scholarly journals Pertumbuhan dan Kandungan Bahan Bioaktif Selaginella plana dan Selaginellla willdenovii pada Beberapa Media Tanam

2015 ◽  
Vol 1 (1) ◽  
pp. 1-6
Author(s):  
MIFTAHUDIN . ◽  
DWI SUCI SETYANINGSIH ◽  
TATIK CHIKMAWATI
Keyword(s):  
Plant Growth ◽  
Bioactive Compounds ◽  
Rice Husk ◽  
Ethanol Extract ◽  
Bioactive Compound ◽  
Growth Media ◽  
Plant Environment ◽  
Saponin Content ◽  
Two Factors ◽  
Husk Rice

Selaginella, a genus of Pteridophyte, is known as an herb that contains of several bioactive compounds. The growth and bioactive compounds of plant were affected by plant environment, such as media types. The objective of the research was to obtain an appropriate plant media for growing S. plana dan S. willdenovii, and its effect on the content of bioactive compounds. The experiment consisted of two factors, plant growth media and the species of Selaginella. The first factor consisted of eight plant growth media, i.e.: soil, rice husk, rice husk : soil = 1:1 (v/v), burned rice husk, soil : burned rice huks = 1:1 (v/v), burned rice husk : rice husk = 1:1 (v/v), burned rice husk : rice husk = 3:1 (v/v), and burned rice husk : rice husk = 1:3 (v/v). The second factor was two spesies of Selaginella, i.e.: S. plana and S. willdenovii. Ethanol extract of Selaginella was qualitatively analyzed for flavonoid, tannin, and saponin content. The result showed that both fresh and dry weights of Selaginella were influenced by the type of plant media, Selaginella species, and their interactions. S. plana and S. willdenovii grew best on medium containing soil: burned rice = 1:1 (v/v). The highest flavonoid content of S. plana and S. willdenovii was also obtained from the plant grown on the same media. Comparing to the bioactive compound content of natural plant, saponin content of S. plana and S. willdenovii was increased by the treatment of plant media. The treatment also increased tannin content of S. plana, but not of S. willdenovii. 

scholarly journals Micropropagation of Livingstone Potato (Plectranthus esculentus N.E.Br)

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
G. T. Kujeke ◽  
T. C. Chitendera ◽  
R. T. Masekesa ◽  
U. Mazarura ◽  
E. Ngadze ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Block Design ◽  
Growth Media ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Explant Type ◽  
Planting Material ◽  
Two Factors

Livingstone potato (Plectranthus esculentus N.E.Br) is an underutilised indigenous root vegetable grown by communal farmers in the eastern provinces of Zimbabwe. It is vegetatively propagated using unimproved retained tubers from the previous season. The risk of disease carryover is therefore high, leading to poor yields. The objective of the study was to exploit the tissue culture technique of micropropagation to produce a mass supply of healthy planting material for improved productivity. Two experiments were conducted: firstly, to determine the best explant type and secondly, to determine the best landrace and plant growth regulators for the growth of plantlets. The landraces, namely, Ndurwe, Musande, Chibanda, and Chizambezi, were sourced from communal farmers in the stated production areas. Naphthalene acetic acid (NAA) and benzyl amino purine (BAP) were the auxin and cytokinin used, respectively. The first experiment was laid out as a randomized complete block design (RCBD) with two factors: landrace and explant type (shoot tips, nodes, and leaves). After culturing the explants on a plain Murashige Skoog (MS) medium for ten weeks, the best explant was the node with regards to the number of nodes, shoots, and roots of the plantlets which were significant (P<0.05). The second experiment was laid out as a RCBD with two factors: landraces and the plant growth regulator combinations. The nodes were subcultured on an MS medium supplemented with the 16 combinations of plant growth regulators (0 mg/l, 0.5 mg/l, 1 mg/l, and 2 mg/l BAP concentrations: 0 mg/l, 0.2 mg/l, 0.5 mg/l, and 1 mg/l NAA concentrations), respectively. Chizambezi performed best and is, therefore, highly recommended for the rapid multiplication of Livingstone potato. Results from this study have clearly demonstrated that the addition of NAA: BAP at varying concentrations was significant and is essential for optimizing the growth media for micropropagation of Livingstone potato in Zimbabwe. Commercial production of plantlets can, therefore, be carried out to provide healthy planting material for the communal farmers for improved productivity while preserving the germplasm of the underutilised crop at the same time.

scholarly journals The Effects of Root Temperature on Growth, Physiology, and Accumulation of Bioactive Compounds of Agastache rugosa

Agriculture ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 162
Author(s):  
Vu Phong Lam ◽  
Sung Jin Kim ◽  
Gwon Jeong Bok ◽  
Jong Won Lee ◽  
Jong Seok Park
Keyword(s):  
Plant Growth ◽  
Bioactive Compounds ◽  
Defense Mechanism ◽  
Root Zone ◽  
Growth Parameters ◽  
Leaf Gas Exchange ◽  
Bioactive Compound ◽  
Agastache Rugosa ◽  
Root Temperature ◽  
Root Zone Temperature

Plants respond to root temperature stresses by producing antioxidants as a defense mechanism. Since a number of these are phytochemicals with enhancing effects on human health, we examined the effects of 4 root-zone temperature (RZT) treatments (10, 20, 28, and 36 °C) on plant growth and the main bioactive compound concentrations in each organ of Agastache rugosa plants. We aimed to determine the optimal RZT treatment to increase bioactive compound concentrations with no deleterious effects on plant growth. Four-week-old seedlings were grown in a plant factory for 32 days. Nine plant growth parameters, namely, shoot and root fresh weights, stem and root lengths, leaf length and leaf width, leaf area, and shoot and root dry weights were significantly decreased at 10 and 36 °C compared with other treatments. A similar pattern was observed for the chlorophyll content and leaf gas exchange parameters. Of all the RZT treatments, RZT at 28 °C produced the significantly greatest accumulation of two major bioactive compounds, namely, rosmarinic acid (RA) and tilianin contents per the A. rugosa plant, and had no adverse effects on the overall growth of A. rugosa. This supports the use of 28 °C RZT to successfully improve the bioactive compounds with no adverse influence on plant growth or yield.

scholarly journals EFFECTS OF RICE HUSK ASH AND EGGSHELL ON THE GROWTH AND YIELD OF RED CHILI (Capsicum annuum L.)

2018 ◽  
Vol 3 (1) ◽  
pp. 9-15
Author(s):  
Tri Kurniastuti
Keyword(s):  
Plant Growth ◽  
Rice Husk ◽  
Combination Treatment ◽  
Rice Husk Ash ◽  
Fruit Weight ◽  
Growth And Yield ◽  
Plant Yield ◽  
Factor I ◽  
Two Factors ◽  
Number Of Leaves

The purpose of this study was to determine the effect of rice husk ash dosage and eggshell on the growth and yield of red chili, and obtain a treatment that gives the best results on fruit red chili plant. The experiment was arranged with Group Random Design (RAK) with factorial design with two factors and three replications, factor I is dose rice husk ash consists of three levels ie A0: 0 g /plant, A1: 25 gr / plant, A2: 50 g / plant, G3: 75gr / plant. The second factor is the dose eggshell consists of 3 levels ie: C0: 0 g / plant, W1: 15 gr / plant and W2: 30 gr / plant. The experiment will have 9 combination treatment from those 2 factors. The resutls showed that the provision of rice husk ash give the significant effect on height plant growth and the number of leaves, the best dose is the provision of rice husk ash 50 gr / plant. Provision of eggshell give real effect on plant yield of red pepper that is the number of productive branches, number of fruits per plant and fruit weight crop with the best dosage is 30 gr / plant.

scholarly journals Optimization of Indole-3-Acetic Acid Concentration in a Nutrient Solution for Increasing Bioactive Compound Accumulation and Production of Agastache rugosa in a Plant Factory

Agriculture ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 343
Author(s):  
Vu Phong Lam ◽  
Mun Haeng Lee ◽  
Jong Seok Park
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Bioactive Compounds ◽  
Nutrient Solution ◽  
Leaf Gas Exchange ◽  
Bioactive Compound ◽  
Control Treatment ◽  
Gas Exchange Parameters ◽  
Plant Factory ◽  
Indole 3 Acetic Acid

This study aimed to determine the optimal indole-3-acetic acid (IAA) concentration in a nutrient solution to increase the bioactive compounds while enhancing the plant growth of A. rugosa grown hydroponically. Twenty-eight-day-old plants were transplanted in a plant factory for 32 days. The plants were subjected to various IAA concentrations (10−11, 10−9, 10−7, and 10−5 M) from 8 days after transplanting, and the control treatment (without IAA). Shoot and root fresh weights were effectively improved under 10−7 and 10−9 IAA treatments. Leaf gas exchange parameters were increased under 10−7 and 10−9 IAA treatments. Four of the IAA treatments, except 10−11 IAA treatment, significantly increased the rosmarinic acid (RA) concentration, as well as the tilianin concentration was significantly increased at all IAA treatments, compared with that of the control. Especially, the tilianin concentration of the 10−11 IAA treatment was significantly (1.8 times) higher than that of the control. The IAA treatments at 10−5 and 10−7 significantly raised the acacetin concentrations (1.6- and 1.7-times, respectively) compared to those of the control. These results suggested that 10−7 concentration of IAA in a nutrient solution was effective for enhancing plant growth and increasing bioactive compounds in A. rugosa, which offers an effective strategy for increasing phytochemical production in a plant factory.

scholarly journals Pertumbuhan dan Produksi Buncis Tegak (Phaseolus vulgaris) pada beberapa Kombinasi Media Tanam Organik

2013 ◽  
Vol 1 (1) ◽  
pp. 94 ◽  
Author(s):  
Meilya Ramadhiana Safitry ◽  
Juang Gema Kartika
Keyword(s):  
Plant Growth ◽  
Rice Husk ◽  
Agricultural Practices ◽  
Kidney Bean ◽  
Oyster Mushroom ◽  
Environmental Damage ◽  
Growth Media ◽  
Cow Manure ◽  
Paddy Straw ◽  
Straw Mushroom

<p style="text-align: justify;">Media selection is one of important factor for the growth and production of erected kidney bean (Phaseolus vulgarisL.). Utilization the waste media of white oyster mushroom (Pleurotus ostreatus) and waste media of paddy straw mushroom (Volvariella volvaceae), cow manure, and rice husk as plant growth media for kidney bean can be used for optimizing the utilization of local resources and minimizing environmental damage as well as to support the codification of GAP (Good Agricultural Practices) of kidney bean. The research was done from February until October 2011 in experiment field at Leuwikopo, IPB, Bogor. The experiment consisted of one factor and arranged in Randomized Complete Design with thirteen combinations from five kinds of <br />organic media component, they were soil, waste media of white oyster mushroom, waste media of paddy straw mushroom, cow manure, and rice husk with 1:1 ratio (v/v). The kidney bean showed good responses to the combination media treatment of cow manure, combination of soil and waste media of paddy straw mushroom, combination of soil, waste media of paddy straw mushroom, and cow manure, and combination of soil, waste media of paddy straw mushroom, cow manure, and rice husk. How ever the best result of kidney bean production was given from waste media of paddy straw mushroom treatment.</p><p>Keyword:  erected kidney bean, plant growth media, waste media of paddy straw mushroom, co w manure</p>

scholarly journals Quanti-Qualitative Response of Swiss Chard (Beta vulgaris L. var. cycla) to Soil Amendment with Biochar-Compost Mixtures

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 307
Author(s):  
Angela Libutti ◽  
Anna Rita Rivelli
Keyword(s):  
Plant Growth ◽  
Beta Vulgaris ◽  
Interactive Effect ◽  
Organic Amendments ◽  
Experimental Conditions ◽  
Total N ◽  
Qualitative Response ◽  
Positive Effects ◽  
Swiss Chard ◽  
Two Factors

In recent years, soil addition with organic amendments, such as biochar and compost, has gained attention as an effective agronomic practice to sustain soil fertility, enhance plant growth and crop yield. Well known are the positive effects of compost on yield of a wide crop varieties, while both positive and negative responses are reported for biochar Therefore, the aim of the study was to verify the effect of biochar mixed with three types of compost on quanti-qualitative response of Swiss chard (Beta vulgaris L. cycla), a leafy green vegetable rich in dietary antioxidants, largely consumed worldwide. A factorial experiment in pots with two factors, including biochar (without biochar and with biochar from vine pruning residues) and compost (without compost, with compost from olive pomace, with vermicompost from cattle manure, and with compost from cattle anaerobic digestate), was setup. Two growth cycles were considered, and a set of quantitative (height of plants, number, area and fresh weight of leaves) and qualitative parameters (carotenoids, chlorophyll, total N, and NO3−content of leaves) were analyzed. Biochar decreased plant growth and NO3− leaf content; on the contrary, it increased total N leaf content, while compost improved all the considered parameters. The interactive effect of biochar and compost was evident only on total N and NO3− leaf content. In our experimental conditions, the compost showed to be the best option to improve Swiss chard growth and increase the content of phytopigments, while the biochar-compost mixtures did not produce the expected effect.

scholarly journals Comparison of the Phytochemical Properties, Antioxidant Activity and Cytotoxic Effect on HepG2 Cells in Mongolian and Taiwanese Rhubarb Species

Molecules ◽  
2021 ◽  
Vol 26 (5) ◽  
pp. 1217
Author(s):  
Ganbolor Jargalsaikhan ◽  
Jin-Yi Wu ◽  
Yen-Chou Chen ◽  
Ling-Ling Yang ◽  
Ming-Shun Wu
Keyword(s):  
Liver Cancer ◽  
Bioactive Compounds ◽  
Cytotoxic Effect ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Parp Cleavage ◽  
Total Phenolic ◽  
Antioxidant Capacities ◽  
Phytochemical Contents

The Mongolian rhubarb—Rheum undulatum L. (RU)—and Rumex crispus L. (RC)—a Taiwanese local rhubarb belonging to the family of Polygonaceae—are principal therapeutic materials in integrative medicine due to their rich quantities of bioactive compounds; however, their phytochemical and antioxidant properties, and anti-cancer activity is poorly investigated. Furthermore, the phytochemical characteristics of both species may be affected by their different geographical distribution and climatic variance. The current study aimed to compare RU with RC extracts in different polarity solvents (n-hexane, ethyl acetate, acetone, ethanol, and water) for their phytochemical contents including the total phenolic content (TPC), total anthraquinone content (TAC), total flavonoid content (TFC), antioxidant and free radical scavenging capacities, and anticancer ability on the HepG2 cell. Except for the n-hexane extract, all of the RU extracts had considerably higher TPCs than RC extracts, ranging from 8.39 to 11.16 mg gallic acid equivalent (GAE) per gram of dry weight, and the TPCs of each extract were also significantly correlated with their antioxidant capacities by ABTS, DPPH, and FRAP assays (p < 0.05). Moreover, there was no remarkable association between the antioxidant capacities and either TACs or TFCs in both the RU and RC extracts. Besides, high-performance liquid chromatography (HPLC) analysis revealed that both the RU and RC extracts contained chrysophanol, emodin, and physcion, and those bioactive compounds were relatively higher in the n-hexane solvent extracts. Additionally, we observed different levels of dose-dependent cytotoxic effects in all the extracts by cell viability assay. Notably, the ethanol extract of RU had a compelling cytotoxic effect with the lowest half-maximum inhibition concentration (IC50-171.94 ± 6.56 µg/mL at 48 h) among the RU extracts than the ethanol extract of RC. Interestingly, the ethanol extract of RU but not RC significantly induced apoptosis in the human liver cancer cell line, HepG2, with a distinct pattern in caspase-3 activation, resulting in increased PARP cleavage and DNA damage. In summary, Mongolian Rhubarb, RU, showed more phytochemical contents, as well as a higher antioxidant capacity and apoptotic effect to HepG2 than RC; thus, it can be exploited for the proper source of natural antioxidants and liver cancer treatment in further investigation.

Hemp Shives as Natural Amendments in Crops of Rapeseed (Brassica napus) and Flax (Linum usitatissimum)

2018 ◽  
Vol 1 (2) ◽  
pp. 42-52
Author(s):  
Alina Stingu ◽  
Corneliu Tanase ◽  
Valentin I. Popa
Keyword(s):  
Brassica Napus ◽  
Plant Growth ◽  
Linum Usitatissimum ◽  
Growth And Development ◽  
Translocation Factor ◽  
Growth Media ◽  
Plant Growth And Development ◽  
Cadmium Ions ◽  
Cadmium Metal ◽  
Degree Of Recovery

Abstract The aim of this study is to evaluate the possibility of using hemp shives as natural amendments in a phytoremediation sequence. Thus, plant growth tests were conducted for rapeseed (Brassica napus) and flax (Linum usitatissimum) in vegetation pots with sandy soil. These were seeded in a cadmium artificial contaminated environment with or without hemp shives. The efficiency of hemp shives as natural amendments was evaluated after 40 days of initiating experimental model, by evaluation of plant growth and development of plants through biometric and gravimetric measurements. Also, the concentration of assimilatory pigments was determined. In order to establish bioaccumulation capacity, degree of recovery and translocation factor, it was determined the concentration of cadmium metal ions absorbed in various parts of rapeseed and flax plants. It has been found that the development of the root system is more pronounced in the presence of hemp shives both in contamination with cadmium ions and in the absence. By determining cadmium concentrations accumulate in various parts of the plant have been shown that supplementing growth media with hemp shives, improves the bioaccumulation process of cadmium ions. According to the results, the hemp shives can be used as amendments in phytoremediation process, enhancing bioaccumulation process.

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