BIOLOGICALLY ACTIVE COMPOUNDS CONTENE ASSESSMENT IN CINNAMON ROSE (ROSA MAJALIS HERRM.) FRUITS FROM THE KUZBASS FLORA

Author(s):  
Egorova I.N. ◽  
Maltseva E.M. ◽  
Bolshakov V.V.

Cinnamon rose (Rosa majalis Herrm.) is the one of two species of rose hips growing in the Kemerovo region. The rose hips pharmacopoeial raw material are fruits which used as a vitamin agent. The rose hips pharmacological effect is due to the rich complex of biologically active substances contained in its fruits. Cinnamon rose belongs to the high-vitamin species. Ascorbic acid content can reach 4-14% in it. Due to the high content of organic acids and polyphenols, rose hips and preparations based on it have a fairly strong antioxidant effect. They are often used to prevent vitamin C deficiency, which is especially important for the region local population living in a territory with a harsh continental climate, characterized by long cold winters. Therefore, the rose hips from Kuzbass flora further study is an urgent goal to expand the medicinal plant raw material base and to provide the population of the region with a vitamin product. The study was aimed at assessing some biologically active compounds content (tannins, organic acids, ascorbic acid) in the cinnamon rose fruits growing in the region. Ascorbic acid quantitative determination was carried out by HPLC, organic acids and tannins - by titration. The analysis showed that the ascorbic acid content in the raw materials collected in the Topkinsky district of the region reaches 0,450±0,003 %, organic acids - 3,728±0,051 %, which corresponds to the PI requirements for this type of raw material. Tannins quantitative content reaches 5.76 %. Obtained experimental data comparative analysis with the literature confirmed the fact that one of the main factors affecting the rose hips vitamin content is the geographical factor.

2021 ◽  
Vol 17 (3) ◽  
pp. 210-217
Author(s):  
N. I. Dzhurenko ◽  
O. P. Palamarchuk ◽  
T. O. Shcherbakova ◽  
V. F. Gorobets ◽  
V. І. Todorova

Purpose. To reveal the raw material potential of the representatives of the genus Paeonia L. introduced in the M. M. Gryshko National Botanical Garden of the National Academy of Sciences of Ukraine (NBG) in terms of the content of biologically active compounds. Methods. Introduced plants of the genus Paeonia: P. lactiflora Pall., P. officinalis L. and its hybrid forms: P. officinalis ‘Rubra Plena’, F1 (P. officinalis ‘Rub­ra Plena’ ´ P. peregrina Mill.), cultivar NBG ‘Kvazimodo’ (P. lactiflora ‘M-lle Jeanne Riviere’ ´ F1 [P. officinalis ‘Rubra Plena’ ´ P. peregrina)], P. anomala L. were the object of the research. The content of polyphenolic compounds, homopolysaccharides, ascorbic acid and other substances was determined by spectrophotometry, titrimetry and colorimetry. The analysis of biologically active compounds was carried out by the method of high performance liquid chromatography (HPLC). Results. Species and varietal differences in the amount of polyphenols in plants of various species and forms were determined. It was found that the roots of the studied F1 plants (P. officinalis ‘Rubra Plena’ ´ P. peregrina), cultivar NBG ‘Kvazimodo’, P. lactiflora contain a much higher amount of polysaccharides compared to the roots of P. anomala. In peony plants, the average share of vitamin C is 37.65% and varies among representatives of the genus in the range from 22.10 ± 2.18 [F1 (P. officinalis ‘Rubra Plena’ ´ P. peregrina)] to 47.60 ± 3.69 (P. anomala) and 49.30 ± 5.50% (P. lactiflora). The maximum content of carotenoids was found in the roots of P. officinalis ‘Rubra Plena’ (2.10 ± 0.21 mg%) and F1 (P. officinalis ‘Rubra Plena’ ´ P. peregrina) (2.20 ± 0.13 mg%). A significant amount of free gallic acid was observed in the roots of P. anomala, and halotannins in P. lactiflora. Conclusions. The results of phytochemical studies of plants of species, hybrids and cultivars of the genus Paeonia introduced in the  M. Gryshko National Botanical Garden of the NAS of Ukraine showed that they accumulate a significant level of basic biologically active compounds: flavonoids, tannins, mono- and polysaccharides, pigments roots of P. lactiflora and P. officinalis are promising in terms of the complex of active ingredients, since they contain them in greater quantities than those of P. anomala. Hybrid forms of P. officinalis ‘Rubra Plena’, F1 (P. officinalis ‘Rubra Plena’ ´ P. peregrina), cultivar ‘Kvazimodo’, created on the basis of these species, are not inferior to the parent species in terms of the content of phenolic compounds, sugars, pigments and ascorbic acid. In the roots, the share of peoniflorin is for P. lactiflora (1.95%),  anomala (1.09%), P. officinalis ‘Rubra Plena’ (0.95%), P. officinalis (0.78%). The studied species, hybrids and cultivars are offered for cultivation in the purpose of harvesting medicinal raw materials as a source of biologically active compounds with the possibility of supplementing or even repla­cing the official species of anomalous peony (P. anomala).


Author(s):  
Dalija Segliņa ◽  
Inta Krasnova ◽  
Sanita Alsiņa

Abstract Lonicera caerulea L., also known as honeysuckle berries, are rich in biologically active compounds, which makes them a valuable raw material for the development of functional foods. The objects of the research were three honeysuckle cultivars (‘Zoluška’, ‘Siņaja Ptica’, ‘Goluboje Vereteno’), four genotypes (No 2A; No 3; No 5; No 8) and kefir, enriched with various amounts (3–10%) of fruit puree. Physical and chemical analyses to determine the qualitative indicators of raw materials and products were performed. The degree of liking of kefir was assessed using sensory analysis: hedonic and line scales. The results obtained showed that among the cultivars the highest content of anthocyanins (1103.5 mg·100 g−1), total phenols (693.3 mg·100 g−1), flavonoids (753.9 mg·100 g−1), and tannins (1.6 mg·100 g−1), were found in ‘Siņaja Ptica’ berries, while among the genotypes, berries of No 8 had the highest concentration of the aforesaid compounds. Both samples also showed the highest antiradical activity: ABTS+• on average by 71.7% and DPPH− by 43.7%. According to the sensory evaluation, kefir with 10% honeysuckle puree was rated the highest. Honeysuckle berries could be used as a promising source of natural anti-oxidants in future research aimed at developing different new products that could meet consumer expectations.


Author(s):  
E. F. Semyonova ◽  
I. M. Teplitskaya ◽  
M. A. Goncharov ◽  
D. A. Goncharov

Based on the content analysis of the sources of patent and scientific literature on the biotechnology of lichens as a medicinal raw material, systematized modern information is provided, as well as original experimental data regarding the cultivation of botanical species of lichens of various ecological and geographical groups under controlled conditions. Since ancient times, lichens havebeen used as medicines and are included in the pharmacopoeias of various countries. At present, methodological approaches have been developed for the cultivation of lichens (genera Usnea, Rhinocarpon, Umbilicara ) and their components: phycobiont (genera Nostoc, Chlorella ), mycobiont ( Petrusaria pertiza, Leydea parasema, Alternaria sp.). The main biologically active compounds (usnic acid, lichenin, cetrarin, water-soluble vitamins, calcium oxalate) and the pharmacological effects caused by them (antimicrobial, stimulating appetite, enveloping, anti-inflammatory, anti-burn, regenerating). Biotechnological approaches can be implemented for species cloning, reproduction, creation of a bank of cell cultures of lichens and their components in order to preserve economically valuable producers in an active state and develop technologies for obtaining biomass and pharmacologically valuable biologically active compounds.


2020 ◽  
Vol 20 (5-6) ◽  
pp. 164-169
Author(s):  
Evgeny E. Кurdyukov ◽  
Elena F. Semenova ◽  
Aleksandr V. Mitishev ◽  
Yаkov P. Moiseev ◽  
Anna V. Kuznetsova

Relevance. Standardization of plant raw material derived from stevia is a topical issue, since this plant (Stevia rebaudiana Bertoni) contains sweet diterpene glycosides and is used as a natural sugar substitute. In addition, stevia contains flavonoids which are biologically active compounds that exert antioxidant, anti-inflammatory and capillary-strengthening properties. Currently, spectrophotometric methods are used to identify and quantify flavonoids in medicinal plants. They are fast, convenient and do not require sophisticated equipment. The aim of our paper is comparative phytochemical study of the content of the total flavonoids in stevia raw material of different origin. Materials and methods. The study material was dried stevia leaves (Stevia rebaudiana Bertoni), grown in the Penza Region, the Republic of Crimea, the Krasnodar territory, and raw materials imported from Paraguay and India. The content of the total flavonoids was estimated by differential spectrophotometry calculated with reference to cynaroside. Results. The study of the quantity of flavonoids in the raw material of stevia of different origin was carried out. In the presence of aluminum chloride stevia flavonoids form a complex compound at the absorption maximum of 408 2 nm. The content of flavonoids in different species of stevia are found to range from 1.13% to 1.74%. Conclusion. The obtained results allow to recommend stevia leaves as a source of flavonoids along with other available medicinal plants. It is advisable to classify the complex of flavonoids as the second group of biologically active compounds of stevia leaves.


2019 ◽  
Vol 7 (1) ◽  
pp. 25-33
Author(s):  
Olena Yerenko ◽  
Galina Smoylovska ◽  
Taya Khortetska

The level of studying of Inula L. genus species, growing on the territory of Ukraine, is insufficient today. The study of chemical composition, accumulation of biologically active substances, pharmacological action of investigated grass species’ extracts of Inula L. genus of Ukrainian flora are of current interest. The aim of the work is making qualitative and comparative analysis of ascorbic, organic acids and tannic substances content in the raw material of investigated species of Inula L. genus (Inula britannica L. and Inula helenium L.). Quantitative determination was carried out by volume titration method. The presence of ascorbic acid, organic acids and tannic substances in the raw material of Inula L. genus investigated species was established with the help of paper chromatography and thin-layer chromatography methods. The quantitative determination of total organic acids was carried out according to XI edition of State Pharmacopoeia methods in terms of malic acid. Water extracts were studied for the tannins presence in raw material of Inula L. genus investigated species. Compounds identification was carried out through chemical reactions in accordance with Ukrainian State Pharmacopeia. The results obtained showed the perspective of further study of the biological activity of Inula britannica L. and Inula helenium L.


2021 ◽  
pp. 62-66
Author(s):  
А. Туреханова ◽  
Қ. Тоштай ◽  
М. Дуйсебаева ◽  
Т. Тұрсонжан ◽  
Ж. Жеңіс

Согласно литературным данным, подсолнечное масло богато полезными веществами, однако, в зависимости от степени очистки масла, количество этих компонентов варьируется. В данном исследовании был изучен дистиллят жирных кислот подсолнечного масла. Согласно полученным данным, более 40% состава кислот приходится на линолевую кислоту с F-витаминной активностью. В большом количестве содержатся стерины, составляющие 44% неомыляемого остатка. Токоферолы составляют 9% неомыляемого остатка, 93% из них приходится на a-токоферол. Helianthus annuus L необходим для приготовления натурального мыла, сочетает в себе способ приготовления медицинского мыла с технологией производства. Экстракт из стебля растения. Это свежее натуральное мыло содержит много полезных микро-и макроэлементов, биологически активные соединения и макро - и микроэлементы растения Helianthus annuus L были определены атомно-эмиссионным методом. Основными из них были K (44,9 мг/г), (377,745 мг/г), Na (3,91 мг/г), (7,56 мг/г) и Mg (3,06 мг/г), (1,495 мг/г). Эти элементы полезны для нашего организма и обладают целебными свойствами. Кроме того, в составе растения H. Annuus L. были определены биологически активные вещества, такие как флавоноиды, алкалоиды, органические кислоты, кумарины и витамины. Эти выявленные данные позволяют получать мыло с высоким качеством, а также целебными свойствами. Helianthus annuus L salt, which is necessary for the preparation of natural soap, combines the method of preparation of medical soap production technology. The extract from the stem of the plant. This fresh natural soap contains many useful micro and macronutrients, biologically active compounds and macro - and microelements of the plant Helianthus annuus L were determined by atomicemission method. Main of them were K (44.9 mg/g), (377.745 mg/g), Na (3.91 mg/g), (7.56 mg/g) and Mg (3.06 mg/g), (1.495 mg/g). These elements are useful for our body and have healing properties. In addition, biologically active substances such as fiavonoids, alkaloids, organic acids, coumarins, and vitamins were found in the composition of the plant H. annuus L. These identied data allow us to obtain soap with a high composition and quality and healing properties


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