scholarly journals Extraction and optimization of chitosan from razor clam (Ensis arcuatus) shells by using response surface methodology (RSM)

Food Research ◽  
2019 ◽  
Vol 4 (3) ◽  
pp. 674-678 ◽  
Author(s):  
A.I. Zamri ◽  
N.F. Latiff ◽  
Q.H. Abdullah ◽  
F. Ahmad
Keyword(s):  
Molecular Weight ◽  
Response Surface Methodology ◽  
Optimum Condition ◽  
Response Surface ◽  
Ash Content ◽  
Chemical Extraction ◽  
Razor Clam ◽  
Degree Of Deacetylation ◽  
Randomized Design ◽  
Two Factors

Chitin can be found in animal source especially arthropods such as crustacean, mollusk and insect, as well as in plant source such as fungi. Chitosan is obtained from chitin after the acetyl group is removed from chitin structure through deacetylation step and has wide application in various fields (food, cosmetics and pharmaceutical). In this study, chitosan was extracted from razor clam shells, where the extraction conditions were optimized. Two- factors of randomized D-optimal design was used to determine the optimum condition for the extraction of chitosan from razor clam (Ensis arcuatus) by using response surface methodology (RSM). The chemical extraction was optimized using five levels with two factors which were the deacetylation time (2,4,6,8,10 hrs) and deacetylation temperature (50, 60, 70, 80 and 90ºC). A randomized design suggested by Design Expert software was implemented with four responses evaluated: yield (%); degree of deacetylation (%); molecular weight (kDA); and ash content (%). Time (h) and temperature (ºC) of the deacetylation significantly (p<0.05) affected the yield (%), degree of deacetylation (DDA) (%), molecular weight (Mw) (kDA) and ash content (%) of the chitosan extracted. The optimum conditions for the chitosan extraction were at the respective deacetylation time and temperature of 6h and 70ºC with actual values of yield (%), degree of deacetylation (%), molecular weight (kDA) and ash content (%) of 19.903±2.367, 50.113±0.902, 476.727±13.603, 8.517±2.094, respectively. The optimum condition for the chitosan extraction was experimentally verified and valid for further analysis.

Controlling Molecular Weight and Degree of Deacetylation of Chitosan by Response Surface Methodology

2002 ◽  
Vol 50 (7) ◽  
pp. 1876-1882 ◽  
Author(s):  
Kwon T. Hwang ◽  
Soon T. Jung ◽  
Gee D. Lee ◽  
Manjeet S. Chinnan ◽  
You S. Park ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Degree Of Deacetylation

scholarly journals Optimasi Kondisi Proses Pembuatan Manisan Kering Cabai Merah (Capsicum annuum L.) dengan Menggunakan Response Surface Methodology (RSM)

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Hisworo Ramdani ◽  
Noli Novidahlia ◽  
Ulif Yuhana
Keyword(s):  
Response Surface Methodology ◽  
Vitamin C ◽  
Optimum Condition ◽  
Optimal Condition ◽  
Response Surface ◽  
Capsicum Annuum ◽  
Sugar Solution ◽  
Two Factors ◽  
Sensory Color ◽  
Test Color

The aims of the research were to determine the optimum condition of  submerging time in 2% CaCl2 and various sugar solutions on sweetened dried red chili characteristics using Response Surface Methodology (RSM). Red chili were  submerged in 2% CaCl2 for 8, 10, 15, 20, and 22 minutes, and in 56%, 60% 70%, 80% and 84% sugar solutions.  The output combination of two factors are 13 run units.  The analysis included physics (color), chemical (moisture, vitamin C, total sucrose), hedonic test (color, taste, texture), descriptive test (color, sweetness, spiciness, texture).  The results showed that submerged time in 2% CaCl2 had significant effect on sweetness of sweetened dried red chili. The optimal condition processing of sweetened dried red chili was reached on combination of submerged time in 2% CaCl2 8 minutes and in 74% sugar solution with chroma 21.16, moisture 8.88 % db, vitamin C 9.34 mg/100 g, total sucrose  50°Brix,  hedonic score: color 5.03 (rather like), taste 4.43 (neutral), texture 4.42 (neutral), and descriptive sensory: color 5.24 (rather red), sweetness 4.05 (neutral), spiciness 5.05 (rather spicy), and texture 4.53 (rather rubbery).Keywords: optimization, sweetened, red chili, RSM

scholarly journals EKSTRAKSI GLUKOMANAN DARI UMBI PORANG (Amorphophallus paeniifolius (Dennst.) Nicolson)

KOVALEN ◽  
2017 ◽  
Vol 3 (3) ◽  
pp. 234
Author(s):  
Elis Setiawati ◽  
Syaiful Bahri ◽  
Abd. Rahman Razak
Keyword(s):  
Molecular Weight ◽  
Moisture Content ◽  
Isopropyl Alcohol ◽  
Ash Content ◽  
Test Characteristics ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Level 1

This study aims to determine the temperature and the ratio of tuber flour porang using isopropyl alcohol precipitator in producing glucomannan extract with the highest yield. The study was designed using completely randomized design (CRD), which consists of two factors: the temperature of the extraction consists of seven levels (45, 55, 65, 75, 85, 95, and 105 0C) and the ratio of tuber flour porang by precipitating isopropyl alcohol consists of 5 level (1: 7, 1:10, 1:13, 1:16, and 1:19 (w/v). each done in duplicate. parameters measured were yield characteristics glucomannan and the results showed that the extraction of the best glucomannan obtained at a temperature of 95 0C with a yield of 41.614%. the ratio of starch tuber porang best isopropyl alcohol is 1:19 (w/v) with a yield of 45.167% glucomannan. Test characteristics of glucomannan gained 6.6% moisture content, the ash content of 0.8%, and a molecular weight of 0.726 x 104 g/mol.Keywords: porang tuber flour, shaking temperature, glucomannan.

Optimization of Ultrasonic Wave Extraction Technology of Polysaccharides from Auricularia auricula by Response Surface Methodology and Molecular Weight Distributions

2011 ◽  
Vol 183-185 ◽  
pp. 1852-1858
Author(s):  
Xin Zhao ◽  
Fang Zhou ◽  
Lei Zhu ◽  
Zhen Yu Wang
Keyword(s):  
Molecular Weight ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Ultrasonic Wave ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Extraction Time ◽  
Extraction Technology ◽  
Auricularia Auricula ◽  
Two Factors

In order to optimize the ultrasonic wave extraction technique of polysaccharides from Auricularia auricula, the technique conditions were carried out by response surface methodology. Then the molecular weight distribution of AAP extracted were studied. Two factors, ultrasonic wave and extraction time in water were screened from six factors . The singal-order tests and integrated curvature detection of the two factors were carried out. After that, the experiment domain approached to the optimum point, the optimum technology conditions of ultrasonic wave extraction of AAP were determined by central composite design: ultrasonic wave power 908W, extraction time 100°C, and the AAP yield of ultrasonic wave extraction approached to 24%. It was indicated that ultrasonic wave can promote the yield of the large molecular weight components by the analysis of molecular weight distribution.

Optimization of Aqueous Two Phase Extraction of Proteins from Litopenaeus Vannamei Waste by Response Surface Methodology Coupled Multi-Objective Genetic Algorithm

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
P. Saravana Pandian ◽  
S. Sindhanai Selvan ◽  
A. Subathira ◽  
S. Saravanan
Keyword(s):  
Genetic Algorithm ◽  
Molecular Weight ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Litopenaeus Vannamei ◽  
Value Added ◽  
Phase System ◽  
Two Phase ◽  
Multi Objective ◽  
Multi Objective Genetic Algorithm

Abstract Waste generated from industrial processing of seafood is an enormous source of commercially valuable proteins. One among the underutilized seafood waste is shrimp waste, which primarily consists of head and carapace. Litopenaeus vannamei (L. vannamei) is the widely cultivated shrimp in Asia and contributes to 90 % of aggregate shrimp production in the world. This work was focused on extraction as well as purification of value-added proteins from L. vannamei waste in a single step aqueous two phase system (ATPS). Polyethylene glycol (PEG) and trisodium citrate system were chosen for the ATPS owing to their adequate partitioning and less toxic nature. Response surface methodology (RSM) was implemented for the optimization of independent process variables such as PEG molecular weight (2000 to 6000), pH (6 to 8) and temperature (25 to 45 °C). The results obtained from RSM were further validated using a Multi-objective genetic algorithm (MGA). At the optimized condition of PEG molecular weight 2000, pH 8 and temperature 35 °C, maximum partition coefficient and protein yield were found to be 2.79 and 92.37 %, respectively. Thus, L. vannamei waste was proved to be rich in proteins, which could be processed industrially through cost-effective non-polluting ATPS extraction, and RSM coupled MGA could be a potential tool for such process optimization.

scholarly journals Optimization of Eugenol Extraction from Clove Oil using Response Surface Methodology

2015 ◽  
Vol 9 (11) ◽  
pp. 68
Author(s):  
Widayat Widayat ◽  
Hadiyanto Hadiyanto ◽  
Bambang Cahyono ◽  
Ngadiwiyana Ngadiwiyana
Keyword(s):  
Response Surface Methodology ◽  
Optimum Condition ◽  
Central Composite Design ◽  
Response Surface ◽  
Design Method ◽  
Main Process ◽  
Clove Oil ◽  
Design Variables ◽  
Central Composite

The objective of this research was to obtain optimum condition of eugenol production from clove oil using a central composite design method. The main process occured in the eugenol production was saphonification and neutralization processes. In order to optimize these processes, the ratio of NaOH/clove oil and temperature were studied as design variables i.e. ratio of NaOH/clove oil=1:2.5-1:3.5 while temperature was varied between 40 and 60oC. The yield of eugenol was considered as the main response in of this experiment. The result showed that the optimum condition was achieved when the temperature and the ratio of NaOH/clove oil were 50oC and 2.75:1, respectively and the yield was 39.17%.<br />

Optimization of Ultrasound Extraction of Nitrite Nitrogen in Sediment by Response Surface Methodology

2014 ◽  
Vol 955-959 ◽  
pp. 1466-1470 ◽  
Author(s):  
Dan Liu ◽  
Fang Yu ◽  
Wei Tan ◽  
Gui Zhen Li ◽  
Min Yang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Optimum Condition ◽  
Response Surface ◽  
Process Condition ◽  
Extraction Time ◽  
Ultrasonic Power ◽  
Liquid Ratio ◽  
Ultrasound Extraction ◽  
Nitrite Nitrogen ◽  
Solid Liquid

Response surface methodology based on single factors was used to optimize the process condition for extraction of nitrite nitrogen in sediment . The results indicated that the extraction amount of nitrite nitrogen was 9.5μg /g under the optimum condition of extraction time of 44min, ultrasonic power of 280W, solid-liquid ratio of 1:19,which was closed to the predicated yield of 9.3μg/g. The process can be used for the extraction of nitrite nitrogen in sediments.

Extraction of Flavonoids from Clovers

2012 ◽  
Vol 195-196 ◽  
pp. 360-363
Author(s):  
Chun Gang Chen ◽  
Fen Xia Han ◽  
Yuan Zhang ◽  
Yu Zhong Shi
Keyword(s):  
Response Surface Methodology ◽  
Optimum Condition ◽  
Central Composite Design ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Response Surfaces ◽  
Second Order ◽  
Order Model ◽  
Solid Ratio ◽  
Central Composite

The extraction of flavonoids from clovers was optimized to maximize flavonoid yield Y in this study. A central composite design of response surface methodology involving extracting time, liquid-solid ratio, extracting temperature and ethanol concentration was used, and second-order model for Y was employed to generate the response surfaces. The optimum condition for Y was determined as follows: extracting time 24min, liquid-solid ratio 20, extracting temperature 80°C, and ethanol concentration 72%. Under the optimum condition, the flavonoid yield was 2.49%.

Sign in / Sign up

Export Citation Format

Share Document