scholarly journals Age features of morphological changes of the hematotesticular barrier in experimental diabetes mellitus.

Morphologia ◽  
2021 ◽  
Vol 15 (1) ◽  
pp. 60-66
Author(s):  
A-I.V. Kondrat ◽  
O.Ya. Zhurakivska
Keyword(s):  
Diabetes Mellitus ◽  
Morphological Changes ◽  
Active Oxygen Species ◽  
Glycosylated Hemoglobin ◽  
Diabetic Microangiopathy ◽  
Control Group ◽  
Statistical Research ◽  
Citrate Buffer ◽  
Ultrastructural Studies ◽  
Hematotesticular Barrier

Background. Diabetes mellitus is an acute medical and social problem in all parts of the world due to the constant increase in the incidence of the disease, severe complications, disability and high mortality. Hyperglycemia leads to oxidative stress and increased processes of formation of active oxygen species, which in turn make a significant contribution to the development of male infertility. Objective. Therefore, the aim of our study was to establish morphological changes in the vessels of the hemomicrocirculatory flow and testicular sustentocytes of adult rats with experimental streptozotocin diabetes mellitus (SDM). Methods. The material for the study were the testicles of 20 sexually mature 6-month-old rats (weighing 150-180 g). SDM in animals of the experimental group was simulated by a single intraperitoneal injection of streptozotocin (dissolved in 0.1 M citrate buffer solution with a pH of 4.5) at a dose of 6 mg per 100 g of mass. An equivalent dose of 0.1 M citrate buffer was injected intraperitoneally to animals of the control group. Histological, electron microscopic, biochemical, morphometric and statistical research methods were used. Results. It was found that in the early stages of SDM (28th day) on the background of hyperglycemia in the hemomicrocirculatory flow of the testes there is a spasm of the vessels of the afferent link, which is confirmed by a decrease in the lumen of arterioles and an increase in their Wagenworth index. On the 70th day of SDM on the background of elevated levels of glucose and glycosylated hemoglobin in the links of the hemomicrocirculatory flow of the testis there are signs of diabetic microangiopathy, manifested by: hemorheological disorders in micro-hemo-vessels, (erythrocyte sludge, adhesion of erythrocytes and platelets, microclasmatosis), decrease in the capacity of arterioles and capillaries (increase in the Wagenworth index, respectively by 1.8 and 1.9 times), microclasmatosis, thickening and proliferation of the basement membrane of capillaries. Against the background of diabetic microangiopathy, there is a decrease in the number of sustentocytes by 0.01 mm2 of testicular parenchyma by 1.8 times, compared with control indicators, the area of their profile increases by 2.2 times (in all cases p<0.05). The nucleolar areas probably do not change, which leads to an increase in the nuclear-cytoplasmic ratio by 2.9 times (p<0.05). Such morphometric changes of sustentocytes are caused by development of vacuolar hydropic dystrophies in them, and an apoptosis that is confirmed by data of histo-ultrastructural studies. Changes in sustentocytes against the background of the development of diabetic microangiopathy lead to a violation of the hematotesticular barrier and to dystrophic changes in the spermatogenic epithelium of the testis. Conclusion. Thus, on the 70th day of SDM in the hemomicrocirculatory flow of the testis, the development of diabetic microangiopathy is observed, which leads to a violation of the hematotesticular barrier, and as a consequence, to a violation of spermatogenesis.

scholarly journals Age features of morphological changes of vessels of testicular hemomicrocirculatory flow in streptozotocin diabetes mellitus

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Ivan-Andriy Kondrat
Keyword(s):  
Diabetes Mellitus ◽  
Morphological Changes ◽  
Glycosylated Hemoglobin ◽  
Streptozotocin Diabetes ◽  
Male Rats ◽  
Diabetic Microangiopathy ◽  
Control Group ◽  
Buffer Solution ◽  
Statistical Research ◽  
Citrate Buffer

Medico-social problem of diabetes is caused by early disability and mortality of patients due to specific complications of micro- and macroangiopathies. Therefore, the aim of our study was to establish morphological changes in vessels of the hemomicrocirculatory flow of the testes of immature rats with experimental streptozotocin diabetes mellitus (SDM). The material for the study were the testicles of 20 two-month-old immature (weighing 65-95 g) white outbred male rats, which were divided equally into 2 groups: experimental and control ones. SDM in animals of the experimental group was simulated by a single intraperitoneal injection of streptozotocin (dissolved in 0.1 M citrate buffer solution with a pH of 4.5) at a dose of 7 mg per 100 g of mass. The control group of animals received intraperitoneally an equivalent dose of 0.1 M citrate buffer. Histological, electron microscopic, biochemical, morphometric and statistical research methods were used. It was found that in the early stages of SDM (14th day) on the background of hyperglycemia in the hemomicrocirculatory flow of the testes there is a spasm of the vessels of the afferent link, which is confirmed by a decrease in the area of arterioles lumen and an increase in their VI. On the 56th day of SDM, on the background of elevated levels of glucose and glycosylated hemoglobin in the links of the hemorrhagic circulatory flow of the testes there are initial signs of diabetic microangiopathy, manifested by: hemorheological disorders in micro-hemo-vessels (erythrocyte sludges, adhesion of erythrocytes and platelets, microclasmatosis), decreased capacity of arterioles and capillaries (increase in VI, respectively by 1.2 and 1.9 times), microclasmatosis, thickening and proliferation of the basement membrane of capillaries. Thus, on the 56th day of SDM in the hemomicrocirculatory flow of the testes, the development of diabetic microangiopathy is observed, which leads to the disruption of the blood-testis barrier, and as a consequence, to a violation of spermatogenesis.

scholarly journals Fenofibrate decreased microalbuminuria in the type 2 diabetes patients with hypertriglyceridemia

2020 ◽  
Author(s):  
Xiaomeng Sun ◽  
Jia Liu ◽  
Guang Wang
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Treatment Group ◽  
Glycosylated Hemoglobin ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Diabetic Patients ◽  
Fenofibrate Treatment

Abstract Background: This study was to research the efficacy of fenofibrate in the treatment of microalbuminuria in the patients with type 2 diabetes mellitus (T2DM) and hypertriglyceridemia. Methods: Type 2 diabetic patients (56) with microalbuminuria and hypertriglyceridemia aged 30 to 75 were randomly divided into the fenofibrate treatment group(n=28) and the control group (n=28) for 180 days. Urinary microalbumin /creatinine ratio (UACR) and other metabolic parameters were compared at baseline, during treatment and after treatment. Results: After 180 days, the reduction of levels of fasting blood glucose (FBG) and glycosylated hemoglobin (HbA1c) in two groups were no differences. In treatment group, uric acid (UA) (296.42 ± 56.41 vs 372.46 ± 72.78), triglyceride (TG) [1.51(1.17, 2.06) vs 3.04(2.21, 3.29)], and UACR [36.45 (15.78,102.41) vs 129.00 (53.00, 226.25)] were significantly decreased compared with the baseline. The high-density lipoprotein cholesterol (HDL-C) levels were significantly increased (1.22 ± 0.26 vs 1.09 ± 0.24) compared with the baseline. The decrease in UACR [-44.05(-179.47, -12.16) vs -8.15(-59.69, 41.94)]in treatment group was significantly higher compared with the control group. The decrease in UACR was positively associated with the decreases in TG ( r = 0.447, P = 0.042) and UA ( r = 0.478, P = 0.024) after fenofibrate treatment. Conclusion: In the patients with hypertriglyceridemia and type 2 diabetes mellitus, fenofibrate can improve microalbuminuria and do not increase the deterioration of glomerular filtration rate

scholarly journals SUPERFICIAL CANDIDOSIS COURSE IN PATIENTS WITH CARBOHYDRATE METABOLISM DISORDER AND DIABETES MELLITUS

2019 ◽  
Vol 72 (1) ◽  
pp. 68-71
Author(s):  
Оrysya О. Syzon ◽  
Solomiya A. Turkevych ◽  
Tetyana I. Rudnyk ◽  
Svitlana V. Volbyn ◽  
Marianna O. Dashko
Keyword(s):  
Diabetes Mellitus ◽  
Carbohydrate Metabolism ◽  
Glucose Tolerance Test ◽  
Glycosylated Hemoglobin ◽  
Tolerance Test ◽  
Control Group ◽  
Clinical Tests ◽  
Metabolism Disorder ◽  
Mucous Membranes ◽  
Reliable Detection

Introduction: One of the pressing issues of modern dermatology is the prevalence of diseases of skin and mucous membranes caused by yeast-like fungi. The aim: To study specific features of the course of skin and mucous membranes superficial candidosis against the background of initial carbohydrate metabolism disorders or diabetes mellitus. Materials and methods: We have examined 93 patients aged 18-75 suffering from superficial candidosis. Candidosis was diagnosed based on anamnesis data, results of clinical tests and laboratory studies. The study of carbohydrate metabolism included determining of the glucose level under fasting conditions to establish possible diabetes mellitus and glucose tolerance test (75 g glucose load), glycosylated hemoglobin (HbA1c) and fructosamine (FR). Results: In patients with mild and moderate candidosis we have detected an increased rates of НbA1с and fructosamine, as compared with the like indicators received in the control group, by 1.54 and 1.21 times respectively. In patients with severe candidosis and relapses the concentrations of НbA1с increased by 2.59 times, FR – by 2.26 times against the indicators received in the control group. Thus, increased levels of blood glycosylated protein indicate the pathogenetic importance of these processes in the development of candidosis. Conclusions: Consequently, increased levels of blood glycosylated protein indicates the pathogenetic importance of these processes in the development of candidal lesions. Tests for НbA1с and FR proved to be sensitive and allow reliable detection of individuals with diabetes mellitus and glucose intolerance among candidosis patients.

scholarly journals Diagnostic capabilities of different methods of laser doppler flowmetry spectral indexes assessment in patients with diabetic microangiopathy

2021 ◽  
Vol 10 (2) ◽  
pp. 18-24
Author(s):  
P. V. Vasilev ◽  
N. P. Erofeev ◽  
A. N. Shishkin
Keyword(s):  
Diabetes Mellitus ◽  
Laser Doppler Flowmetry ◽  
Main Group ◽  
Laser Doppler ◽  
Diabetic Microangiopathy ◽  
Control Group ◽  
Spectral Parameters ◽  
Doppler Flowmetry ◽  
Diagnostic Capabilities ◽  
And Control

The article contains the results of a study of two different methods for calculating the spectral parameters of laser Doppler flowmetry in patients with diabetic microangiopathy caused by type 2 diabetes mellitus (main group) and those with excluded diabetes mellitus (control group). Spectral indices were calculated using either average or maximum amplitudes of the frequency ranges. When comparing the contribution of respiratory and pulse fluxmotions using average amplitudes, there were significant (p < 0.05) differences between the main and control groups. On the contrary, when using the maximum amplitudes, no significant differences were noted (p > 0.05). Also, significant correlations were found between the contributions of respiratory and pulse fluxmotions and the estimated glomerular filtration rate in the main group, using both calculation methods. These studies indicate the feasibility of using a technique based on the analysis of average amplitudes to increase the specificity of laser Doppler flowmetry as a method for diagnosing diabetic microangiopathy.

scholarly journals Effect of long-term hyperglycemia on cornea and retina morphology in rats with streptozotocin-induced diabetes mellitus

2021 ◽  
pp. 42-47
Author(s):  
А.А. Панов ◽  
Е.М. Ржавина ◽  
М.П. Морозова ◽  
А.К. Ердяков ◽  
С.А. Гаврилова
Keyword(s):  
Diabetes Mellitus ◽  
Ketone Bodies ◽  
Morphological Changes ◽  
Posterior Part ◽  
Male Rats ◽  
Control Group ◽  
Blood Concentrations ◽  
Central Retina ◽  
Streptozotocin Induced Diabetes

Цель исследования - изучение динамики морфологических изменений роговицы и заднего отдела глаза крыс при длительной гипергликемии. Методика. Исследование выполнено на 36 самцах крыс Wistar. Сахарный диабет индуцировали внутрибрюшинной инъекцией стрептозотоцина (65 мг/кг), после чего ежедневно вводили подкожно малые дозы инсулина (2 ЕД/кг). На 50-е, 58-е и 66-е сут эксперимента производили энуклеацию глаз у глубоко наркотизированных животных. Гистологические срезы фрагментов глаз окрашивали гематоксилин-эозином, проводили морфометрию параметров роговицы и сетчатки. Результаты. Средняя концентрация глюкозы и кетоновых тел крови в группе сахарного диабета составила 29,8 ммоль/л и 0,889 ммоль/л, в контрольной группе - 6,2 ммоль/л и 0,847 ммоль/л соответственно. Анализ гистологических срезов глаз выявил признаки отека роговицы, хориоидеи и наружных слоев центральных отделов сетчатки до появления других качественных и количественных морфологических изменений. Заключение. Оценка толщины роговицы, хориоидеи и наружных слоев центральных отделов сетчатки может служить предиктором развития диабетической ретинопатии. The aim was to study morphological changes in the cornea and the posterior part of rat eye during prolonged hyperglycemia. Methods. The study was performed on 36 Wistar male rats. Diabetes mellitus was induced by an injection of streptozotocin (65 mg/kg, i.p.) followed by daily injections of low doses of insulin (2 U/kg, s.c.). Eyes were enucleated from deeply anesthetized rats on days 50, 58, and 66 of the experiment. Histologic sections were stained with hematoxylin-eosin, and morphometry of the cornea and the retina was performed. Results. Mean blood concentrations of glucose and ketone bodies were 29.8 mmol/L and 0.889 mmol/L, respectively, in the diabetic group and 6.2 mmol/L and 0.847 mmol/L, respectively, in the control group. The histological analysis revealed signs of edema in the cornea, choroid and outer layers of the central retina, which preceded other morphological changes. Conclusion. Evaluating thickness of the cornea, choroid and outer layers of the central retina may serve for prediction of diabetic retinopathy.

New approaches to early diagnostic of microangiopathy in patients with type 1 diabetes mellitus

2015 ◽  
Vol 61 (5) ◽  
pp. 43-47
Author(s):  
Evgeniy Sergeevich Krutikov ◽  
Viktoriya Andreevna Zhitova
Keyword(s):  
Diabetes Mellitus ◽  
Type 1 Diabetes ◽  
Recovery Time ◽  
Structural Changes ◽  
Diabetic Microangiopathy ◽  
Control Group ◽  
Functional Disorders ◽  
Functional Tests ◽  
Early Diagnostic

Aim — the development of method for early diagnosis of microangiopathy in patients with type 1 diabetes mellitus (DM) by capillaroscopy with functional tests: the cuff occlusion of the upper extremity, tests with the cooling and heat exposures on the upper extremity.Material and methods. We investigated 54 patients with DM type I (mean age 24,1±3.2 years), who were divided into 2 groups: I group — 28 (51.6%) patients with duration DM <5 years; group II — 26 (48.4%) with duration DM >7 years. All the patients were underwent complex laboratory and instrumental examinations and also capillaroscopy and functional tests. Control group was consisting of 26 healthy people.Results. We revealed early stages of the diabetic microangiopathy even before the appearance of structural changes in patients with DM type at functional tests. We were identified the functional disorders: increasing recovery time of capillaroscopic parameters after carrying out functional tests to baseline versus control.Conclusion. We were to diagnose structural changes of microcirculation in diabetic patients with disease duration more than 7 years by capillaroscopy. In the early stages of the disease we were observed functional disorders which appearance before the structural changes using functional tests. It was lengthened recovery time of capillaroscopic dates after carrying out functional tests to baseline values in comparison with the control group. Recovery time may be used as early diagnostic criteria of diabetic microangiopathy when carrying out functional tests in patients with DM.

scholarly journals Morphology of bronchial epithelium in rodent streptozotocin-induced diabetes mellitus

2013 ◽  
Vol 16 (4) ◽  
pp. 44-48
Author(s):  
Oksana Anatolyevna Pivovarova ◽  
Boris Nikitich Mankovsky
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Wistar Rats ◽  
Bronchial Epithelium ◽  
Secretory Cells ◽  
Control Group ◽  
Partial Loss ◽  
Citrate Buffer ◽  
Single Intraperitoneal Injection ◽  
Streptozotocin Induced Diabetes

Aim.  To study the morphology of bronchial epithelium in a rodent streptozotocin-induced (STZ) diabetes mellitus. Materials and Methods. . Diabetes mellitus was introduced in 47 white Wistar rats aged 5?6 months (body weight 234.0?2.64 g). 43 white Wistar rats of the same age were used as control subjects (body weight 242.0?2.13). Diabetes was induced by single intraperitoneal injection of STZ (SIGMA, USA) 60 mg/kg in 0.1 M citrate buffer, pH 4.5. Results. A statistically significant decrease in the total epithelial area by 25.9% was observed in the study group, accompanied by a reduction of the supranuclear zone by 22.1% vs. the control group. Conclusion. We found that bronchial mucous membrane in rodents with STZ-induced diabetes mellitus exhibits signs of atrophy and partial loss of mucous production by bronchial secretory cells.

scholarly journals APOPTOTIC PROCESSES’ RESEARCH ON THE MODEL OF TYPE 2 DIABETES MELLITUS ON OBESITY’S BACKGROUND UNDER THE INFLUENCE OF DENSE BEAN EXTRACT

2020 ◽  
Vol 73 (8) ◽  
pp. 1690-1695
Author(s):  
Viktoria A. Rybak ◽  
Viktoria V. Korol ◽  
Natalia V. Derkach
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Cell Function ◽  
Male Rats ◽  
Control Group ◽  
Citrate Buffer ◽  
Pancreatic Cell ◽  
Alcoholic Fatty Liver

The aim of the study was to observe the influence of dense bean extract on the intensity of apoptotic processes in the liver cells and pancreas of rats on a model of type 2 diabetes mellitus on obesity’s background. Materials and methods: The main method was to model type 2 diabetes mellitus on the background of obesity in organism of mature six-month-old male rats of the Wistar population (n = 21), weighing 150-170 g. The modelling was carried out by intraperitoneal low dose administration of streptozotocin (30 mg / kg, in citrate buffer pH = 4, 5) inside after three months period of keeping animals on a combined diet. Apoptosis in DNA samples of liver and pancreas cells was identified in duplicates using electrophoresis in a 1% agarose gel with using a 1kb DNA SibEnzyme apoptosis marker (from 10,000 to 250 nucleotides). Results: Only in two of the seven studied DNA samples of the pancreas of a group of rats, treated with a dense bean extract, were observed the traces of necrosis without detectable manifestations of the apoptotic process. It situates at the level of indicators of the animals’ intact control group and indicates the distinct effect’s presence which includes maintaining pancreas cells survival (in both endocrine and exocrine parts) if imbalance of carbohydrate and lipid metabolism take place in organism. Conclusion: Dense bean extract showed a more distinct effect than the comparison drug metformin in relation to the risk of premature loss of pancreatic cell function and the development of non-alcoholic fatty liver disease. A dense bean extract is promising for further pharmacological studies, with the aim of creating phytopreparations – «Glyphasonorm» tablets and «Glyfasolin» capsules for the correction of type 2 diabetes mellitus and its complications.

scholarly journals Deteriorated gene expression of selected calcium transporters in streptozotocin-induced diabetic hearts of Wistar rats

2018 ◽  
Vol 65 (2) ◽  
pp. 1-3
Author(s):  
M. Dragún ◽  
G. Dóka ◽  
M. Máťuš ◽  
P. Křenek ◽  
J. Klimas
Keyword(s):  
Diabetes Mellitus ◽  
Calcium Homeostasis ◽  
Wistar Rats ◽  
Control Group ◽  
Mrna Levels ◽  
Short Term ◽  
Gene Expressions ◽  
Citrate Buffer ◽  
Total N

Abstract Aim: The aim is to identify the possible changes in the expression of genes, that regulate calcium homeostasis in cardiomyocytes in diabetes mellitus. Methods: Male Wistar rats were randomized into two experimental protocols: short-term 5-days streptozotocin-induced diabetes protocol with 20 weeks old animals at the end of the protocol (total N = 20) and long-term 4-weeks protocol with 18 weeks of age at the end of the protocol (total N = 38). 50 mg/kg of streptozotocin (STZ) was administered in both protocols by a single intraperitoneal injection in 0,1M citrate buffer (pH = 4.5). Control group (CON) received only vehiculum. Gene expressions in samples of left heart ventricle were measured by RT-qPCR method. Results: The expression of SERCA2a in short-term protocol was decreased. In long-term protocol, decreased SERCA2a, TRPC4 and TRPC6 mRNA levels were observed (*p < 0.05). SERCA2a and TRPC4 mRNA levels exhibited statistical monotonic correlation in STZ-treated group in long-term protocol. Conclusions: In diabetes mellitus, the calcium homeostasis in cardiomyocytes is altered and there could be a relation between alteration of internal sarcoplasmatic stores and store-operated calcium entry.

scholarly journals Morphological Changes of Gingiva in Streptozotocin Diabetic Rats

2009 ◽  
Vol 2009 ◽  
pp. 1-4 ◽  
Author(s):  
C. Tesseromatis ◽  
A. Kotsiou ◽  
H. Parara ◽  
E. Vairaktaris ◽  
M. Tsamouri
Keyword(s):  
Diabetes Mellitus ◽  
Morphological Changes ◽  
Diabetic Rats ◽  
Control Group ◽  
Diabetic Patients ◽  
Histological Investigation ◽  
Bacterial Diseases ◽  
Male Wistar Rats ◽  
Streptozotocin Induced Diabetes ◽  
Routine Assay

Gingivitis and periodontitis are chronic bacterial diseases of the underlying and surrounding tooth tissues. Diabetes mellitus is responsible for tooth deprivation both by decay and periodontal disease. The streptozotocin-induced diabetes results in a diabetic status in experimental animals similar to that observed in diabetes patients. The aim of the study was to investigate the relationship between the gingival lesions and the microangiopathy changes in streptozotocin-induced diabetes mellitus. Forty male Wistar rats were divided into two groups (control and experimental). Diabetes mellitus was induced by 45 mg/kg IV streptozotocin. The histological investigation of the marginal gingival and the relevant gingival papilla showed inflammation of the lamina propria and the squamous epithelium as well as marked thickness of the arteriole in the diabetic group, but no changes were observed in the control group. The results suggested a probable application of a routine gingival histological investigation in diabetic patients in order to control the progress of disease complications. It may be concluded that histological gingival investigation can be used as a routine assay for the control of the diabetic disease and prevention of its complications.

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