EFFICIENT CYTOKINE-INDUCED KILLER CELLS ACTIVATION IN VITRO

2021 ◽  
Vol 86 (1) ◽  
Author(s):  
M. S. Zhunussova ◽  
A. S. Issabekova ◽  
V. B. Ogay
Keyword(s):  
Dendritic Cells ◽  
Comparative Analysis ◽  
Tumour Antigen ◽  
Killer Cells ◽  
Preparation Methods ◽  
Antigen Preparation ◽  
Human Dendritic Cells

scholarly journals Effects of Staphylococcus aureus Bacteriophage K on Expression of Cytokines and Activation Markers by Human Dendritic Cells In Vitro

Viruses ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 617 ◽  
Author(s):  
Helen Freyberger ◽  
Yunxiu He ◽  
Amanda Roth ◽  
Mikeljon Nikolich ◽  
Andrey Filippov
Keyword(s):  
Staphylococcus Aureus ◽  
Dendritic Cells ◽  
Inflammatory Response ◽  
Adaptive Immune Response ◽  
Human Monocyte ◽  
Activation Markers ◽  
Mhc I ◽  
Adaptive Immune ◽  
Human Dendritic Cells

A potential concern with bacteriophage (phage) therapeutics is a host-versus-phage response in which the immune system may neutralize or destroy phage particles and thus impair therapeutic efficacy, or a strong inflammatory response to repeated phage exposure might endanger the patient. Current literature is discrepant with regard to the nature and magnitude of innate and adaptive immune response to phages. The purpose of this work was to study the potential effects of Staphylococcus aureus phage K on the activation of human monocyte-derived dendritic cells. Since phage K acquired from ATCC was isolated around 90 years ago, we first tested its activity against a panel of 36 diverse S. aureus clinical isolates from military patients and found that it was lytic against 30/36 (83%) of strains. Human monocyte-derived dendritic cells were used to test for an in vitro phage-specific inflammatory response. Repeated experiments demonstrated that phage K had little impact on the expression of pro- and anti-inflammatory cytokines, or on MHC-I/II and CD80/CD86 protein expression. Given that dendritic cells are potent antigen-presenting cells and messengers between the innate and the adaptive immune systems, our results suggest that phage K does not independently affect cellular immunity or has a very limited impact on it.

Regulatory perspective on in vitro potency assays for human dendritic cells used in anti-tumor immunotherapy

Cytotherapy ◽  
2018 ◽  
Vol 20 (11) ◽  
pp. 1289-1308 ◽  
Author(s):  
CHARLOTTE DE Wolf ◽  
MARJA VAN DE BOVENKAMP ◽  
MARCEL HOEFNAGEL
Keyword(s):  
Dendritic Cells ◽  
Tumor Immunotherapy ◽  
Regulatory Perspective ◽  
Human Dendritic Cells

The novel adjuvant CoVaccineHT™ increases the immunogenicity of cell-culture derived influenza A/H5N1 vaccine and induces the maturation of murine and human dendritic cells in vitro

Vaccine ◽  
2009 ◽  
Vol 27 (49) ◽  
pp. 6833-6839 ◽  
Author(s):  
R. Bodewes ◽  
M.M. Geelhoed-Mieras ◽  
J.G.M. Heldens ◽  
J. Glover ◽  
B.N. Lambrecht ◽  
...  
Keyword(s):  
Cell Culture ◽  
Dendritic Cells ◽  
Influenza A ◽  
The Novel ◽  
Human Dendritic Cells ◽  
H5n1 Vaccine

Comparative Analysis of Transduced Primary Human Dendritic Cells Generated by the Use of Three Different Lentiviral Vector Systems

2010 ◽  
Vol 47 (3) ◽  
pp. 262-269 ◽  
Author(s):  
Elena Grabski ◽  
Zoe Waibler ◽  
Silke Schüle ◽  
Björn-Philipp Kloke ◽  
Linda Y. Sender ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Comparative Analysis ◽  
Lentiviral Vector ◽  
Vector Systems ◽  
Human Dendritic Cells

DC-SIGN enhances infection of cells with glycosylated West Nile virus in vitro and virus replication in human dendritic cells induces production of IFN-α and TNF-α

2008 ◽  
Vol 135 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Byron E.E. Martina ◽  
Penelopie Koraka ◽  
Petra van den Doel ◽  
Guus F. Rimmelzwaan ◽  
Bart L. Haagmans ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
West Nile Virus ◽  
Virus Replication ◽  
West Nile ◽  
Tnf Α ◽  
Human Dendritic Cells

Expression of Surface Antigens During the Differentiation of Human Dendritic Cells vs Macrophages from Blood Monocytes in vitro

Immunobiology ◽  
1999 ◽  
Vol 200 (2) ◽  
pp. 187-204 ◽  
Author(s):  
Alessandro D. Santin ◽  
Paul L. Hermonat ◽  
Maurizio Chiriva-Internat Ravaggi ◽  
Maurizio Chiriva-Internat ◽  
Martin J. Cannon ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Surface Antigens ◽  
Blood Monocytes ◽  
Human Dendritic Cells

scholarly journals Fimbriated Porphyromonas gingivalis Is More Efficient than Fimbria-Deficient P. gingivalis in Entering Human Dendritic Cells In Vitro and Induces an Inflammatory Th1 Effector Response

2004 ◽  
Vol 72 (3) ◽  
pp. 1725-1732 ◽  
Author(s):  
Ravi Jotwani ◽  
Christopher W. Cutler
Keyword(s):  
Dendritic Cells ◽  
Porphyromonas Gingivalis ◽  
Cell Metabolism ◽  
Necrosis Factor Alpha ◽  
Immature Dendritic Cells ◽  
Factor Alpha ◽  
Ifn Γ ◽  
Gingival Mucosa ◽  
Human Dendritic Cells

ABSTRACT Porphyromonas gingivalis is a fimbriated mucosal pathogen implicated in chronic periodontitis (CP). The fimbriae are required for invasion of the gingival mucosa and for induction of CP in animal models of periodontitis. CP is associated with infection of immature dendritic cells (DCs) by P. gingivalis in situ and with increased numbers of dermal DCs (DDCs) and mature DCs in the lamina propria. The role of fimbriae in gaining entry into human DCs and how this modulates the inflammatory and effector immune responses, however, have not been explored. To address this, we generated monocyte-derived DCs (MDDCs) in vitro which phenotypically and functionally resemble DDCs. We show here that virulent fimbriated P. gingivalis 381, in contrast to its fimbria-deficient mutant, P. gingivalis DPG3, efficiently gains entry to MDDCs in a manner dependent on active cell metabolism and cytoskeletal rearrangement. In addition, uptake of 381, unlike DPG3, induces DCs to undergo maturation, upregulate costimulatory molecules, and secrete inflammation cytokines interleukin-1β (IL-1β), IL-6, tumor necrosis factor alpha, IL-10, and IL-12. Moreover, MDDCs pulsed with 381 also stimulated a higher autologous mixed lymphocyte reaction and induced a Th1-type response, with gamma interferon (IFN-γ) being the main cytokine. Monocytes used as controls demonstrated fimbria-dependent uptake of 381 as well but produced low levels of inflammatory cytokines compared to MDDCs. When MDDCs were pulsed with recombinant fimbrillin of P. gingivalis (10 μg/ml), maturation of MDDCs was also induced; moreover, matured MDDCs induced proliferation of autologous CD4+ T cells and release of IFN-γ. Thus, these results establish the significance of P. gingivalis fimbriae in the uptake of P. gingivalis by MDDCs and in induction of immunostimulatory Th1 responses.

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