scholarly journals Biochemical Markers of Connective Tissue in the Laboratory Diagnosis of Orthopedic Human Diseases

2020 ◽  
Vol 5 (4) ◽  
pp. 167-172
Author(s):  
F. S. Leontieva ◽  
◽  
D. V. Morozenko ◽  
M. P. Vorontsova ◽  
K. V. Gliebova ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Biochemical Markers ◽  
Laboratory Diagnosis ◽  
Human Diseases

scholarly journals BIOCHEMICAL MARKERS OF CONNECTIVE TISSUE METABOLISM IN RATS’ BLOOD SERUM WITH EXPERIMENTAL KNEE OSTEOARTHRITIS AND THEIR DYNAMICS DURING CONSERVATIVE TREATMENT

2019 ◽  
Vol 72 (2) ◽  
pp. 193-197
Author(s):  
Kyrylo V. Makolinets ◽  
Vasyl I. Makolinets ◽  
Dmytro V. Morozenko ◽  
Kateryna V. Gliebova ◽  
Svitlana I. Danylchenko
Keyword(s):  
Laser Radiation ◽  
Conservative Treatment ◽  
Connective Tissue ◽  
Biochemical Markers ◽  
Diclofenac Sodium ◽  
Infrared Laser ◽  
Knee Joints ◽  
Glucosamine Hydrochloride ◽  
Tissue Metabolism ◽  
Connective Tissue Metabolism

Introduction: There are many methods for diagnosis and treatment of knee joints osteoarthritis in modern orthopedics. The infrared radiation is one of the most popular and effective methods though it needs experimental substantiation on rats. The aim: Experimental definition of the effectiveness of the proposed method of conservative treatment of knee joints osteoarthritis using sodium diclofenac, glucosamine hydrochloride and infrared laser radiation, on the basis of biochemical markers of connective tissue metabolism in rats. Materials and methods: Experimental studies were performed on 40 white male rats. A part of rats received infrared laser radiation in addition to diclofenac sodium and glucosamine hydrochloride. Results: Using a complex of infrared laser radiation and glucosamine hydrochloride and diclofenac sodium in conservative treatment of rats with experimentally induced knee joints osteoarthritis rats after 24 days of treatment. Treatment with the above-mentioned scheme resulted in a significant reduction in the content of glycoproteins (28.5%), chondroitin sulfates (26.1%), alkaline phosphatase activity (32.3%), and I and II fractions of glycosaminoglycans, namely chondroitin-6- (28.7%) and chondroitin-4-sulfate (40.3%). Moreover, these indicators of the studied markers approached the level of intact animals. Conclusions: Changes in biochemical markers of connective tissue in rats during experimental knee joints osteoarthritis indicated a higher efficiency of the treatment regimen with the use of low-intensity infrared laser radiation compared with exclusively drug therapy.

scholarly journals DYNAMICS OF BIOCHEMICAL MARKERS OF CONNECTIVE TISSUE METABOLISM IN PATIENTS WITH KNEE OSTEOARTHRITIS DURING CONSERVATIVE TREATMENT WITH LASER THERAPY

2019 ◽  
Vol 72 (5) ◽  
pp. 802-806
Author(s):  
Kyrylo V. Makolinets ◽  
Vasyl I. Makolinets ◽  
Dmytro V. Morozenko ◽  
Kateryna V. Gliebova ◽  
Svitlana I. Danylchenko
Keyword(s):  
Conservative Treatment ◽  
Connective Tissue ◽  
Knee Osteoarthritis ◽  
Blood Serum ◽  
Laser Therapy ◽  
Biochemical Markers ◽  
Diclofenac Sodium ◽  
Glucosamine Hydrochloride ◽  
Tissue Metabolism ◽  
Connective Tissue Metabolism

Introduction: The biochemical markers of the connective tissue metabolism in blood serum may characterize the disturbances in inflammatory, destructive processes, and also serve the criteria for diagnostic evaluation of the state of cartilage and bone tissue of the joints in knee osteoarthritis to control the effectiveness of conservative treatment. The aim: determination of biochemical markers of connective tissue metabolism in the blood of patients with the 1st and 2nd stages of knee osteoarthritis in order to prove the effectiveness of the complex conservative treatment of patients with the use of low-intensity infrared laser radiation. Materials and methods: During the study we examined 80 patients with the 1st and 2nd stages of knee osteoarthritis according to the Kellgren and Lawrence classification. Some patients were prescribed diclofenac sodium and glucosamine hydrochloride, others were prescribed an additional course of laser therapy. Results: At the end of the treatment the group of patients with knee osteoarthritis who were treated with the use of laser therapy, diclofenac sodium and glucosamine hydrochloride, showed a decrease in the content of the following blood serum components: glycoproteins (by 28.9 %), sialic acids (by 28.8 %), chondroitinsulfates (by 91.3 %) due to chondroitin-6-sulfate, activity of alkaline phosphatase (by 36.1 %) compared with the indicators before treatment. Conclusions: a more significant decrease in the inflammation and destruction of the cartilage tissue of the affected joints was observed during knee osteoarthritis treatment with the use of laser therapy than without it.

scholarly journals THE ROLE OF UNDIFFERENTIATED DYSPLASIA OF THE CONNECTIVE TISSUE IN THE DEVELOPMENT OF THE ENTEROCUTANEOUS FISTULA

2019 ◽  
pp. 5-10
Author(s):  
O.Yu. Usenko ◽  
Ya.Yu. Voitiv
Keyword(s):  
Connective Tissue ◽  
Biochemical Markers ◽  
Enterocutaneous Fistula ◽  
Prospective Trial ◽  
Prognostic Sign ◽  
Colocutaneous Fistula ◽  
Surgical Tactics ◽  
Severe Degree

Summary. Retro- and prospective trial was based on data about 30 patients, suffering entero-colocutaneous fistula, who were treated in the Shalimov National Institute of Surgery and Transplantology during 2016-2019.  There was revealed, that the most informative phenotypical markers of undifferentiated dysplasia of the connective tissue (UDCT) in patients, with entero-colocutaneous fistula, are visceral (83,3%), vascular (70%), arrhythmic (70%) syndromes. There was founded, that direct correlation between the level of biochemical markers of the collagen biodegradation and the UDCT degree may be applied for prognostication of development and course of complications in patients, suffering entero-colocutaneous fistula. Severe degree of UDCT in the patients, entero-colocutaneous fistula, constitutes unfavorable prognostic sign and enhances the mortality by 62.5%. The presence of UDCT in the patients winh entero-colocutaneous fistula is an aggravating comorbid factor that is difficult to be treated and accompanied by high rates of lethality, which must be taken into account, choosing the adequate surgical tactics and complex pathogenetically substantiated treatment.

scholarly journals Biochemical markers of connective tissue metabolism in the diagnostics of respiratory diseases in human and animals: retrospective analysis (1984–2010)

2021 ◽  
pp. 30-35
Author(s):  
Dmytro Morozenko ◽  
Roman Dotsenko ◽  
Yevheniia Vashchyk ◽  
Andriy Zakhariev ◽  
Andrii Zemlianskyi ◽  
...  
Keyword(s):  
Veterinary Medicine ◽  
Connective Tissue ◽  
Bronchoalveolar Lavage ◽  
Respiratory System ◽  
Biochemical Markers ◽  
Respiratory Diseases ◽  
Diagnostic Information ◽  
Source Analysis ◽  
Tissue Metabolism ◽  
Connective Tissue Metabolism

The aim: to analyze the literature data for the period from 1984 to 2010 on the use of biochemical markers of disorders of connective tissue metabolism in diseases of the respiratory system in humans and animals. Materials and methods. The research was conducted by the method of scientific literature open source analysis: PubMed, Elsevier, electronic resources of the National Library named after V.I. Vernadsky (1984–2010). Results. In the case of diseases of the respiratory system in humans, the pathogenesis of pneumonia is the development of inflammation in the interstitial, peribronchial, perivascular and perilobular connective tissue, lymphatic vessels of the lungs, followed by involvement of alveoli and bronchioles in the inflammation. The morphological basis of these changes may be pneumofibrosis and pneumosclerotic changes. In the chronic course of pneumonia, chronic obstructive pulmonary disease develops. This pathology is closely related to the action of inflammatory cytokines that regulate connective tissue proliferation. Similar studies were performed on eosinophilic bronchopneumonia in dogs, but the material for the study was bronchoalveolar lavage. The current method of diagnosing respiratory diseases using cytokines (interleukin-4, interferon-γ) and bronchoalveolar lavage has no diagnostic information in chronic bronchitis and bronchial asthma in cats. Fundamental studies of connective tissue biopolymers in clinically healthy and bronchopneumonia piglets have recently been conducted in veterinary medicine. Conclusions. Recently, in medicine of particular interest to researchers is the determination of the content in biological fluids of indicators of connective tissue metabolism (hydroxyproline, glycosaminoglycans, glycoproteins, sialic acids) to diagnose diseases of the respiratory system. To diagnose connective tissue disorders in lung diseases in medical practice use indicators of oxyproline in serum and urine. Oxyproline is one of the most important components of lung collagen. An increase in the content of free oxyproline in the blood indicates an increased rate of collagen breakdown in the lung tissue. Analysis of oxyproline fractions, as indicators of the direction of collagen metabolism, allows to assess the condition of the connective tissue of the lungs and can serve as a prognostic criterion for the course of the disease. Thus, the indicators of connective tissue metabolism showed significant diagnostic information, which allowed to recommend them for use in the practice of veterinary medicine.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Experimental Amyloidosis Induced by Immune Complex

1971 ◽  
Vol 29 ◽  
pp. 582-583
Author(s):  
A. Kawaoi
Keyword(s):  
Immune Complex ◽  
Systemic Amyloidosis ◽  
Human Diseases ◽  
Experimental Amyloidosis ◽  
Freund’S Complete Adjuvant ◽  
Freund's Complete Adjuvant ◽  
Immunological Approach ◽  
Experimentally Induced

Numbers of immunological approach have been made to the amyloidosis through the variety of predisposing human diseases and the experimentally induced animals by the greater number of agents. The results suggest an important role of impaired immunity involving both humoral and cell-mediated aspects.Recently the author has succeeded in producing amyloidosis in the rabbits and mice by the injections of immune complex of heat denatured DNA.The aim of this report is to demonstrate the details of the ultrastructure of the amyloidosis induced by heterologous insoluble immune complex. Eleven of twelve mice, dd strain, subcutaneously injected twice a week with Freund's complete adjuvant and four of seven animals intraperitonially injected developed systemic amyloidosis two months later from the initial injections. The spleens were electron microscopically observed.

Ultrastructure of Liver in Lactosyl Ceramidosis

1971 ◽  
Vol 29 ◽  
pp. 312-313
Author(s):  
Z. Hruban ◽  
J. R. Esterly ◽  
G. Dawson ◽  
A. O. Stein
Keyword(s):  
Connective Tissue ◽  
Liver Biopsy ◽  
Osmium Tetroxide ◽  
Close Contact ◽  
Multivesicular Bodies ◽  
Bile Canaliculi ◽  
Dense Bodies ◽  
Marginal Plates ◽  
Membranous Material ◽  
Perichromatin Granules

Samples of a surgical liver biopsy from a patient with lactosyl ceramidosis were fixed in paraformaldehyde and postfixed in osmium tetroxide. Hepatocytes (Figs. 1, 2) contained 0.4 to 2.1 μ inclusions (LCI) limited by a single membrane containing lucid matrix and short segments of curved, lamellated and circular membranous material (Fig. 3). Numerous LCI in large connective tissue cells were up to 11 μ in diameter (Fig. 2). Heterogeneous dense bodies (“lysosomes”) were few and irregularly distributed. Rough cisternae were dilated and contained smooth vesicles and surface invaginations. Close contact with mitochondria was rare. Stacks were small and rare. Vesicular rough reticulum and glycogen rosettes were abundant. Smooth vesicular reticulum was moderately abundant. Mitochondria were round with few cristae and rare matrical granules. Golgi complex was seen rarely (Fig. 1). Microbodies with marginal plates were usual. Multivesicular bodies were very rare. Neutral lipid was rare. Nucleoli were small and perichromatin granules were large. Small bile canaliculi had few microvilli (Fig. 1).

Ultrastructure of Lymphatic Capillaries in the Transport of Colloidal Particles

1967 ◽  
Vol 25 ◽  
pp. 186-187
Author(s):  
L. V. Leak ◽  
J. F. Burke
Keyword(s):  
Connective Tissue ◽  
Colloidal Particles ◽  
Ultrastructural Level ◽  
Vital Role ◽  
Time Intervals ◽  
Thorium Dioxide ◽  
Lymphatic Capillary ◽  
Tissue Area ◽  
Rapid Removal ◽  
Tissue Fluids

The vital role played by the lymphatic capillaries in the transfer of tissue fluids and particulate materials from the connective tissue area can be demonstrated by the rapid removal of injected vital dyes into the tissue areas. In order to ascertain the mechanisms involved in the transfer of substances from the connective tissue area at the ultrastructural level, we have injected colloidal particles of varying sizes which range from 80 A up to 900-mμ. These colloidal particles (colloidal ferritin 80-100A, thorium dioxide 100-200 A, biological carbon 200-300 and latex spheres 900-mμ) are injected directly into the interstitial spaces of the connective tissue with glass micro-needles mounted in a modified Chambers micromanipulator. The progress of the particles from the interstitial space into the lymphatic capillary lumen is followed by observing tissues from animals (skin of the guinea pig ear) that were injected at various time intervals ranging from 5 minutes up to 6 months.

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