scholarly journals ISOLASI DAN KARAKTERISASI EKSTRAK ETANOL DAUN LEILEM (Clerodendrum minahassae Teijsm dan Binn.) DENGAN METODE SPEKTROFOTOMETRI ISOLATION AND CHARACTERIZATION ETHANOL EXTRACT OF LEILEM LEAVES (Clerodendrum minahassae Teijsm and Binn) WITH METHODS SPECTROPHOTOMETRIC

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Yuri Pratiwi Utami ◽  
Imrawati Imrawati ◽  
Abd Rasyid
Keyword(s):  
Positive Result ◽  
Ethyl Acetate ◽  
Bacterial Cell ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Phytochemical Analysis ◽  
Ftir Analysis ◽  
Isolation And Characterization ◽  
Ft Ir

ISOLASI DAN KARAKTERISASI EKSTRAK ETANOL DAUN LEILEM (Clerodendrum minahassae Teijsm dan Binn.) DENGAN METODE SPEKTROFOTOMETRIISOLATION AND CHARACTERIZATION ETHANOL EXTRACT OF LEILEM LEAVES (Clerodendrum minahassae Teijsm and Binn) WITH METHODS SPECTROPHOTOMETRICYuri Pratiwi Utami1), Imrawati1), Abd.Rasyid.D1)1)Program studi sarjana farmasi, Sekolah Tinggi Ilmu Farmasi MakassarJl. Perintis Kemerdekaan KM.13,7 Daya telp/fax. 0411-583190 Makassar [email protected] leaf (Clerodendrum minahassae Teijsm .And Binn.) have been reported in traditional medicine for the treatment of various deseases such as stomatch ache and ascariasis. In Minahassa, leilem leaf generally consumed as vegetables. The present study revealed that leilem leaf can inhibit the growth of escherhia coli because of the presence of flavonoid that can demage bacterial cell membranes. This study at characterization of isolated compounds from ethanol extract of leilem leaves (Clerodendrum minahassae Teijsm and Binn). Extraction was done maceration using 70% ethanol with the percentage of rendement was 10,19%. The phytochemical analysis of ethanol extract of leilem leaves showed a positive result of alkoloid, flavonoid, streroid and tanin. The ethyl acetate extract was selected and 2 grams of it was taken to continued to second conventional column chromatography and 5 fractions were obtained. Fraction 2 was choosen and continued to preparative and result 2 isolates. Isolate 2 that showed one spot on tlc with rf 0,66. Was continued for spectrofotometri uv-vis and ftir analysis. The spectrophotometer uv-vis maximum wavalength at 269,0 nm and 333,0 nm. The ftir data indicated the presence of functional O-H, C-H, C=C and C-O which is suspected ad flavonoid.Keywords : Isolation, Characterization, leilem leaf (Clerodendrum minahassae Teijsm and Binn)ABSTRAKDaun leilem (Clerodendrum minahassae Teijsm dan Binn.) dilaporkan yaitu sebagai obat tradisional untuk menyembuhkan beberapa penyakit, seperti sakit perut dan Ascariasis. Daun leilem biasanya dikonsumsi sebagai sayuran oleh masyarakat di Minahasa. Penelitian ini bertujuan untuk mengetahui profil (karakter) senyawa yang diisolasi dari ekstrak etanol daun leilem (Clerodendrum minahassae Teijsm dan Binn.) ekstrak diperoleh dari proses maserasi menggunakan pelarut etanol 70% dengan persen rendamen 10,19%. Hasil uji kandungan kimia ekstrak etanol daun leilem positif mengandung alkaloid, flavonoid, steroid dan tanin. Ekstrak etil astetat dipilih dan diambil 2 gram dilanjutkan untuk dilakukan pemisahan menggunakan komatografi kolom konvensional dan menghasilkan 5 fraksi. Fraksi 3 dipilih dilanjutkan kromatografi kolom konvensional kedua dan didapatkan 5 fraksi. Fraksi 2 dipilih dan dilanjutkan ketahapan kromatgrafi lapis tipis preparatif dan menghasilkan 2 isolat. Isolat 2 yang menunjukkan 1 noda tunggal pada profil KLT dengan niliai Rf 0,66 dilanjutkan untuk dianalisis dengan spektofometri UV-Vis dan FT-IR. Hasil analisis spektrofotometri UV-Vis isolat 2 mempunyai serapan maksimal pada panjang gelombang 269,0 nm dan 333,0 nm-1. Data FT-IR menunjukkan adanya gugus fungsi O-H, C-H, C=C dan C-O yang diduga merupakan senyawa flavonoid. Kata kunci: Isolasi, karakteriksasi, Daun Leilem(Clerodendrum minahassae Teijsm and Binn). 

Antimicrobial potentials, phytochemical screening and determination of bioactive compounds from Three Indian folk medicinal plants

2020 ◽  
Vol 16 ◽  
Author(s):  
V. Vanitha ◽  
S. Vijayakumar ◽  
S. Rajalakshmi
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Antimicrobial Agents ◽  
Ethyl Acetate Extract ◽  
Diffusion Method ◽  
Dilution Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Ft Ir

Aim: To investigate the antimicrobial efficacy of various solvents leaf extracts of Aegle marmelos, Plumbago zeylanica and Rhinacanthus nasutus against human pathogenic organisms such as Acinetobacter baumannii, Salmonella enteriditis, Bacillus cereus, Streptococcus pneumonia, Mycobacterium tuberculosis and Aspergillus fumigatous by agar well diffusion method. Background: Also, this is the first time report on MIC and MBC/MFC activities and identify the phytochemicals and functional groups using HPLC, GC-MS and FT-IR analysis. Objective: Minimum inhibitory concentration (MIC), minimum bactericidal (MBC/MFC) values were also carried out by the micro dilution method. Methods: Ethyl acetate extract of P. zeylanica showed better antimicrobial activity against the tested pathogens, especially for S. pneumoniae (40 mm) followed by ethyl acetate extract of R. nasutus (36 mm) against S. pneumoniae. While least inhibition was observed for aqueous extract of P.zeylanica against S. enteritis (10 mm). Results: The MIC ranged from 3.75 µg/ml to 60µg/ml and MBC/MFC 7.5 µg/ml to 60 µg/ml. Phytochemical analysis exhibited the presence of alkaloids, steroids, saponins, flavonoids, tannins, terpenes, phenolics and cardiac glycoside in all samples of selected plant extracts. Tannins, phenolics and glycoside were found only in the ethyl acetate extract of P.zeylanica. HPLC and GC-MS analysis of ethyl acetate leaf extract of P. zeylanica exposed ten major peaks and eleven compounds respectively. Chemical nature of the leaf extract was analysed by FT-IR spectrum. Conclusion: Ethyl acetate leaf extract of P. zeylanica showed better antimicrobial activity than other extracts as well as standard drugs. Therefore, this plant could be studied in detail for the identification of active biomolecules for antimicrobial agents.

scholarly journals Protoflavanones from the Wood Stem of Salvertia convallariodora

2017 ◽  
Vol 12 (4) ◽  
pp. 1934578X1701200
Author(s):  
Mariana L. de Mesquita ◽  
José E. de Paula ◽  
Laila S. Espindola ◽  
Luiz A. L. Soares ◽  
Tania M. G. da Silva ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
2D Nmr ◽  
Phytochemical Analysis ◽  
Brazilian Cerrado ◽  
Stem Wood ◽  
Full Characterization ◽  
Glucosyl Ester ◽  
First Time

Phytochemical analysis of the ethyl acetate extract of stem wood of Salvertia convallariodora A. St.-Hil. (Vochysiaceae), a Brazilian Cerrado species, led to the isolation and full characterization of three new non-aromatic B-ring flavanones (1-3) as well as the terpene mixture of sericic acid (4), 24-hydroxytormentic acid (5), 24-hydroxytormentic acid glucosyl ester (6), and sericoside (7), all identified for the first time from S. convallariodora. The structures of the new flavanones (1-3) were established from IR, LC-PDA-qTOF-MS, and NMR spectral data, including 2D NMR experiments.

scholarly journals ISOLATION AND CHARACTERIZATION OF TERPENOID COMPOUNDS FROM LEAVES OF Macaranga beccariana Merr.

2018 ◽  
Vol 16 (1) ◽  
pp. 22 ◽  
Author(s):  
Doni Prio Atmoko ◽  
Eva Marliana ◽  
Erwin Erwin
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Fraction ◽  
Gradient Elution ◽  
Isolation And Characterization ◽  
Rf Values ◽  
Ft Ir ◽  
Elution Method ◽  
Layer Chromatography ◽  
Chloroform Methanol

Isolation and characterization of terpenoid compounds from ethyl acetate fraction of Macaranga beccariana Merr. have been done. The separation process at ethyl acetate fraction using gravity column chromatography with gradient elution method. The obtained isolates were tested for purity using thin layer chromatography with various eluents namely n-hexane, chloroform, methanol, acetone and ethyl acetate with Rf values 0; 0.75; 0.80; 0.87 and 0.97 respectively. The terpenoids which were successfully isolated were white crystals with a mass of 0.0119 grams. UV analysis produced 1 peak at l 207.03 nm. Analysis of compounds with FT-IR indicated the functional groups O-H, aliphatic C-H, aliphatic C=C, C-O and C=O. Based on the results of the UV and FT-IR spectrum, the isolate compounds were thought to contain terpenoids.

scholarly journals ISOLATION AND CHARACTERISATION OF BERGENIN FROM ETHYL ACETATE EXTRACT OF FLUEGGEA VIROSA LEAVES

2020 ◽  
Vol 45 (6) ◽  
Author(s):  
C. T. Agber ◽  
T. A. Tor-Anyii ◽  
J. O. Igoli ◽  
J. V. Anyam
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Amorphous Solid ◽  
Isocratic Elution ◽  
Tropical Plant ◽  
Isolation And Characterization ◽  
Accessible Method ◽  
Vacuum Liquid Chromatography ◽  
C Nmr

Bergenin is an important constituent of Flueggea virosa (Euphorbiaceae), a tropical plant with several traditional uses. While there are numerous reports on the isolation and characterization of bergenin, a rapid, high-throughput, readily accessible method for the isolation and characterization of the compound locally has not been reported. Isocratic elution of ethyl acetate extract via vacuum liquid chromatography (VLC) with methanol produced a white amorphous solid (100 mg), which was successfully isolated from 250 g of the plant. On the basis of spectral data (1H, C NMR, COSY, HSQC and HMBC) and comparison with literature reports, the structure of this solid was shown to be bergenin, a dihydroisocoumarin derivative of glucopyranosyl gallic acid.

scholarly journals Isolasi Dan Karakterisasi Senyawa Steroid Dari Ekstrak Biji Mahoni (Swietenia mahagoni Jacq.)

2020 ◽  
Vol 7 (2) ◽  
pp. 6-11
Author(s):  
Fadillah Maryam ◽  
Subehan Subehan ◽  
Lilis Musthainah
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Seed Extract ◽  
Test Results ◽  
A Value ◽  
Chemical Content ◽  
Maximal Absorption ◽  
Ft Ir ◽  
Swietenia Mahagoni

Mahogany seeds (Swietenia mahagoni Jacq.) Have many benefits as drugs such as febrifuge, diabetes (Diabetes Mellitus), high blood pressure, fat decay, colds, colitis, diarrhea, wounds, and ulcers. Swietenia mahagoni Jacq Contain steroid compounds. This study aims to determine the characterization of steroid compounds isolated from mahogany seed extract (Swietenia mahagoni Jacq.). Extracts were obtained from maceration processes using n-hexane, ethyl acetate, and ethanol 70% solvents with percent of rendament of 6.34%, 7.34%, and 8% respectively. The test results for the chemical content of ethyl acetate extract positively contained steroids. 5 grams of extract was carried out using conventional column chromatography to produce 6 fractions. Fraction 6 were selected and continued with preparative thin layer chromotography and produced 2 isolates. Isolates P2b which showed one tonggal stain on the TLC profile with a value of Rf 0.50 were continued to be analyzed by UV-Vis and FT-IR spectrophotometry.The results of UV-Vis spectrophotometric isolates P2b had maximal absorption at a wavelength of 253.50 nm. FT-IR data shows the presence of functional groups C-H, C-O, C = O, C = C, and O-H whichare thought to be steroid compounds.

A Flavonoid compound of Turbinaria decurrens Bory with The Potential Antioxidant and Anticancer Activity

2021 ◽  
pp. 6207-6210
Author(s):  
Fitriyanti Jumaetri Sami ◽  
Nunuk Hariani Soekamto ◽  
Tatsufumi Okino ◽  
Firdaus Firdaus ◽  
Jalifah latip
Keyword(s):  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Ethyl Acetate Extract ◽  
Flavonoid Compound ◽  
Mtt Method ◽  
South Sulawesi ◽  
Isolation And Characterization ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ft Ir

Isolation and characterization of quercetin flavonoid compound from Dutungan Island, South Sulawesi Province, Indonesia has been successfully done from ethyl acetate extract. Extraction method used maceration, isolation used chromatography, anticancer activity with MTT method and antioxidant test used DPPH radical. Structure was discussed with the FT-IR, NMR spectrophotometer and compared with the literature. Total flavonoids from ethyl acetate extract were 4.8 mgEQ/g, IC50 value of antioxidant activity was 4.23 μg/ml using the DPPH method (2,2-diphenyl-1-picrylhydrazyl), and anticancer activity of H460 cells IC50 value was 10.95 μg/ml. The quercetin compound is potential as an anticancer and was first report in the T. decurrens Bory species.

scholarly journals Aktivitas Larvasida Ekstrak Daun Tumih (Combretocarpus rotundatus (Miq.) Danser) terhadap Larva Aedes aegypti

2019 ◽  
Vol 13 (1) ◽  
pp. 77
Author(s):  
Renhart Jemi ◽  
Royda Dara Ertini Damanik ◽  
Lies Indrayanti
Keyword(s):  
Aedes Aegypti ◽  
Ethyl Acetate ◽  
Functional Groups ◽  
Larvicidal Activity ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Leaf Extracts ◽  
Ft Ir

Penelitian ini bertujuan untuk mengukur kadar ekstrak daun tumih dan menguji aktivitas larvasidanya terhadap Aedes aegypti. Daun tumih dimaserasi dan difraksinasi dengan pelarut metanol, n-heksana, etil asetat, dan etanol. Aktivitas larvasida ekstrak diuji dengan konsentrasi 0, 5, 10, 25, 50, 75, dan 100 ppm. Hasil penelitian menunjukkan rendemen dari ekstraksi daun tumih pada berbagai larutan adalah sebagai berikut ekstrak metanol sebesar 15%, n-heksana 51%, etil asetat 35% dan etanol 85%. Hasil uji fitokimia menunjukkan bahwa ekstrak metanol daun tumih positif mengandung senyawa alkaloid, flavonoid, saponin, dan tanin. Pengujian larvasida ekstrak daun tumih menunjukkan pengaruh terhadap mortalitas larva Aedes aegypti. Aktivitas larvasida ekstrak daun tumih optimum pada ekstrak etil asetat dengan LC(50) = 24,54 ppm, ekstrak metanol LC(50) = 45,65 ppm, ekstrak etanol LC(50) = 46,77 ppm dan ekstrak n-heksana LC(50) = 48,97 ppm. Ekstrak etil asetat daun tumih merupakan ekstrak teraktif dalam aktivitas larvasidanya. Selanjutnya, analisis FT-IR menunjukkan adanya gugus fungsi C-H alkana dan C = C aromatik. Gugus fungsi tersebut diduga penyusun senyawa alkaloid, flavanoid, saponin, dan tanin. Hasil analisis LCMS mengindikasikan adanya 7 senyawa bioaktif yaitu hexadecyl-ferulate, 21-o-methyl toosendanopentaol, 23-acetate alismaketone, dehydroxy-24-acetate alisol, physanol, prosapogenin 2, dan stigmastan-3,6-dione Larvacide Activity of Tumih (Combretocarpus rotundatus (Miq.)Danser) Leaf Extracts against Aedes aegyptiAbstractThis research aimed to measure the content of Combretocarpus Rotundatus (Miq.) Danser leaf extracts and to test its larvicidal activity against Aedes aegypti. The leaves were macerated and fractionated using methanol, n-hexane, ethyl acetate, and ethanol. The extract contents from the leaves extraction were metanol extract of 15%, n-hexane extract of 51%, ethyl acetate extract of 35% and ethanol extract of 85%. The larvicidal activity of extracts was tested with concentration of 0, 5, 10, 25, 50, 75, and 100 ppm. Phytochemicals test exhibited that the methanol extract of Combretocarpus rotundatus (Miq.) Danser leaves contained alkaloids, flavonoids, saponins, and tannins. Larvicidal test conducted on the extracts exhibited an effect on the mortality levels against Aedes aegypti larvae. The larvicidal activity of leaf extracts was optimum in the ethyl acetate extract at LC(50) = 24.54 ppm, methanol extract at LC(50) = 45.65 ppm, ethanol extract at LC(50) = 46.77 ppm, and n-hexane extract at LC(50 = 48.97 ppm. It was found that the ethyl acetate extract was the most active larvicide. FT-IR analysis showed existing functional groups of C-H alkanes and C=C aromatics. Those functional groups were assumed to be flavanoid, alkaloid, saponin, and tannin constituents. Results of LC-MS analysis indicated 7 bioactive compounds i.e.hexadecyl-ferulate, 21-o-methyl toosendanopentaol, 23-acetate alismaketone, dehydroxy-24-acetate alisol, prosapogenin 2, and stigmastan-3,6- dione.

scholarly journals CHEMICAL CONSTITUENTS FROM ETHYL ACETATE EXTRACT OF SCOPARIA DULCIS LINN.

2018 ◽  
Vol 54 (2C) ◽  
pp. 278
Author(s):  
Ton Nu Lien Huong
Keyword(s):  
Ethyl Acetate ◽  
Spectral Data ◽  
Ethyl Acetate Extract ◽  
Chemical Constituents ◽  
Natural Compounds ◽  
Oroxylin A ◽  
Scoparia Dulcis ◽  
Isolation And Characterization ◽  
Nitrogen Containing Compounds

This research focuses on the isolation and characterization of eight natural compounds fromthe ethyl acetate extract of Scoparia dulcis L. collected in An Giang province, Vietnam. Sixflavonoid compounds: apigenin (1), luteolin (2), baicalein (3), oroxylin A (4), oroxylin A 7-O-β-D-glucoside (5) and vitexin (6) along with two nitrogen-containing compounds: 2,4-dihydroxy-1,4-benzoxazin-3-one (7) and uracil (8) were successfully isolated via the methods of naturalproduct extraction. The structures of these compounds were elucidated base on 1D-NMR and2D-NMR spectral data as well as MS and compared with those in the authentic reports. Thisstudy contributed new results to the phytochemical examination of Scoparia dulcis Linn.growing in Vietnam with the presence of the first isolated compounds as (3), (4), (5), (7) and (8).

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