scholarly journals Uji Aktivitas Antimikroba Ekstrak n-Butanol Madu Hutan (Apis nigrocincta) dari Kabupaten Selayar dengan Metode KLT-Bioautografi

2020 ◽  
Vol 1 (2) ◽  
pp. 41-45
Author(s):  
A.Suparlan Isya Syamsu
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Staphylococcus Epidermidis ◽  
Salmonella Typhi ◽  
Test Results ◽  
Butanol Extract ◽  
Apis Nigrocincta ◽  
And Staphylococcus Aureus

Preliminary research has been conducted on the antimicrobial activity of n-Butanol extract of forest honey (Apis nigrocincta). This study aims to determine the antimicrobial activity of forest honey from Selayar Regency on the growth of test microbes, using the method of solid dilution with the test microbial Bacillus subtilis, Staphylococcus aureus, Streptococcus mutans, Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, Vibrio sp, Staphylococcus epidermidis, and Candida albicans against n-butanol extract from forest honey (Apis nigrocincta) at 1 mg/ml. The results obtained showed that n-butanol extract inhibited the growth of bacteria Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus aureus. To estimate the compounds that provide antimicrobial activity, the TLC-Bioautography test is performed. Obtained the best results from the separation of compounds by TLC using Chlorophorom eluate: Acetone (3: 1). The TLC-Bioautographic test results showed that the spots with an Rf value of 0.29 gave activity to Bacillus subtilis, Escherichia coli, and Staphylococcus epidermidis, and gave positive results on the appearance of flavonoid compounds.

Synthesis, Characterization and Antimicrobial Activity of Bis-Acetylferrocene Schiff Base

2011 ◽  
Vol 396-398 ◽  
pp. 1875-1878 ◽  
Author(s):  
Yu Ting Liu ◽  
Hai Long Guo ◽  
Da Wei Yin
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Salmonella Typhi ◽  
Bacterial Strains ◽  
Gram Negative ◽  
And Staphylococcus Aureus

Three bis-acetylferrocene schiff bases have been synthesized and characterized by IR, 1H NMR, and elemental analysis, the results conformed well with expected structures. The synthesized compounds were screened in vitro for their antimicrobial activity against three Gram-negative (Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) and two Gram-positive (Bacillus subtilis and Staphylococcus aureus) bacterial strains. The results showed that these compounds are show excellent antimicrobia activities against Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi ,Bacillus subtilis,Staphylococcus aureus.

scholarly journals DAYA ANTIMIKROBA EKSTRAK Lecythophora sp., ENDOFIT YANG DIISOLASI DARI Alyxia reinwardtii

2009 ◽  
Vol 15 (1) ◽  
pp. 37-44
Author(s):  
Noor Erma Sugijanto ◽  
H. Putra ◽  
F. Pritayuni ◽  
N. Albathaty ◽  
Noor Cholies Zaini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Malt Extract ◽  
Streptomycin Sulphate ◽  
Agar Dilution ◽  
And Staphylococcus Aureus

The antimicrobial potential of two endophytic fungi isolated from Alyxia reinwardtii BL, towards selected bacteria (Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Bacillus subtilis FNCC 0059, Pseudomonas aeruginosa ATCC 27853, Salmonella typhi) and fungi (Candida albicans) was tested using ethyl acetate, n-hexane and n-buthanol extracts of fungi cultivated under malt extract liquid fermentation. The extracts were evaluated for its antimicrobial activity by disc diffusion method followed its MIC by agar dilution. Bioautography assay for activity-directed fractionation were also conducted against Bacillus subtilis and Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. Streptomycin sulphate and myconazole used as reference standards. Lecythophora sp. strain 30.1 and 30.5 have broad spectrum antimicrobial activity and a potential source of new classes of antibiotics that could be useful for medicines and biological control agents.

scholarly journals Antimicrobial Activity and Phytochemical Screening of Euphorbia helioscopia

2020 ◽  
Vol 38 ◽  
Author(s):  
K. WAHEED ◽  
S.K. MUHAMMAD ◽  
A. SHOMAILA ◽  
Z. MUHAMMAD ◽  
U. IZHAR ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Water Extract ◽  
Salmonella Typhi ◽  
Fungal Strain ◽  
Phytochemical Screening ◽  
Zone Of Inhibition

ABSTRACT: Medicinal Plants have been used throughout the world by human beings as a drug and remedies for various diseases since time immemorial. A study was planned to count into the antimicrobial activity and phytochemical screening of Euphorbia helioscopia. The plants were gathered and tested against some standard strains and some human pathogenic microorganisms i.e Escherichia coli, Bacillus Subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Pseudomonas aeruginosa and three fungal strain Trichoderma, R hizopus nigricans, Aspergillus niger. The concentrations of extracting samples (500 and 1,000 mg mL-1) were used against pathogens. Ciprofloxacin was used as positive control in case of bacterial strains and Colfrimazol was used against the fungal strain while dimethyl sulfoxide as negative control. The outcomes indicated that the positive wells potency of Water extract had a 36 mm diameter of zone of inhibition against Escherichia coli and ethanol extract at 1,000 mg mL-1 had maximum (34 mm) zone of inhibition against Bacillus subtilus (36 mm) zone of inhibition against Klebsiella pneumonia and 33 mm of zone of inhibition against Trichoderma harzianum. Likewise, water extract at a concentration of 1,000 mg mL-1 resulted highest value of zone of inhibition (36 mm) against Staphylococcus aureus, a zone of inhibition ( mm) against Salmonella typhi, 36 mm zone of inhibition against Pseudomonas aeruginosa, (32 mm) zone of inhibition against Rhizopus nigricans, a 34 mm zone of inhibition against Acremonium and (34 mm) zone of inhibition against Aspergillus niger. The most susceptible bacteria were K. pneumonia and Bacillus subtilis, while E. coli was the most resistant bacteria and showed zone of inhibition. The ethanolic extract had tannins, lipid, total proteins, carbohydrates, flavonoids, Alkaloid and polyphenolics.

scholarly journals Organic management vs. conventional management influence the antimicrobial activity of essential oils of Origanum vulgare L

2020 ◽  
Vol 9 (11) ◽  
pp. e4239118504
Author(s):  
Maysa Mathias Alves Pereira ◽  
Ludmila Caproni Morais ◽  
Natália Jubram Zeneratto ◽  
Willian de Souza Matias Reis ◽  
Omar Cabezas Gómez ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Essential Oils ◽  
Origanum Vulgare ◽  
Family Farmers ◽  
Conventional Management ◽  
Organic Cultivation ◽  
And Staphylococcus Aureus

The objective this stud was to evaluate the antimicrobial activity of essential oils (organic vs. conventional) of Origanum vulgare L. in the action against Candida albicans, Escherichia coli and Staphylococcus aureus. The inoculation of rhizobacteria (environmental (ME) and Bacillus subtilis) potentiated the antimicrobial action.  The essential oils of Traditional (organic) cultivation presented antibacterial action and antifungal, while the essential oil of conventional cultivation (NPK) showed no any antibacterial or antifungal activity. It was concluded that the formulation of vermicomposting and the use of rhizobacteria are potential technologies and tools for family farmers and traditional community in the cultivation of orégano.

scholarly journals ISOLASI DAN UJI ANTIBAKTERI DARI ISOLAT BAKTERI YANG BERASOSIASI DENGAN SPONS Callyspongia aerizusa SERTA IDENTIFIKASI SECARA BIOKIMIA

PHARMACON ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 380
Author(s):  
Angelika Liempepas ◽  
Widya A. Lolo ◽  
Paulina V. Y. Yamlean
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Streptococcus Pyogenes ◽  
Staphylococcus Epidermidis ◽  
Health Sector ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Agar Disc ◽  
And Staphylococcus Aureus

ABSTRACT               Sponge Callyspongia aerizusa contain potential bioactive compound that can be utilized in the health sector. Extract of sea sponge Callyspongia aerizusa, can hamper the growth of Salmonella typhi bacteria, Streptococcus pyogenes, Shigella and Staphylococcus epidermidis. The aim of this study was to test the antibacterial activity against Escherichia coli and Staphylococcus aureus bacteria and identify the type of symbionic bacteria of Callyspongia aerizusa sponge based on their physiological and biochemical characteristics. The method of testing the antibacterial activity was agar diffusion method (Kirby and Baurer diffusion disc). There were three bacterial isolates namely T1, T2, and T3 isolates. The result showed that T1, T2, and T3bacterial isolates had antibacterial activity against Escherichia coli and Staphylococcus aureus test bacteria. Based on the biochemical test, T2bacterial isolates were identified as Bronchothrix bacteria and T1and T3 bacterial identified as Desulfotomaculum. Keywords: Callyspongia aerizusa, Antibacterial activity, symbiont bacteria, Biochemical Identification   ABSTRAKSpons Callyspongia aerizusa memiliki kandungan senyawa bioaktif potensial yang dapat dimanfaatkan dibidang kesehatan. Ekstrak spons laut Callyspongia aerizusa dapat menghambat pertumbuhan bakteri Salmonella typhi, Streptococcus pyogenes, Shigella dan Staphylococcus epidermidis. Penelitian ini bertujuan untuk menguji aktivitas antibakteri dari bakteri simbion spons Callyspongia aerizusa terhadap bakteri Escherichia coli dan Staphylococcuc aureus dan mengidentifikasi jenis bakteri simbion spons Callyspongia aerizusa berdasarkan karakteristik fisiologis dan biokimianya. Metode pengujian aktivitas antibakteri yang digunakan yaitu metode difusi agar (disc diffusion Kirby and Baurer). Terdapat tiga isolat bakteri yaitu isolat T1, T2, dan T3. Hasil penelitian menunjukan bahwa isolat bakteri T1, T2, dan T3 memiliki aktivitas antibakteri terhadap bakteri uji Escherichia coli dan Staphylococcuc aureus. Berdasarkan uji biokima, isolat bakteri T2 diduga sebagai bakteri Brochothrix dan  isolat bakteri T1 dan T3 diduga sebagai bakteri Desulfotomaculum.Kata kunci: Callyspongia aerizusa, Aktivitas antibakteri, Bakteri simbion, Identifikasi Biokimia

Synthesis and Biological Activities of Chalcones Derived from Nitroacetophenone

2012 ◽  
Vol 518-523 ◽  
pp. 255-260 ◽  
Author(s):  
Xiao Yang Qiu ◽  
Su Zhi Li ◽  
An Ran Shi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Trichophyton Rubrum ◽  
Biological Activities ◽  
Tumor Cell Line ◽  
And Staphylococcus Aureus

A series of nitrochalcones (compounds 1-10) were synthesized by reacting appropriate nitroacetophenones and suitable benzaldehydes. The synthesized products were evaluated for their cytotoxic, antibacterial (Bacillus subtilis, Escherichia coli, Pseudomonas fluorescence and Staphylococcus aureus) and antifungal (Aspergillus niger, Candida albicans and Trichophyton rubrum) activities in vitro. Among the compounds tested, (E)-3-(4-fluorophenyl)-1-(3-nitrophenyl)- prop-2-en-1-one (10) showed the favorable in vitro cytotoxic activity against human nasopharyngeal epidermoid tumor cell line KB, and (E)-3-(2-methoxyphenyl)-1-(3-nitrophenyl)prop-2-en-1-one (8) showed the strongest antimicrobial activity with MIC of 20 μg/mL against P. fluorescence.

scholarly journals Preliminary phytochemical screening and susceptibility of bacteria pathogens to whole extract of evolvulus alsinoides (L.)

1970 ◽  
Vol 18 ◽  
pp. 16-20
Author(s):  
BA Omogbai ◽  
FA Eze
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Bacillus Cereus ◽  
Pathogenic Bacteria ◽  
Volatile Oil ◽  
Salmonella Typhi ◽  
Phytochemical Screening ◽  
Evolvulus Alsinoides ◽  
And Staphylococcus Aureus

Context: Plant based antimicrobial represent a vast untapped source for medicines and further exploration of plant antimicrobial neeto occur. Evolvulus alsinoides (L) (Convolvulaceae) is a perennial herb is used in traditional medicine in East Asia, India, Africa and Philippines to cure fever, cough, cold, venereal diseases, azoospermia, adenitis and dementia.   Objective: The objective of this research was to evaluate the antimicrobial activity of the extracts of E. alsinoides on some clinical microbial isolates.   Materials and Methods: The ed thanolic and aqueous extracts of the whole plant (leaves and twigs) were analysed for alkanoids, tannins, glycosides, steroids, flavonoids, saponins, volatile oil and resins. The determination of antibacterial activity was done using the agar well diffusion technique. Pure cultures of pathogenic bacteria such as Bacillus cereus, Staphylococcus aureus, Micrococcus leutus, Klebsiella Pneumoniae, Pseudomonas aeruginosa, Escherichia coli and Salmonella typhi were used for antibacterial activity assay, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).   Results: The ethanolic extract of the plant had MIC values ranging from 16 mg/ml to 512.5 mg/ml. The least MIC was 16mg-ml against Salmonella typhi while Bacillus cereus and Staphylococcus aureus showed the highest MIC of 512.5 mg-ml. In the aqueous extract the MIC ranged between 512.5 to >1025 mg/ml. Salmonella typhi, Micrococcus luteus and Staphylococcus aureus were not inhibited by the water extract. Phytochemical result showed ethanol to be a better solvent for the extraction of the bioactive agents in this plant which include: glycosides, alkaloids, saponins, tannins, flavonoids and volatile oil.   Conclusion: In this study the gram-negative organisms had the lowest MICs and MBCs. This suggests their higher susceptibility to the extract of this plant. On the basis of the result obtained in this investigation it can be concluded that ethanol extract of Evolvulus alsinoides had significant in vitro broad spectrum antimicrobial activity.   Keywords: Evolvulus alsinoides; Phytochemical screening; Antibacterial activity. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8769 JBS 2010; 18(0): 16-20

scholarly journals Anthill Inhibiting Bacteria, a Promising Source of Bio Efficacy

2020 ◽  
pp. 14-21
Author(s):  
A. A. Katun ◽  
A. R. Abdulmumin ◽  
M. U. Yahaya ◽  
N. K. Habeeb ◽  
A. Bala
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Salmonella Typhi ◽  
Biologically Active ◽  
Bacterial Strains ◽  
Active Metabolites ◽  
Bacillus Lentus ◽  
Total Prevalence ◽  
Promising Source ◽  
And Staphylococcus Aureus

The investigation into soil bacteria has been widely studied and becoming increasingly appreciated as an exceptional reservoir of unique naturally occurring biologically active metabolites with pharmaceutical applications. This article aimed to isolate, identify and biochemically characterize antibiotic-producing bacteria from anthill soils in the permanent site of Ibrahim Badamasi Babangida University, Lapai (IBBUL), Niger State, Nigeria. The sum of ten samples were collected from five sampling sites, the sampling was done in threefold (morning, noon and evening) and analyzed adopting standard microbiological protocols. The obtained result revealed that the total bacteria count in the morning ranges from 2.1×107 cfu/mL to 1.4×106 cfu/mL, noon count ranges from 3.1×107 to 2.6×106 cfu/mL while evening count was in the range of 2.1×107 cfu/mL to 1.7×106 cfu/mL. A total number of five (5) bacteria were isolated as Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Bacillus lentus and Micrococcus reseus. The total prevalence of the bacterial isolates in the morning, noon and evening were calculated as B. subtilis (109.08%), S. epidermidis (36.36%), M. reseus (36.36%), B. lentus (63.63%), and S. aureus (54.54%) respectively. These isolates were further assayed against Escherichia coli, Salmonella typhi, Klebsiella sp. and Staphylococcus aureus. The antibacterial outcome showed that two (2) (40%) anthill isolates exhibited antibacterial activity against three (3) tested bacteria (Escherichia coli, Salmonella typhi and Staphylococcus aureus). This research study has showcased that the production of inhibitory substances are common among some of the bacterial strains isolated from anthills.

scholarly journals Combination Effects of Cymbopogon sp. Essential Oil on Selected Bacteria

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Nur Aishah Abdul Wahab ◽  
Hairul Shahril Muhamad ◽  
Nabilah Ahmad Alhadi ◽  
Salina Mat Radzi ◽  
Maryam Mohamed Rehan ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Essential Oils ◽  
Staphylococcus Epidermidis ◽  
Antimicrobial Agents ◽  
Synergistic Effects ◽  
Combination Effects ◽  
Cymbopogon Flexuosus ◽  
Combination Study

Combination effects between Cymbopogon flexuosus and Cymbopogon nardus essential oils were studied to determine whether the combination could emerge as better and more powerful antimicrobial agents against six selected bacteria includes Bacillus subtilis, Escherichia coli, Salmonella typhimurium, Serratia marcescens, Staphylococcus aureus, and Staphylococcus epidermidis. This combination study exhibited 40.67% additive, 28.67% antagonistic, 16.00% indifferent and 14.66% synergistic effects. C. flexuosus and C. nardus essential oils in combination showed a high inhibitory activity against S. aureus with 16% synergistic, 64% additive and 20% indifferent effects.

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