scholarly journals Effect of surfactant Tween 80 on growth and esterase production of Fusarium culmorum in liquid fermentation

2020 ◽  
Vol 5 (4) ◽  
pp. 64-79
Author(s):  
Henry Medina-Flores ◽  
◽  
Angel González-Márquez ◽  
Carmen Sánchez ◽  
◽  
...  
Keyword(s):  
Esterase Activity ◽  
Enzyme Production ◽  
Culture Media ◽  
Fusarium Culmorum ◽  
Tween 80 ◽  
Ionic Surfactant ◽  
Specific Rate ◽  
Biochemical Tests ◽  
Liquid Fermentation ◽  
Esterase Production

Tween 80 is a widely used non-ionic surfactant that is added to culture media to make hydrophobic substrate available to microorganisms. Because of this surfactant widespread use, it is important to understand how it affects microbial growth and enzyme production. In this work, the effect of different concentrations (100, 400 and 600 l/L; v/v) of Tween 80 (as the sole carbon source) on the biomass production, esterase activities (assessed through biochemical tests and zymographic assays) and protein content of Fusarium culmorum grown in liquid fermentation was determined. The specific growth rate (μ), biomass yield (YX/S), esterase yield (YE/X), esterase productivity (P), maximal enzymatic activity (Emax), and specific rate of enzyme production (qp) were also estimated. A control medium added with glucose was used. The highest μ was showed in the medium added with 100 l of Tween 80/L. However, the greatest esterase activity was observed in those media containing the highest concentrations of Tween 80 ( energy source and it also induced the esterase production by F. culmorum. Tween 80 concentration is positively correlated with the number of esterase isoforms produced by this fungus. The higher the Tween 80 concentration (400 μl/L and 600 μl/L, v/v), the more number of esterase isoforms will be induced. However, lower concentration (100 μl/L) of Tween 80/L did not show a significant effect on the induction of the esterase activity.400 l/L and 600 l/L; v/v). These results show that Tween 80 was used as the sole carbon and

scholarly journals Growth and esterase activity of Fusarium culmorum grown in di(2- ethyl hexyl) phthalate in liquid fermentation

2019 ◽  
Vol 4 (1) ◽  
pp. 51-60 ◽  
Author(s):  
Naomi Surellem Ríos-González ◽  
Ángel González-Márquez ◽  
Carmen Sánchez
Keyword(s):  
Esterase Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Fusarium Culmorum ◽  
Terrestrial Ecosystems ◽  
Phytopathogenic Fungus ◽  
Biochemical Tests ◽  
Liquid Fermentation ◽  
Endocrine Disrupting Compound ◽  
Ethyl Hexyl ◽  
High Concentrations

Di (2-ethyl hexyl) phthalate (DEHP) is a plasticizer present in various products, mainly those made with PVC. This phthalate has attracted attention due to its important participation in the contamination of the environment. It has been reported as an endocrine-disrupting compound in mammals. Fusarium culmorum is a phytopathogenic fungus able to degrade DEHP, because it produces esterases, which are enzymes capable to break down ester bonds present in the structure of phthalates. In this research, growth, protein content and esterases activity by biochemical tests and polyacrylamide gel electrophoresis were characterized for F. culmorum grown in DEHP-supplemented (100 and 1500 mg/L) media as the only carbon source in liquid fermentation. F. culmorum showed higher biomass production and esterase activity in medium supplemented with 1500 mg of DEHP/L. Zymography revealed that bands with esterase activity were observed after 24 h and 48 h in media supplemented with 1500 and 100 mg of DEHP/L, respectively. It was shown that DEHP is an inducer of esterases and that this compound was used as carbon and energy sources by F. culmorum. This fungus can secrete specific esterase to breakdown high concentrations of DEHP, being a promising organism for bioremediation of DEHP-polluted environments in both aquatic and terrestrial ecosystems.

scholarly journals Partial characterization of esterases from Fusarium culmorum grown in media supplemented with di (2-ethyl hexyl phthalate) in solid-state and submerged fermentation

2018 ◽  
Vol 3 (1) ◽  
pp. 82-94 ◽  
Author(s):  
Lorena Ferrer-Parra ◽  
Dolores Itzel López-Nicolás ◽  
Rocío Martínez-Castillo ◽  
Juan Pedro Montiel-Cina ◽  
Ana Rosa Morales-Hernández ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Esterase Activity ◽  
Submerged Fermentation ◽  
Polyacrylamide Gel Electrophoresis ◽  
Fusarium Culmorum ◽  
Phytopathogenic Fungus ◽  
Biochemical Tests ◽  
Enzymatic Production ◽  
High Concentrations

Di (2-ethylhexyl) phthalate (DEHP) is a plasticizer used in the polyvinyl chloride (PVC) industry. The indiscriminate use of various products manufactured with PVC, causes this plasticizer to be considered a contaminant. Fusarium culmorum is a phytopathogenic fungus that has the ability to produce esterase enzymes. Esterases are of great importance because they can break the ester bonds present in the plasticizers. In this work, the activity of esterases produced by F. culmorum grown in media supplemented with different concentrations of DEHP (1500 and 2000 mg/L) in solid-state fermentation and submerged fermentation was characterized by biochemical tests and polyacrylamide gel electrophoresis. F. culmorum showed higher esterase activity in media supplemented with 1500 and 2000 mg DEHP/L in solid-state fermentation. A greater number of esterase activity bands were observed in the DEHP-supplemented media, having a molecular weight of about 20, 25, 37, 45, 55, 75 and 150 kDa, in both fermentation systems. 1500 mg of DEHP/L induced a higher production of esterases, demonstrating that high concentrations of DEHP did not inhibit the enzymatic production of the fungus.

Identification Pseudomonas aeruginosa by OprD Gene for Differentiation from Other Pseudomonas Species that Isolated from Clinical Samples

2019 ◽  
Vol 9 (01) ◽  
pp. 46-50
Author(s):  
Ashwak B Al-Hashimy ◽  
Huda S Alagely ◽  
Akeel K Albuaji ◽  
Khalid R Majeed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
General Hospital ◽  
Culture Media ◽  
Final Diagnosis ◽  
Clinical Samples ◽  
Bacterial Isolates ◽  
Molecular Method ◽  
Biochemical Tests ◽  
Pseudomonas Species ◽  
Specific Sequences

The present study included the collection of 100 samples from various clinical sources for investigating the presence of P. aeruginosa in those sources, the samples have been collected from some hospitals in Baghdad and Hillah city (Al-qassim General Hospital, ,Al-hillah teaching hospital,and Al-hashimya General hospital ) which included wounds, burns, ear and sputum infections. The study was carried out through October 2017 till the end of March 2018. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing or number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to P.aeruginosa bacteria during previous tests based on the OprD gene which has specific sequences for P.aeruginosa bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance . The results of the final diagnosis showed that 38 isolates belong to target bacteria were distributed as 18 of burns, 11 isolates of wounds, 6 isolates of ear infection and 3 isolates of sputum, The examination of the sensitivity of all bacterial isolates was done for elected 38 isolation towards the 9 antibiotic by a Bauer - Kirby and the isolates were resistant for a number of antibiotics used such as Ciprofloxacin 65.7%, Norflaxacin 71%, Imipenem 63.1% Meropenem 68.4%, Gentamicin 65.7%, Amikacin 26.3%, Cefepime 68.4%, Ceftazidime 65.7% and Piperacillin 57.8%.Molecular method , All isolates (38) of P. aeruginosa positive for the diagnostic special gene (OprD) genes (100%).

scholarly journals MOLECULAR DETECTION AND CHARACTERIZATION OF STAPHYLOCOCCUS AUREUS ISOLATED FROM RAW MILK SOLD IN DIFFERENT MARKETS OF BANGLADESH

2017 ◽  
Vol 14 (2) ◽  
pp. 277-282 ◽  
Author(s):  
M. A. Islam ◽  
S. M. L. Kabir ◽  
M. T. Rahman
Keyword(s):  
Molecular Detection ◽  
Culture Media ◽  
Raw Milk ◽  
Rrna Gene ◽  
Biochemical Tests ◽  
Selective Media ◽  
Milk Samples ◽  
Antimicrobial Sensitivity ◽  
Antimicrobial Sensitivity Test

The study was intended for molecular detection of S. aureus isolated from raw cow’s milk. A total of 20 milk samples were collected from different upazila markets of Jamalpur, Tangail, Kishoreganj and Netrokona districts of Bangladesh. Milk samples were cultured onto various culture media for the isolation of bacteria. The isolated bacteria were identified by studying cultural properties on different selective media, biochemical tests, and finally by PCR. Out of 20 samples, 15 (75%) milk samples were found to be positive for S. aureus. S. aureus specific 16S rRNA gene was amplified from all isolates and identified as S. aureus. Antimicrobial sensitivity test was carried out to ascertain the susceptibility of the organism to various antibiotics. Its results showed that the S. aureus isolates were resistant to amoxicillin (100%), erythromycin (73.33%) and tetracycline (73.33%) but sensitive to azithromycin (93.33%), ciprofloxacin (93.33%), gentamicin (100%), norfloxacin (86.67%) and streptomycin (86.67%).

Advantages of fungal enzyme production in solid state over liquid fermentation systems

2003 ◽  
Vol 13 (2-3) ◽  
pp. 157-167 ◽  
Author(s):  
Gustavo Viniegra-González ◽  
Ernesto Favela-Torres ◽  
Cristobal Noe Aguilar ◽  
Sergio de Jesus Rómero-Gomez ◽  
Gerardo Dı́az-Godı́nez ◽  
...  
Keyword(s):  
Solid State ◽  
Enzyme Production ◽  
Liquid Fermentation ◽  
Fungal Enzyme

scholarly journals A New Approach to Producing High Yields of Pulcherrimin from Metschnikowia Yeasts

Fermentation ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 114
Author(s):  
Ewelina Pawlikowska ◽  
Beata Kolesińska ◽  
Maria Nowacka ◽  
Dorota Kregiel
Keyword(s):  
Culture Media ◽  
Growth Control ◽  
Tween 80 ◽  
Resonance Spectroscopy ◽  
Experimental Conditions ◽  
Biofilm Inhibition ◽  
Iron Chelate ◽  
New Approach ◽  
1H Nuclear Magnetic Resonance ◽  
High Yields

Pulcherrimin, a red iron chelate, is produced by some yeasts and bacteria. It plays important ecological roles in many ecosystems, including growth control, biofilm inhibition and photoprotection. In this study, fifteen yeast strains of the genus Metschnikowia were characterized based on their production of pulcherrimin. Yeast pulcherrimin was isolated and its purity assessed using 1H nuclear magnetic resonance spectroscopy. Under experimental conditions, pulcherrimin formation varied depending on both the tested strains and culture media. The best producers formed up to 240 mg/L of pulcherrimin in minimal medium with glucose as the carbon source, supplemented with 0.05% FeCl3 and 0.1% Tween 80. This study presents a new approach to producing high yields of pulcherrimin from yeasts.

scholarly journals MICROBIOLOGICAL STUDY OF ORAL FLORA IN DIABETIC PATIENTS WITH GINGIVITIS

2018 ◽  
Vol 10 (6) ◽  
pp. 113
Author(s):  
Samira Hsaine ◽  
Fatima Zahrae Fethi ◽  
Reda Charof ◽  
Khadija Ounine
Keyword(s):  
Periodontal Disease ◽  
Culture Media ◽  
Bacterial Species ◽  
Klebsiella Oxytoca ◽  
Diabetic Patients ◽  
Periodontal Pathogens ◽  
Biochemical Tests ◽  
Oral Flora ◽  
Metabolic Dysregulation

Objective: Given the importance of the association between diabetes and periodontal disease, the main objective of the present study was to compare the microbial diversity responsible for gingivitis in patients with and without type 2 diabetes.Methods: Samples were collected from the oral cavity of 134 patients with gingivitis and categorised into 3 groups (68 non-diabetic patients and 66 diabetic patients; 33 with controlled diabetes and 33 with poorly controlled diabetes). Sample culture was carried out on selective culture media. The identification of isolated strains involved a series of biochemical tests including miniature galleries (API 20E and 20 Strep), the traditional biochemical gallery (tubes) and automated bacterial identification (BD Phoenix™).Results: Identification by biochemical methods made it possible to differentiate 14 bacterial species and one yeast. There was greater bacterial diversity in diabetic patients as compared to non-diabetic patients. Periodontal pathogens were isolated from both diabetic and non-diabetic populations; however, certain microbes such as Streptococcus acidominimus, Enterobacter cloacae, Klebsiella oxytoca, and Pseudomonas aeruginosa were present only in diabetics, with a much higher percentage in those with poorly controlled diabetes.Conclusion: Poorly controlled diabetes causes metabolic dysregulation that can increase the severity of periodontal disease.

Trichothecene and Moniliformin Production by Fusarium Species from Western Canadian Wheat†

2001 ◽  
Vol 64 (8) ◽  
pp. 1220-1225 ◽  
Author(s):  
D. ABRAMSON ◽  
R. M. CLEAR ◽  
D. GABA ◽  
D. M. SMITH ◽  
S. K. PATRICK ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Liquid Chromatography ◽  
Culture Medium ◽  
Culture Media ◽  
Fusarium Species ◽  
Fusarium Culmorum ◽  
Fusarium Avenaceum ◽  
Gas Chromatography Mass Spectrometry ◽  
Western Canada

Fusarium graminearum, Fusarium culmorum, and Fusarium avenaceum, isolated from Fusarium-damaged wheat harvested in western Canada, were cultured and evaluated for mycotoxin production. Extracts of the culture media were assayed for trichothecenes by gas chromatography-mass spectrometry and for moniliformin by liquid chromatography. Deoxynivalenol (DON) was found in 28 of 42 isolates of F. graminearum and 42 of 42 isolates of F. culmorum at levels ranging from 0.5 to 25.0 μg/g. 15-AcetylDON was found in 28 of 42 isolates of F. graminearum at levels ranging from 1.0 to 7.1 μg/g. 3-AcetylDON was found in 41 of 42 isolates of F. culmorum at levels ranging from 0.8 to 13.0 μg/g. Several other trichothecenes were assayed but not detected in the culture medium. Moniliformin was present in 40 of 42 isolates of F. avenaceum at levels ranging from 1.3 to 138.1 μg/g, but was not present in any of the isolates of F. graminearum or F. culmorum.

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