Optimization of Ultrasonic Wave Extraction Technology of Polysaccharides from Auricularia auricula by Response Surface Methodology and Molecular Weight Distributions

2011 ◽  
Vol 183-185 ◽  
pp. 1852-1858
Author(s):  
Xin Zhao ◽  
Fang Zhou ◽  
Lei Zhu ◽  
Zhen Yu Wang
Keyword(s):  
Molecular Weight ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Ultrasonic Wave ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Extraction Time ◽  
Extraction Technology ◽  
Auricularia Auricula ◽  
Two Factors

In order to optimize the ultrasonic wave extraction technique of polysaccharides from Auricularia auricula, the technique conditions were carried out by response surface methodology. Then the molecular weight distribution of AAP extracted were studied. Two factors, ultrasonic wave and extraction time in water were screened from six factors . The singal-order tests and integrated curvature detection of the two factors were carried out. After that, the experiment domain approached to the optimum point, the optimum technology conditions of ultrasonic wave extraction of AAP were determined by central composite design: ultrasonic wave power 908W, extraction time 100°C, and the AAP yield of ultrasonic wave extraction approached to 24%. It was indicated that ultrasonic wave can promote the yield of the large molecular weight components by the analysis of molecular weight distribution.

Preparation of rapeseed protein hydrolysates with ACE inhibitory activity by optimization and molecular weight distribution of hydrolysates

2017 ◽  
Vol 42 (4) ◽  
Author(s):  
Asif Wali ◽  
Haile Ma ◽  
Muhammad Tayyab Rashid ◽  
Qui Fang Liang
Keyword(s):  
Molecular Weight ◽  
Response Surface ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Inhibitory Activity ◽  
Proteolytic Enzymes ◽  
Ace Inhibitory Activity ◽  
Rapeseed Protein Hydrolysates ◽  
Hydrolysis Conditions ◽  
Ace Inhibitory

AbstractObjective:The main purpose of this study was to screen effective proteolytic enzymes for producing hydrolysates from rapes protein, and to optimize hydrolysis conditions using response surface design to prepare hydrolysates with maximum ACE inhibitor activity.Methods:RSM design was successfully applied to the hydrolysis conditions on the basis of single factor experiments which further derived a statistical model for experimental validation. The molecular weight distribution of rapeseed protein hydrolysates with different degree of hydrolysis was also investigated.Results:All the proteolytic enzymes tested produced hydrolysates that possessed ACE inhibitory activity. Aiding RSM design the highest ACE inhibitory activity 56.3% was achieved under optimum hydrolysis conditions at the hydrolysis time, pH, hydrolysis temperature, and enzyme dosage were at 90.11 min, 8.88, 50°C and 3580.36 UgConclusion:Enzymatic hydrolysis and response surface methodology found good techniques in order to achieve hydrolysates with maximum ACE inhibitory activity. The findings of current research suggested that the hydrolysates obtained under optimized conditions could be utilized to formulate nutraceuticals and pharmaceuticals

scholarly journals Optimization of extraction technology of polysaccharide from foxtail millet using response surface methodology

2014 ◽  
Vol 20 (4) ◽  
pp. 579-585 ◽  
Author(s):  
Ai-Shi Zhu ◽  
Jin-Na Ye ◽  
Fei-Na Yan
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Foxtail Millet ◽  
Polynomial Equation ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Extraction Technology ◽  
Solid Ratio ◽  
Experimental Yield ◽  
Optimum Extraction

The experiment of extraction of polysaccharide from foxtail millet was investigated. Response Surface Methodology (RSM),based on a threelevel, three variablesBox-Behnken design (BBD), was employed to obtain the best possible combination of liquid-solid ratio(X1: 15.0-25.0 mL?g-1),extraction time (X2: 1.5-2.5h), and extraction temperature (X3: 65.0-75.0?C)for maximum polysaccharide yields. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis and also analyzed by appropriate statistical methods (ANOVA). The optimum extraction conditions were as follows: liquid-solid ratio 20.7mL?g-1, extraction time 2.0h, extraction temperature 72.3?C. Under these conditions, the experimental yield was 8.08mg?g-1, which is well in close agreement with8.02mg?g-1predicted value by the model.

scholarly journals Extraction and optimization of chitosan from razor clam (Ensis arcuatus) shells by using response surface methodology (RSM)

Food Research ◽  
2019 ◽  
Vol 4 (3) ◽  
pp. 674-678 ◽  
Author(s):  
A.I. Zamri ◽  
N.F. Latiff ◽  
Q.H. Abdullah ◽  
F. Ahmad
Keyword(s):  
Molecular Weight ◽  
Response Surface Methodology ◽  
Optimum Condition ◽  
Response Surface ◽  
Ash Content ◽  
Chemical Extraction ◽  
Razor Clam ◽  
Degree Of Deacetylation ◽  
Randomized Design ◽  
Two Factors

Chitin can be found in animal source especially arthropods such as crustacean, mollusk and insect, as well as in plant source such as fungi. Chitosan is obtained from chitin after the acetyl group is removed from chitin structure through deacetylation step and has wide application in various fields (food, cosmetics and pharmaceutical). In this study, chitosan was extracted from razor clam shells, where the extraction conditions were optimized. Two- factors of randomized D-optimal design was used to determine the optimum condition for the extraction of chitosan from razor clam (Ensis arcuatus) by using response surface methodology (RSM). The chemical extraction was optimized using five levels with two factors which were the deacetylation time (2,4,6,8,10 hrs) and deacetylation temperature (50, 60, 70, 80 and 90ºC). A randomized design suggested by Design Expert software was implemented with four responses evaluated: yield (%); degree of deacetylation (%); molecular weight (kDA); and ash content (%). Time (h) and temperature (ºC) of the deacetylation significantly (p<0.05) affected the yield (%), degree of deacetylation (DDA) (%), molecular weight (Mw) (kDA) and ash content (%) of the chitosan extracted. The optimum conditions for the chitosan extraction were at the respective deacetylation time and temperature of 6h and 70ºC with actual values of yield (%), degree of deacetylation (%), molecular weight (kDA) and ash content (%) of 19.903±2.367, 50.113±0.902, 476.727±13.603, 8.517±2.094, respectively. The optimum condition for the chitosan extraction was experimentally verified and valid for further analysis.

Optimization of Extraction Technology of Favonoids from Kandelia Candel Leaves by Using Response Surface Methodology

2013 ◽  
Vol 704 ◽  
pp. 332-337
Author(s):  
Yan Ping Ji ◽  
Cheng Ping Li ◽  
Xing Xu ◽  
Lan Tang ◽  
Qian Tong
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Kandelia Candel ◽  
Liquid Ratio ◽  
Extraction Technology ◽  
Box Behnken Design ◽  
Extraction Parameters ◽  
Solid Liquid

This work is to extract flavonoids using ethanol refluxing method from the Kandelia candel leaves which are rich in flavonoids. Ethanol concentration, solid-liquid ratio, temperature and extraction time on the extraction rate were investigated by the single factor experiments. Box-Behnken design (BBD) based on response surface methodology (RSM) was adopted to optimize the parameters. The results showed the best extracting rate was more than 7.74%, when the extraction parameters were ethanol concentration 60%, extraction time 0.5 h and temperature 80°C.

Extraction and Partial Purification of Protease from Bilimbi (Averrhoa bilimbi L.)

2013 ◽  
Vol 10 (2) ◽  
pp. 29
Author(s):  
Normah Ismail ◽  
Nur' Ain Mohamad Kharoe
Keyword(s):  
Molecular Weight ◽  
Protein Content ◽  
Ammonium Sulfate ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Protease Activity ◽  
Protein Concentration ◽  
Temperature Stability ◽  
Partial Purification ◽  
Ammonium Sulfate Precipitation

Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of 40 and 60% (w/v). The collected proteases were analysed for pH, temperature stability, storage stability, molecular weight distribution, protein concentration and protein content. Protein content of bilimbi fruit was 0.89 g. Protease activity of both the unripe and ripe fruit were optimum at pH 4 and 40°C when the juice were purified at 40 and 60% ammonium sulfate precipitation. A decreased in protease activity was observed during the seven days of storage at 4°C. Molecular weight distribution indicated that the proteases protein bands fall between IO to 220 kDa. Protein bands were observed at 25, 50 and 160 kDa in both the unripe and ripe bilimbi proteases purified with 40% ammonium sulfate, however, the bands were more intense in those from unripe bilimbi. No protein bands were seen in proteases purified with 60% ammonium sulfate. Protein concentration was higher for proteases extracted with 40% ammonium sulfate at both ripening stages. Thus, purification using 40% ammonium sulfate precipitation could be a successful method to partially purify proteases from bilimbi especially from the unripe stage. 

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