scholarly journals Morphological changes in the thymus of rats of reproductive period on the background of the immunosuppressant cyclophosphamide administration

2016 ◽  
Vol 97 (4) ◽  
pp. 578-585
Author(s):  
I V Bobrysheva
Keyword(s):  
Morphological Changes ◽  
Fatty Degeneration ◽  
Reproductive Period ◽  
Relative Content ◽  
Image Analyzer ◽  
Relative Area ◽  
Thymic Involution ◽  
Laboratory Rats ◽  
Functional Zones ◽  
Functional Areas

Aim. To study the features of morphological changes of the thymus gland (thymus) of reproductive period rats amid the administration of the immunosuppressant cyclophosphamide.Methods. Thymus preparations were studied using an image analyzer based on CX-41 Olympus microscope. The morphological and functional areas of the thymus were determined relative areas (as a percentage) occupied by subcapsular, inner cortical zones of the cortex and thymic lobules medulla; cells distribution density, the percentage of cellular elements: lymphoblasts, small, medium and large lymphocytes, macrophages, mitotically dividing, destructively altered cells epithelioreticulocytes.Results. A statistically significant reduction in the relative area of subcapsular and inner cortical zones of the cortex, as well as an increase in this parameter in the thymic medullary parenchyma after 1-30 days after administration of cyclophosphamide were found. The cell density is reduced both in the cortex and in the medulla. Cytoarchitectonics of the thymus morphological and functional zones changes: a decrease in relative content of lymphocytes, mainly of young forms, as well as increase in the percentage of macrophages and destructively altered cells occurred. Maximum deviations of studied parameters was recorded after 1 and 7 days after drug administration. In 60 days after cytostatic administration morphometric characteristics of the thymus morphological and functional zones in laboratory rats do not have significant differences from the parameters in control animals of appropriate age.Conclusion. A single administration of cyclophosphamide leads to acute thymic involution, which is manifested in a decrease in the relative area of the cortex, lobules deformation, organ fatty degeneration, decrease in the relative content of lymphocytes, increase in the percentage of macrophages and destructively modified cells, which may be indicative of a high degree of reactivity of the animals thymus; results of the study may serve as morphologic evidence of the damaging effect of cyclophosphamide on the thymus lymphoid tissue.

scholarly journals Comparative characteristics of changes in subchondral bone of rats after antenatal glucocorticoid administration and modeling of osteoporosis

Morphologia ◽  
2021 ◽  
Vol 15 (3) ◽  
pp. 62-65
Author(s):  
O.A. Hryhorieva ◽  
P.V. Bohdanov ◽  
T.M. Matvieishyna ◽  
E.R. Skakovskiy
Keyword(s):  
Subchondral Bone ◽  
Bone Fractures ◽  
Morphological Changes ◽  
Control Group ◽  
Relative Area ◽  
Serial Sections ◽  
Mineral Density ◽  
Adult Rats ◽  
Laboratory Rats ◽  
Bone Trabeculae

Background. Osteoporosis is a progressive systemic bone disease that results in decreased bone mineral density and, as a result, increases the risk of bone fractures. Changes that occur in the subchondral bone in osteoporosis or because of hormones can cause degenerative changes in the articular cartilage that underlie osteoarthritis. Objective. The aim of the study was to identify and compare morphological changes that occur in the subchondral bone in experimental simulations of osteoporosis and in adult rats that were born from females that were exposed to glucocorticoid solution administration in the 3rd trimester of pregnancy. Methods. Tibias of 26 white mature laboratory rats were studied. In serial sections, the relative areas occupied by bone trabeculae and lacunae were calculated. Results. The relative area involving the bone trabeculae (23,2 ± 3,70%) statistically significantly decreased at 21 day in the group of animals that undergone a simulation of osteoporosis in comparison with the control group. Similar changes are observed in the group of experimental animals that were born from females that were exposed to glucocorticoid solution administration in the 3rd trimester of pregnancy. Conclusion. Thus, the results of the study demonstrate the similarity of morphological changes occurring in the subchondral bone in rats that undergone a simulation of osteoporosis and rats that were born from females that were exposed to glucocorticoid solution administration in the 3rd trimester of pregnancy.

REACTION OF CD68-POSITIVE RAT LIVER AND SPLEEN CELLS ON SILICON INTAKE WITH DRINKING WATER

2021 ◽  
pp. 34-43
Author(s):  
Evgeniia A. Grigoreva ◽  
Valentina S. Gordova ◽  
Valentina E. Sergeeva ◽  
Alina T. Smorodchenko
Keyword(s):  
Drinking Water ◽  
Cytoplasmic Membrane ◽  
Unit Area ◽  
Morphological Changes ◽  
Morphological Characteristics ◽  
Control Group ◽  
Silicon Compound ◽  
Average Cell ◽  
Laboratory Rats ◽  
Experimental Group

The article presents data on the long-term effect (nine months) of a silicon compound supplied with drinking water – nonahydrate sodium metasilicate (10 mg/l in terms of silicon), on CD68-positive macrophages in the liver and spleen of laboratory rats. Changes in the morphological characteristics of this cell population were found. There was a decrease in the average cell area (in the liver of the control group of rats, the average macrophage area was 179.23±5.94 microns2, and in the group receiving silicon with drinking water – 117.04±3.35 microns2; in the spleen-136.02±3.93 microns2 and 103.44±2.8 microns2, respectively). Macrophages in the liver preparations of the experimental group of rats had a fewer processes and a darker cytoplasmic membrane. The number of macrophages in the liver per unit area was comparable, for the control group of rats it was 18.78±1.24, and for the rats that received with water with the addition of silicon – 19.41±0.75 cells. CD68+ macrophages of the red splenic pulp in laboratory rats that received silicon also underwent the following morphological changes: they were located in a denser way and had fewer processes, while the number of macrophages per unit area was 73.7±2.3 for the control group, 91.6±5.0-for the experimental group, respectively. The distance between them did not change. There was a change in the intensity of CD68 expression on the surface of the cytoplasmic membrane and in the cytoplasm of liver and spleen macrophages. These changes can be interpreted as the adaptive ability of liver and spleen macrophages to silicon introduced with drinking water. Given the heterogeneity of the macrophage population in the liver and spleen, further studies using markers for different subpopulations of macrophages are needed to clarify their role in the response of tissues to silicon supplied with drinking water.

scholarly journals Geoecological Assessment of the State of Soils in Functional Zones of the Territory of the Eastern Administrative Okrug of Moscow, the Russian Federation

2021 ◽  
Vol 285 ◽  
pp. 06009
Author(s):  
S. V. Dubrova ◽  
I. I. Podlipskiy ◽  
P. S. Zelenkovskiy ◽  
P. I. Egorov ◽  
E. M. Nesterov
Keyword(s):  
Urban Development ◽  
The State ◽  
Point Of View ◽  
Functional Zone ◽  
Functional Zones ◽  
Preliminary Preparation ◽  
The Russian Federation ◽  
Functional Areas ◽  
Territorial Division ◽  
Functional Purpose

With the development of megalopolises, constant expansion of their borders and chaotic and – to a greater extent – unreasonable territorial division of lands, potential recreational areas are experiencing colossal anthropogenic load and can be found in the state of oppression, gradually moving into an industrial functional zone, from the environmental point of view. For the preservation of ecosystems and rational planning of urban development, it is necessary to pay special attention to the functional purpose and mode of use of the sites, which are the essence of zoning and governance in the field of urban development of land. This paper presents a geoecological assessment of the dynamics of changes in pollution halos among the functional areas of the Eastern Administrative Okrug of Moscow over the past 30 years. Geochemical series of pollutants were compiled with the help of methods of preliminary preparation and statistical data processing. A forecast of the spread of pollution in the surface horizon for the next 100 years is presented, taking into account the hydrogeological features of the territory of the Eastern Administrative Okrug of Moscow.

scholarly journals Features of copper accumulation in the soils of different functional areas of Novokuybyshevsk

2019 ◽  
Vol 8 (2) ◽  
pp. 67-71
Author(s):  
Alexander Igorevich Startsev
Keyword(s):  
Urban Areas ◽  
Copper Content ◽  
Residential Area ◽  
Soil Samples ◽  
Anthropogenic Sources ◽  
Copper Accumulation ◽  
Test Plot ◽  
Oil Refineries ◽  
Functional Zones ◽  
Functional Areas

The vegetation of the Samara Region is characterized by a relatively high content of copper. This biogeochemical feature is typical for natural and technogenic transformed ecosystems of the region. Copper is included in the group of elements of the 2nd hazard class. At certain concentrations it is necessary for the normal functioning of all organisms, but with increasing concentrations in the environment or in food it shows toxicity. The technogenic sources of copper include metalworking and machine-building enterprises, fertilizers, road and rail transport, wastewater, products of incomplete fuel combustion and refining characteristic of the Samara Region. The peculiarities of copper accumulation and distribution in soils of urban areas of the region are insufficiently studied. Previously, such studies were carried out only in certain areas of Samara. The paper analyzes in detail the level of technogenic copper pollution of the soil cover in Novokuybyshevsk. Ecological and geochemical studies in Novokuybyshevsk were carried out for 3 years (2016-2018) on 7 stationary test plots in its different functional areas and on 1 background test plot. The objects of research were soil samples taken from the upper humus horizon (layer 0-10 cm). The quantitative content of copper in soil samples was determined by the method of inversion voltammetry. A comparative analysis revealed territories of Novokuybyshevsk with relatively high, medium and low levels of copper in the soil. The first group included the Park Dubki in its elevated part, the old residential area on Kirov Street, the roadside area at the intersection of Pobeda Avenue and Dzerzhinsky Street (93,3-90,7 mg/kg). The second group with an average copper content (71,0-73,3 mg/kg) included the square Elochki and a new residential area on Ostrovsky Street. The third group with a relatively low copper content (38,3-54,7 mg/kg) included three sample areas: the lowland part of the Park Dubki, the industrial zone and the background area. The concentration of copper in the soils of all studied functional zones in Novokuybyshevsk has not reached the level of approximate permissible concentration (APC), but more than the clark of the world soils and the background indicators. The downward trend in the concentration of copper in the soils of most of the studied functional zones of Novokuybyshevsk in 2018 compared with 2016 and 2017 at the relatively low level of copper content in soils of the industrial area of the city allows to exclude oil refineries from the list of its major anthropogenic sources.

scholarly journals ОЦЕНКА УРОВНЯ ОЗЕЛЕНЕНИЯ ГОРОДА БИРОБИДЖАНА С ПРИМЕНЕНИЕМ МУЛЬТИСПЕКТРАЛЬНЫХ ДАННЫХ

2021 ◽  
Vol 13 (4) ◽  
Author(s):  
Д.М. Фетисов ◽  
Д.В. Жучков ◽  
М.В. Горюхин
Keyword(s):  
Green Infrastructure ◽  
Vegetation Index ◽  
Normalized Difference Vegetation Index ◽  
Multispectral Imaging ◽  
Natural Vegetation ◽  
Medium Size ◽  
Functional Zones ◽  
Functional Areas ◽  
The City ◽  
And Storage

The urban greenness distribution between functional areas of a medium-size city Birobidzhan was assessed. To this end, normalized difference vegetation index (NDVI) values were calculated based on Sentinel 2 multispectral imaging. Birobidzhan is characterized by a large scatter of NDVI values (from –0.5 to +1). Areas with high levels of greenery are prevalent. They are found in different types of functional zones, but are specific mainly to natural recreational, agricultural, and individual build-up zones as well as to special areas. The spatial distribution of green infrastructure is highly contrast. The downtown part as well as the industrial and storage zones feature a combination of built-up areas with dense woody vegetation, which is often represented by fragments of preserved natural vegetation. In addition, a feature of the contrast is that low level of tree greenness is characteristic for the built-up districts of the city. Thus, in the city of Birobidzhan, ecological functions are largely performed by the natural vegetation present in the natural recreational zones on 70% of the city's area.

scholarly journals THE COMPARATIVE MORPHOLOGICAL ANALYSIS OF THE NEPHROTOXICITY OF DOXORUBICIN AND NANOSTRUCTURED DOXORUBICIN IN CONDITIONS OF TRANSPLANTABLE CARCINOGENESIS

2018 ◽  
Vol 26 (2) ◽  
pp. 45-48
Author(s):  
I. N. Chairkin ◽  
N. V. Chairkina ◽  
O. N. Deryabina ◽  
V. Y. Medvezhonkov ◽  
O. V. Kalmin
Keyword(s):  
Morphological Analysis ◽  
Structural Changes ◽  
Morphological Changes ◽  
Control Group ◽  
Structural Components ◽  
Laboratory Rats ◽  
Chemotherapy Drugs ◽  
Morphological Evaluation ◽  
Comparative Morphological Analysis ◽  
Convoluted Tubules

The study was carried out on 120 mongrel white laboratory rats, to whom were transplanted the ascitiс Zaidel's hepatoma and which were divided into three equal groups. Of these, the first group of animals was a control group without treatment. In the second group, to animals doxorubicin was injected, in the third group, nanostructured doxorubicin was injected to the animals for 21 days. The aim of the study was to assess the degree of morphological changes in the tubules and glomeruli of the kidneys under the influence of nanostructured doxorubicin and doxorubicin in the condition of transplantable carcinogenesis. Given the nephrotoxicity of the chemotherapy drugs studied, the dynamics of structural changes in the tubules and glomeruli of the kidneys was studied. Conducted a comparative morphological evaluation of the changes occurring in the kidneys. The results of a morphological study of the structural components of the kidneys prove that doxorubicin and nanostructured doxorubicin have different effects on both the tubular apparatus and the glomeruli of the kidneys in the condition of transient carcinogenesis. The use of doxorubicin is accompanied by a relatively pronounced nephrotoxic effect, which is indicated by dystrophic and necrobiotic changes in the epithelium of convoluted tubules and the glomerular apparatus of the kidneys. The use of nanostructured doxorubicin is limited to moderately expressed dystrophic changes in the epithelium of the tubular apparatus of the kidneys, the glomerular apparatus remains intact at the same time. Thus, the use of the preparation of nanostructured doxorubicin for the treatment of the ascitic hepatoma of Zaidel in experimental animals has a less pronounced toxic and damaging effect on the renal parenchyma.

scholarly journals National park «Zyuratkul» (Chelyabinsk region) functional zones correction along habitat borders of vertebrate rare species

2021 ◽  
Vol 25 (2) ◽  
pp. 41-50
Author(s):  
A.R. Sibirkina ◽  
◽  
L.V. Trofimova ◽  
N.N. Kuzmishchev ◽  
◽  
...  
Keyword(s):  
Endangered Species ◽  
National Park ◽  
Climatic Conditions ◽  
Rare And Endangered Species ◽  
Literary Sources ◽  
Functional Zones ◽  
Rare And Endangered ◽  
The Russian Federation ◽  
Chelyabinsk Region ◽  
Functional Areas

Information on the18 species of vertebrates included in the Red Book of the Russian Federation and the Red Book of the Chelyabinsk Region habitats in the territory of the Zyuratkul National Park is presented. The developed system of functional zones in the Zyuratkul National Park is presented in order to ensure the safety of animals living on its territory. The analysis of literary sources describing the Red Book species of vertebrates inhabiting this territory is presented, on the basis of which the necessary requirements for their habitat are characterized and the climatic conditions formed in the studied territory are described. The general geographical features of the area are revealed, the laws of natural and economic territorial complexes are established. The established boundaries of the habitats of vertebrates are highlighted and plotted on schematic maps. Proposals have been developed to adjust the boundaries of previously defined functional areas. Proposals have been made for monitoring the number of rare and endangered species of vertebrates and monitoring anthropogenic load.

scholarly journals Rhein ElicitsIn VitroCytotoxicity in Primary Human Liver HL-7702 Cells by Inducing Apoptosis through Mitochondria-Mediated Pathway

2015 ◽  
Vol 2015 ◽  
pp. 1-19 ◽  
Author(s):  
Guy-Armel Bounda ◽  
Wang Zhou ◽  
Dan-dan Wang ◽  
Feng Yu
Keyword(s):  
Cytochrome C ◽  
Molecular Mechanisms ◽  
Caspase 3 ◽  
Morphological Changes ◽  
Fatty Degeneration ◽  
Apoptotic Cells ◽  
Dependent Manner ◽  
Caspase 9 ◽  
Cytochrome C Release ◽  
Induced Apoptosis

Objective. To study rhein-induced apoptosis signaling pathway and to investigate its molecular mechanisms in primary human hepatic cells.Results. Cell viability of HL-7702 cells treated with rhein showed significant decrease in dose-dependent manner. Following rhein treatment (25 μM, 50 μM, and 100 μM) for 12 h, the detection of apoptotic cells was significantly analyzed by flow cytometry and nuclear morphological changes by Hoechst 33258, respectively. Fatty degeneration studies showed upregulation level of the relevant hepatic markers (P< 0.01). Caspase activities expressed significant upregulation of caspase-3, caspase-9, and caspase-8. Moreover, apoptotic cells by rhein were significantly inhibited by Z-LEHD-FMK and Z-DEVD-FMK, caspase-9 inhibitor, and caspase-3 inhibitor, respectively. Overproduction of reactive oxygen species, lipid peroxidation, and loss of mitochondrial membrane potential were detected by fluorometry. Additionally, NAC, a ROS scavenger, significantly attenuated rhein-induced oxidative damage in HL-7702 cells. Furthermore, real-time qPCR results showed significant upregulation of p53, PUMA, Apaf-1, and Casp-9 and Casp-3 mRNA, with no significant changes of Fas and Cytochrome-c. Immunoblotting revealed significant Cytochrome-c release from mitochondria into cytosol and no change in Fas expression.Conclusion. Taken together, these observations suggested that rhein could induce apoptosis in HL-7702 cells via mitochondria-mediated signal pathway with involvement of oxidative stress mechanism.

scholarly journals Alloxan-Induced Diabetes Causes Morphological and Ultrastructural Changes in Rat Liver that Resemble the Natural History of Chronic Fatty Liver Disease in Humans

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Amanda Natália Lucchesi ◽  
Lucas Langoni Cassettari ◽  
César Tadeu Spadella
Keyword(s):  
Body Weight ◽  
Rat Liver ◽  
Morphological Changes ◽  
Glycosylated Hemoglobin ◽  
Weight Ratio ◽  
Liver Weight ◽  
Fatty Degeneration ◽  
High Serum ◽  
Ultrastructural Changes ◽  
Long Term Effects

Purpose. This study evaluated the long-term effects of alloxan-induced diabetes in rat liver.Methods. Thirty nondiabetic control rats (NC) and 30 untreated diabetic (UD) rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. Clinical and laboratory parameters were assessed. Fresh liver weight and its relationship with body weight were obtained, and liver tissue was analyzed.Results. UD rats showed sustained hyperglycemia, high glycosylated hemoglobin, and low plasma insulin. High serum levels of AST and ALT were observed in UD rats after 2 weeks, but only ALT remained elevated throughout the experiment. Fresh liver weight was equal between NC and UD rats, but the fresh liver weight/body weight ratio was significantly higher in UD rats after 14 and 26 weeks. UD rats showed liver morphological changes characterized by hepatic sinusoidal enlargement and micro- and macrovesicular hepatocyte fatty degeneration with progressive liver structure loss, steatohepatitis, and periportal fibrosis. Ultrastructural changes of hepatocytes, such as a decrease in the number of intracytoplasmic organelles and degeneration of mitochondria, rough endoplasmic reticulum, and nuclei, were also observed.Conclusion. Alloxan-induced diabetes triggered liver morphological and ultrastructural changes that closely resembled human disease, ranging from steatosis to steatohepatitis and liver fibrosis.

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