Impact of the impulse electrophoresis of the blood plasma on the wounds healing and formation of cicatricial tissue in experiment

Objective. To study in experiment the impact of the impulse electrophoresis of the blood plasma method on the wounds healing process in postoperative wounds. Materials and methods. The investigations were conducted on 15 white rats, to which in aseptic environment under intraabdominal narcosis the hip surgical access through the skin and muscles was conducted with subsequent layered closure of the wound, using separate knots. Ten laboratory animals were included into the main group, in whom transdermal introduction of the blood plasma, using the impulse electrophoresis method, was conducted on the 4th, 7th, 10th and 14th postoperative days. Into a control group 5 white rats were included, in which transdermal introduction of the blood plasma was not applied postoperatively. In all the laboratory animals the wounds have healed primarily. On the19th day postoperatively the laboratory animals were extracted from the experiment, and the near suture skin was investigated, using histological methods. Results. The surgical access healing in the hip skin and muscles with formation of normotrophic cicatrices was observed in the main group of the laboratory animals. Hypertrophic cicatrix in the operative intervention zone have been formatted in the laboratory animals of a control group on the 19th postoperative day. Conclusion. More qualitative healing of the wounds with formation of normotrophic cicatrix while application of transdermal introduction of the blood plasma, using the impulse electrophoresis method, was confirmed in experimental investigation in laboratory animals of the main group. Excessive creation of collagen was omitted in the laboratory animals of the main group, what have promoted formation of more thin and tender cicatrix, which have become slightly visible up to the end of experiment.

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TOXICOLOGICAL PROFILE OF “BIOSOLBI” DRUG

Vestnik of the Russian agricultural science ◽

10.30850/vrsn/2020/1/67-69 ◽

1970 ◽

pp. 67-69

Author(s):

E. K. Rakhmatullin ◽

O. D. Sklyarov

Keyword(s):

Drug Toxicity ◽

Probit Analysis ◽

Laboratory Animals ◽

Control Group ◽

Hazard Class ◽

Water Ingestion ◽

White Rats ◽

Poorly Soluble ◽

And Behavior ◽

Purebred Dogs

The article presents the results of a study of the "Bisolbi" drug toxicity (powder of light ash color, poorly soluble in water). When it is mixed with water it forms a suspension of particles that settle rapidly. Values of acute drug toxicity were determined on rats. We studied groups of six animals of the same sex, as well as similar control ones. The "Bisolbi" drug was injected to white rats intragastrically, males weighing 310 ... 320 g in doses of 2500 and 2740 mg / kg. Each dose was used in six animals; distilled water (3 ml) was used for the controls. The LD50 was calculated by the probit analysis method proposed by Litchfield and Wilcoxon modified by Z. Roth. When administered orally, an atraumatic metal probe was immersed in the stomach. Within 14 days monitored the overall health status and behavior of animals, the manifestation or absence of symptoms of intoxication; noted the features of feed and water ingestion, assessed the condition of the coat, physiological functions. Then groups of experimental rats were euthanized and pathomorphologically examined. We studied the effect of "Bisolbi" with repeated introduction and on not purebred dogs. Two groups of 3-4 years of age were completed with an average initial body weight of 13.63 ... 15.11 kg. Before use, the additive was thoroughly mixed with feed. The drug was injected during 31 days at a dose of 0.5 g / kg. Dogs of the control group (three) were fed wheat flour. After 15 and 31 days in laboratory animals in order to characterize the general condition in the blood, the amount of protein, urea, glucose, creatinine, cholesterol were determined. Based on studies it was found that the drug daily application by animals, is low toxic and safe, does not provoke the development of pathological reactions. According to the Hodge and Sterner classification "Bisolbi" can be attributed to the 6th class of toxicity - relatively harmless. Accordingto GOST 12.1.007-76 LD50 of the drug is more than 151 mg / kg, but less than 5000 mg / kg it is the 3rd hazard class (moderately hazardous).

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Great Obstetrical Syndromes In G20210a Mutation Carriers Associated With High Prothrombin Activity

10.21203/rs.3.rs-67776/v1 ◽

2020 ◽

Author(s):

Mariya Nikolaeva ◽

Andrey Pavlovich Momot ◽

Marina Sabirovna Zainulina ◽

Natalia Nikolaevna Yasafova ◽

Irina Alekseevna Taranenko

Keyword(s):

Blood Plasma ◽

Venous Blood ◽

Threshold Value ◽

Main Group ◽

Trophoblast Invasion ◽

Control Group ◽

G20210a Mutation ◽

Factor Ii ◽

Invasion Waves ◽

In Blood Plasma

Abstract Objective: to study the association between high activity of Factor II (prothrombin) in blood plasma with G20210A mutation and the development of great obstetrical syndromes.Material and methods: A prospective clinical cohort study was conducted on 290 pregnant women (average age 31.7±4.7 years old). The main group was made up of 140 G20210A patients, while the control group comprised 150 women with the wild G20210G type. The aim was to evaluate the activity of Factor II in the venous blood plasma during the stages of pregnancy with regard to trophoblast invasion waves. As per results, association analysis of Factor II activity value and gestational complications was carried out.Results: In the control group, the median (Me) of Factor II activity ranged from 108% (preconception period) to 144% (pregnancy) [95% CI 130-150]. In patients with the GA type, the value was significantly higher in related periods, ranging from 149% to 181% [95% CI 142-195], p<0.0001. With Factor II activity ranging from 148.5% to 180.6%, pregnancies in the main group had no complications. Higher levels of Factor II activity were associated with the development of early and/or severe preeclampsia (PE) and fetal growth retardation (FGR).Conclusion: The data obtained regarding Factor II activity in blood plasma, juxtaposed with the development of great obstetrical syndromes, allow to assume that manifestation of G20210A in early and/or severe PE and FGR is associated with this coagulation factor's level of activity. Threshold value of the Factor II activity with G20210A mutation, allowing to predict the development of PE, comprised 171.0% at the preconception stage (AUC – 0.86; p<0.0001) and within 7-8 weeks of gestation it was 181.3% (AUC – 0.84; p<0.0001).

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Impact of Ellagic Acid in Bone Formation after Tooth Extraction: An Experimental Study on Diabetic Rats

The Scientific World JOURNAL ◽

10.1155/2014/908098 ◽

2014 ◽

Vol 2014 ◽

pp. 1-14 ◽

Cited By ~ 8

Author(s):

Mazen M. Jamil Al-Obaidi ◽

Fouad Hussain Al-Bayaty ◽

Rami Al Batran ◽

Jamal Hussaini ◽

Goot Heah Khor

Keyword(s):

Normal Saline ◽

Ellagic Acid ◽

Tooth Extraction ◽

Healing Process ◽

Diabetic Rats ◽

Male Rats ◽

Diabetic Rat ◽

Control Group ◽

Tooth Socket ◽

The Impact

Objectives. To estimate the impact of ellagic acid (EA) towards healing tooth socket in diabetic animals, after tooth extraction.Methods. Twenty-fourSprague Dawleymale rats weighing 250–300 g were selected for this study. All animals were intraperitoneally injected with 45 mg/kg (b.w.) of freshly prepared streptozotocin (STZ), to induce diabetic mellitus. Then, the animals were anesthetized, and the upper left central incisor was extracted and the whole extracted sockets were filled with Rosuvastatin (RSV). The rats were separated into three groups, comprising 8 rats each. The first group was considered as normal control group and orally treated with normal saline. The second group was regarded as diabetic control group and orally treated with normal saline, whereas the third group comprised diabetic rats, administrated with EA (50 mg/kg) orally. The maxilla tissue stained by eosin and hematoxylin (H&E) was used for histological examinations and immunohistochemical technique. Fibroblast growth factor (FGF-2) and alkaline phosphatase (ALP) were used to evaluate the healing process in the extracted tooth socket by immunohistochemistry test.Results. The reactions of immunohistochemistry for FGF-2 and ALP presented stronger expression, predominantly in EA treated diabetic rat, than the untreated diabetic rat.Conclusion. These findings suggest that the administration of EA combined with RSV may have accelerated the healing process of the tooth socket of diabetic rats, after tooth extraction.

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Impact of long-term supplementation of zinc and selenium on their content in blood and hair in goats

Veterinární Medicína ◽

10.17221/1581-vetmed ◽

2011 ◽

Vol 56 (No. 2) ◽

pp. 63-74 ◽

Cited By ~ 13

Author(s):

L. Pavlata ◽

M. Chomat ◽

A. Pechova ◽

L. Misurova ◽

R. Dvorak

Keyword(s):

Blood Plasma ◽

Whole Blood ◽

Average Concentration ◽

The Other ◽

Control Group ◽

Selenium Yeast ◽

Hair Samples ◽

The Impact ◽

Se Status

This paper evaluates the impact of long-term supplementation of different forms of zinc (Zn) and selenium (Se) on the content of these substances in the blood and hair of goats. Two analogous supplementation experiments were performed. 37 goats divided into four groups were used in the first trial with the Zn supplementation. Group A (n = 10) was a control group (with no Zn administered). A further three groups (B, C, D) were supplemented with Zn in various forms. Group B (n = 9) with zinc oxide, Group C (n = 9) with zinc lactate and Group D (n = 9) with zinc chelate. The second trial with Se supplementation was carried out on 20 goats divided into four groups. Group E (n = 5) was a control group. The other three groups were administered Se. Group F (n = 5) was supplied with a selenium lactate-protein complex, Group G (n = 5) with sodium selenite and Group H (n = 5) with selenium yeast. Three months later blood and hair samples were taken from all animals and Zn and Se concentrations were determined in whole blood, plasma, and hair. Glutathione peroxidase (GSH-Px) activity was determined in the Se supplementation trial group. At the end of the trial the Zn concentrations in plasma and whole blood were without major differences between the groups. The plasma concentration of Zn did not increase from the initial value at the start of the trial. In hair the average concentration of Zn was 95.2–100.0 mg/kg<br />in all groups. No conclusive relation was confirmed between the values of Zn in hair and its concentration in blood. The Se concentration in whole blood (µg/l) at the end of trial in supplemented groups (F – 188.8 ± 24.6; G – 197.2 ± 10.9; H – 190.1 ± 26.3) was significantly higher (P < 0.01) than in the control group (E – 103.1 ± 23.5). Similarly, the activity of GSH-Px (µkat/l) was significantly higher in all supplemented groups (F – 872.3 ± 94.8; G – 659.5 ± 176.4; H – 839.8 ± 150.8) than in the control group (E – 379.1 ± 63.5). Se content in hair (µg/kg) was higher also in all trial groups (F – 242.3 ± 41.5; G – 200.5 ± 46.9; H – 270.0 ± 106.8) than in the control group (E – 174.7 ± 38.0). However, it was significantly (P < 0.05) higher only in Group F. A conclusive correlation was identified between the Se concentration in whole blood and its content in hair (r = 0.54; P < 0.05; n = 20). Based on the results it can be concluded that none of the supplemented forms of Zn increased its concentration in blood, plasma and hair. On the other hand, the administration of Se led to an increase in the Se concentration in blood, increased the activity of GSH-Px in whole blood and the Se content in hair. Based on the proven correlation and regression relation between the Se concentration in blood and its content in hair, hair can be considered as a suitable material for the diagnosis of long-term Se status in goats. Goats with sufficient Se status are those that have more than 160 µg/kg of Se in hair dry weight.

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Improvement of Intraoperative Antibiotic Prophylaxis in Prolonged Cardiac Surgery by Automated Alerts in the Operating Room

Infection Control and Hospital Epidemiology ◽

10.1086/502109 ◽

2003 ◽

Vol 24 (1) ◽

pp. 13-16 ◽

Cited By ~ 45

Author(s):

Giorgio Zanetti ◽

Hugh L. Flanagan ◽

Lawrence H. Cohn ◽

Richard Giardina ◽

Richard Platt

Keyword(s):

Cardiac Surgery ◽

Antibiotic Prophylaxis ◽

Operating Room ◽

Spillover Effect ◽

Control Group ◽

Reminder System ◽

Preoperative Administration ◽

Group 5 ◽

The Impact

AbstractObjective:To assess the impact of an automated intraoperative alert to redose prophylactic antibiotics in prolonged cardiac operations.Design:Randomized, controlled, evaluator-blinded trial.Setting:University-affiliated hospital.Patients:Patients undergoing cardiac surgery that lasted more than 4 hours after the preoperative administration of cefazolin, unless they were receiving therapeutic antibiotics at the time of surgery.Intervention:Randomization to an audible and visual reminder on the operating room computer console at 225 minutes after the administration of preoperative antibiotics (reminder group, n = 137) or control (n = 136). After another 30 minutes, the circulating nurse was required to indicate whether a follow-up dose of antibiotics had been administered.Results:Intraoperative redosing was significantly more frequent in the reminder group (93 of 137; 68%) than in the control group (55 of 136; 40%) (adjusted odds ratio, 3.31; 95% confidence interval, 1.97 to 5.56; P < .0001). The impact of the reminder was even greater when compared with the 6 months preceding the study period (129 of 480; 27%; P < .001), suggesting some spillover effect on the control group. Redosing was formally declined for 19 of the 44 patients in the reminder group without redosing. The rate of surgical-site infection in the reminder group (5 of 137; 4%) was similar to that in the control group (8 of 136; 6%; P = .42), but significantly lower than that in the pre-study period (48 of 480; 10%; P = .02).Conclusion:The use of an automatic reminder system in the operating room improved compliance with guidelines on perioperative antibiotic prophylaxis.

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Effects of Enhancing Coach-Athlete Relationships on Youth Sport Attrition

The Sport Psychologist ◽

10.1123/tsp.6.2.111 ◽

1992 ◽

Vol 6 (2) ◽

pp. 111-127 ◽

Cited By ~ 90

Author(s):

Nancy P. Barnett ◽

Frank L. Smoll ◽

Ronald E. Smith

Keyword(s):

Dropout Rate ◽

Control Group ◽

Little League ◽

Team Success ◽

Before And After ◽

Group 5 ◽

The Impact ◽

Baseball Coaches ◽

Treatment Control Group ◽

Experimental Group

A field experiment was conducted to examine the impact of the Coach Effectiveness Training program on athlete attrition. Eight Little League Baseball coaches attended a preseason sport psychology workshop designed to facilitate desirable coach-athlete interactions. A no-treatment control group consisted of 10 coaches. Children who played for both groups of coaches were interviewed before and after the season and were contacted again the following year. At the end of the initial season, children in the experimental group evaluated their coaches, teammates, and the sport of baseball more positively than children who played for the control-group coaches. Player attrition was assessed at the beginning of the next baseball season, with control-group youngsters withdrawing at a significantly higher rate (26%) than those in the experimental group (5% dropout rate). There was no difference in mean team won-lost percentages between dropouts and returning players, which indicates that the attrition was not due to lack of team success.

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Tagging juvenile European perch (Perca fluviatilis L.) with passive integrated transponders (PIT) – impact on growth, condition, and physiological indexes

Archives of Polish Fisheries ◽

10.1515/aopf-2017-0019 ◽

2017 ◽

Vol 25 (3) ◽

pp. 201-207 ◽

Cited By ~ 2

Author(s):

Zdzisław Zakęś ◽

Maciej Rożyński ◽

Elżbieta Ziomek ◽

Krystyna Demska-Zakęś

Keyword(s):

Blood Plasma ◽

Growth Condition ◽

Negative Impact ◽

Fish Growth ◽

Control Group ◽

Mean Corpuscular Hemoglobin ◽

Feed Conversion ◽

European Perch ◽

Passive Integrated Transponders ◽

The Impact

Abstract The aim of the study was to determine the impact of intramuscular tagging with passive integrated transponders (PIT tags) on the basic condition and hematological and biochemical indexes of the blood plasma of juvenile European perch (initial body weight of approximately 80 g). Throughout the observation period, i.e., for 42 days following PIT implantation, the procedure was not noted to have had a negative impact on fish growth, condition, or feed conversion ratio. The blood plasma biochemical indicators analyzed did not differ statistically significantly between the fish tagged with PIT and the untagged control group. No significant impact from this procedure was noted in the hematological indexes tested. Only with regard to the mean corpuscular hemoglobin (MCH) parameter was the value significantly lower in the fish tagged with PIT than in the control group (P ≤ 0.05). The difference determined was 1.40 pg (28.78 vs. 30.18). The MCH level in the fish tagged with PIT most probably was within the norm for this species. High tag retention (100%) and the lack of any significant impact on condition or hematological and biochemical indexes (with the exception of MCH) permits recommending this tagging method for use in juvenile perch.

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Oral L-Arginine lower neovascularization and the number of fibroblasts but failed to increase nitric oxide and epithelialization in the healing process of male white diabetic wistar rats (Rattus norvegicus)

IJAAM (Indonesian Journal of Anti-Aging Medicine) ◽

10.36675/ijaam.v3i2.44 ◽

2019 ◽

Vol 3 (2) ◽

pp. 24

Author(s):

Fenni Liem ◽

Wimpie Pangkahila ◽

I Wayan Weta

Keyword(s):

Nitric Oxide ◽

Diabetes Mellitus ◽

Rattus Norvegicus ◽

Wistar Rats ◽

Healing Process ◽

Intervention Group ◽

Control Group ◽

White Rats ◽

Nitric Oxide Level ◽

The Mean

Introduction: Oral L-Arginine is a conditional essential amino acid that plays a role in wound healing in DM. The role of arginine in diabetic wounds is by enhancing blood circulation in the injured area and increasing oxygen supply to the wound tissue. The purpose of this study to prove the administration of oral L-Arginine toward vascularization status in wound healing of male white rats wistar diabetes mellitus. Methods: A randomized posttest only control group study using with 36 diabetic induced wistar rats (Rattus Norvegicus) aged 2-3 months and weighing 180-200gram which then divided randomly into two groups. Nitric oxide level was measured on the third day and each group was then further divided into two groups for examination of neovascularization, fibroblasts and epithelialization on the seventh day and on the tenth day. Results: Administration oral L-Arginine failed to induce any significant change in Nitric Oxide level and wound gap closure. On the other hand, the results showed that the mean neovascularization was significantly different between the two groups on the 10th day (Control group vs intervention group: 4.22±1922 vs1.89±1364; p=0.009). In addition, the mean number of fibroblast at the 10th day was also significantly different (Control group vs intervention group: 74.11±28.57 vs 38.11±20.90; p=0.008). Conclusion: In conclusion, oral L-Arginine did not significantly affect nitric oxide and epithelialization while decreased neovascularization and the number of fibroblasts on day tenth in the healing process of male white rats diabetes mellitus

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Combined effect of glyphosphate, saccharin and sodium benzoate on rats

Regulatory Mechanisms in Biosystems ◽

10.15421/021888 ◽

2018 ◽

Vol 9 (4) ◽

pp. 591-597 ◽

Cited By ~ 4

Author(s):

M. A. Lieshchova ◽

N. M. Tishkina ◽

A. A. Bohomaz ◽

P. M. Gavrilin ◽

V. V. Brygadyrenko

Keyword(s):

Body Weight ◽

Sodium Benzoate ◽

Food Additives ◽

Combined Effect ◽

Male Rats ◽

Laboratory Animals ◽

Relative Mass ◽

Control Group ◽

Expiry Date ◽

The Impact

Herbicides and food additives are included in many food products for humans. Non-used products or products beyond their expiry date are deposited in places of utilizatioin of solid municipal wastes, where they can take effects on the organisms of mouse-like rodents. Among the herbicides, glyphosphate takes first place in the world for volume of production, and is the most intensely used in agricultural farming. The discussion about negative impact on the organisms of mammals, especially against the background of using various substances and environmental factors, continues. In this study, we determined the combined effect of glyphosphate and food additives on the organism of laboratory animals, which manifested in changes in body weight, condition and indices of mass of the internal organs and blood parameters. Four groups of laboratory male rats were formed, which over 42 days received: unlimited access to clean water; 1% aqueous solution of glyphosate; 1% solution of glyphosphate and 1% solution sodium benzoate; 1% solution of glyphosphate with 1% solution of saccharin. Glyphosphate and glyphosphate with sodium benzoate and saccharin significantly reduced the daily increases in body weight of animals compared to the control group. The studied substances have notable suppressive effect on the immune system and haematopoiesis in general, which is manifested in reduce of relative mass of the thymus and spleen against the background of increase in the amount of lymphocytes in the peripheral blood. The inhibition of haematopoiesis is indicated by decrease in the amount of erythrocytes, neurophils and hemoglobin of blood of animals from the experimental groups. The impact on the digestive system of glyphosphate and food additives is indicated by occurance of the effect of “irritation” of mucous membranes, and, as a result, disorders in absorption followed by the disorder in metabolic processes. A dysbalance occurs in enzymic systems of the organism, which is manifested in distrophic processes, especially in the liver parenchyma, indicated by the activity of blood enzymes (ALT, AST, alkaline phosphatase), total number and ratio of proteins of blood plasma. We determined the impact of glyphosphate and its mixes with benzoate and saccharin on the pancreas, which manifests in severe pancreatitis with steep increase in the level of glucose of blood. The results of the study allow us to state that mixture of glyphosphate and food additives can cause toxic effect in animals and humans, which often contact with herbicides.

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Diastolic disorder inherent to doxorubicin cardiotoxicity

The Moldovan Medical Journal ◽

10.52418/moldovan-med-j.64-4.21.04 ◽

2021 ◽

Vol 64 (4) ◽

pp. 23-28

Author(s):

Lilia Tacu ◽

◽

Valeriu Cobet ◽

Keyword(s):

Diastolic Pressure ◽

Isolated Heart ◽

Physiological Solution ◽

Control Group ◽

White Rats ◽

Failure Evolution ◽

Diastolic Stiffness ◽

Relationship Of ◽

The Impact

Background: The doxorubicin (Dx) cardiotoxicity is manifested by a marked heart failure evolution. The impact of Dx on lusitrop functions of the heart and the inherent diastolic disorders have a theoretical and practical value for the connection cardiology-oncology. Material and methods: Dx cardiotoxicity was reproduced by its administration i/p in white rats in cumulative dose 16 mg/kg (Dx group n=9). Control group (n=9) received only physiological solution. The study was performed in vitro by using models of isolated heart perfusion in either isovolumic or exterior working regimens. The assayed indices of diastole functioning were: left ventricle (LV) end-diastolic pressure (LVEDP), diastolic stiffness, isovolumic relaxation velocity (-dP/dTmax) and protodiastolic pressure of LV (LVPDP). Results: The indices of diastolic disorders induced by Dx were elevation of LVEDP, diastolic stiffness and LVPDP in a range of 97-168% comparing to control as well as diminution of -dP/dTmax in the physiological pattern of hemodynamics. LVEDP increased more in conditions of calcium overloading or endothelin-1 (ET-1) action that are involved in pathogenesis of diastolic rigidity. Dx action led to decrease of myocardium resistance to ischemiareperfusion action resulting in the LVEDP elevation by 53% comparing to control. Conclusions: 1. Diastolic disorders inherent to Dx cardiotoxicity are manifested by the increase of LVEDP and diastolic stiffness. 2. Diastolic disorders compromised the volume-pressure relationship of LV, the adaptation of the heart to effort with volume, being more pronounced during the action of calcium excess and ET-1

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