Clinical and Laboratory Assessment of the Effect on the Body of Rats of Biopolymer Based on Polylactide after Implantation in the Femur

2018 ◽  
Vol 3 (3) ◽  
pp. 30-33
Author(s):  
V. B. Makarov ◽  
◽  
D. V. Morozenko ◽  
F. S. Leontieva ◽  
◽  
...  
Keyword(s):  
The Body ◽  
Laboratory Assessment

Accuracy and Precision Study: Body Fluid White Blood Cell (WBC) Analysis (Peritoneal, Pleural and Peritoneal Dialysate) Using a Light Scatter Technology (ADVIA®2120/2120i) Versus Hemocytometer Manual Counts

Blood ◽  
2010 ◽  
Vol 116 (21) ◽  
pp. 4730-4730 ◽  
Author(s):  
Donna D Castellone ◽  
Ellinor I.B. Peerschke ◽  
Nenita Francisco ◽  
William Canfield ◽  
Gail Kling
Keyword(s):  
Blood Cell ◽  
White Blood Cell ◽  
Body Fluid ◽  
The Body ◽  
Light Scatter ◽  
Laboratory Assessment ◽  
Peritoneal Dialysate ◽  
Manual Method ◽  
Reproducibility Study

Abstract Abstract 4730 Background: The ADVIA® 2120/2120i body fluid application is an in vitro diagnostic test for the enumeration of the total nucleated cell (TNC) or white blood cell (WBC) count and RBC (red blood cell) count for pleural, peritoneal and peritoneal dialysis (PD) specimens collected in K2 EDTA. Total nucleated cell (TNC) and RBC counts are used in the laboratory assessment of these fluids. The TNC count is an absolute count of the nucleated blood cells, mesothelial cells, and other non-hematopoietic cells in these fluids. Objective: The objective is to demonstrate concordance between the manual (hemocytometer) method versus the light scatter principle used on the ADIVA® 2120i analyzer. Methods: The ADVIA® 2120/2120i Body Fluid Application uses the Basophil/Lobularity and RBC/PLT channels to enumerate the WBC and RBC counts. WBC counts are derived from the Basophil/Lobularity channel by a process in which the cytoplasmic membrane is stripped and the suspension of cells is intercepted by light from the laser diode where the low-angle light scatter (2° to 3°) and high-angle light scatter (5° to 15°) signals of each cell are counted. The manual method uses a hemocytometer in which a chamber is charged with fluid and both areas of the chamber are counted and the result averaged. De-identified remnant peritoneal, pleural and peritoneal dialysate samples were processed within 2 hours in the Hematology Laboratory according to their standard operating procedure. Samples with TNC counts that fell in the range of 0.030 to 400 × 103 cells/μ L (30 to 400,000) for each type of fluid were included. Ten consecutive runs were performed for each type of fluid to determine precision. Results: The WBC reproducibility study (n=10) demonstrates that within the run, precision of the body fluid counts demonstrates a coefficient of variation (CV) ≤ 10 CV. Regression analysis (n=34) demonstrates an r=.93 using samples across the reportable range. Conclusion: These results suggest that the light scatter technology used on the ADVIA® 2120/2120i gives both accurate and precise WBC determinations and compares very well to the manual method for determination of WBC analysis in peritoneal, peritoneal, dialysate, and pleural fluids. Utilizing this method will decrease turn-around time for this process, resulting in a faster time to result and ultimately better patient outcomes. Disclosures: Castellone: Siemens Healthcare: Employment. Canfield:Siemens: Employment. Kling:Siemens: Employment.

STUDY OF THE LOCAL ANTIOXIDANT SYSTEM IN RATS AFTER TOOTH EXTRACTION IN A COMPARATIVE EXPERIMENT

2020 ◽  
pp. 65-68
Author(s):  
Арпине Бронислави Антонян ◽  
Дмитрий Юрьевич Харитонов ◽  
Алик Эдикович Петросян ◽  
Эльвира Валерьевна Ветрова
Keyword(s):  
Tooth Extraction ◽  
White Male ◽  
Practical Importance ◽  
Daily Practice ◽  
Mucosal Healing ◽  
Oxidative Status ◽  
The Body ◽  
Male Rats ◽  
Laboratory Assessment ◽  
Hydrogen Water

Проблема недостаточной прогнозируемости развития осложнений после операции удаления зуба и заживления слизистой оболочки имеет актуальность в ежедневной практике врача стоматолога-хирурга. Использование белых крыс-самцов линии Вистар в качестве биомоделирования сложного удаления зуба позволяет провести визуальную и лабораторную оценку течения раневого процесса и оценить влияние растворов, ионизированных серебром, и водородной воды на местный оксидативный статус. Исследование прооксидантного и антиоксидантного состояния имеет важное практическое значение, поскольку позволяет оценить местные компенсаторные возможности организма. Состояние равновесия характерно для физиологической нормы, в то время как воспалительные процессы приводят к разности между этими системами. Статья посвящена осложнениям, возникающим в области удаленных зубов у лабораторных крыс. Изучали влияние физиологического раствора, ионизированного серебром, и водородную воду в качестве жидкости для орошения операционного поля и влияние данных растворов на местный оксидативный статус, а также течение раневого процесса и эпителизацию лунки. Полученные в ходе экспериментального исследования результаты подтверждают необходимость применения новых растворов для орошения оперируемого поля в процессе операции удаления зуба The problem of insufficient prognosticability of complications after tooth extraction and mucosal healing is relevant in the daily practice of a dentist-surgeon. The use of white male rats of the Wistar line as a biomodeling of complex tooth extraction allows for visual and laboratory assessment of the course of the wound process and to assess the effect of solutions ionized with silver and hydrogen water on the local oxidative status. The study of the Pro-oxidant and antioxidant state is of great practical importance, since it allows us to evaluate the local compensatory capabilities of the body. The state of equilibrium is characteristic of the physiological norm, while inflammatory processes lead to a difference between these systems. The article is devoted to complications that occur in the area of removed teeth in laboratory rats. We studied the effect of saline solution ionized with silver and hydrogen water as a liquid for irrigation of the operating field and the effect of these solutions on the local oxidative status, as well as the course of the wound process and epithelization of the well. The results obtained during the experimental study confirm the need to use new solutions for irrigation of the operated field during the operation of tooth extraction

scholarly journals SERUM LIPID PROFILE OF SPAYED AND NON-SPAYED FEMALE DOGS ASSOCIATED WITH THE BODY CONDITION SCORE

2015 ◽  
Vol 16 (2) ◽  
pp. 262-267 ◽  
Author(s):  
Patricia Almeida Ferreira ◽  
Sabrina de Oliveira Capella ◽  
Stephanie de Souza Theodoro ◽  
Samuel Rodrigues Felix ◽  
William Peres ◽  
...  
Keyword(s):  
Lipid Profile ◽  
Body Condition ◽  
Serum Lipid ◽  
Body Condition Score ◽  
Strong Association ◽  
The Body ◽  
Serum Lipid Profile ◽  
Laboratory Assessment ◽  
Condition Score ◽  
Post Menopausal

Neutering females may be associated to weight gain in dogs. In order to diagnose possible alterations, such as diabetes and hyperlipidemias, laboratory assessment of carbohydrate and lipid metabolism is important. The aim of this study was to verify glycemia and serum lipid profile in non-spayed and spayed female dogs, and associate these results with the body condition score. Thirty-two spayed (n=16) and non-spayed (n=16) female dogs had their blood collected for biochemical analysis, and were classified as to their body condition score (BCS). Nine had normal BCS, 10 were overweight, and 13 were obese. Little difference was observed among spayed and non-spayed dogs. Glycemia of spayed canine females is higher than that of non-spayed dogs, regardless of the body condition score. Likewise, total cholesterol levels are higher in non-spayed females. The HDL is higher in non-spayed obese dogs than in spayed dogs with the same body condition, this parameter did not differ in other weight groups. Contrary to what happens in post-menopausal women, spaying dogs does not seem to have such a strong effect on the metabolic parameters assessed. This study revealed unique results, where neither spaying nor weight group had a strong association with metabolic alterations.

scholarly journals THE IMPACT OF OVERWEIGHT AND OBESITY ON THE QUALITY OF LIFE IN CHILDREN WITH BRONCHIAL ASTHMA

2020 ◽  
Vol 73 (6) ◽  
pp. 1140-1144
Author(s):  
Anna V. Kupkina ◽  
Oleksandr P. Volosovets ◽  
Sergii P. Kryvopustov ◽  
Marija P. Prokhorova ◽  
Olena V. Mozyrska
Keyword(s):  
Quality Of Life ◽  
Bronchial Asthma ◽  
Rating Scale ◽  
Overweight And Obesity ◽  
The Body ◽  
Life Questionnaire ◽  
Laboratory Assessment ◽  
Significant Difference ◽  
The Impact

The aim is to investigate if the overweight (OW) and obesity (OB) impact on the quality of life (QOL) of children with bronchial asthma (BA). Materials and methods: The study included 73 children aged 7 – 17, with moderate BA. Depending on the body mass index, patients were divided into the three clinical groups: normal body weight (NW) – 30 children, OW – 28 children and OB – 15 children. QOL was conducted by the Pediatric Asthma Quality of Life Questionnaire (PAQLQ). The statistical processing was carried out using the IBM SPSS Statistics Base (version 22) and EZR version 1.32. Results were considered statistically significant when p<0.05. Results: Children with BA and OB had statistically lower QOL than children with BA and NW in all PAQLQ domains. The Kruskal-Wallis H-test revealed a statistically significant difference between the different weight groups of children with BA both for the general QOL: H(2) = 37.51, p<0.001and for each rating scale separately. Pairwise comparisons using Steel-Dwass test indicated that scores of NW were observed to be significantly different from those of OW and OB for rating Activity and Symptoms scales (p<0.001). Conclusion: Comorbid OW and OB reduce the specific QOL of children with BA. In assessing the effectiveness of specific approaches to treating BA in children with OW and OB, an assessment of the QOL of children should be added to the traditional common clinical and laboratory assessment.

Representing utility and deploying the body

2020 ◽  
Vol 43 ◽  
Author(s):  
David Spurrett
Keyword(s):  
Functional Activity ◽  
Degrees Of Freedom ◽  
The Body ◽  
Target Article ◽  
Processing Demands ◽  
The Many

Abstract Comprehensive accounts of resource-rational attempts to maximise utility shouldn't ignore the demands of constructing utility representations. This can be onerous when, as in humans, there are many rewarding modalities. Another thing best not ignored is the processing demands of making functional activity out of the many degrees of freedom of a body. The target article is almost silent on both.

Neuron-glia relationship during the early postembryonic development of the nervous system in some oligochaetes

1978 ◽  
Vol 36 (2) ◽  
pp. 600-601
Author(s):  
Wiktor Djaczenko ◽  
Carmen Calenda Cimmino
Keyword(s):  
Nervous System ◽  
Glial Cells ◽  
Early Period ◽  
Postembryonic Development ◽  
Ruthenium Red ◽  
The Body ◽  
Tubifex Tubifex ◽  
Growth Stages ◽  
Cell Processes ◽  
Cytoplasmic Processes

The simplicity of the developing nervous system of oligochaetes makes of it an excellent model for the study of the relationships between glia and neurons. In the present communication we describe the relationships between glia and neurons in the early periods of post-embryonic development in some species of oligochaetes.Tubifex tubifex (Mull. ) and Octolasium complanatum (Dugès) specimens starting from 0. 3 mm of body length were collected from laboratory cultures divided into three groups each group fixed separately by one of the following methods: (a) 4% glutaraldehyde and 1% acrolein fixation followed by osmium tetroxide, (b) TAPO technique, (c) ruthenium red method.Our observations concern the early period of the postembryonic development of the nervous system in oligochaetes. During this period neurons occupy fixed positions in the body the only observable change being the increase in volume of their perikaryons. Perikaryons of glial cells were located at some distance from neurons. Long cytoplasmic processes of glial cells tended to approach the neurons. The superimposed contours of glial cell processes designed from electron micrographs, taken at the same magnification, typical for five successive growth stages of the nervous system of Octolasium complanatum are shown in Fig. 1. Neuron is designed symbolically to facilitate the understanding of the kinetics of the growth process.

Flagellar Attachment in Selected Trypanosomes

1973 ◽  
Vol 31 ◽  
pp. 496-497
Author(s):  
J. J. Paulin
Keyword(s):  
Cell Membrane ◽  
Lateral Surface ◽  
The Body ◽  
Flagellar Pocket ◽  
Integral Component ◽  
Free Flagellum ◽  
Flagellar Axoneme

Movement in epimastigote and trypomastigote stages of trypanosomes is accomplished by planar sinusoidal beating of the anteriorly directed flagellum and associated undulating membrane. The flagellum emerges from a bottle-shaped depression, the flagellar pocket, opening on the lateral surface of the cell. The limiting cell membrane envelopes not only the body of the trypanosome but is continuous with and insheathes the flagellar axoneme forming the undulating membrane. In some species a paraxial rod parallels the axoneme from its point of emergence at the flagellar pocket and is an integral component of the undulating membrane. A portion of the flagellum may extend beyond the anterior apex of the cell as a free flagellum; the length is variable in different species of trypanosomes.

Otoconia formation in the chick embryo

1983 ◽  
Vol 41 ◽  
pp. 572-573
Author(s):  
C.D. Fermin ◽  
M. Igarashi
Keyword(s):  
Chick Embryo ◽  
Inner Ear ◽  
Gallus Domesticus ◽  
The Body ◽  
Abnormal Behavior ◽  
Intensive Study ◽  
Basic Fuchsin ◽  
Gestational Period ◽  
Sensory Epithelia ◽  
Transmission Electron

Otoconia are microscopic geometric structures that cover the sensory epithelia of the utricle and saccule (gravitational receptors) of mammals, and the lagena macula of birds. The importance of otoconia for maintanance of the body balance is evidenced by the abnormal behavior of species with genetic defects of otolith. Although a few reports have dealt with otoconia formation, some basic questions remain unanswered. The chick embryo is desirable for studying otoconial formation because its inner ear structures are easily accessible, and its gestational period is short (21 days of incubation).The results described here are part of an intensive study intended to examine the morphogenesis of the otoconia in the chick embryo (Gallus- domesticus) inner ear. We used chick embryos from the 4th day of incubation until hatching, and examined the specimens with light (LM) and transmission electron microscopy (TEM). The embryos were decapitated, and fixed by immersion with 3% cold glutaraldehyde. The ears and their parts were dissected out under the microscope; no decalcification was used. For LM, the ears were embedded in JB-4 plastic, cut serially at 5 micra and stained with 0.2% toluidine blue and 0.1% basic fuchsin in 25% alcohol.

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