Anti-salmonellosis agent for foodborne illness from Mangifera odorata (kuini) extracts

Food Research ◽  
2021 ◽  
Vol 5 (3) ◽  
pp. 262-272
Author(s):  
H. Adnan ◽  
N. Ismail ◽  
H. Hasan ◽  
M.S. Mat-Ali
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Antioxidant Power ◽  
Highly Active ◽  
Serovar Typhimurium ◽  
Bioassay Guided Fractionation ◽  
Ferric Reducing Antioxidant Power ◽  
Fractionation Method ◽  
Inhibition Performance

Salmonellosis infection caused by Salmonella bacteria is a public endemic problem in Malaysia with long-term morbidity and mortality effects. Thus, this study aimed to explore the antipathogenic activity of natural extracts from Mangifera odorata against two Salmonella species causing Salmonellosis. The extracts were derived from peel, flesh, and kernel seed of M. odorata. The inhibition performance of the extracts against both Salmonella enterica serovar Typhimurium and Salmonella enterica serovar Enteritidis bacteria were subsequently tested by using a bioassay-guided fractionation method. Results showed that the extracts derived from the kernel seed had the highest inhibition percentage of 83-90% against the Salmonellosis infection, followed by the peel with an inhibition of 61-67%, and lastly the flesh with an inhibition of 53-69%. The inhibition activities of hexane extracted flesh (FCH), methanol extracted peel (PCM), and methanol treated kernel seed (KTM) against S. enterica ser. Typhimurium bacteria were 59, 67 and 83%, respectively. Furthermore, the S. enterica ser. Enteritidis bacteria were found to be highly susceptible against the methanol extracted kernel seed (KCM), followed by the hexane extracted peel (PCH) and flesh (FTH) with the inhibition percentage of 90, 69 and 59%, respectively. The highly active anti-Salmonellosis performance of M. odorata extracts was attributed to its intrinsically high total phenolics content at 8-10 g GAE/g extract, high ferric reducing antioxidant power value (FRAP) at 18-22 g Fe2+/g extract and excellent scavenging activity with the inhibition performance ranges between 86% and 90%. This study revealed the antipathogenic activity of methanol extracts of M. odorata kernel seed inhibited the growth of both S. enterica ser. Typhimurium and S. enterica ser. Enteritidis bacteria. This study also discovered the prophylactic property of natural compounds in M. odorata kernel seed extracts and could be used as an anti-Salmonellosis agent. In the near future, M. odorata can be developed as an innovative functional food source for specific groups that are vulnerable to Salmonellosis

scholarly journals Potential Involvement of Salmonella Infection in Autoimmunity

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 96 ◽  
Author(s):  
Zhanna Ktsoyan ◽  
Lyudmila Budaghyan ◽  
Marina Agababova ◽  
Armine Mnatsakanyan ◽  
Karine Arakelova ◽  
...  
Keyword(s):  
Autoimmune Disease ◽  
Post Infection ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Profile Analysis ◽  
Infectious Agent ◽  
Disease Stage ◽  
Salmonella Infection ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Serovar Typhimurium

In this work, we investigated the potential effects of nontyphoidal Salmonella infection on autoantibody (AA) formation. The titer and profiles of autoantibodies in the sera of patients with acute salmonellosis due to Salmonella enterica serovar Typhimurium (S. Typhimurium) or Salmonella enterica serovar Enteritidis (S. Enteritidis) infection, as well as in convalescent patients, were determined with indirect immunofluorescence. A significant increase of autoantibodies in acute diseases caused by both serotypes of Salmonella and during post infection by S. Enteritidis was detected. Antibody profile analysis by multivariate statistics revealed that this increase was non-specific and was not dependent on the infectious agent or disease stage. The results obtained suggest that nontyphoidal Salmonella infection contributes to the generation of autoantibodies and may play a role in autoimmune disease.

scholarly journals Sensitivity of enrichment-PCR method for Salmonella enterica serovar Typhimurium and Salmonella enterica serovar Enteritidis analysis in chicken carcasses

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 54-61
Author(s):  
H.A. Wulan ◽  
Nurjanah S. ◽  
W.P. Rahayu
Keyword(s):  
Salmonella Enterica ◽  
Pathogenic Bacteria ◽  
Target Genes ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Salmonella Spp ◽  
High Prevalence ◽  
Serovar Typhimurium ◽  
Peptone Water ◽  
Pcr Method

Salmonella spp. is Gram negative-pathogenic bacteria that usually found as a contaminant in chicken carcasses. This study was aimed to increase the sensitivity of PCR enrichment step and apply the enrichment-PCR combination to detect Salmonella in chicken carcasses. In this study were used Salmonella enterica serovar Hadar, Salmonella enterica serovar Typhimurium and Salmonella enterica serovar Enteritidis with the target genes were invA, STM4497, and respectively. A total of 25 g of the chicken carcasses were artificially contaminated by approximately 0.96 and 3.33 MPN/mL for each serovar separately. Samples were incubated in pre-enrichment and enrichment media for 8 hrs prior to the DNA extraction. The pre-enrichment and enrichment media was Buffered Peptone Water and Rappaport-Vassiliadis-soya. The result showed that the target genes of S. enterica ser. Hadar, S. enterica ser. Typhimurium and S. enterica ser. Enteritidis were detected in chicken carcasses, indicated by the presence of DNA band with the size was 429 bp, 311 bp and 135 bp respectively. These result in line with analysis using ISO method and BLAST-comparison analysis of DNA amplicon sequences with GenBank references. Application of this method for Salmonella detection in chicken carcasses sold in the traditional market showed a higher prevalence than the previous result without enrichment. All samples (n = 100) from unsanitary practice sellers were positively contaminated by Salmonella spp. and also high prevalence for S. enterica ser. Typhimurium and S. enterica ser. Enteritidis. It can be concluded that enrichment is an important step to increase the sensitivity detection of PCR method.

scholarly journals Molecular characterization of Salmonella spp. isolates from river and dam water, irrigated vegetables, livestock, and poultry manures in Jordan

2021 ◽  
Vol 14 (3) ◽  
pp. 813-819
Author(s):  
Yaser H. Tarazi ◽  
Abdallah F. Al Dwekat ◽  
Zuhair Bani Ismail
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
High Morbidity ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Salmonella Spp ◽  
Potential Threat ◽  
Salmonella Enterica Serovar Typhi ◽  
Microbiological Methods ◽  
Serovar Typhimurium ◽  
Zarqa River

Background and Aim: Salmonellosis is an important food-borne and zoonotic disease with high morbidity and mortality rates. The objectives of this study were to isolate, serotype, and genetically characterize Salmonella spp. from Zarqa river and King Talal dam waters, vegetables irrigated by such waters, and manure of poultry and livestock farms located in the Zarqa river basin in Jordan. In addition, certain virulence factors and antimicrobial resistance patterns of isolated Salmonella strains were determined. Materials and Methods: A total of 250 samples were cultured using routine microbiological methods. Suspected Salmonella spp. were identified based on colony morphology and confirmed using biochemical and molecular methods. Virulence genes including invA, stn, and pCT plasmid were detected using multiplex PCR. Phylogenetic analysis was performed using pulsed-field gel electrophoresis (PFGE). Results: In total, 32/250 (12.8%) Salmonella spp. isolates were recovered from different sources. Of these, the most common serotype was Salmonella subspecies 1 (23 isolates), followed by Salmonella enterica serovar Typhimurium (4 isolates), Salmonella enterica serovar Typhi (3 isolates), and finally Salmonella enterica serovar Enteritidis (2 isolates). The PFGE indicated that Salmonella enterica serovar Typhimurium isolated from poultry manure and from parsley were closely related (84.6%). Salmonella enterica serovar Enteritidis isolated from the dam water was closely related to Salmonella enterica serovar Enteritidis isolated from spearmint (73.8%). Salmonella enterica serovar Typhi isolated from the river and dam water were 100% related to Salmonella enterica serovar Typhi isolated from lettuce. In the antimicrobial sensitivity test, 14 out of 32 (43.8%) isolated Salmonella strains were resistant to two or more of the major antimicrobial agent groups. However, the majority of isolates were sensitive to ceftriaxone, ciprofloxacin, cefuroxime, and gentamicin (97%, 93.8%, and 87.5%, 84.4%, respectively). All isolates were resistant to erythromycin and amoxicillin. Conclusion: Results of this study indicate a serious potential threat to public health associated with consuming leafy green vegetables grown on the banks of Zarqa river and its dam because of widespread Salmonella spp. contamination. Appropriate monitoring of irrigation water must be applied to reduce the possibility of cross-contamination.

Three Salmonella enterica serovar Enteritidis bacteriophages from the Siphoviridae family are promising candidates for phage therapy

2018 ◽  
Vol 64 (11) ◽  
pp. 865-875 ◽  
Author(s):  
Yibao Chen ◽  
Erchao Sun ◽  
Jiaoyang Song ◽  
Yigang Tong ◽  
Bin Wu
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Foodborne Pathogen ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Gastrointestinal Infections ◽  
The Family ◽  
Serovar Typhimurium ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Significant Health

Salmonella is a common and widely distributed foodborne pathogen that is frequently implicated in gastrointestinal infections. The emergence and spread of Salmonella strains resistant to multiple antibiotics poses a significant health threat, highlighting the urgent need for early and effective therapeutic strategies. We isolated a total of 32 phages from water samples and anal swabs from pigs. Of these, three phages that produced large, clear plaques were selected for further study using the following methods: electron microscopy, analysis of the life cycle parameters, genetic analysis, inhibition of bacterial growth, and activity against biofilms. The three Salmonella phages (vB_SenS_CSP01, vB_SenS_PHB06, and vB_SenS_PHB07) were assigned to the family Siphoviridae on the basis of their morphology. All showed polyvalent infectivity, and individual phages or phage cocktails could inhibit the growth of host Salmonella enterica serovar Enteritidis strains or reduce biofilm formation by Salmonella enterica serovar Typhimurium. In summary, these three phages merit further research as biocontrol agents for Salmonella infection.

scholarly journals Seroprevalence and Microbiological Monitoring in Eggs for Salmonella enterica Serovar Enteritidis and Salmonella enterica Serovar Typhimurium in Ornamental Chicken Flocks in Italy

2021 ◽  
Vol 41 (01) ◽  
pp. 39-44
Author(s):  
A Guerrini
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Public Health Problem ◽  
Serum Samples ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Salmonella Spp ◽  
Culture Methods ◽  
Serological Tests ◽  
Italian Regions ◽  
Serovar Typhimurium

Few data are available about the prevalence of Salmonella enterica serovar Enteritidis (S.E.) and Salmonella enterica serovar Typhimurium (S.T.) in ornamental poultry in Italy. The aim of this study was to investigate the seroprevalence for S.E. and S.T. using serological tests and the prevalence of Salmonella spp. in eggs by culture methods. For this purpose, 240 serum samples and 216 eggs were sampled from asymptomatic and unvaccinated ornamental hens reared in 24 farms, located in 8 different Italian regions. As screening test, a Tube Serum Agglutination test (TSA) was performed on 231 out of 240 serum samples. Four out of 24 farms (16.67%) were serologically positive for Salmonella spp. for a total of 10 samples. These positive samples were confirmed using an ELISA test and the results show that 5/231 (2.16%) and 7/231 (3.03%) serum samples were positive for S.E. and S.T. respectively, and 2/231 (0.87%) for both serotypes. Among all farms, 2/24 (8.33%) were positive for S.E. and 4/24 (16.67%) for S.T. The analysis of eggs using culture methods gave negative results for both yolk and shell pools (0/48, 0.0%). The seroconversion associated with exposure to S.E./S.T. in ornamental poultry, poses a potential public health problem. This study confirms that S.E. and S.T. are widespread in studied backyard poultry farms as asymptomatic form, and animals as potential reservoirs of Salmonella. It is necessary to inform farmers that a regular and periodic control of animals, eggs or meat, is very important to prevention of Salmonella foodborne infections and their spread.

scholarly journals Sugar sulfates are not hydrolyzed by the acid-inducible sulfatase AslA from Salmonella enterica Enteritidis NalR and Kentucky 3795 at pH 5.5

2017 ◽  
Vol 63 (8) ◽  
pp. 739-744
Author(s):  
Arpeeta Ganguly ◽  
Rolf D. Joerger
Keyword(s):  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Open Reading Frames ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Heparin Sulfate ◽  
Serovar Typhimurium ◽  
Type Strains ◽  
Reading Frames ◽  
Adhesion And Invasion ◽  
Invasion Assays

The open reading frames SEN0085 and SeKA_A4361, from Salmonella enterica serovar Enteritidis NalR and serovar Kentucky 3795, respectively, corresponding to the acid-inducible sulfatase gene aslA from Salmonella enterica serovar Typhimurium, were previously suggested by microarray analysis to be differentially expressed under acid conditions. However, growth and enzyme activity tests in the present study demonstrated that both wild-type strains exhibited sulfatase activity with 4-nitrophenyl sulfate and 5-bromo-4-chloro-3 indolyl sulfate at pH 5.5. The acid sulfatase does not appear to be involved in sugar sulfate, tyrosine sulfate, 4-hydroxy-3-methoxyphenylglycol sulfate, heparin sulfate, or chondroitin sulfate hydrolysis at pH 5.5. Adhesion and invasion assays did not reveal differences between the serotypes and their corresponding aslA deletion mutants. Thus, the role and substrate(s) of AslA, a protein unique to salmonella and encoded in all sequenced Salmonella strains, remain elusive.

scholarly journals Invisible mais dangereux, le portage bactérien commence à révéler ses secrets

2008 ◽  
Vol 21 (2) ◽  
Author(s):  
M. DUCHET-SUCHAUX
Keyword(s):  
Campylobacter Jejuni ◽  
Salmonella Enterica ◽  
Salmonella Enterica Serovar Typhimurium ◽  
Salmonella Enterica Serovar Enteritidis ◽  
Mise En Œuvre ◽  
Serovar Typhimurium ◽  
Animaux Domestiques

Le portage bactérien chez les animaux domestiques a commencé à être analysé relativement récemment, malgré son importance considérable dans l’épidémiologie et l’évolution des maladies infectieuses. La grande majorité des exemples documentés concernent des zoonoses, maladies transmises des animaux à l’Homme, le plus souvent via les aliments. L’animal porteur est infecté par un agent infectieux mais ne présente pas de symptômes consécutifs à cette infection ; cette bactérie est transmise à l’Homme ou à d’autres animaux qui eux, pourront développer une maladie, plus ou moins grave. La prévalence du portage chez les animaux est très élevée dans certains cas et son importance est aussi liée à la fréquence et à la gravité des maladies qu’il suscite. Il est mal compris et une meilleure compréhension de ses mécanismes devrait en améliorer la maîtrise, ce qui limitera les risques de maladie notamment chez l’Homme. La mise en œuvre d’outils d’analyse dans des modèles expérimentaux pertinents a permis de commencer à étudier les facteurs bactériens comme les facteurs de l’hôte impliqués dans le portage. Le plus documenté, l’exemple du portage intestinal a été comparé dans plusieurs modèles : ceux de Salmonella enterica sérovar Typhimurium ou Salmonella enterica sérovar Enteritidis chez la poule, de Campylobacter jejuni dans la même espèce et d’Escherichia coli O157:H7 chez les ruminants. Ce bilan met en évidence une multiplicité des facteurs en cause aussi bien chez la bactérie que chez l’hôte et il identifie des facteurs spécifiques à chaque modèle et quelques points communs. Il montre la mise en jeu de régulations ou de modulations contribuant à expliquer cet équilibre entre la bactérie et son hôte.

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