BIOREMEDIATION OF CRUDE OIL CONTAMINATED SEAWATER WITH THE APPLICATION OF BIOSURFACTANT AND BIOSTIMULATION

Marine oil spills have bad impacts on the marine biota. Oil spill mitigation that is currently safe, effi cient, relatively cheap and easy to implement is bioremediation, that is degradation of oil spills biologically using microorganisms. Petroleum will be more easily dispersed in water when surfactants are added. The surfactants have the ability to increase the bioavailability of petroleum to facilitate bacteria contact with carbon sources as their feed. This study was intended to test the effect of addition of diethanolamide (DEA) surfactants to improve the ability of bacteria to degrade hydrocarbon compound in the seawater media. The biodegradation experiment was conducted in 8-liter seawater media and the ability of DEA surfactants to reduce surface tension, oil content, pH and nutrients on days 0, 1, 3, 6 and 10 were observed. GC-MS analysis was conducted to detect chemical component changes in petroleum. A bacterial consortium of Enterobacter sp., Pseudomonas sp., and Raoultella sp. was utilized. The oil was degraded up to 65.52% with biodegradation rate k = -0.1054 t in the media added with DEA surfactants. The aliphatic fraction detected was C17-C31 n-alkane compound and after biodegradation it became C20- C31. The results showed that DEA surfactants were able to improve the ability of bacterial consortium to degrade petroleum.

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Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons

Water ◽

10.3390/w13010066 ◽

2020 ◽

Vol 13 (1) ◽

pp. 66

Author(s):

Rafaela Perdigão ◽

C. Marisa R. Almeida ◽

Filipa Santos ◽

Maria F. Carvalho ◽

Ana P. Mucha

Keyword(s):

Petroleum Hydrocarbons ◽

Oil Spills ◽

Carbon Sources ◽

Bacterial Biomass ◽

Bacterial Consortium ◽

Growth Conditions ◽

Natural Seawater ◽

Bacterial Strains ◽

Beach Sediments ◽

Insight Into

Oil spill pollution remains a serious concern in marine environments and the development of effective oil bioremediation techniques are vital. This work is aimed at developing an autochthonous hydrocarbon-degrading consortium with bacterial strains with high potential for hydrocarbons degradation, optimizing first the growth conditions for the consortium, and then testing its hydrocarbon-degrading performance in microcosm bioremediation experiments. Bacterial strains, previously isolated from a sediment and cryopreserved in a georeferenced microbial bank, belonged to the genera Pseudomonas, Rhodococcus and Acinetobacter. Microcosms were assembled with natural seawater and petroleum, for testing: natural attenuation (NA); biostimulation (BS) (nutrients addition); bioaugmentation with inoculum pre-grown in petroleum (BA/P) and bioaugmentation with inoculum pre-grown in acetate (BA/A). After 15 days, a clear blending of petroleum with seawater was observed in BS, BA/P and BA/A but not in NA. Acetate was the best substrate for consortium growth. BA/A showed the highest hydrocarbons degradation (66%). All bacterial strains added as inoculum were recovered at the end of the experiment. This study provides an insight into the capacity of autochthonous communities to degrade hydrocarbons and on the use of alternative carbon sources for bacterial biomass growth for the development of bioremediation products to respond to oil spills.

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Marine Oil Spills 2018

Journal of Marine Science and Engineering ◽

10.3390/jmse7040082 ◽

2019 ◽

Vol 7 (4) ◽

pp. 82 ◽

Cited By ~ 2

Author(s):

Merv Fingas

Keyword(s):

Oil Spills ◽

Marine Oil ◽

The Public ◽

The Media ◽

Marine Oil Spills

Major oil spills can attract the attention of the public and the media [...]

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Prospection of Fungal Lignocellulolytic Enzymes Produced from Jatoba (Hymenaea courbaril) and Tamarind (Tamarindus indica) Seeds: Scaling for Bioreactor and Saccharification Profile of Sugarcane Bagasse

Microorganisms ◽

10.3390/microorganisms9030533 ◽

2021 ◽

Vol 9 (3) ◽

pp. 533

Author(s):

Alex Graça Contato ◽

Tássio Brito de Oliveira ◽

Guilherme Mauro Aranha ◽

Emanuelle Neiverth de Freitas ◽

Ana Claudia Vici ◽

...

Keyword(s):

Carbon Sources ◽

Temporal Analysis ◽

Industrial Applications ◽

Lignocellulolytic Enzymes ◽

Tamarindus Indica ◽

Acetyl Xylan Esterase ◽

Trametes Hirsuta ◽

Hymenaea Courbaril ◽

The Media ◽

Main Components

The lignocellulosic biomass comprises three main components: cellulose, hemicellulose, and lignin. Degradation and conversion of these three components are attractive to biotechnology. This study aimed to prospect fungal lignocellulolytic enzymes with potential industrial applications, produced through a temporal analysis using Hymenaea courbaril and Tamarindus indica seeds as carbon sources. α-L-arabinofuranosidase, acetyl xylan esterase, endo-1,5-α-L-arabinanase, β-D-galactosidase, β-D-glucosidase, β-glucanase, β-D-xylosidase, cellobiohydrolase, endoglucanase, lichenase, mannanase, polygalacturonase, endo-1,4-β-xylanase, and xyloglucanase activities were determined. The enzymes were produced for eight filamentous fungi: Aspergillus fumigatus, Trametes hirsuta, Lasiodiplodia sp., two strains of Trichoderma longibrachiatum, Neocosmospora perseae, Fusarium sp. and Thermothelomyces thermophilus. The best producers concerning enzymatic activity were T. thermophilus and T. longibrachiatum. The optimal conditions for enzyme production were the media supplemented with tamarind seeds, under agitation, for 72 h. This analysis was essential to demonstrate that cultivation conditions, static and under agitation, exert strong influences on the production of several enzymes produced by different fungi. The kind of sugarcane, pretreatment used, microorganisms, and carbon sources proved limiting sugar profile factors.

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Comparative Proteomics of Marinobacter sp. TT1 Reveals Corexit Impacts on Hydrocarbon Metabolism, Chemotactic Motility, and Biofilm Formation

Microorganisms ◽

10.3390/microorganisms9010003 ◽

2020 ◽

Vol 9 (1) ◽

pp. 3

Author(s):

Saskia Rughöft ◽

Nico Jehmlich ◽

Tony Gutierrez ◽

Sara Kleindienst

Keyword(s):

Biofilm Formation ◽

Oil Spills ◽

Carbon Sources ◽

Comparative Proteomics ◽

Hydrocarbon Biodegradation ◽

Solvent Tolerance ◽

Marine Microorganisms ◽

Degrading Bacteria ◽

Oil Spill Response ◽

First Time

The application of chemical dispersants during marine oil spills can affect the community composition and activity of marine microorganisms. Several studies have indicated that certain marine hydrocarbon-degrading bacteria, such as Marinobacter spp., can be inhibited by chemical dispersants, resulting in lower abundances and/or reduced biodegradation rates. However, a major knowledge gap exists regarding the mechanisms underlying these physiological effects. Here, we performed comparative proteomics of the Deepwater Horizon isolate Marinobacter sp. TT1 grown under different conditions. Strain TT1 received different carbon sources (pyruvate vs. n-hexadecane) with and without added dispersant (Corexit EC9500A). Additional treatments contained crude oil in the form of a water-accommodated fraction (WAF) or chemically-enhanced WAF (CEWAF; with Corexit). For the first time, we identified the proteins associated with alkane metabolism and alginate biosynthesis in strain TT1, report on its potential for aromatic hydrocarbon biodegradation and present a protein-based proposed metabolism of Corexit components as carbon substrates. Our findings revealed that Corexit exposure affects hydrocarbon metabolism, chemotactic motility, biofilm formation, and induces solvent tolerance mechanisms, like efflux pumps, in strain TT1. This study provides novel insights into dispersant impacts on microbial hydrocarbon degraders that should be taken into consideration for future oil spill response actions.

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Response to marine oil spills

Marine Pollution Bulletin ◽

10.1016/0025-326x(88)90607-8 ◽

1988 ◽

Vol 19 (6) ◽

pp. 297

Author(s):

R.A.A. Blackman

Keyword(s):

Oil Spills ◽

Marine Oil ◽

Marine Oil Spills

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Remote Sensing Monitoring of Marine Oil Spills

Information Engineering of Emergency Treatment for Marine Oil Spill Accidents ◽

10.1201/9780429289101-2 ◽

2019 ◽

pp. 31-67

Author(s):

Lin Mu ◽

Lizhe Wang ◽

Jining Yan

Keyword(s):

Remote Sensing ◽

Oil Spills ◽

Marine Oil ◽

Remote Sensing Monitoring ◽

Marine Oil Spills

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The application of a carrier-based bioremediation strategy for marine oil spills

Marine Pollution Bulletin ◽

10.1016/j.marpolbul.2014.03.044 ◽

2014 ◽

Vol 84 (1-2) ◽

pp. 339-346 ◽

Cited By ~ 9

Author(s):

Petra J. Sheppard ◽

Keryn L. Simons ◽

Eric M. Adetutu ◽

Krishna K. Kadali ◽

Albert L. Juhasz ◽

...

Keyword(s):

Oil Spills ◽

Marine Oil ◽

Marine Oil Spills

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Sacrificial amphiphiles: Eco-friendly chemical herders as oil spill mitigation chemicals

Science Advances ◽

10.1126/sciadv.1400265 ◽

2015 ◽

Vol 1 (5) ◽

pp. e1400265 ◽

Cited By ~ 27

Author(s):

Deeksha Gupta ◽

Bivas Sarker ◽

Keith Thadikaran ◽

Vijay John ◽

Charles Maldarelli ◽

...

Keyword(s):

Crude Oil ◽

Oil Spill ◽

Oil Spills ◽

Cold Water ◽

Marine Ecosystem ◽

Hot Water ◽

Sea Surface ◽

Marine Biota ◽

Polar Group ◽

Branched Chain

Crude oil spills are a major threat to marine biota and the environment. When light crude oil spills on water, it forms a thin layer that is difficult to clean by any methods of oil spill response. Under these circumstances, a special type of amphiphile termed as “chemical herder” is sprayed onto the water surrounding the spilled oil. The amphiphile forms a monomolecular layer on the water surface, reducing the air–sea surface tension and causing the oil slick to retract into a thick mass that can be burnt in situ. The current best-known chemical herders are chemically stable and nonbiodegradable, and hence remain in the marine ecosystem for years. We architect an eco-friendly, sacrificial, and effective green herder derived from the plant-based small-molecule phytol, which is abundant in the marine environment, as an alternative to the current chemical herders. Phytol consists of a regularly branched chain of isoprene units that form the hydrophobe of the amphiphile; the chain is esterified to cationic groups to form the polar group. The ester linkage is proximal to an allyl bond in phytol, which facilitates the hydrolysis of the amphiphile after adsorption to the sea surface into the phytol hydrophobic tail, which along with the unhydrolyzed herder, remains on the surface to maintain herding action, and the cationic group, which dissolves into the water column. Eventual degradation of the phytol tail and dilution of the cation make these sacrificial amphiphiles eco-friendly. The herding behavior of phytol-based amphiphiles is evaluated as a function of time, temperature, and water salinity to examine their versatility under different conditions, ranging from ice-cold water to hot water. The green chemical herder retracted oil slicks by up to ~500, 700, and 2500% at 5°, 20°, and 35°C, respectively, during the first 10 min of the experiment, which is on a par with the current best chemical herders in practice.

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Morphogenesis and Production of Enzymes byPenicillium echinulatumin Response to Different Carbon Sources

BioMed Research International ◽

10.1155/2014/254863 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 6

Author(s):

Willian Daniel Hahn Schneider ◽

Laísa dos Reis ◽

Marli Camassola ◽

Aldo José Pinheiro Dillon

Keyword(s):

Enzymatic Activity ◽

Sugar Cane ◽

Filter Paper ◽

Carbon Sources ◽

Sugar Cane Bagasse ◽

Xylanase Production ◽

Penicillium Echinulatum ◽

The Media ◽

Filter Paper Activity ◽

Second Generation Ethanol

The effect of different carbon sources on morphology and cellulase and xylanase production ofPenicillium echinulatumwas evaluated in this work. Among the six carbon sources studied, cellulose and sugar cane bagasse were the most suitable for the production of filter paper activity, endoglucanases, xylanases, andβ-glucosidases. However, sucrose and glucose showedβ-glucosidase activities similar to those obtained with the insoluble sources. The polyacrylamide gels proved the enzymatic activity, since different standards bands were detected in the media mentioned above. Regarding morphology, it was observed that the mycelium in a dispersed form provided the greatest enzymatic activity, possibly due to greater interaction between the substrate and hyphae. These data are important in understanding the physiology of fungi and could contribute to obtaining enzyme with potential application in the technology of second generation ethanol.

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