scholarly journals Comparative characteristics of changes in subchondral bone of rats after antenatal glucocorticoid administration and modeling of osteoporosis

Morphologia ◽  
2021 ◽  
Vol 15 (3) ◽  
pp. 62-65
Author(s):  
O.A. Hryhorieva ◽  
P.V. Bohdanov ◽  
T.M. Matvieishyna ◽  
E.R. Skakovskiy
Keyword(s):  
Subchondral Bone ◽  
Bone Fractures ◽  
Morphological Changes ◽  
Control Group ◽  
Relative Area ◽  
Serial Sections ◽  
Mineral Density ◽  
Adult Rats ◽  
Laboratory Rats ◽  
Bone Trabeculae

Background. Osteoporosis is a progressive systemic bone disease that results in decreased bone mineral density and, as a result, increases the risk of bone fractures. Changes that occur in the subchondral bone in osteoporosis or because of hormones can cause degenerative changes in the articular cartilage that underlie osteoarthritis. Objective. The aim of the study was to identify and compare morphological changes that occur in the subchondral bone in experimental simulations of osteoporosis and in adult rats that were born from females that were exposed to glucocorticoid solution administration in the 3rd trimester of pregnancy. Methods. Tibias of 26 white mature laboratory rats were studied. In serial sections, the relative areas occupied by bone trabeculae and lacunae were calculated. Results. The relative area involving the bone trabeculae (23,2 ± 3,70%) statistically significantly decreased at 21 day in the group of animals that undergone a simulation of osteoporosis in comparison with the control group. Similar changes are observed in the group of experimental animals that were born from females that were exposed to glucocorticoid solution administration in the 3rd trimester of pregnancy. Conclusion. Thus, the results of the study demonstrate the similarity of morphological changes occurring in the subchondral bone in rats that undergone a simulation of osteoporosis and rats that were born from females that were exposed to glucocorticoid solution administration in the 3rd trimester of pregnancy.

REACTION OF CD68-POSITIVE RAT LIVER AND SPLEEN CELLS ON SILICON INTAKE WITH DRINKING WATER

2021 ◽  
pp. 34-43
Author(s):  
Evgeniia A. Grigoreva ◽  
Valentina S. Gordova ◽  
Valentina E. Sergeeva ◽  
Alina T. Smorodchenko
Keyword(s):  
Drinking Water ◽  
Cytoplasmic Membrane ◽  
Unit Area ◽  
Morphological Changes ◽  
Morphological Characteristics ◽  
Control Group ◽  
Silicon Compound ◽  
Average Cell ◽  
Laboratory Rats ◽  
Experimental Group

The article presents data on the long-term effect (nine months) of a silicon compound supplied with drinking water – nonahydrate sodium metasilicate (10 mg/l in terms of silicon), on CD68-positive macrophages in the liver and spleen of laboratory rats. Changes in the morphological characteristics of this cell population were found. There was a decrease in the average cell area (in the liver of the control group of rats, the average macrophage area was 179.23±5.94 microns2, and in the group receiving silicon with drinking water – 117.04±3.35 microns2; in the spleen-136.02±3.93 microns2 and 103.44±2.8 microns2, respectively). Macrophages in the liver preparations of the experimental group of rats had a fewer processes and a darker cytoplasmic membrane. The number of macrophages in the liver per unit area was comparable, for the control group of rats it was 18.78±1.24, and for the rats that received with water with the addition of silicon – 19.41±0.75 cells. CD68+ macrophages of the red splenic pulp in laboratory rats that received silicon also underwent the following morphological changes: they were located in a denser way and had fewer processes, while the number of macrophages per unit area was 73.7±2.3 for the control group, 91.6±5.0-for the experimental group, respectively. The distance between them did not change. There was a change in the intensity of CD68 expression on the surface of the cytoplasmic membrane and in the cytoplasm of liver and spleen macrophages. These changes can be interpreted as the adaptive ability of liver and spleen macrophages to silicon introduced with drinking water. Given the heterogeneity of the macrophage population in the liver and spleen, further studies using markers for different subpopulations of macrophages are needed to clarify their role in the response of tissues to silicon supplied with drinking water.

Early Alterations of Subchondral Bone in the Rat Anterior Cruciate Ligament Transection Model of Osteoarthritis

Cartilage ◽  
2019 ◽  
pp. 194760351987847 ◽  
Author(s):  
Nik Aizah ◽  
Pan Pan Chong ◽  
Tunku Kamarul
Keyword(s):  
Anterior Cruciate Ligament ◽  
Articular Cartilage ◽  
Subchondral Bone ◽  
Cruciate Ligament ◽  
Quantitative Computed Tomography ◽  
Cartilage Loss ◽  
Control Group ◽  
Mineral Density ◽  
Anterior Cruciate Ligament Transection ◽  
Anterior Cruciate

Objective Advances in research have shown that the subchondral bone plays an important role in the propagation of cartilage loss and progression of osteoarthritis (OA), but whether the subchondral bone changes precede or lead to articular cartilage loss remains debatable. In order to elucidate the subchondral bone and cartilage changes that occur in early OA, an experiment using anterior cruciate ligament transection (ACLT) induced posttraumatic OA model of the rat knee was conducted. Design Forty-two Sprague Dawley rats were divided into 2 groups: the ACLT group and the nonoperated control group. Surgery was conducted on the ACLT group, and subsequently rats from both groups were sacrificed at 1, 2, and 3 weeks postsurgery. Subchondral bone was evaluated using a high-resolution peripheral quantitative computed tomography scanner, while cartilage was histologically evaluated and scored. Results A significant reduction in the subchondral trabecular bone thickness and spacing was found as early as 1 week postsurgery in ACLT rats compared with the nonoperated control. This was subsequently followed by a reduction in bone mineral density and bone fractional volume at week 2, and finally a decrease in the trabecular number at week 3. These changes occurred together with cartilage degeneration as reflected by an increasing Mankin score over all 3 weeks. Conclusions Significant changes in subchondral bone occur very early in OA concurrent with surface articular cartilage degenerative change suggest that factors affecting bone remodeling and resorption together with cartilage matrix degradation occur very early in the disease.

scholarly journals Prediction of osteoporosis severity in patients with rheumatoid arthritis via evaluation of the fetuin-A level

2021 ◽  
pp. 60-65
Author(s):  
Yu.R. Akhverdyan ◽  
◽  
E.V. Papichev ◽  
B.V. Zavodovsky ◽  
Ju.V. Polyakova ◽  
...  
Keyword(s):  
Roc Curve ◽  
Healthy Subjects ◽  
Bone Fractures ◽  
Test System ◽  
Laboratory Evaluation ◽  
Control Group ◽  
Osteoporotic Bone ◽  
Mineral Density ◽  
Fetuin A ◽  
Severity Prediction

Aim of study. To investigate the level of dependence of osteoporosis (OP) progression severity on the level of fetuin-A (FA) in order to predict the OP progression severity in RA patients. Material and methods. We have examined a total of 110 RA patients (mean age 54.2+11.97). Th e diagnosis was established based on ACR/EULAR criteria 2010. A total of 30 conventionally healthy subjects were enrolled serving as a control group. Evaluation of bone mineral density (BMD) was performed using DXA (Lunar DPX-NT GE densitometer). The FA level was determined through indirect enzyme-linked immunoassay (Human Fetuin-A ELISA test-system). Results. Mean serum FA in healthy subjects amounted to 653.55-972.19μg/ml. We formed 2 groups of RA patients: with blood serum FA levels <653.55μg/ml (23 patients) and with normal FA values: 653.55μg/ml or higher (87 patients). A lowered FA level was revealed in 20 (86.96 %) subjects in the group of OP patients and only in 3 (13.04 %) patients with RA without OP (р<0.001). Osteoporotic bone fractures were diagnosed in 12 (13.79 %) patients in the group with normal FA levels and were absent in 75 (86.21 %) patients (р<0.001). Apart from that, RA patients with lowered FA had reliably lower mean BMD in the area of femoral neck and vertebral column as well as lower BMD total value (р<0.001). In order to determine the diagnostic accuracy of laboratory tests, the characteristic curve (ROC curve) was applied. Th e area under the ROC-curve was 0.663 with the cut-off point matching the FA level of 660.3μg/ml. Th is value corresponds with 84.72 % specifi city and 47.37 % sensitivity. Conclusion. We have revealed reliable correlations between the serum FA level in RA patients, the frequency of OP development, osteoporotic bone fractures and BMD values (p<0.001). Laboratory evaluation of FA for OP progression severity prediction for RA patients has good quality with the specifi city of 84.72 % and the sensitivity of 47.37 %.

scholarly journals THE COMPARATIVE MORPHOLOGICAL ANALYSIS OF THE NEPHROTOXICITY OF DOXORUBICIN AND NANOSTRUCTURED DOXORUBICIN IN CONDITIONS OF TRANSPLANTABLE CARCINOGENESIS

2018 ◽  
Vol 26 (2) ◽  
pp. 45-48
Author(s):  
I. N. Chairkin ◽  
N. V. Chairkina ◽  
O. N. Deryabina ◽  
V. Y. Medvezhonkov ◽  
O. V. Kalmin
Keyword(s):  
Morphological Analysis ◽  
Structural Changes ◽  
Morphological Changes ◽  
Control Group ◽  
Structural Components ◽  
Laboratory Rats ◽  
Chemotherapy Drugs ◽  
Morphological Evaluation ◽  
Comparative Morphological Analysis ◽  
Convoluted Tubules

The study was carried out on 120 mongrel white laboratory rats, to whom were transplanted the ascitiс Zaidel's hepatoma and which were divided into three equal groups. Of these, the first group of animals was a control group without treatment. In the second group, to animals doxorubicin was injected, in the third group, nanostructured doxorubicin was injected to the animals for 21 days. The aim of the study was to assess the degree of morphological changes in the tubules and glomeruli of the kidneys under the influence of nanostructured doxorubicin and doxorubicin in the condition of transplantable carcinogenesis. Given the nephrotoxicity of the chemotherapy drugs studied, the dynamics of structural changes in the tubules and glomeruli of the kidneys was studied. Conducted a comparative morphological evaluation of the changes occurring in the kidneys. The results of a morphological study of the structural components of the kidneys prove that doxorubicin and nanostructured doxorubicin have different effects on both the tubular apparatus and the glomeruli of the kidneys in the condition of transient carcinogenesis. The use of doxorubicin is accompanied by a relatively pronounced nephrotoxic effect, which is indicated by dystrophic and necrobiotic changes in the epithelium of convoluted tubules and the glomerular apparatus of the kidneys. The use of nanostructured doxorubicin is limited to moderately expressed dystrophic changes in the epithelium of the tubular apparatus of the kidneys, the glomerular apparatus remains intact at the same time. Thus, the use of the preparation of nanostructured doxorubicin for the treatment of the ascitic hepatoma of Zaidel in experimental animals has a less pronounced toxic and damaging effect on the renal parenchyma.

scholarly journals Morphological changes in the thymus of rats of reproductive period on the background of the immunosuppressant cyclophosphamide administration

2016 ◽  
Vol 97 (4) ◽  
pp. 578-585
Author(s):  
I V Bobrysheva
Keyword(s):  
Morphological Changes ◽  
Fatty Degeneration ◽  
Reproductive Period ◽  
Relative Content ◽  
Image Analyzer ◽  
Relative Area ◽  
Thymic Involution ◽  
Laboratory Rats ◽  
Functional Zones ◽  
Functional Areas

Aim. To study the features of morphological changes of the thymus gland (thymus) of reproductive period rats amid the administration of the immunosuppressant cyclophosphamide.Methods. Thymus preparations were studied using an image analyzer based on CX-41 Olympus microscope. The morphological and functional areas of the thymus were determined relative areas (as a percentage) occupied by subcapsular, inner cortical zones of the cortex and thymic lobules medulla; cells distribution density, the percentage of cellular elements: lymphoblasts, small, medium and large lymphocytes, macrophages, mitotically dividing, destructively altered cells epithelioreticulocytes.Results. A statistically significant reduction in the relative area of subcapsular and inner cortical zones of the cortex, as well as an increase in this parameter in the thymic medullary parenchyma after 1-30 days after administration of cyclophosphamide were found. The cell density is reduced both in the cortex and in the medulla. Cytoarchitectonics of the thymus morphological and functional zones changes: a decrease in relative content of lymphocytes, mainly of young forms, as well as increase in the percentage of macrophages and destructively altered cells occurred. Maximum deviations of studied parameters was recorded after 1 and 7 days after drug administration. In 60 days after cytostatic administration morphometric characteristics of the thymus morphological and functional zones in laboratory rats do not have significant differences from the parameters in control animals of appropriate age.Conclusion. A single administration of cyclophosphamide leads to acute thymic involution, which is manifested in a decrease in the relative area of the cortex, lobules deformation, organ fatty degeneration, decrease in the relative content of lymphocytes, increase in the percentage of macrophages and destructively modified cells, which may be indicative of a high degree of reactivity of the animals thymus; results of the study may serve as morphologic evidence of the damaging effect of cyclophosphamide on the thymus lymphoid tissue.

Effect of maternal and postnatal cocoa supplementation on testicles of adult Wistar rats

2020 ◽  
pp. 1-7
Author(s):  
Juliana Lima ◽  
Letícia Cardoso ◽  
Gabrielle Rocha ◽  
Caroline Fernandes-Santos ◽  
Diogo de Souza ◽  
...  
Keyword(s):  
Functional Foods ◽  
Morphological Changes ◽  
Control Group ◽  
Postnatal Life ◽  
Early Weaning ◽  
Adult Rats ◽  
Epithelial Surface ◽  
Cocoa Powder ◽  
Testicular Morphology ◽  
Tubular Diameter

Abstract Early weaning can lead to changes in the morphology of organs in adulthood, and the consumption of functional foods during lactation and postnatal life is believed to prevent these changes. However, it is not known if early weaning affects testicular morphology and if the use of cocoa can prevent that. We studied the effects of maternal and postnatal supplementation of cocoa powder on the testicular morphology of early weaned adult rats. The animals were divided into four groups (n = 6 each), control group, cocoa control group, early weaning (EW) group, and cocoa early weaning (EWCa) group, and were analyzed for 90 d, after which they were euthanized. The animals from the EW group showed a reduction in the tubular diameter and height of the seminiferous epithelium, a decrease in epithelial surface density (Sv), and an increase in the lumen and proper tunic. However, the animals from the EWCa group showed an increase in the diameter and height of the epithelium, an increase in the epithelium Sv, and a decrease in the lumen and the proper tunic. The early weaning promotes morphological changes in the testicles; however, supplementation with cocoa powder can preserve the testicular histoarchitecture.

Study of bone mineral saturation in the dynamics of therapy of professionally caused osteopathies of the shoulder joint

2019 ◽  
pp. 1009-1012
Author(s):  
V. N. Druzhinin ◽  
V. G. Suvorov ◽  
A. N. Cherny ◽  
S. N. Troynakov ◽  
U. T. Tukhtaev
Keyword(s):  
Shoulder Joint ◽  
New Technologies ◽  
Proximal Humerus ◽  
Morphological Changes ◽  
Shoulder Girdle ◽  
Radiological Examination ◽  
Control Group ◽  
Mineral Density ◽  
Objective Criterion ◽  
Digital Reference

The relevance of the study is due to the high prevalence of degenerative-dystrophic changes in the bone-articular apparatus and periarticular tissues in the structure of diseases in General in the population and in persons working in contact with harmful production factors. The most common in clinical practice diseases of the shoulder girdle with lesions of bone-cartilage and tendon-muscle structures include arthrosis and changes in periarticular tissues, including: periarthritis, tendinitis, tenosynovitis (tendovagines, entezity), enthesopathy, bursitis, ruptures of the rotator cuff of the humerus, the symptom of “collision” — impingement sholder syndrome, lesions of the acromioclavicular and clavicular joints. Treatment of these conditions is not a solved medical and social problem, so perhaps their early diagnosis today is still relevant in the sense of primary prevention of disability of the population, including the working part of it-improving the quality of diagnosis of shoulder joint pathology today can be achieved through the use of new methodological techniques, objectifying the degree of severity of x-ray morphological changes on the basis of appropriate quantitative characteristics.The aim of the study was to optimize the diagnosis of osteopenia (osteoporosis) using digital reference osteodensitometry to objectively assess the effectiveness of therapy for ostearthritis and periarthritis of the shoulder joint.A comparative analysis of the results of clinical and radiological examination of the condition of the shoulder joint engineering plants workers with the presence of the clinic osteoarthritis and periarthritis shoulder joint and assessment of mineral status of humerus at primary diagnosis and in the dynamics of observations on the basis of use of new technologies for digital osteodensitometry.The use of reference digital osteodensitometry made it possible to objectively assess the levels and topography of mineral distribution in the proximal humerus in the control group and in patients with osteopathy at various stages of clinical observation.The mineral density of the proximal humerus has differences depending on the sector and zone of study, being an objective criterion for the orientation of the restructuring of their structure and, consequently, the effectiveness of therapeutic measures.Digital reference osteodensitometry, as the most affordable method of early detection of osteoporosis or osteopennia, can be used in complex clinical and radiological examination of patients with suspected or already professionally caused osteopathy of the shoulder joint.

scholarly journals Long-Term Effects of Maternal Deprivation on the Neuronal Soma Area in the Rat Neocortex

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Milan Aksić ◽  
Nevena V. Radonjić ◽  
Dubravka Aleksić ◽  
Gordana Jevtić ◽  
Branka Marković ◽  
...  
Keyword(s):  
Animal Model ◽  
Morphological Changes ◽  
Maternal Deprivation ◽  
Retrosplenial Cortex ◽  
Control Group ◽  
Long Term Effects ◽  
Adult Rats ◽  
Adult Age ◽  
Neuronal Soma

Early separation of rat pups from their mothers (separatio a matrem) is considered and accepted as an animal model of perinatal stress. Adult rats, separated early postnatally from their mothers, are developing long-lasting changes in the brain and neuroendocrine system, corresponding to the findings observed in schizophrenia and affective disorders. With the aim to investigate the morphological changes in this animal model we exposed 9-day-old (P9) Wistar rats to a 24 h maternal deprivation (MD). At young adult age rats were sacrificed for morphometric analysis and their brains were compared with the control group bred under the same conditions, but without MD. Rats exposed to MD had a 28% smaller cell soma area in the prefrontal cortex (PFCX), 30% in retrosplenial cortex (RSCX), and 15% in motor cortex (MCX) compared to the controls. No difference was observed in the expression of glial fibrillary acidic protein in the neocortex of MD rats compared to the control group. The results of this study demonstrate that stress in early life has a long-term effect on neuronal soma size in cingulate and retrosplenial cortex and is potentially interesting as these structures play an important role in cognition.

scholarly journals Structural trace of adaptation in motive nuclei of spinal cord of rats in hypokinesia and after physical loading in the recovery period

2015 ◽  
Vol 6 (1) ◽  
pp. 9-16
Author(s):  
S. L. Popel ◽  
B. M. Mitckan ◽  
S. Z. Krasnopolsky ◽  
I. V. Melnik
Keyword(s):  
Spinal Cord ◽  
Functional State ◽  
Aerobic Capacity ◽  
Morphological Changes ◽  
Recovery Period ◽  
Morphometric Parameters ◽  
Control Group ◽  
Laboratory Rats ◽  
Physical Loading ◽  
Spinal Segments

The purpose of this paper is to study the morphological changes of neurocytes in spinal cord of rats in hypokinesia and subsequent physical loading. Studies were performed on 55 laboratory rats of Wistar line. Materials of the research were the anterior horns of the gray matter of L5-S2 spinal segments. Preparations stained by Nissl and Viktorov were examined histologically. Hypokinesia was modeled following on the author’s technique. It was established that during prolonged hypokinesia in neurocytes of spinal cord of rats morphological changes in cell size and shape of the motor nuclei of all segments under study have been recorded. The number of hypochromic, hyperchromic destructively unchanged and hyperchromic destructively altered neurocytes increase; shadow cells appears, as well as cases of satellitosis and neuronophagia. Decrease in of albumen synthetical neurocyte function has been recorded. Physical loading of the average aerobic capacity leads to normalization of structural and functional state of neurocytes and enhances the reparative processes, as evidenced by a number of positive changes in morphometric parameters: increase in the number of normochromic neurocytes and decreasing the number of hyper- and hypochromic neurocytes with destructive signs, absence of pyknotic forms. Morphological parameters of neurocytes and their nuclei after physical loading of average aerobic capacity do not differ from those in the control group of intact animals. In neurocytes of this group of rats RNA concentration increases by 12.6% compared to animals after prolonged hypokinesia. Neurocytes of spinal cord of rats after prolonged hypokinesia develop significant morphological changes which are characterized by emergence of a significant number of hyperchromic neurocytes with signs of destructive changes and shadow cells, as well as and hypochromic neurocytes with signs of destructive changes, reduction in size and change of shape of perikaryons of neurocytes and their nuclei. Morphological changes of neurocytes after prolonged hypokinesia are accompanied by violations of biosynthetic processes, as evidenced by RNA decrease in the cytoplasm of efferent neurocytes of spinal cord of rats. Physical loading of average aerobic capacity leads to normalization of structural and functional state of neurocytes and promotes the reparative processes suported by positive changes in morphometric parameters.

Exercise, diet, and the reinforcing value of food in an animal model of anorexia nervosa

2018 ◽  
Vol 72 (7) ◽  
pp. 1692-1703
Author(s):  
Ana de Paz ◽  
Pedro Vidal ◽  
Ricardo Pellón
Keyword(s):  
Body Weight ◽  
Progressive Ratio ◽  
Activity Wheel ◽  
Ratio Schedule ◽  
Control Group ◽  
Adult Rats ◽  
Laboratory Rats ◽  
Reinforcing Value ◽  
Male Wistar Rats ◽  
Breaking Points

Activity-based anorexia (ABA) develops when laboratory rats are subjected to a single meal per day and have access to an activity wheel for the remaining time. Here, we studied the contribution of exercise and diet to the reinforcing value of food during ABA development. Three groups of eight adult male Wistar rats were used: an ABA group with 21.5 hr (then 22 hr) of wheel access and 1 hr (then 30 min) of food access, a control group with the same time exposure to food but without exercise, and a yoked group to the ABA in terms of weight loss. Rats were daily tested on a progressive-ratio schedule to measure their motivation for food. ABA rats gradually reduced their body weight more than the food control group. Animals steadily increased their breaking points in parallel to losses in body weight, but no significant differences were found between groups. Adult rats can develop ABA, but their loss in weight neither resulted in a decrease of food intake nor in the motivation to obtain it.

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