scholarly journals The molecular forms of BMP15 in a range of mammalian species

2021 ◽  
Author(s):  
◽  
Gene Swinerd
Keyword(s):  
Mammalian Species ◽  
Follicular Development ◽  
Conditioned Media ◽  
Molecular Forms ◽  
Cross Linking ◽  
Species Variation ◽  
Specific Effects ◽  
Fair Representation ◽  
Predominant Form ◽  
Species Specific

<p>BMP15 is an oocyte-secreted growth factor that is critical for ovarian follicular development and fertility in mammals. To improve our understanding of the species-specific effects of BMP15 on fertility, the aim of this study was to elucidate the molecular forms of BMP15 in mammals with high (rat and pigs) and low (sheep and deer) litter sizes. Western blotting experiments were undertaken using a monoclonal antibody directed against a semi-conserved area on the mature region of E.coli-expressed ovine BMP15.  Within the oocyte lysate of all species tested, the predominant form was promature BMP15, although mature BMP15 was also present. Within oocyte-conditioned media of sheep, pigs and deer, the promature:mature BMP15 ratio dropped significantly from what was seen inside the oocyte, with promature BMP15 only slightly predominant in deer, and relatively equal amounts of each protein were present in pigs and sheep. No BMP15 was detected in the oocyte-conditioned media of rats. Cross-linking studies did not provide any evidence of BMP15/BMP15 homodimers or BMP15/GDF9 heterodimers in any species tested.  Intra-oocyte levels of both promature and mature BMP15, from highest to lowest, were detected in deer, followed by sheep and pigs (similar), and then rats (significantly lower). In the oocyte-conditioned media, with the exception of the rat where BMP15 was absent, there was a similar pattern of promature BMP15 levels observed between species, whereas mature BMP15 levels showed no inter-species variation.  In summary, the molecular forms and relative amounts of BMP15 protein differ across species. Generally, high BMP15 levels were associated with low litter size; however the pig is an exception. Furthermore, the molecular forms observed in this study differed from those reported in studies using recombinant BMP15 suggesting that recombinantly-expressed protein may not provide a fair representation of native BMP15.</p>

scholarly journals The molecular forms of BMP15 in a range of mammalian species

2021 ◽  
Author(s):  
◽  
Gene Swinerd
Keyword(s):  
Mammalian Species ◽  
Follicular Development ◽  
Conditioned Media ◽  
Molecular Forms ◽  
Cross Linking ◽  
Species Variation ◽  
Specific Effects ◽  
Fair Representation ◽  
Predominant Form ◽  
Species Specific

<p>BMP15 is an oocyte-secreted growth factor that is critical for ovarian follicular development and fertility in mammals. To improve our understanding of the species-specific effects of BMP15 on fertility, the aim of this study was to elucidate the molecular forms of BMP15 in mammals with high (rat and pigs) and low (sheep and deer) litter sizes. Western blotting experiments were undertaken using a monoclonal antibody directed against a semi-conserved area on the mature region of E.coli-expressed ovine BMP15.  Within the oocyte lysate of all species tested, the predominant form was promature BMP15, although mature BMP15 was also present. Within oocyte-conditioned media of sheep, pigs and deer, the promature:mature BMP15 ratio dropped significantly from what was seen inside the oocyte, with promature BMP15 only slightly predominant in deer, and relatively equal amounts of each protein were present in pigs and sheep. No BMP15 was detected in the oocyte-conditioned media of rats. Cross-linking studies did not provide any evidence of BMP15/BMP15 homodimers or BMP15/GDF9 heterodimers in any species tested.  Intra-oocyte levels of both promature and mature BMP15, from highest to lowest, were detected in deer, followed by sheep and pigs (similar), and then rats (significantly lower). In the oocyte-conditioned media, with the exception of the rat where BMP15 was absent, there was a similar pattern of promature BMP15 levels observed between species, whereas mature BMP15 levels showed no inter-species variation.  In summary, the molecular forms and relative amounts of BMP15 protein differ across species. Generally, high BMP15 levels were associated with low litter size; however the pig is an exception. Furthermore, the molecular forms observed in this study differed from those reported in studies using recombinant BMP15 suggesting that recombinantly-expressed protein may not provide a fair representation of native BMP15.</p>

Diversity of Primary Structures of the Carboxy-terminal Regions of Mammalian Fibrinogen Aα-Chains

1993 ◽  
Vol 69 (04) ◽  
pp. 351-360 ◽  
Author(s):  
Masahiro Murakawa ◽  
Takashi Okamura ◽  
Takumi Kamura ◽  
Tsunefumi Shibuya ◽  
Mine Harada ◽  
...  
Keyword(s):  
Amino Acid ◽  
Rhesus Monkey ◽  
Syrian Hamster ◽  
Tandem Repeats ◽  
Mammalian Species ◽  
Amino Acid Sequences ◽  
Point Of View ◽  
Cross Linking ◽  
Amino Terminal ◽  
Rgd Sequence

SummaryThe partial amino acid sequences of fibrinogen Aα-chains from five mammalian species have been inferred by means of the polymerase chain reaction (PCR). From the genomic DNA of the rhesus monkey, pig, dog, mouse and Syrian hamster, the DNA fragments coding for α-C domains in the Aα-chains were amplified and sequenced. In all species examined, four cysteine residues were always conserved at the homologous positions. The carboxy- and amino-terminal portions of the α-C domains showed a considerable homology among the species. However, the sizes of the middle portions, which corresponded to the internal repeat structures, showed an apparent variability because of several insertions and/or deletions. In the rhesus monkey, pig, mouse and Syrian hamster, 13 amino acid tandem repeats fundamentally similar to those in humans and the rat were identified. In the dog, however, tandem repeats were found to consist of 18 amino acids, suggesting an independent multiplication of the canine repeats. The sites of the α-chain cross-linking acceptor and α2-plasmin inhibitor cross-linking donor were not always evolutionally conserved. The arginyl-glycyl-aspartic acid (RGD) sequence was not found in the amplified region of either the rhesus monkey or the pig. In the canine α-C domain, two RGD sequences were identified at the homologous positions to both rat and human RGD S. In the Syrian hamster, a single RGD sequence was found at the same position to that of the rat. Triplication of the RGD sequences was seen in the murine fibrinogen α-C domain around the homologous site to the rat RGDS sequence. These findings are of some interest from the point of view of structure-function and evolutionary relationships in the mammalian fibrinogen Aα-chains.

Density-dependent and species-specific effects on self-organization modulate the resistance of mussel bed ecosystems to hydrodynamic stress

2021 ◽  
Author(s):  
Gerardo I. Zardi ◽  
Katy Rebecca Nicastro ◽  
Christopher D. McQuaid ◽  
Monique de Jager ◽  
Johan van de Koppel ◽  
...  
Keyword(s):  
Self Organization ◽  
Mussel Bed ◽  
Hydrodynamic Stress ◽  
Density Dependent ◽  
Specific Effects ◽  
Species Specific

scholarly journals Modeling human brain tumours in flies, worms, and zebrafish: From proof of principle to novel therapeutic targets

2020 ◽  
Author(s):  
Uswa Shahzad ◽  
Michael S Taccone ◽  
Sachin A Kumar ◽  
Hidehiro Okura ◽  
Stacey Krumholtz ◽  
...  
Keyword(s):  
Brain Tumours ◽  
Mammalian Species ◽  
Scientific Investigation ◽  
Screening Tools ◽  
Murine Models ◽  
Molecular Processes ◽  
C Elegans ◽  
Human Brain Tumours ◽  
Species Specific ◽  
Cancer Studies

Abstract For decades, cell biologists and cancer researchers have taken advantage of non-murine species to increase our understanding of the molecular processes that drive normal cell and tissue development, and when perturbed, cause cancer. The advent of whole genome sequencing has revealed the high genetic homology of these organisms to humans. Seminal studies in non-murine organisms such as D. melanogaster, C. elegans, and D. rerio identified many of the signaling pathways involved in cancer. Studies in these organisms offer distinct advantages over mammalian cell or murine systems. Compared to murine models, these three species have shorter lifespans, are less resource intense, and are amenable to high-throughput drug and RNA interference screening to test a myriad of promising drugs against novel targets. In this review, we introduce species specific breeding strategies, highlight the advantages of modeling brain tumours in each non-mammalian species, and underscore the successes attributed to scientific investigation using these models. We conclude with an optimistic proposal that discoveries in the fields of cancer research, and in particular neuro-oncology, may be expedited using these powerful screening tools and strategies.

Variation in vegetation cover and seedling performance of tree species in a forest-savanna ecotone

2019 ◽  
Vol 35 (2) ◽  
pp. 74-82 ◽  
Author(s):  
Hamza Issifu ◽  
George K. D. Ametsitsi ◽  
Lana J. de Vries ◽  
Gloria Djaney Djagbletey ◽  
Stephen Adu-Bredu ◽  
...  
Keyword(s):  
Vegetation Cover ◽  
Seedling Recruitment ◽  
Seedling Survival ◽  
Tree Cover ◽  
First Year ◽  
Transplant Experiment ◽  
Tree Seedling ◽  
Savanna Woodland ◽  
Specific Effects ◽  
Species Specific

AbstractDifferential tree seedling recruitment across forest-savanna ecotones is poorly understood, but hypothesized to be influenced by vegetation cover and associated factors. In a 3-y-long field transplant experiment in the forest-savanna ecotone of Ghana, we assessed performance and root allocation of 864 seedlings for two forest (Khaya ivorensis and Terminalia superba) and two savanna (Khaya senegalensis and Terminalia macroptera) species in savanna woodland, closed-woodland and forest. Herbaceous vegetation biomass was significantly higher in savanna woodland (1.0 ± 0.4 kg m−2 vs 0.2 ± 0.1 kg m−2 in forest) and hence expected fire intensities, while some soil properties were improved in forest. Regardless, seedling survival declined significantly in the first-year dry-season for all species with huge declines for the forest species (50% vs 6% for Khaya and 16% vs 2% for Terminalia) by year 2. After 3 y, only savanna species survived in savanna woodland. However, best performance for savanna Khaya was in forest, but in savanna woodland for savanna Terminalia which also had the highest biomass fraction (0.8 ± 0.1 g g−1 vs 0.6 ± 0.1 g g−1 and 0.4 ± 0.1 g g−1) and starch concentration (27% ± 10% vs 15% ± 7% and 10% ± 4%) in roots relative to savanna and forest Khaya respectively. Our results demonstrate that tree cover variation has species-specific effects on tree seedling recruitment which is related to root storage functions.

Bifidobacteria exert species-specific effects on constipation in BALB/c mice

2017 ◽  
Vol 8 (10) ◽  
pp. 3587-3600 ◽  
Author(s):  
Linlin Wang ◽  
Lujun Hu ◽  
Qi Xu ◽  
Tian Jiang ◽  
Shuguang Fang ◽  
...  
Keyword(s):  
Specific Effects ◽  
Species Specific

Edible bifidobacteria exerted species-specific effects in relieving constipation.

scholarly journals Species-Specific Effects of Ant Inhabitants on Bromeliad Nutrition

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0152113 ◽  
Author(s):  
Ana Z. Gonçalves ◽  
Rafael S. Oliveira ◽  
Paulo S. Oliveira ◽  
Gustavo Q. Romero
Keyword(s):  
Specific Effects ◽  
Species Specific

Species-specific Effects of Nitromethylene Heterocycle Insecticides on Nicotinic Receptors in Locust Neurons and Mouse N1E-115 and BC3H1 Cells

1994 ◽  
Vol 22 (6) ◽  
pp. 454-461
Author(s):  
Marga Oortgiesen ◽  
Ruud Zwart ◽  
Henk P.M. Vijverberg
Keyword(s):  
Mammalian Cells ◽  
Neuroblastoma Cells ◽  
Receptor Subtypes ◽  
Inward Current ◽  
Specific Effects ◽  
Blocking Effects ◽  
Inward Currents ◽  
Species Specific ◽  
Ganglion Neurons

The effects of nitromethylene heterocycle (NMH) insecticides on subtypes of nicotinic acetylcholine (nACh) receptors were investigated in locust thoracic ganglion neurons, mouse N1E-115 neuroblastoma cells, and mouse BC3H1 muscle cells by using electrophysiological techniques. In locust neurons, all of the six NMH insecticides tested induced transient inward currents resembling nicotinic ACh-induced inward currents, while, in the continued presence of the NMH compounds, the ACh-induced inward current was blocked. The amplitude of the inward current and the blocking effects of the NMH insecticides were enhanced by concentrations between 0.1 and 10μM. Cross-desensitisation with the ACh-induced inward current confirmed that the NMH-induced inward current was governed by the activation of nACh receptors. Mammalian endplate type nACh receptors in BC3H1 cells and mammalian neuronal type nACh receptors in N1E-115 cells were much less sensitive to the NMH insecticides than the locust neuronal nACh receptors. At a concentration of 10μM, which blocked 80–100% of the ACh-induced inward current in locust neurons, NMH insecticides only partially blocked the ACh-induced inward currents mediated by the two subtypes of mammalian nACh receptors. NMH insecticides also failed to induce significant agonist effects in the mammalian cells at this concentration. The results provide a possible explanation for the selectively greater toxicity of NMH insecticides to insects than to vertebrates, at the level of nACh receptor subtypes and, hence, demonstrate that this in vitro approach is valuable for the investigation of species-specific interactions of compounds at their target site.

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