scholarly journals Comparison of hot water and methanol extraction combined with ultrasonic pretreatment on antioxidant properties of two pigmented rice cultivars

Food Research ◽  
2019 ◽  
Vol 4 (2) ◽  
pp. 547-556
Author(s):  
U. Tobgay ◽  
P. Boonyanuphong ◽  
P. Meunprasertdee
Keyword(s):  
Conventional Method ◽  
Antioxidant Activities ◽  
Hot Water ◽  
Red Rice ◽  
Rice Varieties ◽  
Ultrasonic Pretreatment ◽  
Phenolic Contents ◽  
Pigmented Rice ◽  
Methanol Extraction ◽  
Glutinous Rice

Pigmented rice is considered as the good source of phytochemicals which contains more phenolic contents and higher antioxidant activities compared with non-pigmented rice. However, those phytochemicals are normally extracted using inorganic solvent, using water was rarely found. This study was aimed to compare extraction methods on antioxidant contents and activities of two types of Thai pigmented rice (black glutinous rice and red non-glutinous rice). Pigmented rice was extracted either conventional method alone or a combination of ultrasonic plus conventional method with different solvents (methanol and hot water). Phenolic contents were analyzed by spectrophotometric assay and high-performance liquid chromatography (HPLC). Antioxidant activities were investigated by radical scavenging capacity (ABTS and DPPH). The results showed that ultrasonic pretreatment significantly enhanced the total phenolic, flavonoid, anthocyanin contents and higher antioxidant activities compared with conventional extraction irrespective of solvents and rice varieties. Ultrasonic methanol (UM) extraction proved to extract significant higher content of all the compounds analyzed in both the rice varieties. In contrast, hot water (HW) extracts exhibited the lowest amount of bioactive compounds. However, there was no significant difference between methanol extraction alone and ultrasonic pretreatment with hot water extract on antioxidant contents for black glutinous rice. The contents of individual anthocyanins and flavonoids (cyanidin 3-O-glucoside, peonidin 3-O-glucoside, and quercetin) were significantly increased with ultrasonic pretreatment compare to the conventional method in black glutinous rice while failed to detect the red rice variety. The black glutinous rice contained significantly higher contents of all analyzed compounds and antioxidant activities than red rice. In conclusions, our results demonstrated that ultrasonic pretreatment significantly enhanced antioxidant extraction with higher activities compared with conventional extraction irrespective of solvents and rice varieties. Furthermore, the efficiency of ultrasonic together with hot water extraction was almost equal to methanol extraction which was the one effective solvent for extracting antioxidant.

scholarly journals Nutritional compositions, polyphenolic profiles and antioxidant properties of pigmented rice varieties and adlay seeds enhanced by soaking and germination conditions

2018 ◽  
Vol 8 (12) ◽  
pp. 561 ◽  
Author(s):  
Iyiola Oluwakemi Owolabi ◽  
Bandhita Saibandith ◽  
Santad Wichienchot ◽  
Chutha Takahashi Yupanqui
Keyword(s):  
Functional Properties ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Red Rice ◽  
Rice Varieties ◽  
Pigmented Rice ◽  
Germination Process ◽  
Rice Samples ◽  
New Compounds ◽  
Innovative Techniques

Background: Pigmented rice varieties are gaining interest due to their superior nutritional and phenolic properties compared to white rice varieties. With the rising risk of diabetes, cereals with higher protein contents should be consumed with rice. Adlay is one of the underutilized crops with higher protein contents and other functional properties. The germination process is one of the easy and innovative techniques for improving functional properties in seeds. In this study, two pigmented rice varieties and adlay seeds were soaked (24 h) and germinated for 12, 24, 36, and 48 h. The physicochemical properties, polyphenolic profiles, and the antioxidant activities of these samples were evaluated.Results: Purple rice (PR) demonstrated the highest values for polyphenolic contents, with 9 compounds detected for antioxidant activities. There were 6 compounds detected for red rice (RR). The adlay seeds had the least concentrations of phenolic compounds, with 6 compounds  and higher nutritional properties identified. New compounds were synthesized. Among the rice samples, 24 h soaking (S24) gave the best results for phenolic and antioxidant properties, with 24 h germination in adlay seeds. 48 h germination yielded better results for the nutritional values in all the samples.Conclusion: The present study demonstrates how the process of soaking is a cheap and less time-consuming process of improving bioactive compounds and antioxidant activities in pigmented rice varieties and adlay seeds. Keywords: polyphenols; antioxidant; pigmented rice; adlay; germination.

Phenolic Acids and Antioxidant Activities in Husk of Different Thai Rice Varieties

2010 ◽  
Vol 16 (4) ◽  
pp. 329-336 ◽  
Author(s):  
S. Butsat ◽  
S. Siriamornpun
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Rice Husk ◽  
Antioxidant Activities ◽  
Free Fraction ◽  
Total Phenolic ◽  
Rice Husks ◽  
Rice Varieties ◽  
Phenolic Contents ◽  
Pigmented Rice

This study was designed to investigate the free and bound phenolic acids as well as their antioxidant activities in husk of 12 Thai rice varieties consisting of pigmented rice and normal rice. The pigmented rice husk gave higher free total phenolic contents than normal rice husk. However, there was no significant difference in bound total phenolic contents between pigmented rice and normal rice husks. Ferulic and p-coumaric acids were the major phenolic acids in the free fraction of pigmented rice husks, whereas vanillic acid was the dominant phenolic acid in the free fraction of normal rice husks. On the other hand, p-coumaric acid was highly found in bound form of both pigmented and normal rice husks. The antioxidant activity of husk extracts was positively correlated with the total free phenolics content and individual of phenolic acids especially ferulic acid. On the basis of this study, it is suggested that the rice husk could be a potential phenolic acid source and may therefore offer an effective source of natural antioxidant. Our findings provide valuable information on phenolic acids composition and antioxidant activity of husk for further food application.

scholarly journals Anthocyanin, Lutein, Polyphenol Contents and Antioxidant Activity of Black, Red and White Pigmented Rice Varieties

2017 ◽  
Vol 1 (1) ◽  
pp. 43 ◽  
Author(s):  
Saravanan Ponnappan ◽  
Arun Thangavel ◽  
Omprakash Sahu
Keyword(s):  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Anthocyanin Content ◽  
Black Rice ◽  
Rice Varieties ◽  
Pigmented Rice ◽  
Polyphenol Compound ◽  
Glutinous Rice ◽  
Scavenging Capacity ◽  
Radical Scavenging Ability

<p><em>Colour rice varieties are rich in antioxidants and functional based properties such as anthocyanin, lutein and phenolic compounds. In this experiment, two of red pigmented (TPS-1and TKM-9), one white (glutinous rice) and black pigmented rice varieties are cultivated from India were analysed to determine their antioxidants and nutrition based functional properties. Based on the result, the anthocyanin content was very high on black rice than other variety contents up to 244.45</em><em> </em><em>mg/100 g. Polyphenol compound were varied significantly within the compared varieties. Highest polyphenol compound content (463.05 mg/100</em><em> </em><em>g) was found in the black rice and also showed rich antioxidant properties. Obviously, black rice rich source of lutein compound was also higher than other varieties where under the experimental condition. DPPH (Determination of 2, 20-diphenyl-1picrylhydrazyl radical scavenging ability) scavenging capacity starting from 69.46% to 76.4% ranged to level of remain DPPH. </em></p>

scholarly journals Protective effect of two Thai pigmented rice cultivars against H2O2-induced oxidative damage in HT-29 cell culture

Food Research ◽  
2022 ◽  
Vol 6 (1) ◽  
pp. 27-33
Author(s):  
P. Boonyanuphong ◽  
U. Tobgay
Keyword(s):  
Protective Effect ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Bioactive Compound ◽  
Red Rice ◽  
Black Rice ◽  
Pigmented Rice ◽  
Toxic Concentration ◽  
Dichlorofluorescin Diacetate ◽  
Ht 29

Radicals derived from exogenous and endogenous sources are considered to be the principal cause of genetic damage. Exogenous and endogenous radicals participate in the reactive oxygen species (ROS) formation, which leads to damages in the DNA, RNA, proteins and lipids. However, dietary compounds, mainly from pigmented rice, are an essential source of antioxidants that help protect cells from damage. This study seeks to determine the antioxidant properties and cytoprotective effect of two Thai pigmented rice extracts namely the glutinous black rice (native name: Neaw dum moa37) and red rice (native name: Hom gradung-nga57) on H2O2-induced damage in HT-29 cells. The bioactive compound contents, as well as antioxidant activities of both rice extracts, were investigated. The protective effect of rice extracts on H2O2-induced damage was executed following the co-incubation method. HT-29 cells were exposed to H2O2 and different rice extract concentrations for 3 h and an MTT assay was used to measure the viability of the cell. The ROS level was determined using the 2′,7′-dichlorofluorescin diacetate (DCFDA). The result showed that glutinous black rice extract contained significantly higher contents of all analysed antioxidants and activities than red rice extract. Glutinous black rice showed a higher cytotoxic effect compared to red rice. At the non-toxic concentration of both rice extracts, the HT-29 cells were guarded against the H2O2 induced oxidative stress. Besides, the intracellular ROS accumulation result from H2O2 exposure was significantly reduced in the presence of rice extracts for both glutinous black rice and red rice compared to control. Hence, this study has demonstrated the potential properties of both pigmented rice extracts in alleviating H2O2-mediated damage in HT-29 cells.

scholarly journals Discovery of Functional SNPs via Genome-Wide Exploration of Malaysian Pigmented Rice Varieties

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Rabiatul-Adawiah Zainal-Abidin ◽  
Norliza Abu-Bakar ◽  
Yun-Shin Sew ◽  
Sanimah Simoh ◽  
Zeti-Azura Mohamed-Hussein
Keyword(s):  
Breeding Program ◽  
Snp Analysis ◽  
Rice Seed ◽  
Rice Breeding ◽  
Red Rice ◽  
Rice Varieties ◽  
Protein Coding ◽  
Pigmented Rice ◽  
Genome Wide ◽  
Variation Database

Recently, rice breeding program has shown increased interests on the pigmented rice varieties due to their benefits to human health. However, the genetic variation of pigmented rice varieties is still scarce and remains unexplored. Hence, we performed genome-wide SNP analysis from the genome resequencing of four Malaysian pigmented rice varieties, representing two black and two red rice varieties. The genome of four pigmented varieties was mapped against Nipponbare reference genome sequences, and 1.9 million SNPs were discovered. Of these, 622 SNPs with polymorphic sites were identified in 258 protein-coding genes related to metabolism, stress response, and transporter. Comparative analysis of 622 SNPs with polymorphic sites against six rice SNP datasets from the Ensembl Plants variation database was performed, and 70 SNPs were identified as novel SNPs. Analysis of SNPs in the flavonoid biosynthetic genes revealed 40 nonsynonymous SNPs, which has potential as molecular markers for rice seed colour identification. The highlighted SNPs in this study show effort in producing valuable genomic resources for application in the rice breeding program, towards the genetic improvement of new and improved pigmented rice varieties.

Phenolic compounds and antioxidant activities in dehusked and polished pigmented rice varieties

2019 ◽  
Vol 56 (3) ◽  
pp. 263-284
Author(s):  
Rajarajeswari Jayaraman ◽  
Chandrasekhar Yadavalli ◽  
Vasudeva Singh ◽  
Farhath Khanum
Keyword(s):  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Carotenoid Content ◽  
Moderate Increase ◽  
Red Rice ◽  
Rice Varieties ◽  
Polished Rice ◽  
Total Carotenoid Content ◽  
Anti Oxidant

Present study was conducted to characterise eight red rice varieties in their de-husked and polished form for their phytochemical contents and anti-oxidant activities. Ferulic acid, gallic acid, quercetin, vanillic acid and rutin were identified in their free phenolic extracts. Dehusked rice free phenolics prevented free radical formation, metal chelation ferric to ferrous reduction and also influenced the reducing power. In polished rice, a moderate increase in antioxidant properties was noticed. The methanol extract showed higher phenolic content and anti-oxidant activities compared to extracts made in other solvents. Dehusked form of Kamdhari, Black basumati, Sirsi and Jyothi varieties exhibited higher amount of phytochemical components and antioxidant activities among the varieties. In dehusked form, total carotenoid content was more in Aravadan pillai and GK-4 varieties, whereas ascorbic acid content was high in Karisale, Kamdhari, Black basmati, Sirsi and Kasubai varieties. Thus, the dehusked and polished rice of these varieties with sufficient phenolic contents appear to be more useful as compared to normal rice with respect to their antioxidant properties.

scholarly journals Java Red Rice (Oryza sativa L.) Nutritional Value and Anthocyanin Profiles and Its Potential Role as Antioxidant and Anti-Diabetic

2021 ◽  
Vol 21 (4) ◽  
pp. 968
Author(s):  
Ayu Tri Agustin ◽  
Anna Safitri ◽  
Fatchiyah Fatchiyah
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Nutritional Value ◽  
Red Rice ◽  
Frap Assay ◽  
Rice Varieties ◽  
Total Anthocyanin ◽  
Antioxidant Power ◽  
Pigmented Rice ◽  
Ic50 Value

This study investigates nutritional value, amino acid profile, and total anthocyanin in pigmented rice as an antioxidant and anti-diabetic agent. Six rice varieties were extracted using 0.1% HCl in methanol, namely four red rice, one black rice, and one white rice. Rice extract was used for proximate analysis and amino acid profiling. Total anthocyanin was measured and identified by ultraviolet-visible (UV-Vis) spectrophotometer and thin-layer chromatography (TLC). The antioxidant activity was determined using Ferric-reducing antioxidant power (FRAP) assay, and the α-amylase enzyme-inhibited by anthocyanin extract of red rice as anti-diabetic was measured. The study result showed that the proximate level (carbohydrate, protein, lipid, water, and ash) in pigmented rice was different. Cempo merah red rice is a source of amino acids, both essential and non-essential amino acids that act as good nutrition. The highest total anthocyanin level between red rice varieties of 10.87 mg/g was found in Aek sibundong red rice. High biological function activities as an antioxidant were indicated by Aek sibundong red rice with an IC50 value of 6.65 µg/mL. Aek sibundong red rice shows the lowest IC50 value of 144.46 µg/mL in anti-diabetic activity. Thus, Aek sibundong red rice may have the potential as α-amylase inhibitor for diabetes prevention.

Evaluation of antioxidant activities and phenolic subtype contents of ethanolic bran extracts of Thai pigmented rice varieties through chemical and cellular assays

2014 ◽  
Vol 50 (4) ◽  
pp. 990-998 ◽  
Author(s):  
Worawat Surarit ◽  
Chalerm Jansom ◽  
Nusiri Lerdvuthisopon ◽  
Supranee Kongkham ◽  
Pintusorn Hansakul
Keyword(s):  
Antioxidant Activities ◽  
Rice Varieties ◽  
Pigmented Rice ◽  
Cellular Assays

scholarly journals Anthocyanin Content Characterization on Pigmented Local Rice as Genetic Resources of Functional Food

2018 ◽  
Vol 24 (2) ◽  
pp. 125
Author(s):  
Kristina Dwiatmini ◽  
Higa Afza
Keyword(s):  
Genetic Resources ◽  
Functional Food ◽  
High Performance ◽  
Anthocyanin Content ◽  
Red Rice ◽  
Black Rice ◽  
Rice Varieties ◽  
Local Varieties ◽  
White Rice ◽  
Pigmented Rice

<p>Local varieties of pigmented rice are highly valuable genetic resources as a functional food in the future. Pigmented rice contains anthocyanins which function as nutrient bioactive components, called antioxidants. The aim of this research was to characterize anthocyanin content on 27 accessions of pigmented local rice, 2 varieties of released red rice varieties (Aek Sibundong and Inpari<br />24), and 1 control varieties of white rice, Ciherang. The content of anthocyanin was analyzed by using High Performance Liquid Chromatography (HPLC) with cyanidin-3-glucoside as a standard. The results showed that local rice Aen Metan and Melik had the highest anthocyanin content were reached 0.7953 mg/g and 0.7806 mg/g. These content were higher than 2 released red rice varieties, Aek Sibundong and Inpari 24 which had anthocyanin content reached 0.6496 mg/g and 0.4423 mg/g, respectively. Aen Metan and Melik were local black rice varieties and frequently have used as a parent in the breeding program. The white rice as control, Ciherang was showed the lowest anthocyanin content. Four pigmented local rice, Baliman Putih, Sari Kuning, Karamanting, and Iden had higher anthocyanin content than the two released red rice breeding varieties.</p>

scholarly journals Total Phenolic Contents and Antioxidant Activities of Different Herbal Tea Extracts from Turkey

2019 ◽  
Vol 3 (2) ◽  
pp. 66-72
Author(s):  
Keyword(s):  
Green Tea ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Hot Water ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Herbal Tea ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Dpph Radical Scavenging

: The aim of the present study is to determine the total phenolic contents (TPC), DPPH radical scavenging activities, and reducing power of commercially consumed herbal teas including sage tea (ST), blackberry tea (BT), apple tea (AT), Echinacea tea (ET), lime tea (LT), rosehip tea (RT), and green tea (GT) in Turkey. For this purpose, various solvents were used for extraction of the tea samples and the results were compared in terms of total phenolic contents and antioxidant activities, as well. Hot water is found as the best solvent for the extraction of total phenolics from ST, BT, AT, and RT, respectively. On the other hand, the highest TPC values are obtained with the extraction of ET, LT, and GT in the presence of aqueous acetone (50%) solvent system. Generally all samples gave high DPPH radical scavenging activities depending on their extraction solvents. The highest radical scavenging activity was found as 94.92 ± 0.01 % for ST in ethanol, while the lowest activity was obtained as 5.98 ± 0.14 % for BT with water extract. Ferric reducing antioxidant assay results are closed each other and the highest activity (0.27 ± 0.74 mM Trolox) was observed in the ethanol extract of ST. Antioxidant activities and TPC of the herbal tea samples varied depending on the extraction solvent. Water or aqueous organic solvent extracts of sage tea and green tea samples gave high TPC values and antioxidant activities.

Sign in / Sign up

Export Citation Format

Share Document