Effect of Extraction Time on Gel Strenght, Viscosity and Amino Acid of Gelatin Fish Bones Pangasius hypopthalmus

Silk proteins have many advantageous components including proteins and pigments. The proteins—sericin and fibroin—have been widely studied for medical applications due to their good physiochemical properties and biological activities. Various strains of cocoon display different compositions such as amino-acid profiles and levels of antioxidant activity. Therefore, the objectives of this study were to find a suitable silk protein extraction method to obtain products with chemical and biological properties suitable as functional foods in two strains of Bombyx mori silk cocoon (Nangsew strains; yellow cocoon) and Samia ricini silk cocoon (Eri strains; white cocoon) extracted by water at 100 °C for 2, 4, 6 and 8 h. The results showed that Nangsew strains extracted for 6 h contained the highest amounts of protein, amino acids, total phenolics (TPC) and total flavonoids (TFC), plus DPPH radical-scavenging activity, ABTS radical scavenging capacity, and ferric reducing antioxidant power (FRAP), anti-glycation, α-amylase and α-glucosidase inhibition. The longer extraction time produced higher concentrations of amino acids, contributing to sweet and umami tastes in both silk strains. It seemed that the bitterness decreased as the extraction time increased, resulting in improvements in the sweetness and umami of silk-protein extracts.

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CONTENT OF AMINO ACID COMPOSITION AND CHANGE OF TENANE IN TEA LEAF IN THE AZERBAIJAN REPUBLIC

chemistry of plant raw material ◽

10.14258/jcprm.2018033415 ◽

2018 ◽

pp. 75-82 ◽

Cited By ~ 2

Author(s):

Мухендис (Mukhendis) Мамедгусейин оглы (Mamedgusejin ogly) Джахангиров (Cahangirov) ◽

Микаил (Mikail) Акпер оглы (Аkper ogly) Магеррамов (Маharrаmov)

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Main Part ◽

Black Tea ◽

Extraction Time ◽

Extraction Temperature ◽

Leaf Processing ◽

High Level ◽

Tea Leaf

It was shown that the scent and taste of tea are determined by the level of protein and amino acid. Certainly, protoingens that make up most part of proteins are noted among amino acids. All of them are considered L-shaped. Green tea mostly consists of proteins. Even the high level protein doesn’t adversely impact the quality of tea yet that of black tea might be negatively influenced by that. Also, its taste will also be worsened by that.As a result of analysis 16 amino acids and 8 unsubstituted substance was found. The main part of amino acid is taken by teanin. It take 41.3% of Azerbaijan-1, 38.8% of Kolxida types respectively. In all stages of tea leaf processing the decrease of teanin and increase of glutamin is witnessed. Also, the loss of teanin happens during the purification (by 34%) and the fading stages.As a result of the work carried out, it has been found that the size of the tea leaf particle, the temperature and the extraction time influence the extraction of the theanine. The optimum are: the size of the tea leaf particle is 200–450 mkm, the extraction temperature is 80–85 °C, and the extraction time is 20–25 minutes.

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Relative bioavailability of phosphorus and true amino acid digestibility by poultry as affected by soybean extraction time and use of low-phytate soybeans

Poultry Science ◽

10.1093/ps/84.10.1555 ◽

2005 ◽

Vol 84 (10) ◽

pp. 1555-1561 ◽

Cited By ~ 5

Author(s):

L.K. Karr-Lilienthal ◽

P.L. Utterback ◽

C. Martinez Amezcua ◽

C.M. Parsons ◽

N.R. Merchen ◽

...

Keyword(s):

Amino Acid ◽

Relative Bioavailability ◽

Extraction Time ◽

Soybean Extraction

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Extraction Optimization and Characterization of Collagen from Yellow Pike Conger Swimbladder with Acid-Hydro-Exctraction Method

Jurnal Pengolahan Hasil Perikanan Indonesia ◽

10.17844/jphpi.v19i2.13461 ◽

2016 ◽

Vol 19 (2) ◽

pp. 156 ◽

Cited By ~ 3

Author(s):

Fernandy Djailani ◽

Win Trilaksani ◽

Tati Nurhayati

Keyword(s):

Amino Acid ◽

Acid Content ◽

Type I Collagen ◽

Amino Acid Content ◽

Extraction Time ◽

Yield Response ◽

Type I ◽

Alternative Source ◽

Extraction Optimization ◽

Ft Ir

Swim bladder is one of marine potential byproducts for alternative source of collagen. This study aimed to optimize hydro-extraction and characterized collagen. Extraction optimization of collagen was determined using Box-behnken design response surface method with three variables: CH3COOH concentration, soaking time and extraction time to yield response. Hydro-extraction collagen was characterization based on the amino acid content, SDS-PAGE, FT-IR and DSC. Acording to the result, the concentration had significantly influence yield. Optimum extraction conditions were variable combinations of acetic acid concentration of 0.1 M, for 1 h and hydro-extraction time of 1 h to produce the highest yield 63.35%. collagen was classified as type I collagen by amino acid content, electrophoresis patterns and fourier transform infrared (FTIR) spectra. The glass transition of collagen was 67.23oC, showed high thermal stability that can be applied to the cosmetics industry and nutraceutical.

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Nutritional Characterization of Sea Bass Processing By-Products

Biomolecules ◽

10.3390/biom10020232 ◽

2020 ◽

Vol 10 (2) ◽

pp. 232 ◽

Cited By ~ 6

Author(s):

Paulo E. S. Munekata ◽

Mirian Pateiro ◽

Rubén Domínguez ◽

Jianjun Zhou ◽

Francisco J. Barba ◽

...

Keyword(s):

Amino Acids ◽

Fatty Acids ◽

Amino Acid ◽

Chemical Composition ◽

Sea Bass ◽

Omega 3 Fatty Acids ◽

Omega 3 ◽

Fish Bones ◽

Promising Source ◽

By Products

The consumption of functional foods and nutraceuticals is gaining more importance in modern society. The exploration of alternative sources and the utilization of by-products coming from the food industry are gaining more importance. The present study aimed to characterize the nutritional value and potential use of sea bass by-products as a source of high-added-value compounds for the development of supplements. The chemical composition (moisture, protein, fat, and ash contents) and profiles of amino acids (high-performance liquid chromatography coupled to a scanning fluorescence detector), fatty acids (gas chromatography coupled to a flame ionization detector), and minerals (inductively coupled plasma optical emission spectroscopy) were determined for sea bass fillet and its by-products (skin, guts, gills, liver, head, and fish bones). The chemical composition assays revealed that by-products were rich sources of proteins (skin; 25.27 g/100 g), fat (guts and liver; 53.12 and 37.25 g/100 g, respectively), and minerals (gills, head, and fish bones; 5.81, 10.11, and 7.51 g/100 g, respectively). Regarding the amino-acid profile, the skin and liver were the main sources of essential amino acids with an essential amino-acid index of 208.22 and 208.07, respectively. In the case of the fatty-acid profile, all by-products displayed high amounts of unsaturated fatty acids, particularly monounsaturated (from 43.46 to 49.33 g/100 g fatty acids) and omega-3 fatty acids (in the range 10.85–14.10 g/100 g fatty acids). Finally, the evaluation of mineral profile indicated high contents of calcium and phosphorus in gills (1382.62 and 742.60 mg/100 g, respectively), head (2507.15 and 1277.01 mg/100 g, respectively), and fish bone (2093.26 and 1166.36 mg/100 g, respectively). Therefore, the main sources of monounsaturated, unsaturated, and long-chain omega-3 fatty acids were guts and liver. The most relevant source of minerals, particularly calcium, phosphorus, and manganese, were head, fish bones, and gills. The most promising source of proteins and amino acids was the skin of sea bass.

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Ultrasonic Extraction for Obtaining IGF-1 from Antler Velvet Residue and Chemical Compositions

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.550-553.1309 ◽

2012 ◽

Vol 550-553 ◽

pp. 1309-1315

Author(s):

Ran Zhou ◽

Fei Wang ◽

Ming Chang ◽

Hong Kun Yue ◽

Shu Fen Li

Keyword(s):

Amino Acid ◽

Total Protein ◽

Extraction Time ◽

Total Amino Acid ◽

Chemical Compositions ◽

Extraction Temperature ◽

Buffer Solution ◽

Pretreatment Condition ◽

Acidic Polysaccharide ◽

Effects Of Ph

In this work, the utilization of ultrasonic technology to extract insulin like growth factor (IGF-1) from antler velvet residue was evaluated. The effects of pH of solvent, the ratio of solvent to sample, extraction temperature and extraction time were investigated through a single factor experiment. The influences of preliminary conditions of supercritical CO2 and co-solvent extraction (SFE) on the activity remaining of IGF-1 in antler velvet residue were discussed. The contents of total protein, total acidic polysaccharide and amino acids were determined, respectively. The results indicate that SFE method has slightly influence on the activity remaining of IGF-1 in antler velvet residue, and the activity remaining of IGF-1 in residue reached 93.68 %. In the optimal pretreatment condition (pH10 buffer solution; the ratio of solvent to sample 20/1 (v/w); extraction temperature 0 °C-35 °C; extraction time 4×15 min), the process yielded 8006.05 ng of IGF-1 per gram antler velvet residue. While the contents of total protein and total acidic polysaccharide were 107.31mg/100mL and 800.7µg/100mL, respectively. Moreover, the total amino acid (TAA) content was up to 120.44mg/100mL and 7 kinds of essential amino acid were 30.08% of the TAA.

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Extraction Optimization and Characterization of Collagen from Yellow Pike Conger Swimbladder with Acid-Hydro-Exctraction Method

Jurnal Pengolahan Hasil Perikanan Indonesia ◽

10.17844/jphpi.v19i2.13223 ◽

2016 ◽

Vol 19 (2) ◽

pp. 156

Author(s):

Fernandy Djailani ◽

Wini Trilaksani ◽

Tati |Nurhayati

Keyword(s):

Amino Acid ◽

Acid Content ◽

Type I Collagen ◽

Amino Acid Content ◽

Extraction Time ◽

Yield Response ◽

Type I ◽

Alternative Source ◽

Extraction Optimization ◽

Ft Ir

Swim bladder is one of marine potential byproducts for alternative source of collagen. This study aimed to optimize hydro-extraction and characterized collagen. Extraction optimization of collagen was determined using Box-behnken design response surface method with three variables: CH3 COOH concentration, soaking time and extraction time to yield response. Hydro-extraction collagen was characterization based on the amino acid content, SDS-PAGE, FT-IR and DSC. Acording to the result, the concentration had significantly influence yield. Optimum extraction conditions were variable combinations of acetic acid concentration of 0.1 M, for 1 h and hydro-extraction time of 1 h to produce the highest yield 63.35%. collagen was classified as type I collagen by amino acid content, electrophoresis patterns and fourier transform infrared (FTIR) spectra. The glass transition of collagen was 67.23o C, showed high thermal stability that can be applied to the cosmetics industry and nutraceutical.

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The staining pattern of the tropomyosin sequence is displayed in a new paracrystal

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100142372 ◽

1986 ◽

Vol 44 ◽

pp. 150-151

Author(s):

M.K. Lamvik ◽

L.L. Klatt

Keyword(s):

Amino Acid ◽

Amino Acid Sequence ◽

Staining Pattern ◽

Banding Patterns ◽

Mass Thickness ◽

New Form

Tropomyosin paracrystals have been used extensively as test specimens and magnification standards due to their clear periodic banding patterns. The paracrystal type discovered by Ohtsuki1 has been of particular interest as a test of unstained specimens because of alternating bands that differ by 50% in mass thickness. While producing specimens of this type, we came across a new paracrystal form. Since this new form displays aligned tropomyosin molecules without the overlaps that are characteristic of the Ohtsuki-type paracrystal, it presents a staining pattern that corresponds to the amino acid sequence of the molecule.

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Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100142311 ◽

1986 ◽

Vol 44 ◽

pp. 134-135

Author(s):

A. J. Tousimis

Keyword(s):

Small Intestine ◽

Amino Acid ◽

Epithelial Cells ◽

Elemental Composition ◽

Isotope Labeling ◽

Structural Components ◽

X Ray ◽

Human Small Intestine ◽

Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

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Immunoelectron microscope detection of C-type natriuretic peptide in normal human atrial tissue

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100147776 ◽

1993 ◽

Vol 51 ◽

pp. 388-389

Author(s):

Chi-Ming Wei ◽

Margaret Hukee ◽

Christopher G.A. McGregor ◽

John C. Burnett

Keyword(s):

Amino Acid ◽

Natriuretic Peptide ◽

Vascular Tone ◽

Cardiac Tissue ◽

Ring Structure ◽

Terminal Amino Acid ◽

Amino Acid Peptide ◽

Normal Human ◽

Terminal Amino ◽

The Brain

C-type natriuretic peptide (CNP) is a newly identified peptide that is structurally related to atrial (ANP) and brain natriuretic peptide (BNP). CNP exists as a 22-amino acid peptide and like ANP and BNP has a 17-amino acid ring formed by a disulfide bond. Unlike these two previously identified cardiac peptides, CNP lacks the COOH-terminal amino acid extension from the ring structure. ANP, BNP and CNP decrease cardiac preload, but unlike ANP and BNP, CNP is not natriuretic. While ANP and BNP have been localized to the heart, recent investigations have failed to detect CNP mRNA in the myocardium although small concentrations of CNP are detectable in the porcine myocardium. While originally localized to the brain, recent investigations have localized CNP to endothelial cells consistent with a paracrine role for CNP in the control of vascular tone. While CNP has been detected in cardiac tissue by radioimmunoassay, no studies have demonstrated CNP localization in normal human heart by immunoelectron microscopy.

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