scholarly journals Enterococci Isolated from Japanese Quails Exposed to Microgravity Conditions and Stability of their Properties

2009 ◽  
Vol 78 (2) ◽  
pp. 253-258 ◽  
Author(s):  
Andrea Lauková ◽  
Gabriela Michlovičová
Keyword(s):  
Lactic Acid Production ◽  
Long Term Storage ◽  
Japanese Quails ◽  
Before And After ◽  
Long Time ◽  
Microgravity Conditions ◽  
The Stability ◽  
Enterocin A ◽  
Long Storage

Enterococci isolated from the crop and caecum of Japanese quails exposed to 7 day conditions of microgravity were re-vitalized after their dry-freezing long storage. Originally, the strains were isolated from Japanese quails after their landing from flight onboard the orbital station Mir during the experiment in August 1990. Because taxonomy as well as the studies concerning the bacteriocins, especially those produced by enterococci, have been continually developed for years, the aim of this study was to confirm species identification, stability of the properties of enterococci as well as to test new properties after their long storage. Genotyping allotted the strains to the species E. faecium. Lactic acid production was detected in similar amounts in the strains before and after their long-storage in dry-frozen form. The strains were vancomycinsensitive and kanamycin-resistant before as well as after their long-time storage. Variability in sensitivity to different antibiotics was found among the strains tested even before and after longtime storage. Each of the strains possessed at least one structural enterocin gene. The structural genes for enterocin A, P, B, L50B were detected in E. faecium EP7. E. faecium EP2, EEP4 have the genes for ent A, B, L50B. The gene for ent P was detected only in the strain EP7. The most often detected was ent A gene followed by ent genes B, L50B. All strains inhibited growth of at least 4 out of 15 indicators. The stability of the enterococcal properties determined before as well as after their dry-freezing was not influenced during their long-term storage; moreover, new properties were determined.

The Carbonate-Hardening Lime Construction Material Properties Formation during their Long-Term Storage and Use under Normal Conditions

2019 ◽  
Vol 974 ◽  
pp. 187-194 ◽  
Author(s):  
Nikolay V. Lyubomirskiy ◽  
Tamara A. Bakhtina ◽  
Alexander S. Bakhtin ◽  
Sergey I. Fedorkin
Keyword(s):  
Construction Material ◽  
Construction Materials ◽  
Materials Properties ◽  
Long Term Storage ◽  
Efficiency Assessment ◽  
The Stability ◽  
Term Storage ◽  
Long Storage ◽  
Quality Construction

This paper presents the lime binding forced carbonate-hardening materials properties formation study and determins the stability of these properties during long-term storage and use under normal conditions. The tests showed these materials stability properties over time, confirming the strength and density growth of the test samples after long storage due to the calcium hydroxide recrystallization completion into calcium carbonate processes. Also, the results of the samples carbonate hardening study under natural conditions during 18 months are presented. An efficiency assessment of forced carbonate hardening as one of the methods of recycling technogenic CO2 in order to reduce its emissions in the atmosphere, and, in the result, to obtain high-quality construction materials has been made.

scholarly journals EFFECT OF FREEZING ON THE MILK QUALITY OF COWS

2020 ◽  
pp. 54-57
Author(s):  
Lyubov Valentinovna Efimova ◽  
Tatyana Vyacheslavovna Zaznobina ◽  
Elena Vladimirovna Gatilova ◽  
Olga Valeryevna Ivanova
Keyword(s):  
Quality Indicators ◽  
Age Groups ◽  
Milk Quality ◽  
Long Term Storage ◽  
Before And After ◽  
Frozen State ◽  
Term Storage ◽  
Two Age Groups

Due to the fact that milk has a short shelf life, the issue of its freezing and long-term storage in the frozen state is relevant. A study was conducted on the effect of freezing on the milk quality after its defrosting of cows-mothers and cows-daughters of Red-Motley breed. As a result, it was established how milk quality indicators to change after five months of storage in a frozen state in cows of two age groups. A high correlation was established between quality indicators before and after freezing. The influence of the “freezing” factor on milk quality indicators was recognized statistically significant, the “generation” factor – not significant.

Numerical Simulation on Deformation Features and Filling Efficiency of Channel Goaf Foundation

2013 ◽  
Vol 353-356 ◽  
pp. 3707-3712
Author(s):  
Zhi Dong Zhou ◽  
Hong Bo Zhang ◽  
Xiu Guang Song ◽  
Hong Ya Yue
Keyword(s):  
Numerical Simulation ◽  
Comparative Analysis ◽  
Comprehensive Analysis ◽  
Obvious Effect ◽  
Before And After ◽  
Check Gate ◽  
Deformation Features ◽  
Natural Foundation ◽  
The Stability

For analyzing and evaluating the deformation features of channel goaf foundation and its effect on superstructure, according to the foundation reinforcement engineering of Ji-Liang Channel Check Gate, the comparative analysis on the differential settlement between the unfavorable foundation and natural foundation was performed by numerical simulation with FLAC3D. The computer results showed that the present foundation had obvious effect on the stability and security of the superstructure, so the engineering treatment are needed. Based on technical-economic comprehensive analysis among three treatment methods for strengthening the channel golf foundation with different kinds of filling, the reasonable method is put forward in this paper. By comparing mechanical characteristic of flashboard in channel goaf before and after strengthening, we confirmed the foundation stability of channel goaf and the security of the ground building which can satisfy the long term usage requirements.

scholarly journals Stability of Glucose Concentrations in Frozen Plasma

2020 ◽  
pp. 193229682096365
Author(s):  
Stefan Pleus ◽  
Guido Freckmann ◽  
Annette Baumstark ◽  
Cornelia Haug
Keyword(s):  
Venous Blood ◽  
Relative Difference ◽  
Plasma Samples ◽  
Conflicting Information ◽  
Long Term Storage ◽  
The Stability ◽  
Relative Differences ◽  
Frozen Plasma ◽  
Laboratory Analyzer

Background: Conflicting information is available regarding the stability of glucose concentrations in frozen plasma samples. Clinical trials could benefit from such long-term storage because it would allow usage of a central laboratory with higher-quality laboratory analyzers in contrast to mobile analyzers in a decentralized setting. Methods: In this study, venous blood samples were collected in lithium-heparin gel tubes. Plasma was separated immediately after blood was drawn, and from each of the 21 plasma samples, 6 aliquots were prepared for measurement at 6 time points: immediately and after 2, 4, 6, 8, and 12 weeks. Between sampling and measurement, aliquots were stored at less than −20°C. Transport on dry ice was simulated by placing aliquots in a −80°C freezer for 5 days between weeks 8 and 12. Measurements were performed on a hexokinase-based laboratory analyzer. Average relative differences and corresponding 99% confidence intervals (CIs) were calculated between the stored aliquots’ and the immediately measured aliquots’ glucose concentrations. Glucose concentrations were deemed stable as long as average relative differences were ≤±2.5%. Results: Over the whole 12-weeks duration, the largest average relative difference was −1.82% (99% CI: –2.25% to −1.39%). Shorter storage durations tended to lead to less bias. Conclusion: In this study, the stability of glucose concentrations in frozen plasma samples obtained with lithium-heparin gel tubes could be shown for up to 12 weeks. Future studies should be performed to assess whether this is independent of the glucose analyzer and the type of sampling tube used.

Stability and epimerisation behaviour of ergot alkaloids in various solvents

2008 ◽  
Vol 1 (1) ◽  
pp. 67-78 ◽  
Author(s):  
M. Hafner ◽  
M. Sulyok ◽  
R. Schuhmacher ◽  
C. Crews ◽  
R. Krska
Keyword(s):  
Degradation Products ◽  
Ergot Alkaloids ◽  
Carbonate Buffer ◽  
Long Term Storage ◽  
No Formation ◽  
Temperature Levels ◽  
The Stability ◽  
Extraction Mixture ◽  
Term Storage

In this paper the stability and degree of epimerisation of six major ergot alkaloids at three different temperature levels (-20 °C, +4 °C and +20 °C) over periods of 18 hours and six weeks is reported for the first time. The behaviour of ergometrine, ergocornine, ergocristine, α-ergocryptine, ergosine and ergotamine was thoroughly studied in seven solvents which are employed for the preparation of calibrants and extraction mixtures, respectively. Moreover, the stability of the ergot alkaloids was tested in different cereal extracts (rye, wheat, barley, oats) for 1, 2 and 6 days. Of the toxins tested, the ergopeptide-type toxins ergosine, ergotamine, ergocornine, α-ergocryptine and ergocristine showed similar behaviour patterns. The simple lysergic acid derivative ergometrine was more stable and showed hardly any epimerisation to ergometrinine, with the sum of both epimers remaining constant in all seven solvents. The ergopeptides tested show variable epimerisation tendencies, and were also less stable during six weeks at 20 °C. Ergosine showed the highest degree of epimerisation (43% after 6 weeks at 20 °C). In general, the order of epimerisation promotion was methanol/dichloromethane > acetonitrile/buffer > extraction mix > stabilising solution > acetonitrile >> chloroform. Long-term storage at room temperature can only be carried out in chloroform, which showed no epimerisation for all toxins even at 20 °C and also kept the sum of R and S forms constant, which indicates no formation of aci-epimers or other degradation products. Long-term storage of ergot alkaloids in acetonitrile, the most convenient solvent with respect to HPLC analysis, should be carried out at temperatures of -20 °C or below. The constant epimer ratio of all ergot alkaloids in the extraction mixture acetonitrile/ammonium carbonate buffer (200 mg/l; 92:8, v/v) during an HPLC run (18 hours) demonstrates the stability of the toxins in this extraction mixture.

scholarly journals Does long-term storage of clay samples influence their mechanical characteristics?

2020 ◽  
Vol 57 (2) ◽  
pp. 304-309 ◽  
Author(s):  
Mustapha Abdellaziz ◽  
Mahmoud N. Hussien ◽  
Mohamed Chekired ◽  
Mourad Karray
Keyword(s):  
Mechanical Characteristics ◽  
Long Term Storage ◽  
Temperature And Humidity ◽  
Before And After ◽  
Prime Objective ◽  
Term Storage ◽  
Physical And Mechanical Characteristics

The prime objective of this study is to assess the influence of long-term storage on the physical and mechanical characteristics of clay samples. Samples from two different clays were sealed and stored in a temperature- and humidity-controlled room at the geotechnical laboratory of the Université de Sherbrooke for up to 27 years. The stored clay samples were tested before and after long-term storage and the results compared in this note. The comparison showed that even with long-term storage, the majority of the physical and mechanical characteristics of the samples were preserved.

Low selenocentric orbits stability analysis

2020 ◽  
Author(s):  
Chongrui Du ◽  
O.L. Starinova
Keyword(s):  
Rate Of Change ◽  
The Sun ◽  
Space Systems ◽  
The Moon ◽  
Orbital Structure ◽  
Motion Modeling ◽  
The Earth ◽  
Long Time ◽  
The Stability

The tasks of studying the Moon require long-term functioning space systems. Most of the low selenocentric orbits are known to be unstable, which requires a propellant to maintain the orbital structure. For these orbits, the main disturbing factors are the off-center gravitational field of the Moon and the gravity of the Earth and the Sun. This paper analyzes the stability of low selenocentric orbits according to passive motion modeling and takes into account these main disturbing factors. We put forward a criterion for determining the stability of the orbit and used it to analyze the circular orbit of the Moon at an altitude of 100 kilometers. According to different initial data and different dates, we obtained ranges of the Moon’s orbits with good stability. At the same time, we analyzed the rate of change in the longitude of the ascending node, and found a stable low lunar orbit which can operate for a long time.

Synthesis Optimization of Guanidinium Dinitramide (GDN)

2020 ◽  
Vol 20 (11) ◽  
pp. 6855-6861
Author(s):  
Wooram Kim ◽  
Mijeong Park ◽  
Jong-Ki Jeon ◽  
Youngmin Jo
Keyword(s):  
Maximum Yield ◽  
Resonance Structure ◽  
Kcal Mole ◽  
Additional Advantage ◽  
Long Term Storage ◽  
Ft Ir ◽  
The Stability ◽  
Infrared Frequencies ◽  
Term Storage

Dinitramide anion [−N(NO2)2] salt composed of resonance structure is a plausible oxidizing agents, as efficient propellant. Among them, guanidinium dinitramide (GDN) is an organic compound improving the stability against moisture, as well long term storage. An additional advantage composed guanidinium ion is the reaction efficient via the decomposed by-product during pyrognostics, maximum yield of 99%. The types of GDN (GDN-I, II, III, IV, V) were synthesized using several starting material such as guanidine acetate, chloride, carbonate, nitrate and sulfate under hydrodeprivation. In this work, the intermediates formed in these processes were closely identified and their thermal properties, and chemical structure were examined. The absorption peaks by Fourier transform infrared (FT-IR) were found guanidinium infrared frequencies (3452, 3402, 3354, 3278, 1642 cm−1) and dinitramide infrared frequencies (3208, 1570, 1492, 1416, 1337, 1179, 1000 cm−1). The activation energy of GDN samples were obtained Ea = 53.26 Kcal/mole (GDN-I), 50.94 Kcal/mole (GDN-II), 52.34 Kcal/mole (GDN-III), 62.19 Kcal/mole (GDN-IV), 55.32 Kcal/mole (GDN-V) from exothermic at over 153°C.

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