Aktivitas ekstrak etanol daun singawalang (Petiveria alliacea L.)  dan fraksinya sebagai antidiabetes

Abstrak Diabetes melitus (DM) adalah sekelompok gangguan metabolik yang ditandai dengan hiperglikemia. Salah satu tanaman yang mempunyai efek antidiabetes adalah daun singawalang (Petiveria alliacea L.). Penelitian ini bertujuan untuk menguji aktivitas antidiabetes dengan model hewan defisiensi insulin dan penghambatan enzim alfa glukosidase. Pengujian defisiensi insulin dilakukan menggunakan mencit induksi aloksan. Mencit dikelompokkan menjadi 11 kelompok, yaitu normal, kontrol negatif, kontrol positif (glibenklamid 0,65 mg/kgbb), ekstrak etanol (dosis 80 dan 160 mg/kgbb), fraksi n-heksana (dosis 80 dan 160 mg/kgbb), fraksi etil asetat (dosis 80 dan 160 mg/kgbb), dan fraksi air (dosis 80 dan 160 mg/kgbb). Pemberian bahan uji dilakukan berulang setiap hari selama 14 hari dan kadar glukosa darah diukur pada hari ke-7, 14, 17, dan 19. Kemudian hewan dikorbankan, dilakukan isolasi pankreas, dan dihitung luas pulau Langerhans, jumlah sel alfa dan beta pankreas. Pada uji hambat enzim alfa glukosidase, dilakukan penentuan nilai IC50 tiap fraksi terhadap aktivitas enzim, dan akarbose digunakan sebagai pembanding. Hasil uji defisiensi insulin menunjukkan bahwa ekstrak etanol dosis 80 dan 160 mg/kgbb memiliki kemampuan untuk menurunkan kadar glukosa darah. Berdasarkan hasil histologi pankreas juga menunjukkan bahwa ekstrak etanol dosis 80 dan 160 mg/kgbb mengurangi jumlah sel alfa pankreas, diperkirakan dapat menurunkan sekresi glukagon. Pada metode penghambatan enzim alfa glukosidase fraksi n-heksana dan fraksi air menunjukkan adanya penghambatan aktivitas enzim alfa glukosidase yang lebih baik dibandingkan akarbose. Kesimpulan dari penelitian ini adalah ektrak etanol daun singawalang dan fraksinya mempunyai aktivitas sebagai antidiabetes. Kata kunci: Daun singawalang, defisiensi insulin, enzim alfa glukosidase. In vivo and in vitro activity of ethanol extracts from the leaves of singawalang (Petiveria alliacea l.) and its fractions as antidiabetic Abstract Diabetes mellitus (DM) is a group of metabolic disorders characterized by hyperglycemia. One of the plants that has antidiabetic effect is the leaves of singawalang (Petitiia alliacea L.). This study aims to examine antidiabetic activity with animal model of insulin deficiency and inhibition of alpha glucosidase enzyme. Tests of insulin deficiency were performed using alloxan induction mice. The mice were grouped into 11 groups, normal, negative control, positive control (glibenclamide 0.65 mg/kgbw), ethanol extract (doses 80 and 160 mg/kgbw), n-hexane fractions (doses 80 and 160 mg/kgbw), ethyl acetate doses 80 and 160 mg/kgbw), and water fractions (doses of 80 and 160 mg/kgbw). Testing was performed daily for 14 days and blood glucose was measured on days 7, 14, 17, and 19. Later animals were sacrificed, isolated pancreas, and calculated the area of Langerhans Island, the number of alpha and beta cells of the pancreas. In the alpha glucosidase enzyme inhibition test, IC50 values were determined for each fraction of enzyme activity, and the acarbose was used as a comparison. Insulin deficiency test results showed that ethanol extract dose 80 and 160 mg/kg bw has the ability to lower blood glucose levels. Based on histological results of the pancreas also showed that ethanol extract dose 80 and 160 mg/kgbw reduce the number of pancreatic alpha cells, is expected to decrease glucagon secretion. In the inhibition method of alpha glucosidase enzyme n-hexane fraction and water fraction showed the inhibition of alpha glucosidase enzyme activity better than akarbose. The conclusion of this research is the ethanol extract of singawalang leaves and fraction has activity as antidiabetes. Keywords: Diabetes mellitus, leaf singawalang, insulin deficiency, the enzyme alpha-glucosidase.

Download Full-text

Aktivitas ekstrak etanol daun singawalang (Petiveria alliacea L.) dan fraksinya sebagai antidiabetes

Kartika Jurnal Ilmiah Farmasi ◽

10.26874/kjif.v5i2.113 ◽

2017 ◽

Vol 5 (2) ◽

pp. 68

Author(s):

Elis Susilawati ◽

I Ketut Adnyana ◽

Neng Fisheri

Keyword(s):

Diabetes Mellitus ◽

Enzyme Activity ◽

Blood Glucose ◽

Ethanol Extract ◽

Antidiabetic Activity ◽

Insulin Deficiency ◽

Water Fraction ◽

Petiveria Alliacea ◽

Alpha Glucosidase

Abstrak Diabetes melitus (DM) adalah sekelompok gangguan metabolik yang ditandai dengan hiperglikemia. Salah satu tanaman yang mempunyai efek antidiabetes adalah daun singawalang (Petiveria alliacea L.). Penelitian ini bertujuan untuk menguji aktivitas antidiabetes dengan model hewan defisiensi insulin dan penghambatan enzim alfa glukosidase. Pengujian defisiensi insulin dilakukan menggunakan mencit induksi aloksan. Mencit dikelompokkan menjadi 11 kelompok, yaitu normal, kontrol negatif, kontrol positif (glibenklamid 0,65 mg/kgbb), ekstrak etanol (dosis 80 dan 160 mg/kgbb), fraksi n-heksana (dosis 80 dan 160 mg/kgbb), fraksi etil asetat (dosis 80 dan 160 mg/kgbb), dan fraksi air (dosis 80 dan 160 mg/kgbb). Pemberian bahan uji dilakukan berulang setiap hari selama 14 hari dan kadar glukosa darah diukur pada hari ke-7, 14, 17, dan 19. Kemudian hewan dikorbankan, dilakukan isolasi pankreas, dan dihitung luas pulau Langerhans, jumlah sel alfa dan beta pankreas. Pada uji hambat enzim alfa glukosidase, dilakukan penentuan nilai IC50 tiap fraksi terhadap aktivitas enzim, dan akarbose digunakan sebagai pembanding. Hasil uji defisiensi insulin menunjukkan bahwa ekstrak etanol dosis 80 dan 160 mg/kgbb memiliki kemampuan untuk menurunkan kadar glukosa darah. Berdasarkan hasil histologi pankreas juga menunjukkan bahwa ekstrak etanol dosis 80 dan 160 mg/kgbb mengurangi jumlah sel alfa pankreas, diperkirakan dapat menurunkan sekresi glukagon. Pada metode penghambatan enzim alfa glukosidase fraksi n-heksana dan fraksi air menunjukkan adanya penghambatan aktivitas enzim alfa glukosidase yang lebih baik dibandingkan akarbose. Kesimpulan dari penelitian ini adalah ektrak etanol daun singawalang dan fraksinya mempunyai aktivitas sebagai antidiabetes. Kata kunci: Daun singawalang, defisiensi insulin, enzim alfa glukosidase. In vivo and in vitro activity of ethanol extracts from the leaves of singawalang (Petiveria alliacea l.) and its fractions as antidiabetic Abstract Diabetes mellitus (DM) is a group of metabolic disorders characterized by hyperglycemia. One of the plants that has antidiabetic effect is the leaves of singawalang (Petitiia alliacea L.). This study aims to examine antidiabetic activity with animal model of insulin deficiency and inhibition of alpha glucosidase enzyme. Tests of insulin deficiency were performed using alloxan induction mice. The mice were grouped into 11 groups, normal, negative control, positive control (glibenclamide 0.65 mg/kgbw), ethanol extract (doses 80 and 160 mg/kgbw), n-hexane fractions (doses 80 and 160 mg/kgbw), ethyl acetate doses 80 and 160 mg/kgbw), and water fractions (doses of 80 and 160 mg/kgbw). Testing was performed daily for 14 days and blood glucose was measured on days 7, 14, 17, and 19. Later animals were sacrificed, isolated pancreas, and calculated the area of Langerhans Island, the number of alpha and beta cells of the pancreas. In the alpha glucosidase enzyme inhibition test, IC50 values were determined for each fraction of enzyme activity, and the acarbose was used as a comparison. Insulin deficiency test results showed that ethanol extract dose 80 and 160 mg/kg bw has the ability to lower blood glucose levels. Based on histological results of the pancreas also showed that ethanol extract dose 80 and 160 mg/kgbw reduce the number of pancreatic alpha cells, is expected to decrease glucagon secretion. In the inhibition method of alpha glucosidase enzyme n-hexane fraction and water fraction showed the inhibition of alpha glucosidase enzyme activity better than akarbose. The conclusion of this research is the ethanol extract of singawalang leaves and fraction has activity as antidiabetes. Keywords: Diabetes mellitus, leaf singawalang, insulin deficiency, the enzyme alpha-glucosidase.

Download Full-text

BIOASSAY-GUIDED EVALUATION OF FICUS SEMICORDATA FOR ANTIDIABETIC ACTIVITY

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i3.16441 ◽

2017 ◽

Vol 9 (3) ◽

pp. 71 ◽

Cited By ~ 1

Author(s):

Virender Kaur ◽

Kumud Upadhyaya ◽

Milind Pande

Keyword(s):

Diabetes Mellitus ◽

Early Stage ◽

Ethanol Extract ◽

Postprandial Hyperglycemia ◽

Alpha Amylase ◽

Antidiabetic Activity ◽

Biological Studies

Objective: The early stage of diabetes mellitus type 2 is associated with postprandial hyperglycemia. The therapeutic approach involved in the treatment of type 2 diabetes mellitus is the use of agents that can decrease postprandial hyperglycemia by inhibiting carbohydrate digesting enzymes. In an effort of identifying herbal drugs which may become useful in the prevention or mitigation of diabetes, the antidiabetic activity of Ficus semicordata (FS) and its constituents were studied. The present study was undertaken in part to identify the potent antihyperglycemic fraction from the ethanol extract of the plant, using bioassay guided evaluation.Methods: The ethanol extract of Ficus semicordata were fractionated to obtain chloroform, ethyl acetate, n-butanol and ethanol extracts which were tested for alpha-amylase, alpha-glucosidase inhibitory, properties. Further fractionation of the more active ethanol fraction yielded isolates FS-1 and FS-2 which were tested for in vivo antidiabetic activity using Streptozotocin (STZ)-induced diabetic rats.Results: Ethanol extract from leaves of the plant showed notable alpha-amylase (IC50 = 3.352µg/ml and alpha-glycosidase inhibitory activity (IC50= 3.448µg/ml) as compared to standard acarbose (IC50 = 3.175µg/ml. Subfraction FS-1 and FS-2 which were tested for in vivo antidiabetic activity using acute STZ-induced diabetic rats significantly (*p<0.05, **p<0.01, *** p<0.001) reduced blood glucose level.Conclusion: The Ficus semicordata plant extracts and the fractionated components could be used as a natural antidiabetic after comprehensive in vitro and in vivo biological studies.

Download Full-text

Isolation of Chemical Constituents and Invitro Antidiabetic Activity of Mirabilis Jalapa Flower Extracts

Journal of Pharmaceutical Research International ◽

10.9734/jpri/2021/v33i60a34501 ◽

2021 ◽

pp. 412-419

Author(s):

Deepth D. Kodical ◽

Jennifer Fernandes ◽

Jane Mathew ◽

K. Deepthi ◽

K. Vijay Bhaskar

Keyword(s):

Chemical Constituents ◽

Ethanol Extract ◽

Antidiabetic Activity ◽

Inhibition Assay ◽

Phytochemical Investigation ◽

In Vitro Investigation ◽

Mirabilis Jalapa ◽

Phytochemical Constituents ◽

Alpha Glucosidase

Objectives: The present work was designed to investigate the phytochemical and in vitro screening of the flower extracts of Mirabilis jalapa. Materials and Methods: Phytochemical constituents were isolated using column chromatography and characterized of the compounds were carried out using IR, 1H NMR, 13C NMR and mass spectroscopy data. In vitro anti-diabetic activity was evaluated by alpha-amylase inhibition assay and Alpha-glucosidase inhibition assay. Results: Phytochemical investigation of the ethanol extract revealed the presence of a flavonoid Kaempferol and a triterpenoid Lupeol. In vitro investigation indicated that the Mirabilis jalapa flowers extracts has considerable anti-diabetic activity Conclusion: The anti-diabetic activity may due to the presence of phytoconstituents like flavonoids, triterpenoids, steroids.

Download Full-text

Examination of the polyphenol content and bioactivities of Prunus spinosa L. fruit extracts

Archives of Biological Sciences ◽

10.2298/abs191217004v ◽

2020 ◽

Vol 72 (1) ◽

pp. 105-115

Author(s):

Ivona Velickovic ◽

Zeljko Zizak ◽

Nemanja Rajcevic ◽

Marija Ivanov ◽

Marina Sokovic ◽

...

Keyword(s):

Diabetes Mellitus ◽

Ethanol Extract ◽

Diabetes Mellitus Type ◽

Antidiabetic Activity ◽

Total Phenolic ◽

Aqueous Extracts ◽

Type Ii ◽

Diabetes Mellitus Type Ii ◽

Prunus Spinosa

We investigated the total phenolic and flavonoid contents and the anthocyanin profiles in aqueous, ethanol and acetone extracts of Prunus spinosa (Rosaceae) fruit, and their antioxidant, antibacterial, antifungal, antidiabetic and antitumor properties. The contribution of polyphenol contents to the bioactivity of the extracts was calculated and observed through Pearson?s coefficient of correlation. The acetone extract was the richest in phenols and anthocyanins and the ethanol extract in flavonoids. Cyanidin was the most abundant anthocyanin compound in all examined extracts. The ethanol extract showed the most promising antioxidant activity in DPPH, ABTS and FRAP assays. Tested bacteria were more affected by the ethanol than by the aqueous extract. Both the ethanol and aqueous extracts exhibited potential antidiabetic effects, observed as inhibition of ?-amylase and ?-glucosidase, enzymes linked with diabetes mellitus type II. The ethanol extract was a potent ?-glucosidase-inhibitor with a significantly lower IC50 value than the positive control, glucobay, used to treat diabetes mellitus type II. Neither the ethanol nor the aqueous extracts had any effects on tested human malignant cell lines. Our results indicate that the ethanol extract showed the most pronounced in vitro antioxidant and antimicrobial effects, and a potential antidiabetic activity, which can be ascribed to its high flavonoid content. Our results indicate that research of compounds, particularly of flavonoids present in the ethanol extract and their anti-diabetic properties should be examined further.

Download Full-text

Acute toxicity and antidiabetic activity of Asystacia gangetica leaf ethanol extract

Nutrition & Food Science ◽

10.1108/nfs-11-2018-0329 ◽

2019 ◽

Vol 50 (1) ◽

pp. 179-196 ◽

Cited By ~ 1

Author(s):

Eunice Ngozi Ezembu ◽

Chioke Amaefuna Okolo ◽

James Obiegbuna ◽

Florence Chika Ikeogu

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Acute Toxicity ◽

Glucose Level ◽

Ethanol Extract ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Content Type ◽

Acute Toxicity Study ◽

Different Doses

Purpose The purpose of this study is to examine the acute toxicity and antidiabetic activity of Asystacia gangetica leaf ethanol extract. Design/methodology/approach The study was designed as completely randomized in vivo experimental model. Where acute toxicity study was carried out using 30 albino mice, randomly assigned into six groups of five mice each. Toxicity signs and mortality were observed in the rats within a period of 24 h. The acute and sub-acute antidiabetic study was carried out using 50 rats, randomly assigned into five groups of 10 rats each. The rats’ blood glucose levels were determined and used to assess the acute and sub-acute antidiabetic activity of the extract. Findings Results obtained from the acute toxicity study indicated no death in any of the study groups, even at 5,000 mg/kg body weight and showed no signs of toxicity. The acute antidiabetic study showed that treatment with 400 mg/kg of the extract significantly (p = 0.01) lowered glucose level in the diabetic rats from 430.6 to 177.4 mg/dl while 800 mg/kg brought down glucose level from 370 to 144.2 mg/dl by the end of 6 h following administration when compared with the diabetic control group. It was observed that the effect of the extract mostly at 800 mg/kg also compared favorably with that of the standard drug (glibenclamide), which lowered glucose level in diabetic rats from 374.2 to 176.4 mg/dl. Furthermore, the significant reduction was evident from 4, 2 and 2 h for 400 mg/kg extract, 800 mg/kg extract and 5 mg/kg glibenclamide, respectively. At sub-acute level the blood glucose was lowered from 155.6 to 127.2 mg/dl, 137 to 124.4 mg/dl and 151.8 to 121.8 mg/dl for diabetic rats treated with 400 mg/kg, 800 mg/kg and 5 mg/kg glibenclamide, respectively, when compared to the diabetic untreated rats, which ranged from 417.6 to 358.6 mg/dl. The biochemical profile, lipid profile and hematological examination were all positively restored near to normal with the herbal treatment at the different doses. At histopathology level, the liver of the rats treated with 400 mg/kg had moderate portal inflammation without interface or lobular hepatitis while that of 800 mg/kg showed severe portal inflammation with the interface and lobular hepatitis with extensive confluents necrosis. The pancreatic cells of the treated rat showed no significant difference in the β-cells of the islets of Langerhans with hyperplasia of the acinar cell when compared to the diabetic untreated. Research limitations/implications The record of no death and signs of toxicity implies that the extract is safe for consumption even at a high dosage of 5,000 mg/kg body weight. The significant (p = 0.01) reduction in the plasma glucose level of the treated rats as compared to the control is an indication of blood glucose-lowering potential of the extract at two different doses. The significant reduction evident of the extract at different hours and days for the two doses implies that the extract rate of lowering potentials is dose-dependent. The evidence of moderate-severe portal inflammation with the interface and lobular hepatitis at 800 mg/kg treatment is an indication that the intake of this herb at high dosage for long period is not safe for the liver tissue. Practical implications The outcome of this study suggested that the Asystacia gangetica should also be used as a vegetable in-home food preparation and food processing to use its antidiabetic effect. The herbal extract could also be incorporated into a food product and processed into herbal tea bag for convenient. The subjection of this herbal plant to heat treatment during processing could be a possible avenue to make it safe. Social implications The economic burden and complications of diabetes mellitus management will be reduced if the practical implication of this research finding is implemented in foods as vegetable and in functional food production. Originality/value This study revealed that Asystacia gangetica leaf extract may be safe and effective for use at a low dose for acute management of diabetes mellitus. This research may be of value to those living with diabetes mellitus.

Download Full-text

Hypoglicemic and Antioxidant Activity of Petiveria alliacea in Diabetic Rat Models

Biomolecular and Health Science Journal ◽

10.20473/bhsj.v3i1.19129 ◽

2020 ◽

Vol 3 (1) ◽

pp. 19

Author(s):

Vania Azalia Gunawan ◽

Harlina Soetjipto ◽

Arifa Mustika

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

Ethanolic Extract ◽

The Body ◽

Antidiabetic Activity ◽

Diabetic Rat ◽

High Dose ◽

Leaf Extracts ◽

Chronic Hyperglycemia ◽

Petiveria Alliacea

Introduction: Diabetes mellitus is a degenerative disease characterized by chronic hyperglycemia conditions in the body. Various complications of diabetes mellitus are caused by oxidative stress condition. Petiveria alliacea (P. alliacea) is a potential plant and easy to grow in hot regions. Leaf extracts of P. alliacea contain flavonoids and tannins which work as antidiabetic and antioxidant. In addition, other compounds found in P. alliacea leaf extracts like linoleic acid and allantoin show an increase in insulin secretion. Therefore, this study aimed to determine the antidiabetic activity of ethanolic extract of P. alliacea. Methods: We investigated the hypoglycemic and antioxidant effect of P. alliacea on STZ-induced diabetic rats. Rats were randomly divided into six groups named normal control, diabetes control, metformin (150 mg/kg/d), low dose of P. alliacea (90 mg/kg/d), intermediate dose (180 mg/kg/d), and high dose (360 mg/kg/d). Rats were orally given the treatment daily in the morning for fourteen days. At the end of the study, blood glucose level was measured and rats were sacrificed to measure blood malondialdehyde level.malondialdehydeResults: P. alliacea extract dose of 90 mg/kg and 360 mg/kg, and also metformin significantly decrease blood glucose levels. P. alliacea extract dose of 360 mg/kg was able to lower blood malondialdehyde level significantly which were not obtained on metformin. Conclusion: This finding suggests that ethanolic extract of P. alliacea possess antidiabetic effect at least on rats.

Download Full-text

Effect of Bay Leaf Ethanol Extract on Blood Glucose Level in Patients with Type 2 Diabetes Mellitus

Strengthening Hospital Competitiveness to Improve Patient Satisfaction and Better Health Outcomes ◽

10.26911/the6thicph-fp.05.07 ◽

2019 ◽

Author(s):

Tri Widyawati ◽

◽

M. Aron Pase ◽

Milahayati Daulay ◽

Imam Bagus Sumantri ◽

...

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Ethanol Extract

Download Full-text

Phytochemical evaluation and in vitro antidiabetic efficiency of isopropanolic leaf extract of pimenta racemosa

Kongunadu Research Journal ◽

10.26524/krj.2020.21 ◽

2020 ◽

Vol 7 (2) ◽

pp. 50-55

Author(s):

Anitha T A ◽

Pakutharivu T ◽

Nirubama K ◽

Akshaya V

Keyword(s):

Diabetes Mellitus ◽

Glucose Uptake ◽

Inhibitory Activity ◽

Leaf Extract ◽

Yeast Cells ◽

Antidiabetic Activity ◽

Leaf Extracts ◽

Pimenta Racemosa

The traditional herbal medicines are mainly obtained from plants are used in the management of Diabetes mellitus. The main objective of this work was to assess the presence of phytochemical compounds and to evaluate the in vitro antidiabetic activity of isopropanolic extracts of Pimenta racemosa leaves by studying their α-amylase inhibitory activity and glucose transport across yeast cells. Screening of phytochemicals showed positive results for alkaloids, steroids, cardiac glycosides, terpenoids, reducing sugars, anthraquinones, and results of in vitro α-amylase inhibitory studies demonstrated there was a dose-dependent increase in percentage inhibitory activity by the isopropanolic leaf extracts of Pimenta racemosa. At a concentration of 1 mg/ml, the extract showed a percentage inhibition 33.6 and for 5 mg/ml it was 91.2. The glucose uptake study was also studied through yeast cells by analyzing theamount of glucose remaining in the medium after a specific time intervals. It serves as an indicator for the capability of isopropanolic leaf extracts of Pimenta racemosa to transport the glucose into yeast cells. As a result, we found that the isopropanolic leaf extract of Pimenta racemosa have inhibitory activity against αamylase and also, which is efficient in glucose uptake. This therapeutic potentiality of Pimenta racemosa could be exploited in the treatment of Type 2 Diabetes mellitus. Further studies are also required to elucidate whether the plant have antidiabetic potential by in vivo for corroborating the traditional claim of the plant.

Download Full-text

Oral DhHP-6 for the Treatment of Type 2 Diabetes Mellitus

International Journal of Molecular Sciences ◽

10.3390/ijms20061517 ◽

2019 ◽

Vol 20 (6) ◽

pp. 1517 ◽

Cited By ~ 1

Author(s):

Kai Wang ◽

Yu Su ◽

Yuting Liang ◽

Yanhui Song ◽

Liping Wang

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Blood Glucose ◽

Enzymatic Activity ◽

Glucose Levels ◽

Blood Glucose Levels

Type 2 diabetes mellitus (T2DM) is associated with pancreatic β-cell dysfunction which can be induced by oxidative stress. Deuterohemin-βAla-His-Thr-Val-Glu-Lys (DhHP-6) is a microperoxidase mimetic that can scavenge reactive oxygen species (ROS) in vivo. In our previous studies, we demonstrated an increased stability of linear peptides upon their covalent attachment to porphyrins. In this study, we assessed the utility of DhHP-6 as an oral anti-diabetic drug in vitro and in vivo. DhHP-6 showed high resistance to proteolytic degradation in vitro and in vivo. The degraded DhHP-6 product in gastrointestinal (GI) fluid retained the enzymatic activity of DhHP-6, but displayed a higher permeability coefficient. DhHP-6 protected against the cell damage induced by H2O2 and promoted insulin secretion in INS-1 cells. In the T2DM model, DhHP-6 reduced blood glucose levels and facilitated the recovery of blood lipid disorders. DhHP-6 also mitigated both insulin resistance and glucose tolerance. Most importantly, DhHP-6 promoted the recovery of damaged pancreas islets. These findings suggest that DhHP-6 in physiological environments has high stability against enzymatic degradation and maintains enzymatic activity. As DhHP-6 lowered the fasting blood glucose levels of T2DM mice, it thus represents a promising candidate for oral administration and clinical therapy.

Download Full-text

Gynura divaricata Effect on Blood Glucose Levels in Alloxan-Induced Diabetes Mice

Proceedings of the Singapore National Academy of Science ◽

10.1142/s2591722619400039 ◽

2019 ◽

Vol 13 (01) ◽

pp. 27-33

Author(s):

Evi Sovia ◽

Dian Anggraeny ◽

Ris Kristiana ◽

Firhan Hamdi Maulida ◽

Mutia Susparini

Keyword(s):

Blood Glucose ◽

Histological Examination ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Antidiabetic Activity ◽

Phytochemical Analysis ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Diabetes Mice ◽

Before And After

Gynura divaricata (GD) also known as Dewa leaf is one of the Indonesian medicinal plants which are also native to India, Thailand and China. GD is widely used as antidiabetic, antihypertensive and other diseases including several tumors. However, research on the effects of GD as antidiabetic is still not widely practiced, especially in Indonesia. The aim of this research is to investigate the effect of GD ethanolic extract on blood glucose levels of alloxan-induced diabetes mice. This research is an experimental research with pre- and post-test design using 24 male DDY strain mice. Animals experiment is divided into four groups, that are one normal group and three alloxan-induced diabetes mice group that give 1% CMC (control), 400[Formula: see text]mg/kg GD ethanolic extract and 0.65[Formula: see text]mg/kg glibenclamide, respectively. Treatment was given for 14 days. Blood glucose levels were measured before and after inducing alloxan, and after 14 days treatment. At the end of study, all of animals experiment were sacrificed for histological examination. Phytochemical analysis revealed that polyphenols, flavonoids, steroids, triterpenoids, terpenoids and and quinones were present in ethanol extract of GD. The results of this study showed significant ([Formula: see text]) decrease of blood glucose levels after GD ethanolic extract and glibenclamide treatment. The results of histological examination showed improvement in pancreas damage and significant increase in the number of beta cells in GD and glibenclamide groups. The results indicate that dewa leaf ethanolic extract has antidiabetic activity and the effectiveness is the same as glibenclamide. GD also could repair damage of pancreas caused by alloxan induction.

Download Full-text