scholarly journals Phytoplankton and Bacterial Communities in South Harbour, Manila Bay, Philippines

2018 ◽  
Vol 35 (1-2) ◽  
pp. 107-113
Author(s):  
Rhodora V Azanza ◽  
Nero M Austero ◽  
Jenelle Clarisse R. Dungca ◽  
Frenchly Joyce O. Caspe ◽  
Lidita Khandeparker

In line with the ASEAN-India project “Extent of Transfer of Alien Invasive Organisms in South/Southeast Asia via Shipping”, phytoplankton and bacterial communities in the waters off South Harbour, Manila Bay were investigated. Sampling was done in July and August 2012 and in April and May 2013. A total of 67 phytoplankton species including 29 diatoms and 38 dinoflagellates were identified. Potentially toxic Pseudo-nitzschia spp. were among the diatoms found as well as dinoflagellates Alexandrium spp., and Gymnodinium spp. The diatom Skeletonema costatum appeared to be the dominant species in July and August 2012, whereas Chaetoceros spp. constituted over 85% of the total phytoplankton assemblage in April and May 2013. Mean bacterialabundance ranged from 9.53 x 102–3.18 x 105 cells/mL in July 2012. In addition, 93 bacterial isolates were  identified using 16S rDNA, several of which belonged to the following phyla: Actinobacteria, Bacteriodetes,  Firmicutes, and Proteobacteria; whereas, others were determined as uncultured bacterial clones. These results will serve as a valuable baseline for future studies on phytoplankton and bacterial community structure in Manila Bay.

Author(s):  
Richard F. Lee ◽  
Judd C. Nevenzel ◽  
G.-A. Paffenhöfer ◽  
A.A. Benson ◽  
Stuart Patton ◽  
...  

2007 ◽  
Vol 73 (17) ◽  
pp. 5683-5686 ◽  
Author(s):  
Dana M. Cook ◽  
Emily DeCrescenzo Henriksen ◽  
Rima Upchurch ◽  
Joy B. Doran Peterson

ABSTRACT The Tipula abdominalis larval hindgut microbial community presumably facilitates digestion of the lignocellulosic diet. The microbial community was investigated through characterization of bacterial isolates and analysis of 16S rRNA gene clone libraries. This initial study revealed novel bacteria and provides a framework for future studies of this symbiosis.


1978 ◽  
Vol 62 (6) ◽  
pp. 987-990 ◽  
Author(s):  
Juan L. Serra ◽  
Maria J. Llama ◽  
Eduardo Cadenas

2018 ◽  
Vol 47 (2) ◽  
pp. 74-78
Author(s):  
Ana Lídia Soares COTA ◽  
Ronaldo Gomes ALVIM

Abstract Introduction Proper storage conditions and maintenance of viable biological material plays an important role in microbiological research, allowing for the opportunity to conduct future studies. Objective To evaluate the viability of Streptococcus mutans strains that were previously grown and stored under different temperatures for approximately eight years. Material and method In this study, we evaluated 393 bacterial isolates that were stored in a freezer at -80°C (G1) and 200 isolates stored in a freezer at -20°C (G2). Aliquots of each sample were plated on blood agar and mitis-salivarius bacitracin sucrose agar-solidified medium. After incubating under microaerophilic conditions in an incubator at 37°C for 72 hours, the presence, morphology and purity of bacterial growth was observed. The data were analyzed by means of descriptive statistics. Result Microbial viability was observed in almost all samples (99.7%) in G1, whereas all isolates stored at -20°C were considered inviable. Conclusion The viability of S. mutans is influenced by the storage temperature of the samples, and the strains remain viable when stored under ideal temperature conditions (-80°C), even when stored for a long period of time.


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