scholarly journals Staining criteria, collection and number of cells evaluated to identify micronuclei in buccal mucosa cells of agricultural workers exposed to pesticides.

2021 ◽  
Vol 9 (18) ◽  
pp. 34-38
Author(s):  
Dayna Santiago-Manzano
Keyword(s):  
Systematic Review ◽  
Oral Mucosa ◽  
Buccal Mucosa ◽  
The Internet ◽  
Buccal Cells ◽  
Control Groups ◽  
Buccal Mucosa Cells ◽  
Criteria For Evaluation ◽  
Number Of Cells ◽  
Exfoliated Buccal Cells

Although there are several publications that refer to the basic criteria established for the micronucleus (MN) test in exfoliated buccal cells, there is still a difference in the quantity, method of obtention and staining of the cells for the evaluation of the presence of micronuclei. Objective. Identify the criteria for evaluation of MN in oral mucosa cells exposed to pesticide in investigations carried out. Material and methods. A systematic review was carried out on the internet, based on articles published in Crossref, JCR, Scopus, PubMed, Google academic, using keywords such as: micronuclei, buccal mucosa cells, genotoxic damage, pesticides and biomarker. Results. In the six selected articles, four presented statistically significant values ​​with the presence of MN when comparing the exposed groups with respect to the control groups, and the criteria for staining, collection and number of cells evaluated to identify micronuclei were very varied. Conclusions. It is important to follow validated and standardized protocols for the MN oral cytoma assay. Being considered in all the parameters suggested in the protocol will increase the reliability of the studies and will give the possibility of comparing the results obtained.

scholarly journals Micronucleus Frequency in Buccal Cells of Males Exposed to Air Pollution in Kufa City

2018 ◽  
Vol 28 (2) ◽  
pp. 43 ◽  
Author(s):  
Farha A. Ali Shafi
Keyword(s):  
Buccal Mucosa ◽  
Control Group ◽  
Buccal Cells ◽  
Control Groups ◽  
Cement Factory ◽  
Buccal Mucosa Cells ◽  
Significant Difference ◽  
Genomic Damage ◽  
The Mean ◽  
And Control

The aim of the present study is to explore micronuclei recurrence, as a biomarker of genomic damage in buccal cells of individuals living in polluted areas located near to the cement factory in Kufa city. Twenty four apparently healthy males residing in Kufa are enrolled in the present study, their ages between 18-30 years, as control group 24 males with same age are enrolled liv-ing in the center of Najaf city a far from cement factory. A sample of the exposed and control groups of buccal mucosa cells was collected during Spring months in 2016. The mean count of cell with micronuclei in buccal cells of the exposed group and control were (22.33 ± 0.97; 11.583 ± 0.85) respectively, the mean count of the binucleated cells of the exposed and control groups were (7.208 ± 0.71, 10.041 ± 0.84) respectively, a significant difference was detected in the ob-served frequencies of micronuclei and binnucleated cells in the buccal mucosa cells between the exposed and control group. The results indicated that exposure to air pollutants related to cement production induce cytotoxic as well as genotoxic damage in buccal mucosa cells

Clinical application of micronucleus test in exfoliated buccal cells: A systematic review and metanalysis

2015 ◽  
Vol 766 ◽  
pp. 20-31 ◽  
Author(s):  
Claudia Bolognesi ◽  
Stefano Bonassi ◽  
Siegfried Knasmueller ◽  
Michael Fenech ◽  
Marco Bruzzone ◽  
...  
Keyword(s):  
Systematic Review ◽  
Clinical Application ◽  
Micronucleus Test ◽  
Buccal Cells ◽  
Exfoliated Buccal Cells

scholarly journals Micronucleus frequency in buccal mucosa cells of patients with neurodegenerative diseases

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hauke Reimann ◽  
Helga Stopper ◽  
Thomas Polak ◽  
Martin Lauer ◽  
Martin J. Herrmann ◽  
...  
Keyword(s):  
Dna Damage ◽  
Neurodegenerative Diseases ◽  
Buccal Mucosa ◽  
Lifestyle Factors ◽  
Direct Link ◽  
Buccal Cells ◽  
Micronucleus Frequency ◽  
Binucleated Cells ◽  
Buccal Mucosa Cells ◽  
Healthy Participants

AbstractNeurodegenerative diseases show an increase in prevalence and incidence, with the most prominent example being Alzheimer’s disease. DNA damage has been suggested to play a role in the pathogenesis, but the exact mechanisms remain elusive. We enrolled 425 participants with and without neurodegenerative diseases and analyzed DNA damage in the form of micronuclei in buccal mucosa samples. In addition, other parameters such as binucleated cells, karyolytic cells, and karyorrhectic cells were quantified. No relevant differences in DNA damage and cytotoxicity markers were observed in patients compared to healthy participants. Furthermore, other parameters such as lifestyle factors and diseases were also investigated. Overall, this study could not identify a direct link between changes in buccal cells and neurogenerative diseases, but highlights the influence of lifestyle factors and diseases on the human buccal cytome.

scholarly journals Micronuclei and Other Nuclear Anomalies in Exfoliated Buccal Mucosa Cells in Breast Cancer

2021 ◽  
Vol 5 (3) ◽  
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Genomic Instability ◽  
Buccal Mucosa ◽  
Control Group ◽  
Breast Cancer Patients ◽  
Buccal Cells ◽  
Healthy Women ◽  
Buccal Mucosa Cells ◽  
Cytome Assay

Objectives: This study designed to assess the genomic instability between healthy women and women with breast cancer by means of buccal cells micronucleus (MN) cytome assay. Methods: The current study comprised 25 healthy women and 30 breast cancer patients. The exfoliated cells of buccal mucosa were taken after each participants rinse their mouths with tap water. The micronucleated cell and nuclear anomalies were analyzed under a total magnification of X1000, 2000 cells per subjects ( patient and control group) were scored and the frequencies of nuclear anomalies including MN, binucleates (BN), Pycnotic cell, karyolysis (KL) and karyorrhexis (KR) were evaluated in exfoliated buccal mucosa cells of women with primary BC and healthy women. Results: The frequencies of micronuclei and all nuclear anomalies in buccal cells of BC patients were significantly increased compared with the controls. (For Binucleates cells only, p<0.001; in all other cases, P < 0.0001). The mean scores of micronuclei and all nuclear anomalies for the breast cancer patients were (10.66±0.3845, 6.20±0.26, 8.40±0. 22, 18.40±0. 34, 19.13 ±0.40) were significantly higher than that of healthy women). Conclusion: Elevated frequency of micronucleated cells and all nuclear anomalies in the buccal mucosa of breast cancer patients reveal the genomic instability. These findings propose that the buccal MN-cytome assay can be used to measure both genotoxic and cytotoxic effects in primary cancer patients.

scholarly journals Chromosomal Damage and Apoptosis in Exfoliated Buccal Cells from Individuals with Oral Cancer

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lavínia Tércia Magalhães Dórea ◽  
José Roberto Cardoso Meireles ◽  
Júlia Paula Ramos Lessa ◽  
Márcio Campos Oliveira ◽  
Carlos Alberto de Bragança Pereira ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Buccal Mucosa ◽  
Chromosome Damage ◽  
Chromosomal Damage ◽  
Buccal Cells ◽  
Exfoliated Cells ◽  
Cytological Abnormalities ◽  
And Control ◽  
Exfoliated Buccal Cells ◽  
In Cells

This study aimed to investigate cytological abnormalities indicative of chromosome damage (micronuclei) and apoptosis (karyorrhexis, pyknosis, and condensed chromatin) in exfoliated cells from the buccal mucosa of patients with oral cancer and control subjects. The sample included twenty individuals with oral cancer and forty individuals with normal buccal mucosa. Material was collected from the cheek epithelium in areas with lesions and areas without abnormalities. A minimum of one thousand cells was analyzed. Micronuclei were found significantly more frequently in cells collected from lesions than in cells from normal areas, independent of the presence/absence of cancer (P<0.0001). They were also significantly more frequent in smokers and in mouthwash users (P<0.0001). Apoptosis occurred significantly less frequently in individuals with oral cancer (P<0.0001). These results show that oral cancer is associated with higher frequency of chromosomal damage and suggest that apoptosis is compromised in the buccal cells of individuals with this kind of neoplasia.

Biomonitoring of Buccal Mucosa Cells in Chronic Smokers and Nonsmokers

2016 ◽  
Vol 7 (4) ◽  
pp. 189-194 ◽  
Author(s):  
Neeraj Taneja ◽  
Ruhi S Sarpal ◽  
Navdeep K Shergill ◽  
SV Ravindra
Keyword(s):  
Buccal Mucosa ◽  
Tobacco Consumption ◽  
Contributing Factors ◽  
Control Groups ◽  
Significant Relation ◽  
Related Factors ◽  
Buccal Mucosa Cells ◽  
Study Case ◽  
And Control ◽  
Male Subjects

ABSTRACT Background Despite the well-known presence of carcinogens in tobacco smoke, results in the scientific literature linking smoking habits to micronuclei frequency are rather controversial. Aim The study was conducted to compare and evaluate the frequency of micronuclei in chronic smokers and nonsmokers in relation to habit history, personal history, and other related factors like occupation, exposure to radiation, etc. Materials and methods A total of 50 randomly selected male subjects were included in the study. Case and control groups (smokers and nonsmokers respectively) comprised 25 subjects each (mean age in controls = 38.24 ± 2.7; mean age in smokers = 39.32 ± 3.8). Results and conclusion There was a significant relation between tobacco consumption and frequency of micronucleated cells (p = 0.05) and between radiation exposure and presence of micronuclei (p = 0.05) in controls and subjects. Within the smokers group, left cheek scrapings showed higher count (p = 0.05; significant) for the micronucleated cells as compared with right cheek scrapings. Clinical significance In this study, an attempt was made to estimate the cytogenetic damage in oral mucosa in people habituated to smoking beedi. Though tobacco plays an important role in micronuclei generation, other factors like ionizing radiation and personal habits also contribute to micronuclei frequency. Site of smear, sample size, nuclear specific stain usage are some of the contributing factors. In addition, cytogenetic alterations like karyolysis, pyknosis, etc., can be included in future studies to increase the specificity. How to cite this article Sarpal RS, Taneja N, Shergill NK, Ravindra SV. Biomonitoring of Buccal Mucosa Cells in Chronic Smokers and Nonsmokers. World J Dent 2016;7(4):189-194.

Cytogenetic Biomonitoring in Buccal Mucosa Cells from Young Smokers

2015 ◽  
Vol 59 (6) ◽  
pp. 474-478 ◽  
Author(s):  
Victor Hugo Pereira da Silva ◽  
Raquel de Luna Antonio ◽  
Sabine Pompeia ◽  
Daniel Araki Ribeiro
Keyword(s):  
Dna Damage ◽  
Oral Mucosa ◽  
Tobacco Smoke ◽  
Buccal Mucosa ◽  
Micronucleus Test ◽  
Non Invasive ◽  
Buccal Mucosa Cells ◽  
Young Smokers ◽  
Oral Cancer Risk ◽  
Nuclear Alterations

Objective: Nowadays, much attention has been focused on the search for new non-invasive methodologies able to predict malignant transformation of oral mucosa cells. The aim of the present study was to comparatively evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis and karyorrhexis) in exfoliated oral mucosa cells from smokers and non-smokers in buccal mucosa cells. Study Design: A total of 24 young, healthy smokers and 14 non-smokers were included in this setting. Individuals had epithelial cells from the cheek mechanically exfoliated, placed in fixative and dropped in clean slides which were checked for the above nuclear phenotypes. Results: Smokers presented more (p < 0.05) micronucleated oral mucosa cells than non-smokers. Tobacco smoke was not able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis and karyolysis. Conclusion: In summary, these data indicate that the cigarette is able to induce micronuclei in oral mucosa cells, so the micronucleus test is a suitable method for predicting oral cancer risk.

scholarly journals Effect of photobiomodulation in secondary intention gingival wound healing—a systematic review and meta-analysis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Pooya Ebrahimi ◽  
Mahdi Hadilou ◽  
Ferdos Naserneysari ◽  
Amirmohammad Dolatabadi ◽  
Rana Tarzemany ◽  
...  
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Clinical Trials ◽  
Randomized Clinical Trials ◽  
Meta Analysis ◽  
Healing Process ◽  
Adjunctive Treatment ◽  
Control Groups ◽  
Secondary Intention ◽  
Post Operative Pain

Abstract Background Photobiomodulation is widely being used to improve the wound healing process in dentistry and a vast majority of studies have proven its benefits. But there are plenty of knowledge gaps according to the optimal laser characteristics which should be used to maximize the healing effects of lasers. The goal of this systematic review and meta-analysis was to determine the effect of photobiomodulation (PBM) as an adjunctive treatment to periodontal therapies to evaluate secondary intention gingival wound healing and post-operative pain. Methods Five databases (PubMed, Embase, Scopus, ProQuest, and Web of Sciences) were searched up to November 30, 2020, for clinical trials that reported the result of the application of PBM on secondary gingival healing wounds and post-operative pain and discomfort after periodontal surgeries. Two independent reviewers selected the eligible studies and the outcomes of interest were extracted. The quality of eligible studies was assessed using the Cochrane Handbook for Systematic Reviews of Interventions. Results Ultimately, twelve studies were included in this review. The application of PBM as an adjunct to periodontal surgeries resulted in a significant improvement in wound healing indices. The Landry wound healing index at the 7th post-operative day was significantly improved (SMD = 1.044 [95% CI 0.62–1.46]; p < 0.01) in PBM + surgery groups compared to the control groups. There was also a statistically significant increase in the complete wound epithelialization (RR = 3.23 [95% CI 1.66–6.31]; p < 0.01) at the 14th post-operative day compared to the control groups. The methods used to assess the post-operative pain were heterogeneous, and therefore the results were limited which made the meta-analysis for post-operative pain assessment not possible. Conclusion Based on the results of this review, PBM can be effectively used as a method to improve secondary intention wound healing. High-quality randomized clinical trials, however, are needed in the future to identify the optimal PBM irradiation parameters and the effect of PBM on post-operative pain.

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