scholarly journals Perbandingan Aktivitas Antimikroba Ekstrak Etanol dari Tiga Spesies Ganoderma Asal Pulau Lombok

2022 ◽  
Vol 7 (2) ◽  
pp. 160-172
Author(s):  
Faturrahman Faturrahman ◽  
Sukiman Sukiman ◽  
Bambang Fajar Suryadi ◽  
Sarkono Sarkono ◽  
Ernin Hidayati
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Cryptococcus Neoformans ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Sample Collection ◽  
Gram Positive ◽  
Gram Negative ◽  
Microbial Infections ◽  
Ethanol Extracts

The use of antibiotics is one of the most important ways to deal with the spread and treatment of pathogenic microbial infections. The search for new antibiotic sources continues to be carried out to anticipate the emergence of microbial resistance. One of the natural resources that has the potential as an antimicrobial source is a member of the macrofungi of the Genus Ganoderma. The purpose of this study was to evaluate the antimicrobial performance of the ethanol extracts of Ganoderma  lucidum, G. applanatum dan Ganorderma sp. against fungi (Candida albicans dan Cryptococcus neoformans),  gram positive bacteria (Bacillus cereus dan Staphylococcus aureus) and gram negative bacteria (Escherichia coli dan Shigella sp.). Macrofungi samples were taken from the forest area of Nature Tourism Park (TWA) Gunung Tunak, TWA Kerandangan, TWA Suranadi, TWA Nuraksa Sesaot, TWA Lemor and Pusuk forest. The stages of the method performed are sample collection, sample preparation, extraction, and testing of antimicrobial activity using the well difusion method. The ethanol extract concentrations for testing were 20%, 40%, 60% and 80%. The results showed that the three Ganoderma species had anti-fungal and antibacterial activity and that different levels of concentration had an effect on inhibition. The size of the inhibition zone is directly proportional to the higher the extract concentration. The antimicrobial activity of the ethanol extract of G. lucidum was higher when compared to G. applanatum and Ganoderma sp. both against fungi (Candida albicans and Cryptococcus neoformans) as well as against gram-positive and gram-negative test bacteria. In addition, G. applanatum showed very weak inhibition against both groups of tested bacteria.  Key words: candidiasis; cryptococcosis; comorbid infections; macerations; pathogens

scholarly journals PHYTOCHEMICAL INVESTIGATION AND IN VITRO ANTIMICROBIAL SCREENING OF SANTALUM ALBUM SEEDS EXTRACTS

2017 ◽  
Vol 9 (10) ◽  
pp. 117
Author(s):  
Gautam P. Vadnere ◽  
Md. Rageeb Usman ◽  
Santram Lodhi ◽  
Vaishali Patil
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Santalum Album ◽  
Ether Extract ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Screening ◽  
Ethanol Extracts

Objective: Present study aimed phytochemical evaluation and antimicrobial screening of petroleum ether and ethanol extracts of Santalum album seeds.Methods: Petroleum ether and ethanol extracts were screened for presence of chemical constituents. Petroleum ether extract was investigated detail by using chromatographic and spectroscopic methods. In vitro antimicrobial activity of both extracts were investigated using disc diffusion method on two gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus, gram negative Pseudomonas aeruginosa, Escherichia coli and fungus Candida albicans.Results: Santalbic acid was identified in petroleum ether extract and content determined by HPTLC was 4.7%w/w. It was seen that petroleum ether extract have MIC value for B. subtilis, P. aeruginosa, E. coli and C. albicans were 78.125 µg/ml, 19.331 µg/ml, 625 µg/ml & 39.062 µg/ml respectively while MBC was 39.062 µg/ml, 4.882 µg/ml, 312.5 µg/ml & 9.765 µg/ml,  respectively. Petroleum ether extract showed MIC and MBC values for S. aureus was similar as 156.25µg/ml. So, the petroleum ether extract showed significant antimicrobial activity against both gram positive, gram negative and fungal strain.Conclusions: The results of present investigations were indicative of possible high potency of petroleum ether extract due to santalbic acid which could serve as chemotherapeutic agent.

scholarly journals Evaluation of Antimicrobial Potential of the Marine Cyanobacterium, Rivularia mesenterica

2019 ◽  
pp. 1-11 ◽  
Author(s):  
Mirjana Skočibušić ◽  
Sandra Lacić ◽  
Zorica Rašić
Keyword(s):  
Antimicrobial Activity ◽  
Resistant Bacteria ◽  
Gram Negative Bacteria ◽  
Marine Cyanobacterium ◽  
Gram Positive ◽  
Promising Alternative ◽  
Gram Negative ◽  
Antimicrobial Potential ◽  
Ethanol Extracts ◽  
Promising Source

Background: Antibiotic resistance is becoming a pivotal concern for public health accelerating the search for new antimicrobial molecules from nature. The prevention and treatment of infectious diseases by applying products from marine organisms, especially Cyanobacteria as a potential and promising source of antimicrobial agents appears as a possible alternative. Aims: To evaluate the in vitro antimicrobial potential of different extracts derived from marine cyanobacterium Rivularia mesenterica against Gram-positive and Gram-negative bacteria, including multidrug resistant bacteria, by comparison with clinically relevant antibiotics.   Methodology: The secondary metabolites were extracted from fresh and dried cyanobacterial biomass in water and different organic solvents. Antimicrobial efficacy of different extracts was evaluated by the disc diffusion assay. Additionally, the minimum inhibitory concentrations (MIC) of the ethanol extracts obtained from fresh and dried biomass was also determined. Results: The ethanol extracts obtained from fresh and dried biomass of R. mesenterica showed significant antimicrobial activity against five Gram-positive and five antibiotic resistant Gram-negative bacteria and four fungal strains in comparison with the clinically relevant antibiotics. The inhibitory effect of the ethanol extracts was observed, with MIC values in the range 0.06 to 32.00 μg/ml against tested strains. Furthermore, the water extract was inactive against of the tested bacteria and fungi. Conclusion: These results suggest that the ethanol extracts of R. mesenterica possess potent broad spectrum of antimicrobial activity, which can serve as an interesting source for antimicrobial compounds and promising alternative to synthetic antimicrobial drugs discovery.

scholarly journals Antimicrobial Potential of Camellia sinensis against Skin Associated Microbial Pathogens

2019 ◽  
pp. 413-419
Author(s):  
Lovey Sharma ◽  
Ram Kumar Pundir
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Petroleum Ether ◽  
Camellia Sinensis ◽  
Methanolic Extract ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Propionibacterium Acne ◽  
Antimicrobial Potential

The present study was undertaken to assess the antimicrobial potential of leaves’ extract of Green tea (Camellia sinensis) against skin associated microorganisms. The antimicrobial activity was studied through agar well diffusion method against Gram positive, Gram negative bacteria and fungus included Propionibacterium acne, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Candida albicans in five different solvents viz. Methanol, Ethanol, Distilled water, Chloroform and Petroleum ether. The methanolic extract of Camellia sinensis showed maximum zone of inhibition against Propionibacterium acne (27 mm) and S. aureus (26 mm); ethanolic extract was most effective against Candida albicans (22 mm) and petroleum ether extract was most effective against S. aureus (20 mm). The antimicrobial analyses showed that the extracts were more effective against Gram positive bacteria than Gram negative bacteria and fungus. Comparative study of antimicrobial activity of Camellia sinensis extracts with antibiotics revealed that Camellia sinensis methanolic extract had maximum effective antimicrobial activity against all the tested microorganisms. MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) of methanolic extract of Camellia sinensis against the microbial agents under study ranged between 0.125 to 0.015625 mg/ml. The synergistic interaction of Camellia sinensis with antibiotics revealed much better results as compared to antibiotics susceptibility pattern alone. Phytochemicals analyses of Camellia sinensis included alkaloids, saponins, glycosides, proteins, phenols, tannins and phytosterols. The compounds identified by GC-MS analysis of methanolic extract of Camellia sinensis were reported to be used as water treatment, antifungals, antimicrobials and enzymatic inhibitors, improve mental alertness, antifungal, anti-HIV, anticancer, anti-inflammatory, analgesic and comonomer (e.g. for lubricating oil additives). Therefore, it may be concluded that Camellia sinensis leaves possess antibacterial and antifungal properties, which may be used as alternate drug of choice due to its lower side-effects to human skin as compared to presently used therapeutic agents.

40 % aq. HF catalyzed three component Synthesis of novel indeno[1,2-b][1,8]naphthyridin-6(11H)-one derivatives and their antimicrobial activity

2020 ◽  
Vol 17 ◽  
Author(s):  
Srinu Bhoomandla ◽  
Phani Raja Kanuparthy ◽  
Rambabu Gundla ◽  
Ramana Reddy Bobbala
Keyword(s):  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Good Yield ◽  
Diffusion Method ◽  
Subtilis Strain ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Aryl Aldehyde

: A Three component Synthesis of novel 5-phenyl-2-(thiophen-2-yl)-4-(trifluoromethyl)-5H-indeno [1,2-b] [1,8] naphthyridin-6(11H)-one derivatives (4a-n) were prepared using 6-phenyl/(thiophen-2-yl)-4-(trifluoromethyl)pyridin-2-amine, 1H-indene-1,3(2H)-dione and aryl aldehyde using 40% aq. HF with good yield. All the synthesized compounds were screened against Gram-positive and Gram-negative bacterial strains and different Candida strains by well diffusion method. Compounds 4c, 4f and 4g showed promising activity on Bacillus subtilis strain and compounds 4c and 4g showed promising activity towards Candida albicans starains.

scholarly journals Antimicrobial Activity of Plant Extracts againstBacillus Subtilis,Staphylococcus Aureusand Escherichia Coli

2011 ◽  
Vol 8 (s1) ◽  
pp. S282-S284 ◽  
Author(s):  
Liliwirianis N ◽  
Wan Zuraida Wan Mohd Zain ◽  
Jamaluddin Kassim ◽  
Shaikh Abdul Karim
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Stemona Tuberosa ◽  
Elephantopus Scaber

Local herbs have many potential that may be active with antimicrobial activity. A screening was conducted with 11 species of herbs collected in UiTM Pahang Forest Reserve.Epipremnumsp.,Zingibersp.Tetracera indica, Tectaria crenata, Piper stylosum, Homalomena propinque, Goniothalamus sp., Elephantopus scaber, Mapania patiolale, Melastomasp.,Stemona tuberosa, Phullagathis rotundifolia, Thotea grandifoliaandSmilaxsp. were extracted with methanol to obtain their crude. The agar diffusion method using blank disc of 6 mm diameter were loaded with 1000 µg/mL of methanol crude and applied to the inoculate plate was used to assess the antimicrobial activity against two gram positive bacteria (Bacillus subtilisandStaphylococcus aeureus) and one gram negative bacteria (Escherichia coli). The results evaluated as the diameter of the inhibition zone of microbial growth, showed that all the extracts were active against gram-positive bacteria and gram-negative bacteria. The extract ofStemona tuberosewas found to be the most active against theE. coliandS. aeureuswhilePiper stylosumactive againstB. subtilis.

scholarly journals Antibacterial activity of Acanthus sennii extracts against Staphylococcus aureus and Escherichia coli pathogens

2020 ◽  
Vol 13 (2) ◽  
pp. 99-113
Author(s):  
Kindu Geta ◽  
Mulugeta Kibret
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Minimum Bactericidal Concentration ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Microbial Infections ◽  
The Mean ◽  
Ethanol Extracts

Medicinal plants offer a major and accessible source of health care to people living in developing countries. Increasing drug resistant microbial infections intensified the search for new, safer, and more efficacious agents against microbial infections. Acanthus sennii is one of the medicinal plants used traditionally for the treatment of different infectious diseases in Ethiopia. Therefore, this study was carried out to evaluate antibacterial activity of A. sennii against pathogenic bacteria. Plant materials were extracted by maceration technique with chloroform, ethanol and water solvents. The antibacterial activities of the crude extracts of the plant were carried out by the agar well diffusion method. Broth dilution method was used to determine minimum inhibitory and streak plate bactericidal concentration of extracts. The results revealed that ethanol extracts of leaves revealed high antibacterial activity against standard strains of Staphylococcus aureus with inhibition zone of 14±0.6 mm at 25 mg/ml and 17±0.7 mm at 50 mg/ml. Ethanol extracts of buds showed high antibacterial activity against standard strains of S. aureus with inhibition zone of 25.7±0.7 mm at 100 mg/ml, also against standard strains of E. coli with inhibition zone of 16 mm at 50 mg/ml and 23.7 mm at 100 mg/ml. The mean minimum inhibitory concentration of 5.2±1.8 and 2.6±0.5 mg/ml was recorded for ethanol extract of leaves against standard strains of E. coli and clinical isolates of S. aureus; the mean minimum bactericidal concentration of 4.2±1.0 mg/ml with ethanol extract of leaves against standard strains of S. aureus; and the mean minimum bactericidal concentration of 12.5 mg/ml against standard strains and clinical isolates of E. coli. The result showed that A. sennii could be a candidate in the search for new antibacterial agents against these bacteria and its use in ethnomedicinal treatment of infectious diseases used by local communities may be validated. Isolating bio-active components and determining toxicity are future agenda. Keywords: Acanthus sennii, Antibacterial activity, E. coli, Minimum inhibition concentration, Minimum bactericidal concentration, S. aureus  

scholarly journals Evaluation of Antimicrobial Activity of Acacia etbaica Water Extract Leafs against Some Pathogenic Microorganisms

2021 ◽  
Vol 2 (11) ◽  
pp. 1132-1135
Author(s):  
Abdulmageed B Abdullah ◽  
Abdulbaki Al-zaemey ◽  
Rasheed Hasan Mudhesh Al-Husami ◽  
Mofeed Al-Nowihi
Keyword(s):  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Water Extract ◽  
Antimicrobial Effect ◽  
Inhibition Zone ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Inhibition Zones

The antimicrobial activity about water extract of Acacia etbaica was examined by using agar well diffusion methods against five gram-positive and negative bacteria[Staphylococcus aureus (ATCC653-8), Pseudomonas aeruginosa MTCC2453, Bacillus cereus (ATCC6633), Escherichia coli MTCC739, and one local isolate (Staphylococcus epidermides)] in addition to Candida albicans (ATCC2019). this results designated that the water extract of Acacia etbaica possess antimicrobial efficacy against all tested microorganisms either (gram-positive and negative bacteria) or fungi (Candida albicans).Whereas the gram-positive bacteria (S. aureus, S. epidermides and B. cereus) with inhibition zones (21, 19.5 and 16.5) respectively was more sensitive than gram-negative bacteria (P. aeruginosa and E. coli) and C. albicans with inhibition zones 16 and 14.5 respectively. The antimicrobial effect was directly proportional with concentration of water extract where the highest inhibition zone at higher concentration 30% equal 21mm against S.aureus and the lower inhibition zone at lower concentration 5% equal 10mm against C. albicans...

scholarly journals Antibacterial Activity of Rhamnus prinoides extracts against Staphylococcus aureus and Escherichia coli

2019 ◽  
Author(s):  
KINDU GETA
Keyword(s):  
Antibacterial Activity ◽  
Infectious Diseases ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Domestic Animal ◽  
Diffusion Method ◽  
Active Components ◽  
Microbial Infections ◽  
Ethanol Extracts

Abstract Background: Infectious diseases caused by bacteria have a large impact on public health. Increasing in resistant microbial infections intensified the search for new, safer, and more efficacious agents to combat serious microbial infections. Plants have played a central part in combating many diseases in human and domestic animal in many local communities, including Africa. Rhamnus prinoides is Ethiopian medicinal plants that have been used traditionally for the treatment of diverse infectious diseases. Therefore, this research was carried out to evaluate antibacterial activity Rhamnus prinoides extracts against pathogenic bacteria. Methods: The antimicrobial activity tests were carried out by the agar well diffusion method. Broth dilution and streak plate methods were used to determine MIC and MBC of extracts respectively. Results: The results revealed that ethanol extracts of fruits showed the highest antibacterial activity against standard strains of S.aureus with mean inhibition zone of 26±0.58, 28.33±1.2 and 33.33±0.9mm at 25, 50 and 100mg/ml respectively. The lowest mean MIC value (1.04±0.26 mg/ml) was recorded with ethanol extract of fruits against standard strains of S. aureus and the lowest mean MBC value (2.08±0.52 mg/ml) was recorded with ethanol extract of fruits against standard strains and clinical isolates of S. aureu. Conclusion: On the basis of the current findings, Rhamnus prinoides could be a good candidate in the search for new antibacterial agents from natural products against bacterial pathogens. Therefore, further studies are needed to study their toxicology and isolate the bio- active components from this plant.

scholarly journals Antibacterial Activity of Rhamnus prinoides extracts against Staphylococcus aureus and Escherichia coli

2019 ◽  
Author(s):  
kindu geta ◽  
Mulugeta Kibret
Keyword(s):  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Pathogenic Bacteria ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Active Components ◽  
Microbial Infections ◽  
Ethanol Extracts ◽  
Broth Dilution

Abstract Medicinal plants offer a major and accessible source of health care to people living in developing countries. Increasing in resistant microbial infections intensified the search for new, safer, and more efficacious agents to combat serious microbial infections. Acanthus sennii is one of the medicinal plants used traditionally for treatment of different infectious diseases in Ethiopian. Therefore, this research was carried out to evaluate antibacterial activity of Acanthus sennii against pathogenic bacteria. The antimicrobial activity tests of the plant extracts against pathogenic bacteria were carried out by the agar well diffusion method. Broth dilution and streak plate method were used to determine MIC and MBC of extracts respectively. The results revealed that ethanol extracts of leaves showed the highest antibacterial activity against standard strains of S.aureus with inhibition zone of 14±0.58mm and 17±0.67mm at 50mg/ml and at 25mg/ml respectively. Ethanol extracts of buds showed the highest antibacterial activity against standard strains of S.aureus with inhibition zone of 25.67±0.67mm at 100mg/ml. The lowest mean MIC value (2.6±0.52 mg/ml) was recorded with ethanol extract of leaves against standard strains and clinical isolates of S. aureus. The lowest mean MBC value (4.2±1.04 mg/ml) was recorded with ethanol extract of leaves against standard strains of S. aureu. Acanthus sennii could be a good candidate in the search for new antibacterial agents from natural products against bacterial pathogens. Therefore, further studies are needed to study their toxicology and isolate the bio- active components from this plant. Key words: Acanthus sennii, Antibacterial activity, MIC, MBC, pathogenic bacteria

Sign in / Sign up

Export Citation Format

Share Document